Cannabis profiling of seized samples: An intra ‐location variability study using gas chromatography–mass spectrometry profiles and multivariate data analysis
A cannabis profiling approach was developed on a large and representative data set, combining statistics with multivariate data analysis. Based on the chemical profiles, the intra (within)- and inter (between)-location variabilities were studied. Different data pretreatments were evaluated on their discriminating power by calculating the false positive (FP) error rate. This manuscript indicated the large influence of data preprocessing with a significant decrease in FPs, but still not till a level acceptable to defend in court. AbstractYearly, cannabis belongs to the most seized drugs worldwide. During judicial investigati...
Source: Drug Testing and Analysis - September 11, 2023 Category: Drugs & Pharmacology Authors: Amorn Slosse,
Filip Van Durme,
Nele Samyn,
Debby Mangelings,
Yvan Vander Heyden Tags: RESEARCH ARTICLE Source Type: research
Insulin ‐mimetic peptides in sports drug testing
Peptide-based drugs that own insulin mimetic properties reach the focus of sports drug testing. The present study enables the determination including their in vitro derived metabolites by means of LC-HRMS. AbstractBecause of its influence on carbohydrate metabolism and, at the same time, anti-catabolic effects, the misuse of the peptide hormone insulin and its synthetic analogs is prohibited in sports at all times according to the regulations of the World Anti-Doping Agency (WADA). The biological effects of insulin and its analogs are mediated through binding to the insulin receptor, which was also found to be activated by...
Source: Drug Testing and Analysis - September 11, 2023 Category: Drugs & Pharmacology Authors: Andreas Thomas,
Sophia Krombholz,
Johanna Breuer,
Katja Walpurgis,
Mario Thevis Tags: RESEARCH ARTICLE Source Type: research
Detection of capromorelin in urine following oral and dermal routes of administration
Exposure to a capromorelin-based veterinary drug has been demonstrated to result in measurable urinary excretion of capromorelin for at least 48 h, from less than one to a few nanograms per milliliter, depending on administration route (oral or transdermal). AbstractCapromorelin is a growth hormone secretagogue. Despite promising results to alleviate muscle-wasting in the elderly, it has not advanced further in human development. Subsequent studies demonstrated capromorelin's ability to increase food intake in animals, leading to approval in the United States and Europe as an appetite stimulant for cats (Elura) and dogs...
Source: Drug Testing and Analysis - September 9, 2023 Category: Drugs & Pharmacology Authors: Tim Sobolevsky,
Katja Walpurgis,
Christian Goergens,
Matthew Fedoruk,
Laura Lewis,
Brian Ahrens,
Mario Thevis Tags: SHORT COMMUNICATION Source Type: research
Detection of adeno ‐associated viral DNA in equine post‐administration frozen blood and plasma samples after long‐term storage
This study provides confidence in the detection of gene doping material when re-analysing samples and/or analysing ‘B’ samples for confirmatory analysis. AbstractGene doping in horses is a threat to the fairness in sport and has serious implications for animal welfare. To investigate the effect of long-term storage on the detection of AAV in plasma and whole blood, samples from an administration study using an adeno-associated virus serotype 6 expressing green fluorescence protein (AAV6-GFP) were stored at −20°C for 8 months before analysis. The AAV vector was detected in stored plasma samples, following the same d...
Source: Drug Testing and Analysis - September 7, 2023 Category: Drugs & Pharmacology Authors: Jillian Maniego,
Bogumila Pesko,
Jocelyn Habershon ‐Butcher,
Pamela Hincks,
Polly Taylor,
Graham Stewart,
Christopher Proudman,
Edward Ryder Tags: SHORT COMMUNICATION Source Type: research
Production and engineering of nanobody ‐based quenchbody sensors for detecting recombinant human growth hormone and its isoforms
This study focuses on the development and improvement of a simple and fast rhGH detection method using a fluorescence-incorporated antibody sensor “Quenchbody (Q-body)” that activates upon antigen binding. Camelid-derived nanobodies were used to produce stable Q-bodies that withstand high temperatures and pH levels. Notably, pituitary human growth hormone (phGH) comprises two major isoforms, namely 22 and 20 kDa GH, which exist in a spec ific ratio, and the rhGH variant shares the same sequence as the 22 kDa GH isoform. Therefore, we aimed to discriminate rhGH abuse by analyzing its specific isoform ratio. Two nano...
Source: Drug Testing and Analysis - September 5, 2023 Category: Drugs & Pharmacology Authors: Jaehoon Jung,
Yujin Jeong,
Yinglan Xu,
Joonyeop Yi,
Minyoung Kim,
Hee ‐Jin Jeong,
Sang Hoon Shin,
Yung‐Hun Yang,
Junghyun Son,
Changmin Sung Tags: SHORT COMMUNICATION Source Type: research
Applications of biomarkers of exposure and biological effects in users of new generation tobacco and nicotine products: Tentative proposals
The authors describe recent progress in developing biomarkers that distinguish between exposure to tobacco smoke versus a range of nicotine products, including harm reduction products. They also highlight advances in measuring biomarkers that are predictive of future disease outcomes. This progress has implications for conducting more rigorous epidemiological studies and for insurance companies approaches to underwriting. AbstractDespite efforts to eliminate smoking, more than 1 billion people worldwide continue to use combustible cigarettes through choice or inability to quit. With an associated 8 million deaths, the prov...
Source: Drug Testing and Analysis - September 1, 2023 Category: Drugs & Pharmacology Authors: Derek Yach,
Gerhard Scherer Tags: PERSPECTIVE Source Type: research
Quantification of (9R) ‐ and (9S)‐hexahydrocannabinol (HHC) via GC–MS in serum/plasma samples from drivers suspected of cannabis consumption and immunological detection of HHC and related substances in serum, urine, and saliva
In this study, a GC –MS method for the quantification of Δ9-tetrahydrocannabinol (THC), 11-hydroxy-Δ9-tetrahydrocannabinol (11-OH-THC), and 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH) was extended to (9R)- and (9S)-HHC. The applicability was proven by serum/plasma samples from drivers suspected of cannabis consumption. Limit of detection (LOD) and lower limit of quantification (LLOQ) were 0.15 and 0.25 ng/mL, respectively. Within-run imprecision was<6.5% and between-run imprecision was<10.0%. Inter-injection stability, processed sample stability (3 days), freeze–thaw stability (three cycles), and...
Source: Drug Testing and Analysis - September 1, 2023 Category: Drugs & Pharmacology Authors: Lisa H öfert,
Susen Becker,
Jan Dreßler,
Sven Baumann Tags: RESEARCH ARTICLE Source Type: research
