Development of a rapid and specific MALDI-TOF mass spectrometric assay for SARS-CoV-2 detection
AbstractWe have developed a rapid and highly specific assay for detecting and monitoring SARS-CoV-2 infections by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). As MALDI-TOF mass spectrometers are available in a clinical setting, our assay has the potential to serve as alternative to the commonly used reverse transcriptase quantitative polymerase chain reaction (RT-qPCR). Sample preparation prior to MALDI-TOF-MS involves the tryptic digestion of SARS-CoV-2 proteins, followed by an enrichment of virus-specific peptides from SARS-CoV-2 nucleoprotein via magnetic antibody beads. O...
Source: Clinical Proteomics - July 1, 2023 Category: Biochemistry Source Type: research
VCAM-1 complements CA-125 in detecting recurrent ovarian cancer
ConclusionsA two-marker panel of CA-125 and VCAM-1 showed strong diagnostic performance and improvement over the use of CA-125 alone in detecting recurrent ovarian cancer. The experimental results warrant further clinical validation to determine their role in the early detection of recurrent ovarian cancer. (Source: Clinical Proteomics)
Source: Clinical Proteomics - June 25, 2023 Category: Biochemistry Source Type: research
Identification of ORM1, vWF, SPARC, and PPBP as immune-related proteins involved in immune thrombocytopenia by quantitative LC-MS/MS
ConclusionBioinformatics analysis identified differentially expressed proteins in bone marrow that are involved in the TNF- α signalling pathway and are related to the activation of immune function in ITP patients. These findings could provide new ideas for research on the loss of immune tolerance in ITP patients. (Source: Clinical Proteomics)
Source: Clinical Proteomics - June 24, 2023 Category: Biochemistry Source Type: research
Orthogonal proteomics methods warrant the development of Duchenne muscular dystrophy biomarkers
ConclusionsThese results demonstrate that orthogonal assays can be used to assess the analytical reliability of biomarker quantification assays, providing a means to facilitate the translation of biomarkers to clinical practice. This strategy also warrants the development of the most relevant biomarkers, markers that can be reliably quantified with different proteomics methods. (Source: Clinical Proteomics)
Source: Clinical Proteomics - June 12, 2023 Category: Biochemistry Source Type: research
Tailored therapeutic decision of rheumatoid arthritis using proteomic strategies: how to start and when to stop?
AbstractUnpredictable treatment responses have been an obstacle for the successful management of rheumatoid arthritis. Although numerous serum proteins have been proposed, there is a lack of integrative survey to compare their relevance in predicting treatment outcomes in rheumatoid arthritis. Also, little is known about their applications in various treatment stages, such as dose modification, drug switching or withdrawal. Here we present an in-depth exploration of the potential usefulness of serum proteins in clinical decision-making and unveil the spectrum of immunopathology underlying responders to different drugs. Pat...
