Proteomic analysis identifies highly expressed plasma membrane proteins for detection and therapeutic targeting of specific breast cancer subtypes
AbstractIn recent years, there has been an emphasis on personalizing breast cancer treatment in order to avoid the debilitating side effects caused by broad-spectrum chemotherapeutic drug treatment. Development of personalized medicine requires the identification of proteins that are expressed by individual tumors. Herein, we reveal the identity of plasma membrane proteins that are overexpressed in estrogen receptor α-positive, HER2-positive, and triple negative breast cancer cells. The proteins we identified are involved in maintaining protein structure, intracellular homeostasis, and cellular architecture; enhancing cel...
Source: Clinical Proteomics - September 19, 2018 Category: Biochemistry Source Type: research
Phosphotyrosine profiling of human cerebrospinal fluid
ConclusionsThe findings from this deep phosphotyrosine profiling of CSF samples have the potential to identify novel disease-related phosphotyrosine-containing peptides in CSF. (Source: Clinical Proteomics)
Source: Clinical Proteomics - September 12, 2018 Category: Biochemistry Source Type: research
Antibiotic treatment modulates protein components of cytotoxic outer membrane vesicles of multidrug-resistant clinical strain, Acinetobacter baumannii DU202
ConclusionIn vitro stress such as antibiotic treatment can modulate proteome components inA. baumannii OMVs and thereby influence pathogenicity. (Source: Clinical Proteomics)
Source: Clinical Proteomics - August 31, 2018 Category: Biochemistry Source Type: research
iTRAQ-based analysis of sperm proteome from normozoospermic men achieving the rescue-ICSI pregnancy after the IVF failure
ConclusionsThe sealtered sperm proteins and the increased spontaneous acrosome reaction rate might account for this unexplained male infertility in the R-ICSI pregnancy patients. The present proteomic results will throw light on the better understanding of the unexplained infertile mechanisms underlying these normozoospermic man who achieved R-ICSI pregnancy after IVF failure. (Source: Clinical Proteomics)
Source: Clinical Proteomics - August 21, 2018 Category: Biochemistry Source Type: research
Proteomic distinction of renal oncocytomas and chromophobe renal cell carcinomas
ConclusionWe present one of the first differential proteome profiling studies on ROs and chRCCs and highlight differential abundance of LAMP1 and ITGAV in these renal tumors. (Source: Clinical Proteomics)
Source: Clinical Proteomics - August 3, 2018 Category: Biochemistry Source Type: research
Residual tissue repositories as a resource for population-based cancer proteomic studies
ConclusionsThese studies demonstrate that residual FFPE tissue specimens, of varying age and collection site, are a promising source of protein for proteomic investigations if paired with rigorously verified mass spectrometry workflows. (Source: Clinical Proteomics)
Source: Clinical Proteomics - August 3, 2018 Category: Biochemistry Source Type: research
Proteomic distinction of renal oncocytomas and chromophobe renal cell carcinomas
ConclusionWe present one of the first differential proteome profiling studies on ROs and chRCCs and highlight differential abundance of LAMP1 and ITGAV in these renal tumors. (Source: Clinical Proteomics)
Source: Clinical Proteomics - August 3, 2018 Category: Biochemistry Source Type: research
Residual tissue repositories as a resource for population-based cancer proteomic studies
ConclusionsThese studies demonstrate that residual FFPE tissue specimens, of varying age and collection site, are a promising source of protein for proteomic investigations if paired with rigorously verified mass spectrometry workflows. (Source: Clinical Proteomics)
Source: Clinical Proteomics - August 3, 2018 Category: Biochemistry Source Type: research
Comparison of iTRAQ and SWATH in a clinical study with multiple time points
ConclusionsOverall, we conclude that SWATH should be preferred for biomarker discovery studies when analyzing limited volumes of clinical samples collected at multiple time points.Trial RegisterationThe study was approved by the Ethics Committee at Tampere University Hospital and was registered in EU clinical trials register (EudraCT Number: 2010-021039-14). (Source: Clinical Proteomics)
Source: Clinical Proteomics - July 30, 2018 Category: Biochemistry Source Type: research
MALDI-TOF peptidomic analysis of serum and post-prostatic massage urine specimens to identify prostate cancer biomarkers
ConclusionsMALDI-TOF/MS serum peptidome profiling was more efficacious with respect to post-prostatic massage urine analysis in discriminating PCa. (Source: Clinical Proteomics)
Source: Clinical Proteomics - July 25, 2018 Category: Biochemistry Source Type: research
