Maternal serum CFHR4 protein as a potential non-invasive marker of ventricular septal defects in offspring: evidence from a comparative proteomics study
ConclusionsSubsequent logistic regression and receiver operating characteristic analysis suggested maternal serum CFHR4 as a promising biomarker of fetal VSD. Further studies are warranted to verify the findings. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 19, 2022 Category: Biochemistry Source Type: research
Airway fibrin formation cascade in allergic asthma exacerbation: implications for inflammation and remodeling
ConclusionsProtein abundance of the fibrin formation cascade, platelet activation and remodeling are associated with late-phase leukocyte numbers and markers of remodeling. Patients with lower FEV1 have distinct dynamic responses to allergen. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 19, 2022 Category: Biochemistry Source Type: research
13C15N: glucagon-based novel isotope dilution mass spectrometry method for measurement of glucagon metabolism in humans
ConclusionsThe methods described in this study for simultaneous detection and quantitation of glucagon tracers have clinical utility for investigating glucagon kinetics in vivo in humans. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 19, 2022 Category: Biochemistry Source Type: research
Transcriptome profiling and proteomic validation reveals targets of the androgen receptor signaling in the BT-474 breast cancer cell line
DiscussionOur findings suggest that AR modulates the metabolism of BT-474 cells by affecting the expression of a large number of genes and proteins. Based on further pathway analysis, we suggest that androgen receptor acts as a tumor suppressor in the BT-474 cells. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 14, 2022 Category: Biochemistry Source Type: research
Optimized sample preparation and data analysis for TMT proteomic analysis of cerebrospinal fluid applied to the identification of Alzheimer ’s disease biomarkers
ConclusionWe have evaluated several analytical aspects of TMT proteomics in CSF. The results of our study provide practical guidelines to improve the accuracy of quantification and aid in the design of sample preparation and analytical protocol. The AD biomarker list extracted from the EMIF-AD cohort can provide a valuable basis for future biomarker studies and help elucidate pathogenic mechanisms in AD. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 14, 2022 Category: Biochemistry Source Type: research
Label-free LC –MS/MS proteomics analyses reveal proteomic changes in oxidative stress and the SOD antioxidant strategy in TM cells
ConclusionsKey proteins and signaling pathways play important roles in the mechanisms of oxidative stress and antioxidant strategies in TM cells. ICAM-1 knockdown can suppress the apoptosis of TM cells induced by H2O2, which may reveal new therapeutic targets and biomarkers for glaucoma. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 14, 2022 Category: Biochemistry Source Type: research
Correction: A SISCAPA-based approach for detection of SARS-CoV-2 viral antigens from clinical samples
(Source: Clinical Proteomics)
Source: Clinical Proteomics - May 4, 2022 Category: Biochemistry Source Type: research
Systematic evaluation and optimization of protein extraction parameters in diagnostic FFPE specimens
ConclusionsPreanalytical protein extraction can be optimized at various levels to improve unbiased protein extraction and to reduce time and costs. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 2, 2022 Category: Biochemistry Source Type: research
Use of MS-GUIDE for identification of protein biomarkers for risk stratification of patients with prostate cancer
ConclusionUsing MS-GUIDE we identified fibronectin and vitronectin as candidate biomarkers for prostate cancer risk stratification. (Source: Clinical Proteomics)
Source: Clinical Proteomics - April 27, 2022 Category: Biochemistry Source Type: research
Moving translational mass spectrometry imaging towards transparent and reproducible data analyses: a case study of an urothelial cancer cohort analyzed in the Galaxy framework
ConclusionHere, we show that translational MSI data analysis in a fully transparent and reproducible manner is possible and we would like to encourage the community to join our efforts. (Source: Clinical Proteomics)
Source: Clinical Proteomics - April 19, 2022 Category: Biochemistry Source Type: research
Data-independent acquisition mass spectrometry in severe rheumatic heart disease (RHD) identifies a proteomic signature showing ongoing inflammation and effectively classifying RHD cases
ConclusionsThese results support the presence of an ongoing inflammatory response in RHD, at a time when severe valve disease has developed, and distant from previous episodes of acute rheumatic fever. This biomarker signature could have potential utility in recognizing different degrees of ongoing inflammation in RHD patients, which may, in turn, be related to prognostic severity. (Source: Clinical Proteomics)
Source: Clinical Proteomics - March 22, 2022 Category: Biochemistry Source Type: research
Integrative omics reveals subtle molecular perturbations following ischemic conditioning in a porcine kidney transplant model
ConclusionsOur data identifies subtle molecular phenotypes in porcine kidneys following RIC, and this knowledge could potentially aid optimization of remote ischemic conditioning protocols in renal transplantation. (Source: Clinical Proteomics)
Source: Clinical Proteomics - February 14, 2022 Category: Biochemistry Source Type: research
Quantitative proteomic analysis of serum-purified exosomes identifies putative pre-eclampsia-associated biomarkers
ConclusionsWe have identified and validated differential exosomal proteins in liquid biopsy of pregnant women that open new possibilities for early detection of pre-eclampsia. Additionally, the functional impact of the proteome composition of purified pre-eclamptic exosomes in target cells provides new information to better understand changes in embryo-maternal interactions and, consequently, the pathogenesis of this disease. (Source: Clinical Proteomics)
Source: Clinical Proteomics - February 10, 2022 Category: Biochemistry Source Type: research
Secreted protein markers in oral squamous cell carcinoma (OSCC)
ConclusionsWe identified several key proteins and processes that are associated with OSCC tissues, underscoring the importance of altered secretion. Cancer-associated OSCC secretome proteins present in saliva have potential to be used as novel non-invasive biomarkers for the diagnosis of OSCC.Graphical Abstract (Source: Clinical Proteomics)
Source: Clinical Proteomics - February 8, 2022 Category: Biochemistry Source Type: research
Identification of biomarkers of chronic kidney disease among kidney-derived proteins
ConclusionOverall, our results indicate that our approach of focusing on kidney-derived proteins is useful for identifying new CKD biomarkers and that C1q has potential as a biomarker of renal function. (Source: Clinical Proteomics)
Source: Clinical Proteomics - January 11, 2022 Category: Biochemistry Source Type: research
