Comprehensive metabolic investigation of dopamine reuptake inhibitor HDMP ‐28 in equine liver microsomes and Cunninghamella elegans for doping control
This study explores the metabolic conversion of HDMP-28 mediated by equine liver microsomes andCunninghamella elegans. An extraction and detection method was developed, optimized, and validated for doping assessment in equine urine and plasma. Liquid chromatography –high-resolution mass spectrometry was employed to determine metabolite structures. The study identified 31 (22 phase I and 9 phase II) metabolites of HDMP-28, including hydroxylated, hydrogenated, and hydrolyzed analogs. Glucuronic acid-conjugated metabolites were also detected. This manuscript d escribes metabolites based on the in vitro studies, which might...
Source: Drug Testing and Analysis - January 16, 2024 Category: Drugs & Pharmacology Authors: Meleparappil Muhammed Ajeebsanu,
Michael Benedict Subhahar,
Abdul Khader Karakka Kal,
Moses Philip,
Zubair Perwad,
Tajudheen Kunhamu Karatt,
Fatma Mohammed Graiban,
Marina Joseph,
Shantymol V. Jose Tags: RESEARCH ARTICLE Source Type: research
Detection of FG ‐4592 and metabolites in equine plasma, urine and hair following oral administration
Hypoxia-inducible factor inhibitors, including FG-4592, are prohibited in sports, and thus identifying the best targets for their detection in doping control is essential. Analysis of equine urine, plasma and hair showed that the parent drug was the best target for detection of oral administration in all three matrices. AbstractFG-4592 is a hypoxia-inducible factor inhibitor that has been approved for therapeutic use in some countries. This class of compounds can increase the oxygen carrying capacity of the blood and thus have the potential to be used as performance enhancing agents in sports. The purpose of this study was...
Source: Drug Testing and Analysis - January 13, 2024 Category: Drugs & Pharmacology Authors: Charlotte Cutler,
Marjaana Viljanto,
Polly Taylor,
Pamela Hincks,
Jocelyn Habershon ‐Butcher,
Bobby Gray,
James Scarth Tags: RESEARCH ARTICLE Source Type: research
Comparison of analytical approaches for the detection of oral testosterone undecanoate administration in men
Urine and serum samples of an oral testosterone undecanoate (TU) administration study were analysed to detect the testosterone administration. The 5 αAdiol/E was the most indicative marker of the urinary steroid profile for volunteers with naturally low T/E ratios. The direct detection of TU in serum was an efficient alternative to urinary isotope ratio mass spectrometry (IRMS) analysis, although with a slightly shorter detection window. AbstractFor antidoping laboratories, the determination of an illicit testosterone (T) administration in urine samples remains a difficult process as it requires the determination of the e...
Source: Drug Testing and Analysis - January 12, 2024 Category: Drugs & Pharmacology Authors: Tobias Langer,
Raul Nicoli,
Carine Schweizer ‐Grundisch,
Silke Grabherr,
Tiia Kuuranne,
Alessandro Musenga Tags: RESEARCH ARTICLE Source Type: research
Identification of three unexpected new psychoactive substances at an Australian drug checking service
Drug checking is a herm reduction measure allowing the public to test the content and purity of drugs before consumption that can also serve as an effective way to monitor the unregulated drug market. In June 2023, three samples were presented to the CanTEST Health and Drug Checking Service located in Canberra, Australia. In each case, analysis revealed substances that did not meet client expectation and were instead identified as previously undocumented new psychoactive substances. AbstractDrug checking is a harm reduction measure that provides people with the opportunity to confirm the identity and purity of substances b...
Source: Drug Testing and Analysis - January 11, 2024 Category: Drugs & Pharmacology Authors: Jess L. Algar,
Douglas J. Lawes,
Adam J. Carroll,
David Caldicott,
Malcolm D. McLeod Tags: RESEARCH ARTICLE Source Type: research
Amphetamine ‐type drug‐related fatalities in the context of 8 years of methamphetamine use measured by wastewater analysis in South Australia
This study shows that the response to increasing methamphetamine use in South Australia h as resulted in a reversal of the upward trend in consumption and fewer drug-related fatalities. (Source: Drug Testing and Analysis)
Source: Drug Testing and Analysis - January 11, 2024 Category: Drugs & Pharmacology Authors: Emma L. Jaunay,
Bradley S. Simpson,
Jason M. White,
Cobus Gerber Tags: RESEARCH ARTICLE Source Type: research
Influence of ZnO morphology on the capability of portable paper ‐based electrospray ionization mass spectrometry to determine therapeutic drugs in complex matrices
In this study, PPESI-MS was used to demonstrate the effect of different morphologies of zinc oxide on the analytical sensitivity of drugs in complex samples. Among them, flower-like zinc oxide prepared using ultrasonic technique exhibited a superior analytical performance compared to other morphologies. AbstractAdsorbents play a significant role in enhancing the analytical sensitivity of target analytes in complex samples by mitigating matrix effects. In our recent report, ZnO stood out among various adsorbents to determine target therapeutic drugs in complex biological matrices when applied for portable paper-based ele...
Source: Drug Testing and Analysis - January 9, 2024 Category: Drugs & Pharmacology Authors: Yan Zhang,
Yajie Huang,
Yun Li,
Zhiming Zhang,
Qianqian Zuo,
Yajun Zheng,
Zhiping Zhang Tags: RESEARCH ARTICLE Source Type: research
Prevalence of carboxy ‐Δ8‐tetrahydrocannabiniol in antidoping samples
This study aims to estimate the prevalence of Δ8-THC use in the athlete community by monitoring for Δ8-THC-COOH in samples collected for antidoping. A high-performance liquid chromatography tandem mass spectrometry (LC –MS/MS) method was utilized to resolve Δ8 and Δ9-THC-COOH. One thousand samples with a presumptive Δ9-THC-COOH finding in routine screening were analyzed by the above LC –MS/MS method. Approximately 12% of samples contained Δ8-THC-COOH at relative abundances between 5% and 100% of total carboxy-THC content. (Source: Drug Testing and Analysis)
Source: Drug Testing and Analysis - January 5, 2024 Category: Drugs & Pharmacology Authors: Vinod S. Nair,
Mari Heybroek,
Emily Boyle,
Mason Rogers,
Thane Campbell,
Daniel Eichner,
Kevin Hill Tags: RESEARCH ARTICLE Source Type: research
Influence of carbon side chain length on the in vivo pharmacokinetic and pharmacodynamic characteristics of illicitly manufactured fentanyls
Five non-pharmaceutical fentanyls (NPF) were tested in a rat model to determine pharmacokinetic and pharmacodynamic profile differences. Fentanyl displayed the greatest analgesic and hypothermic effect of all compounds. Acetylfentanyl was the only compound that displayed pharmacokinetic differences from the other NPF, with a shorter half-life, area under the curve and maximum concentration. AbstractSince 2016, illicitly manufactured fentanyls and fentanyl analogs (referred to as IMFs) have contributed to an increase in drug overdoses. Although fentanyl has been characterized and evaluated extensively in animals and humans,...
Source: Drug Testing and Analysis - December 31, 2023 Category: Drugs & Pharmacology Authors: Jeremy R. Canfield,
Jon E. Sprague Tags: RESEARCH ARTICLE Source Type: research
