Volumetric dried blood spots for determination of phosphatidylethanol: Validation of a liquid chromatography tandem masspectrometry method and clinical application
In conclusion, this study demonstrates that volumetric DBS is a valid specimen for determination of P Eth blood concentrations, offering several advantages. (Source: Drug Testing and Analysis)
Source: Drug Testing and Analysis - April 26, 2024 Category: Drugs & Pharmacology Authors: Olof Beck,
Miguel Barroso,
Sigurd Hermansson,
Cristopher Wid én,
Christer Wallin,
Cecilia Nilsson‐Wallmark,
Andrea de Bejczy Tags: RESEARCH ARTICLE Source Type: research
Cover Picture
(Source: Drug Testing and Analysis)
Source: Drug Testing and Analysis - April 24, 2024 Category: Drugs & Pharmacology Tags: COVER PICTURE Source Type: research
Issue Information
No abstract is available for this article. (Source: Drug Testing and Analysis)
Source: Drug Testing and Analysis - April 24, 2024 Category: Drugs & Pharmacology Tags: ISSUE INFORMATION Source Type: research
Urinary metabolites indicative of the administration of hypoxen monitored by liquid chromatography –high resolution/accurate mass tandem mass spectrometry
First data on suitable urinary markers indicative of the administration of the poly(dihydroxyphenylene) thiosulfonate-based drug hypoxen were generated, which can be employed to support the World Anti-Doping Agency monitoring program. AbstractHypoxen, a poly(dihydroxyphenylene) thiosulfonate-based drug, has been investigated concerning its effect on mitochondrial respiration and the utilization of lactate, especially in the context of strenuous exercise. Since 2023, patterns of use regarding hypoxen amongst the athletic population are monitored by the World Anti-Doping Agency (WADA) and its accredited anti-doping laborator...
Source: Drug Testing and Analysis - April 24, 2024 Category: Drugs & Pharmacology Authors: Christian G örgens,
Tristan Möller,
Sven Guddat,
Erkebulan Svambayev,
Hans Geyer,
Andreas Thomas,
Mario Thevis Tags: SPECIAL ISSUE ‐ SHORT COMMUNICATION Source Type: research
Trends in exposure to drugs and prohibited substances among sports: A nationwide analysis of 2008 –2022 inquiry records
This study aimed to enhance the prevention of sports-prohibited substance misuse by analyzing data collected from major nationwide service systems, enabling the identification of trends in athletes' exposure to drugs and prohibited substances. The study collected over 30,000 data points from three major national anti-doping inquiry systems, spanning from 2008 to 2022. The information of the users consulted products, prohibited substances, and sports disciplines in the data were calculated and categorized. The usage of inquiry systems has shown an increasing trend from 2008 to 2022. Athletes comprised the majority of users ...
Source: Drug Testing and Analysis - April 24, 2024 Category: Drugs & Pharmacology Authors: Cheng ‐Chia Chen,
Mei‐Chich Hsu,
Yu‐Tse Wu,
Chi‐Hua Chen,
Tzu‐Yu Lin,
Wei Ho,
William Chih‐Wei Chang Tags: RESEARCH ARTICLE Source Type: research
Methylation of phase II metabolites of endogenous anabolic androgenic steroids to improve analytical performance
Intact phase II metabolites of endogenous anabolic androgenic steroids give important information about metabolism and have the potential to improve the detection of doping with testosterone. Currently applied methods for liquid chromatography –mass spectrometry analysis include chemical derivatization at the steroid moiety, which can pose challenges regarding chromatographic performance, for example, poor peak shape. Methylation with trimethylsilyl-diazomethane at the acidic moiety of the phase II conjugate improves analytical performa nce by the formation of narrower and more intense peaks. AbstractThe study of intact ...
Source: Drug Testing and Analysis - April 22, 2024 Category: Drugs & Pharmacology Authors: Sandra Pfeffer,
Guenter Gmeiner,
Guro Forsdahl Tags: RESEARCH ARTICLE Source Type: research
Association of Official Racing Chemists guidelines for drug testing in animal hair for doping control
The Association of Official Racing Chemists (AORC) guidelines for drug testing in animal hair provide animal sport doping control laboratories with a framework for the implementation of a robust and legally defensible program for the analysis, both screening and confirmatory, of animal hair samples. AbstractThe Association of Official Racing Chemists (AORC) guidelines for drug testing in animal hair provide animal sport doping control laboratories with a framework for the implementation of a robust and legally defensible program for the analysis, both screening and confirmatory, of animal hair samples. The guidelines were ...
