Serum proteomics of severe fever with thrombocytopenia syndrome patients
ConclusionsThrombocytopenia and multiple organ failure are the major immediate causes of death among SFTS patients. In this study, serum proteomic changes related to thrombocytopenia, abnormal immune response, and inflammatory activation were documented in SFTS patients. These findings provide useful information for understanding the clinical manifestations of SFTS. (Source: Clinical Proteomics)
Source: Clinical Proteomics - August 13, 2022 Category: Biochemistry Source Type: research
Integration of protein context improves protein-based COVID-19 patient stratification
ConclusionOur results show that the integration of protein context including protein complexes, stoichiometric ratios, pathways, network degrees, and proteins improves phenotype prediction. (Source: Clinical Proteomics)
Source: Clinical Proteomics - August 11, 2022 Category: Biochemistry Source Type: research
Proteomic and phosphoproteomic measurements enhance ability to predict ex vivo drug response in AML
In conclusion, this pilot study demonstrates strong promise for proteomics-based patient stratification to assess drug sensitivity in AML. (Source: Clinical Proteomics)
Source: Clinical Proteomics - July 27, 2022 Category: Biochemistry Source Type: research
Correction to: A novel non ‑invasive method allowing for discovery of pathologically relevant proteins from small airways
(Source: Clinical Proteomics)
Source: Clinical Proteomics - July 18, 2022 Category: Biochemistry Source Type: research
Proteomic analysis of low- and high-grade human colon adenocarcinoma tissues and tissue-derived primary cell lines reveals unique biological functions of tumours and new protein biomarker candidates
ConclusionsThe differences exhibited between tissues and cell lines highlight the additional information that can be obtained from patient-derived primary cell lines. DNA sequencing and proteomics confirmed that these cell lines can be considered suitable in vitro models of the parent tumours. Various potential biomarkers for colon adenocarcinoma initiation and progression and drug targets were identified and discussed, including seven novel markers: ACSL4, ANK2, AMER3, EXOSC1, EXOSC6, GCLM, and TFRC. (Source: Clinical Proteomics)
Source: Clinical Proteomics - July 16, 2022 Category: Biochemistry Source Type: research
Proteomics-based diagnostic peptide discovery for severe fever with thrombocytopenia syndrome virus in patients
ConclusionsProteomic analysis of SFTS patient samples revealed that nucleocapsid (N) protein is the major antigen proteins in sera of SFTS patients and N-terminal tryptic peptide of the N protein might be a useful proteomic target for direct detection of SFTS virus. These findings suggest that proteomic analysis could be an alternative tool for detection of pathogens in clinical samples and diagnosis of infectious diseases. (Source: Clinical Proteomics)
Source: Clinical Proteomics - July 16, 2022 Category: Biochemistry Source Type: research
Comparative proteomic analysis of glomerular proteins in primary and bucillamine-induced membranous nephropathy
ConclusionsBetween the pMN and BCL-induced sMN groups, we observed common and different alterations in protein levels such as known disease-associated proteins and potential disease marker proteins. (Source: Clinical Proteomics)
Source: Clinical Proteomics - July 14, 2022 Category: Biochemistry Source Type: research
Circulating proteins as predictive and prognostic biomarkers in breast cancer
AbstractBreast cancer (BC) is the most common cancer and among the leading causes of cancer death in women. It is a heterogeneous group of tumours with numerous morphological and molecular subtypes, making predictions of disease evolution and patient outcomes difficult. Therefore, biomarkers are needed to help clinicians choose the best treatment for each patient. For the last years, studies have increasingly focused on biomarkers obtainable by liquid biopsy. Circulating proteins (from serum or plasma) can be used for inexpensive and minimally invasive determination of disease risk, early diagnosis, treatment adjusting, pr...
Source: Clinical Proteomics - July 11, 2022 Category: Biochemistry Source Type: research
Optimized data-independent acquisition approach for proteomic analysis at single-cell level
ConclusionsOur results demonstrate that the optimized DIA pipeline can serve as a reliable quantitative tool for single-cell as well as sub-nano-gram proteomic analysis. (Source: Clinical Proteomics)
Source: Clinical Proteomics - July 9, 2022 Category: Biochemistry Source Type: research
Quantitative proteomics reveals protein dysregulation during T cell activation in multiple sclerosis patients compared to healthy controls
ConclusionsOur study highlights the importance of CD4+ T cell activation for MS, as proteins that change in abundance upon T cell activation are enriched for proteins encoded by MS susceptibility genes. The results provide evidence for proteomic disturbances in T cell activation in MS, and pinpoint to dysregulation of the Nur77 pathway, a biological pathway known to limit aberrant effector T cell responses. (Source: Clinical Proteomics)
Source: Clinical Proteomics - July 5, 2022 Category: Biochemistry Source Type: research
Many kinds of oxidized proteins are present more in the urine of the elderly
ConclusionsOur study is the first to show that oxidative proteins occur in urine and that oxidations are higher in older than younger ages. Perhaps improving the degree of excretion of oxidative protein in vivo through the kidney is helpful for maintaining the homeostasis of the body ’s internal environment, delaying aging and the occurrence of senile diseases. (Source: Clinical Proteomics)
Source: Clinical Proteomics - June 22, 2022 Category: Biochemistry Source Type: research
Label-free quantitative proteomic analysis of serum exosomes from patients of renal anemia: The Good and the Bad of Roxadustat
ConclusionsTFRC and HSPA8 could be an important target of the action of roxadustat, and roxadustat may increase cardiovascular risk through its influence on platelet activation. Our results provide a theoretical basis for its wider clinical application and preventing expected side effects. (Source: Clinical Proteomics)
Source: Clinical Proteomics - June 11, 2022 Category: Biochemistry Source Type: research
A novel non-invasive method allowing for discovery of pathologically relevant proteins from small airways
ConclusionThe applied analytical method was reproducible and allowed identification of pathologically interesting proteins in PEx samples from asthmatic subjects with high LCI. The results suggest that PEx based proteomics is a novel and promising approach to study respiratory diseases with small airway involvement. (Source: Clinical Proteomics)
Source: Clinical Proteomics - June 6, 2022 Category: Biochemistry Source Type: research
Proteomic alteration of endometrial tissues during secretion in polycystic ovary syndrome may affect endometrial receptivity
AbstractEmbryo implantation is a complex developmental process that requires coordinated interactions among the embryo, endometrium, and the microenvironment of endometrium factors. Even though the impaired endometrial receptivity of patients with polycystic ovary syndrome (PCOS) is known, understanding of endometrial receptivity is limited. A proteomics study in three patients with PCOS and 3 fertile women was performed to understand the impaired endometrial receptivity in patients with PCOS during luteal phases. Through isobaric tags for relative and absolute quantitation (iTRAQ) analyses, we identified 232 unique protei...
Source: Clinical Proteomics - May 28, 2022 Category: Biochemistry Source Type: research
Serum proteomics unveil characteristic protein diagnostic biomarkers and signaling pathways in patients with esophageal squamous cell carcinoma
ConclusionsOur findings propose a potential serum biomarker panel for the early detection and diagnosis of ESCC, which could potentially broaden insights into the characteristics of ESCC from the proteomic perspective. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 24, 2022 Category: Biochemistry Source Type: research
