Development of 3 β,4α‐dihydroxy‐5α‐androstan‐17‐one standard solution for doping analyses

In this work, a certified reference material (CRM) for 3β,4α-dihydroxy-5α-androstan-17-one (DHAS), a metabolite of formestane that is used to conceal prohibited anabolic steroids, in methanol solution (NMIJ CRM 6212-a) was developed. To evaluate certified value of the CRM traceable to the International System of Units (SI), we newly applied different analytical methods, quantitative NMR (qNMR) and a combination of qNMR and high-performance liquid chromatography (qNMR/HPLC) with an SI-traceable internal standard for quantitative NMR (qNMR). AbstractDoping analyses are essential for sporting events because some athletes might use prohibited substances to win games. To obtain reliable results from doping analyses, it is important to use both reliable standard solutions and validated analytical methods at accredited laboratories. Among the focused compounds related to prohibited substances listed by the World Anti-Doping Agency, we developed a certified reference material (CRM) for 3β,4α-dihydroxy-5α-androstan-17-one (DHAS), a metabolite of formestane that is used to conceal prohibited anabolic steroids, in methanol solution (NMIJ CRM 6212-a). To develop a CRM traceable to the International System of Units (SI), we newly applied different analytical methods with an SI-traceable internal standard for quantitative NMR (qNMR) instead of mass balance approach because this CRM solution was required to develop rapidly using a limited amount of high-purity DHAS. One method was gra...
Source: Drug Testing and Analysis - Category: Drugs & Pharmacology Authors: Tags: RESEARCH ARTICLE Source Type: research