Source: Drug Testing and Analysis - April 19, 2024 Category: Drugs & Pharmacology Authors: Bob Gray,
Ludovic Bailly ‐Chouriberry,
Wai Him Kwok,
Sean Yamada,
Masayuki Yamada,
Benjamin Moeller Tags: TUTORIAL Source Type: research
Development of a method to evaluate the effects of external environments on drug stability in nails using micro ‐segmental analysis
An evaluation method using micro-segmental analysis (MSA) of a single nail was developed to examine the effects of external environments on drug stability in nails. MSA is indispensable for examining various experimental conditions and maintaining the original nail shapes from a limited number of reference nail samples. AbstractNails can be used as an alternative to hair for examining past drug use. However, daily hand-and-nail care can eliminate the internal drugs. Therefore, we developed an evaluation method to examine the effects of the external environment on drug stability in nails using micro-segmental analysis. Firs...
Source: Drug Testing and Analysis - April 18, 2024 Category: Drugs & Pharmacology Authors: Kenji Kuwayama,
Hajime Miyaguchi,
Tatsuyuki Kanamori,
Kenji Tsujikawa,
Tadashi Yamamuro,
Hiroki Segawa,
Yuki Okada,
Yuko T. Iwata Tags: RESEARCH ARTICLE Source Type: research
Development of 3 β,4α‐dihydroxy‐5α‐androstan‐17‐one standard solution for doping analyses
In this work, a certified reference material (CRM) for 3β,4α-dihydroxy-5α-androstan-17-one (DHAS), a metabolite of formestane that is used to conceal prohibited anabolic steroids, in methanol solution (NMIJ CRM 6212-a) was developed. To evaluate certified value of the CRM traceable to the International System of Units (SI), we newly applied different analytical methods, quantitative NMR (qNMR) and a combination of qNMR and high-performance liquid chromatography (qNMR/HPLC) with an SI-traceable internal standard for quantitative NMR (qNMR). AbstractDoping analyses are essential for sporting events because some athletes m...
Source: Drug Testing and Analysis - April 12, 2024 Category: Drugs & Pharmacology Authors: Miho Kuroe,
Taichi Yamazaki,
Naoki Saito,
Yoshitaka Shimizu,
Toshihide Ihara,
Masahiko Numata,
Nobuyasu Itoh Tags: RESEARCH ARTICLE Source Type: research
Quantitative determination of R/S ‐methadone in human serum using ultra‐high performance supercritical fluid chromatography‐tandem mass spectrometry: A method for routine use
A method for separation and quantitation of R- and S-methadone is developed and validated using ultra-high performance supercritical fluid chromatography-tandem mass spectrometry. This is a rapid method suitable for routine use in therapeutic drug monitoring and is currently being used at our laboratory for authentic patient samples. AbstractMethadone has two enantiomers, which exhibit differences in pharmacological effects, with R-methadone being the active and S-methadone the inactive enantiomer. A robust, simple and rapid method for chiral separation of the two enantiomers in serum samples using ultra-high performance s...
