Proteomics identification of radiation-induced changes of membrane proteins in the rat model of arteriovenous malformation in pursuit of targets for brain AVM molecular therapy
ConclusionThis work provides additional potential target protein molecules for evaluation in animal trials to promote intravascular thrombosis in AVM vessels post radiosurgery. (Source: Clinical Proteomics)
Source: Clinical Proteomics - December 26, 2018 Category: Biochemistry Source Type: research
Fast quantitative urinary proteomic profiling workflow for biomarker discovery in kidney cancer
ConclusionsThe result suggested the feasibility of applying the high throughput workflow in extensive urinary proteome profiling and clinical relevant biomarker discovery. (Source: Clinical Proteomics)
Source: Clinical Proteomics - December 22, 2018 Category: Biochemistry Source Type: research
The plasma peptides of ovarian cancer
ConclusionHere we show that separation of endogenous peptides with a step gradient of organic/water and differential centrifugation followed by random and independent sampling by LC –ESI–MS/MS with analysis of peptide frequency and intensity by SQL Server and R revealed significant difference in the ex vivo cleavage of peptides between ovarian cancer and other clinical treatments. There was striking agreement between the proteins discovered from cancer plasma versus previo us biomarkers discovered in tumors by genetic or biochemical methods. The results indicate that variation in plasma proteins from ovarian cancer ma...
Source: Clinical Proteomics - December 21, 2018 Category: Biochemistry Source Type: research
Proteomic analysis of serum proteins from HIV/AIDS patients with Talaromyces marneffei infection by TMT labeling-based quantitative proteomics
ConclusionsThis is the first report to provide a global proteomic profile of serum samples from HIV/TM-coinfected patients. Our data provide insights into the proteins that are involved as host response factors during infection. These data shed new light on the molecular mechanisms that are dysregulated and contribute to the pathogenesis of HIV/TM coinfection. IL1RL1 and THBS1 are promising diagnostic markers for HIV/TM-coinfected patients although further large-scale studies are needed. Thus, quantitative proteomic analysis revealed molecular differences between the HIV/TM-coinfected and HIV-mono-infected individuals, and...
Source: Clinical Proteomics - December 21, 2018 Category: Biochemistry Source Type: research
A two-step strategy for identification of plasma protein biomarkers for endometrial and ovarian cancer
ConclusionThe PEA assays represent useful tools for identification of new biomarkers for gynecologic cancers. The selected protein assays could be used to distinguish benign tumors from ovarian and endometrial cancer in women diagnosed with an unknown suspicious pelvic mass. The panels could also be used in population screening, for identification of women in need of specialized gynecologic transvaginal ultrasound examination.FundingThe Swedish Cancer Foundation, Vinnova (SWELIFE), The Foundation for Strategic Research (SSF), Assar Gabrielsson Foundation. (Source: Clinical Proteomics)
Source: Clinical Proteomics - December 1, 2018 Category: Biochemistry Source Type: research
The plasma peptidome
ConclusionsThe random and independent sampling of pre-fractionated blood peptides by LC-ESI-MS/MS with SQL Server-R analysis revealed the largest plasma proteome to date and was a practical method to quantify and compare the frequency or log10 intensity of individual proteins cleaved ex vivo across populations of plasma samples from multiple clinical locations to discover treatment-specific variation using classical statistics suitable for clinical science. It was possible to identify and quantify nearly all human proteins from EDTA plasma and compare the results of thousands of LC –ESI–MS/MS experiments from multiple ...
Source: Clinical Proteomics - December 1, 2018 Category: Biochemistry Source Type: research
Correction to: Quantitative phosphoproteomic analysis reveals reciprocal activation of receptor tyrosine kinases between cancer epithelial cells and stromal fibroblasts
Unfortunately, after publication of this article [1], errors were noticed in Figs. 3 and 4. (Source: Clinical Proteomics)
Source: Clinical Proteomics - November 17, 2018 Category: Biochemistry Source Type: research
Differential analysis of quantitative proteome and acetyl-proteome profiling between premenopausal and postmenopausal ovarian tissues
ConclusionGlobal and acetylated proteome Profiles in ovary differ between the premenopausal and postmenopausal groups. These proteomic-level changes may offer some potential biological markers to identify the pathological changes in ovary and help relieve and treat the associated signs and symptoms, and ultimately improve women ’s health care. (Source: Clinical Proteomics)
Source: Clinical Proteomics - November 16, 2018 Category: Biochemistry Source Type: research
Using matrix assisted laser desorption ionisation mass spectrometry (MALDI-MS) profiling in order to predict clinical outcomes of patients with heart failure
ConclusionsThe results obtained in this study suggest that a cluster of plasma peptides using MALDI-MS can reliably predict clinical outcomes in HF that may help enable precision medicine in HF. (Source: Clinical Proteomics)
Source: Clinical Proteomics - November 2, 2018 Category: Biochemistry Source Type: research
Proteomic profiling of liver tissue from the mdx - 4cv mouse model of Duchenne muscular dystrophy
ConclusionsThe proteomic results presented here indicate that the intricate and multifaceted pathogenesis of themdx-4cv model of dystrophinopathy is associated with secondary alterations in the liver affecting especially fatty acid transportation. Since FABP5 levels were also shown to be elevated in serum from dystrophic mice, this protein might be a useful indicator for monitoring liver changes in X-linked muscular dystrophy. (Source: Clinical Proteomics)
Source: Clinical Proteomics - October 29, 2018 Category: Biochemistry Source Type: research
Quality assessment and interference detection in targeted mass spectrometry data using machine learning
AbstractAdvances in the field of targeted proteomics and mass spectrometry have significantly improved assay sensitivity and multiplexing capacity. The high-throughput nature of targeted proteomics experiments has increased the rate of data production, which requires development of novel analytical tools to keep up with data processing demand. Currently, development and validation of targeted mass spectrometry assays require manual inspection of chromatographic peaks from large datasets to ensure quality, a process that is time consuming, prone to inter- and intra-operator variability and limits the efficiency of data inte...
Source: Clinical Proteomics - October 6, 2018 Category: Biochemistry Source Type: research
Investigation of post-translational modifications in type 2 diabetes
AbstractThe investigation of post-translational modifications (PTMs) plays an important role for the study of type 2 diabetes. The importance of PTMs has been realized with the advancement of analytical techniques. The challenging detection and analysis of post-translational modifications is eased by different enrichment methods and by high throughput mass spectrometry based proteomics studies. This technology along with different quantitation methods provide accurate knowledge about the changes happening in disease conditions as well as in normal conditions. In this review, we have discussed PTMs such as phosphorylation, ...
Source: Clinical Proteomics - September 24, 2018 Category: Biochemistry Source Type: research
Plasma lipoproteome in Alzheimer ’s disease: a proof-of-concept study
The objective of this study is to provide initial proof-of-concept that the plasma lipoproteome more likely differ between AD cases and controls when measured in individual plasma lipoprotein fractions than when measured as total in immunodepleted plasma.MethodsWe first developed a targeted proteomics method based on selected reaction monitoring (SRM) and liquid chromatography and tandem mass spectrometry for measurement of 120 tryptic peptides from 79 proteins that are commonly present in plasma lipoproteins. Then in a proof-of concept case –control study of 5 AD cases and 5 sex- and age-matched controls, we applied the...
Source: Clinical Proteomics - September 20, 2018 Category: Biochemistry Source Type: research
