Circulating microvesicles and exosomes in small cell lung cancer by quantitative proteomics
ConclusionsIn comparing SCLC patients and healthy individuals, several differentially expressed proteins were identified. This is the first study showing that circulating extracellular vesicles may encompass specific proteins with potential diagnostic attributes for SCLC, thereby opening new opportunities as novel non-invasive markers. (Source: Clinical Proteomics)
Source: Clinical Proteomics - January 7, 2022 Category: Biochemistry Source Type: research
Uncomplicated Plasmodium vivax malaria: mapping the proteome from circulating platelets
ConclusionsThe PLT proteome analyzed in this study suggests that PLTs actively respond toP. vivax infection. Altogether, our findings suggest important roles of PF4/CXCL4 during uncomplicatedP. vivax infection through a possible intracellular localization. Our study shows that platelets are active responders toP. vivax infection, inhibiting intraerythrocytic parasite development. Future studies are needed to further investigate the molecular pathways of interaction between platelet proteins found in this study and host response, which could affect parasite control as well as disease progression. (Source: Clinical Proteomics)
Source: Clinical Proteomics - January 5, 2022 Category: Biochemistry Source Type: research
Integration of transcriptome and proteome profiles in placenta accreta reveals trophoblast over-migration as the underlying pathogenesis
ConclusionA poor correlation was observed between the transcriptome and proteome data and MeCP2, PODN, and ApoD decreased in transcriptome and proteome profiling, resulting in increased migration of trophoblasts in the PA group, which clarify the mechanism of PA and might be the biomarkers or therapy targets in the future. (Source: Clinical Proteomics)
Source: Clinical Proteomics - December 29, 2021 Category: Biochemistry Source Type: research
Urine proteomics identifies biomarkers for diabetic kidney disease at different stages
ConclusionsOur study demonstrated the potential for urinary proteomics as a noninvasive method for DKD diagnosis and identifying high-risk patients for progression monitoring. (Source: Clinical Proteomics)
Source: Clinical Proteomics - December 29, 2021 Category: Biochemistry Source Type: research
Proteomics and functional study reveal kallikrein-6 enhances communicating hydrocephalus
ConclusionsCollectively, KLK6 participates in the development of CH and might provide a new target for CH treatment. (Source: Clinical Proteomics)
Source: Clinical Proteomics - December 16, 2021 Category: Biochemistry Source Type: research
OmicsOne: associate omics data with phenotypes in one-click
ConclusionsOmicsOne can effectively simplify data analysis and reveal the significant associations between phenotypes and potential biomarkers, including genes, proteins, and glycopeptides, in minutes to assist users to understand aberrant biological processes. (Source: Clinical Proteomics)
Source: Clinical Proteomics - December 11, 2021 Category: Biochemistry Source Type: research
A validated analysis pipeline for mass spectrometry-based vitreous proteomics: new insights into proliferative diabetic retinopathy
ConclusionsThis study demonstrates a feasible, rigorous, and scalable method that can be applied to future proteomic studies of vitreous and identifies previously unrecognized metabolic pathways that advance understanding of diabetic retinopathy. (Source: Clinical Proteomics)
Source: Clinical Proteomics - December 3, 2021 Category: Biochemistry Source Type: research
Combined proteomic/transcriptomic signature of recurrence post-liver transplantation for hepatocellular carcinoma beyond Milan
ConclusionsSignificantly increased LGALS3 and LGALS3BP gene and protein expression on explant were associated with post-transplant recurrence, whereas increased ALDH1A1 was associated with absence of recurrence in patients transplanted for HCC beyond Milan criteria. This combined proteomic/transcriptomic signature could help in predicting HCC recurrence risk and guide post-transplant surveillance. (Source: Clinical Proteomics)
Source: Clinical Proteomics - November 18, 2021 Category: Biochemistry Source Type: research
Analytical validation of a multi-biomarker algorithmic test for prediction of progressive kidney function decline in patients with early-stage kidney disease
ConclusionsThe set of analytical validation studies demonstrated robust analytical performance across all three biomarkers contributing to the KidneyIntelX risk score, meeting or exceeding specifications established during characterization studies. Notably, reproducibility of the composite risk score demonstrated that expected analytical laboratory variation did not impact the assigned risk category, and therefore, the clinical validity of the reported results. (Source: Clinical Proteomics)
Source: Clinical Proteomics - November 17, 2021 Category: Biochemistry Source Type: research
A SISCAPA-based approach for detection of SARS-CoV-2 viral antigens from clinical samples
In this study, we adopted a SISCAPA-based enrichment approach using anti-peptide antibodies generate d against peptides from the nucleocapsid protein of SARS-CoV-2. We developed a targeted workflow in which nasopharyngeal swab samples were digested followed by enrichment of viral peptides using the anti-peptide antibodies and targeted parallel reaction monitoring (PRM) analysis using a high-resolut ion mass spectrometer. This workflow was applied to 41 RT-PCR-confirmed clinical SARS-CoV-2 positive nasopharyngeal swab samples and 30 negative samples. The workflow employed was highly specific as none of the target peptides w...
Source: Clinical Proteomics - October 22, 2021 Category: Biochemistry Source Type: research
Quantitative proteomics reveals the effect of Yigu decoction (YGD) on protein expression in bone tissue
ConclusionsYGD can significantly increase the bone mineral density (BMD) of osteoporotic rats and may play a therapeutic role by regulating target proteins involved in multiple signaling pathways. Therefore, these results improve the understanding of the OP mechanism and provide an experimental basis for the clinical application of YGD in OP treatment. (Source: Clinical Proteomics)
Source: Clinical Proteomics - October 12, 2021 Category: Biochemistry Source Type: research
Association between cardiovascular disease- and inflammation-related serum biomarkers and poor lung function in elderly
ConclusionWe found that serum levels of PLAUR are associated with pulmonary function decline in older adults. PLAUR is activated following inflammation and promotes matrix metallopeptidase (MMP) activation and extracellular matrix (ECM) degradation. This implies that PLAUR could play a role in the early phase of COPD pathogenesis. (Source: Clinical Proteomics)
Source: Clinical Proteomics - September 28, 2021 Category: Biochemistry Source Type: research
iTRAQ-based quantitative proteomic analysis of thoracic aortas from adult rats born to preeclamptic dams
ConclusionsAlthough the results require further functional validation, these data provide novel findings related to vascular function impairment in the adult offspring of preeclamptic mothers. (Source: Clinical Proteomics)
Source: Clinical Proteomics - August 21, 2021 Category: Biochemistry Source Type: research
Validation and update of a multivariable prediction model for the identification and management of patients at risk for hepatocellular carcinoma
ConclusionsThe ASAP model is an accurate tool for HCC risk estimation that requires recalibration before use in a new region because calibration varies with clinical environments. Additionally, rational risk stratification and risk-based management decision-making, e.g., 3-month follow-up recommendations for targeted individuals, helped improve HCC surveillance, which warrants assessment in larger cohorts. (Source: Clinical Proteomics)
Source: Clinical Proteomics - August 19, 2021 Category: Biochemistry Source Type: research
Robust optimization of SWATH-MS workflow for human blood serum proteome analysis using a quality by design approach
ConclusionsUsing design of experiments permits identification of a robust operating window for SWATH-MS. The method gives a good understanding of proteomics assays and greater data-driven confidence in SWATH-MS performance. (Source: Clinical Proteomics)
Source: Clinical Proteomics - August 12, 2021 Category: Biochemistry Source Type: research