Source: Drug Testing and Analysis - April 11, 2024 Category: Drugs & Pharmacology Authors: Miriam Hansen,
Hilde Havnen,
Trine Naalsund Andreassen,
Olav Spigset,
Solfrid Hegstad Tags: RESEARCH ARTICLE Source Type: research
Alternative routine for reporting chiral amphetamine test results in assessment of attention ‐deficit/hyperactivity disorder medication: experiences from 2013 to 2023
In conclusion, this study demonstrated the clinical value and utility of a new routine for reporting urinary chiral amphetamine results to differentiate adherence to ADHD medication from illicit drug use. Unlike thel/d-amphetamine ratio, it considers differences in total amphetamine concentration and urine dilution, factors that can affect the interpretation. (Source: Drug Testing and Analysis)
Source: Drug Testing and Analysis - April 11, 2024 Category: Drugs & Pharmacology Authors: Anders Helander,
Annika Andersson,
Tomas Vill én Tags: RESEARCH ARTICLE Source Type: research
A direct analysis method using sheath flow probe electrospray ionisation ‐mass spectrometry (sfPESI‐MS) to detect drug residues from fingerprint forensic gel lifts
This study demonstrates the application of an ambient ionisation method, sheath flow probe electrospray ionisation-mass spectrometry (sfPESI-MS), to the direct analysis of gel-lifted fingerprints. A model drug compound (zolpidem) is successfully detected from gel-lifted prints from three different surface types: glass, metal, and paper. The surface activity-based separation associated with probe electrospray approaches is shown to resolve zolpidem ions from background phthalate species, significantly enhancing the response obtained from the gel-lifter. A depletion series experiment shows that the drug residue can be detect...
Source: Drug Testing and Analysis - April 9, 2024 Category: Drugs & Pharmacology Authors: Ayoung Kim,
Paul F. Kelly,
Matthew A. Turner,
James C. Reynolds Tags: SPECIAL ISSUE ‐ RESEARCH ARTICLE Source Type: research
Plasma drug screening using paper spray mass spectrometry with integrated solid phase extraction
AbstractDrug overdoses have risen dramatically in recent years. We developed a simple nontargeted method using a disposable paper spray cartridge with an integrated solid phase extraction column. This method was used to screen for ~160 fentanyl analogs, synthetic cannabinoids, other synthetic drugs, and traditional drugs of abuse in over 300 authentic overdose samples collected at emergency departments in Indianapolis. A solid phase extraction step was implemented on the paper spray cartridge to enable subnanograms per milliliter synthetic drugs screening in plasma. Analysis was performed on a quadrupole orbitrap mass spec...
Source: Drug Testing and Analysis - April 8, 2024 Category: Drugs & Pharmacology Authors: Hannah Zimmerman ‐Federle,
Greta Ren,
Sarah Dowling,
Cassandra Warren,
Daniel Rusyniak,
Robert Avera,
Nicholas E. Manicke Tags: SPECIAL ISSUE ‐ RESEARCH ARTICLE Source Type: research
Arecoline in buccal cells reflects Areca nut dose
We found robust relationships between a chewer'sAreca nut (AN) exposure, expressed as their average AN load, and their buccal cell arecoline levels. Therefore, we suggest buccal cell arecoline to be a reliable biomarker that reflects the extent of AN chewing. Our data are useful for future AN cessation studies and the determination of AN chewing status from studies with limited self-reported data. AbstractAreca nut (AN) is a carcinogen; its chewing cessation is, therefore, of worldwide interest. However, cessation biomarkers are lacking. We sought to establish arecoline in chewers' buccal cells (BCs) as a biomarker for AN ...
Source: Drug Testing and Analysis - April 8, 2024 Category: Drugs & Pharmacology Authors: Jennifer F. Lai,
Ana Joy Mendez,
Xingnan Li,
Lynne R. Wilkens,
Thaddeus Herzog,
Adrian A. Franke Tags: RESEARCH ARTICLE Source Type: research
Simultaneous quantitation and identification of intact Nandrolone phase II oxo ‐metabolites based on derivatization and inject LC–MS/(HRMS) methodology
Accurate and convenient analysis of the intact phase II oxo-metabolites of nandrolone under the provisions of WADA's TD2023IDCR was accomplished with a dilute derivatization reagent (Girard's reagent T) and inject strategy. AbstractΑ sensitive and selective derivatization and inject method for the quantification of intact nandrolone phase II oxo-metabolites was developed and validated using liquid chromatography - (tandem high resolution) mass spectrometry (LC–MS/(HRMS)). For the derivatization, Girard's reagent T (GRT) was used directly in natural urine samples and the analysis of the metabolites of interest was perfor...
Source: Drug Testing and Analysis - April 7, 2024 Category: Drugs & Pharmacology Authors: Panagiotis Sakellariou,
Polyxeni Kiousi,
Michael Petrou,
Yiannis S. Angelis Tags: RESEARCH ARTICLE Source Type: research
