Efficient preservation of DNA extracted from blood in FTA cards by Chelex method

Chelex resin has been developed and widely used for extracting DNA from different forensic-type samples [1]. However, no studies have shown the conservation of the extracted samples over time in samples from buccal swabs and blood in FTA cards. For this study, we tested three DNA extraction methods: Chelex 10%-Proteinase K (CHK), Phenol Chloroform (PC) and Chelex 10%-Proteinase K purified with Phenol Chloroform (CHK  + PC) in two different kind of samples: blood stored over FTA cards and buccal swab samples.

Source: Forensic Science International: Genetics Supplement Series - October 23, 2019 Category: Forensic Medicine Authors: G. Burgos, R. Flores-Espinoza, V.A. Ruiz-Pozo, I. Villacr és Granda Source Type: research