Internal validation of latest generation str kits for direct str typing from reference samples

Forensic STR kits are manufactured by various biotechnology companies. Differences in primer designs and proprietary chemicals, as well as the availability of instruments in each laboratory, may result in performance differences. As such, internal validation is a necessary step prior to adopting a new kit, especially if accreditation is sought. Here, we evaluated three latest generation direct STR kits (Thermo Fisher Scientific ’s GlobalFiler® Express, Promega’s PowerPlex® Fusion 6C, and Qiagen’s Investigator® 24plex GO!) for direct STR typing of reference samples as part of ISO17025:2017 implementation.

Source: Forensic Science International: Genetics Supplement Series - October 23, 2019 Category: Forensic Medicine Authors: Wannarat Nongmanee, Pirunpat Aitthiprajak, Sukanya Phetpeng, Auttapol Viset, Arjaree Ujchariyakajorn, Nuryanee Sabahanaloh, Sakawrat Aoumtas, Thitika Kitpipit, Phuvadol Thanakiatkrai Source Type: research