Source: Clinical Proteomics - June 10, 2023 Category: Biochemistry Source Type: research
Comparative proteomic analysis of glomerular proteins in IgA nephropathy and IgA vasculitis with nephritis
ConclusionsThe present results suggest shared molecular mechanisms for glomerular injury in IgAN and IgAVN, except for enhanced glomerular complement activation in IgAN. Differences in the protein abundance of podocyte-associated and GBM proteins between IgAN and IgAVN patients with and without NS may be associated with the severity of proteinuria. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 13, 2023 Category: Biochemistry Source Type: research
The rapid detection of procalcitonin in septic serum using immunoaffinity MALDI chips
ConclusionsProcalcitonin from human serum was successfully enriched and detected using immunoaffinity MALDI chips. The intact PCT was characterized in 13 septic patients. The method is more specific compared to non-MS-based immunoaffinity techniques and allows observation of different variants of PCT in septic patients. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 11, 2023 Category: Biochemistry Source Type: research
Proteomic signature associated with chronic kidney disease (CKD) progression identified by data-independent acquisition mass spectrometry
ConclusionsThe in-depth proteomic characterisation of this large-scale CKD cohort is a step toward generating mechanism-based hypotheses that might lend themselves to future drug targeting. Candidate biomarkers will be validated in samples from selected patients in other large non-dialysis CKD cohorts using a targeted mass spectrometric analysis. (Source: Clinical Proteomics)
Source: Clinical Proteomics - April 20, 2023 Category: Biochemistry Source Type: research
Correction to: Phospho-heavy-labeled-spiketide FAIMS stepped-CV DDA (pHASED) provides real-time phosphoproteomics data to aid in cancer drug selection
(Source: Clinical Proteomics)
Source: Clinical Proteomics - April 8, 2023 Category: Biochemistry Source Type: research
Mitochondria and cytochrome components released into the plasma of severe COVID-19 and ICU acute respiratory distress syndrome patients
DiscussionThe results from PCR, LC –ESI–MS/MS of tryptic peptides, and cytochrome ECL assays confirmed that mitochondrial components were present in the plasma, in agreement with the established central role of the mitochondria in SARS-COV-2 biology. The cytochrome activity assay showed that there was the equivalent of at least n anogram amounts of cytochrome(s) in the plasma sample that should be clearly detectable by LC–ESI–MS/MS. The release of the luminol oxidase activity from cells into plasma forms the basis of a simple and rapid test for the severity of cell damage and lung injury in COVID-19 infection and I...
Source: Clinical Proteomics - April 8, 2023 Category: Biochemistry Source Type: research
Quantitative proteomics analysis in small cell carcinoma of cervix reveals novel therapeutic targets
ConclusionsUsing quantitative proteomics analysis, we first reported the whole-protein expression profiles in SCCC. Significant alterations were found in proteins associated with the enhancement of DNA replication and cellular motility. Besides the association with mitosis, a unique metabolic feature was detected in cases with tumor recurrence. These findings provided novel targets for disease surveillance and treatments, which warranted further validation in the future. (Source: Clinical Proteomics)
Source: Clinical Proteomics - April 8, 2023 Category: Biochemistry Source Type: research
Label-free quantitative proteomics reveals aberrant expression levels of LRG, C9, FN, A1AT and AGP1 in the plasma of patients with colorectal cancer
ConclusionsThis study identified and quantified distinct plasma proteome profiles of CRC patients. Selected CRC biomarker candidates including FN, LRG, C9, A1AT, and AGP1 may be further applied for screening larger cohorts including disease groups from other types of cancer or other diseases. (Source: Clinical Proteomics)
Source: Clinical Proteomics - April 6, 2023 Category: Biochemistry Source Type: research
An optimized workflow for MS-based quantitative proteomics of challenging clinical bronchoalveolar lavage fluid (BALF) samples
ConclusionsOverall, our described workflow consistently provided high quality proteins and tryptic peptides for MS analysis. It should enable researchers to apply MS-based proteomics to a wide-variety of studies focused on BALF clinical specimens. (Source: Clinical Proteomics)
Source: Clinical Proteomics - April 2, 2023 Category: Biochemistry Source Type: research
Proteomic characteristics and diagnostic potential of exhaled breath particles in patients with COVID-19
ConclusionOur results demonstrate the promising potential for the use of EBP fingerprints in biomarker discovery and for diagnosing pulmonary diseases, rapidly and non-invasively with minimal patient discomfort. (Source: Clinical Proteomics)
Source: Clinical Proteomics - March 27, 2023 Category: Biochemistry Source Type: research
Discovery of novel glioma serum biomarkers by proximity extension assay
ConclusionPEA is a powerful new proteomic technology for biomarker discovery. GFAP and a handful of other plasma biomarkers may be useful for early glioma detection and probably, prognosis.StatementDetecting gliomas as early as possible is highly desirable since it can significantly improve the chances of effective treatments. Reliable glioma biomarkers can timely inform glioma patients about the efficacy of their prescribed treatment. Our results reveal some novel putative glioma markers that may prove valuable, when used alone or in combination, towards improved clinical care of gliomas. In order to better appreciate the...
Source: Clinical Proteomics - March 24, 2023 Category: Biochemistry Source Type: research
