Proteomics analysis of colon cancer progression
ConclusionNevertheless, the study has provided significant insights into CC stage progression for further investigation. (Source: Clinical Proteomics)
Source: Clinical Proteomics - December 27, 2019 Category: Biochemistry Source Type: research
The plasma peptides of breast versus ovarian cancer
ConclusionThere was striking agreement between the breast cancer plasma peptides and proteins discovered by LC–ESI–MS/MS with previous biomarkers from tumors, cells lines or body fluids by genetic or biochemical methods. The results indicate that variation in plasma peptides from breast cancer versus ovarian cancer may be directly discovered by LC–ESI–M S/MS that will be a powerful tool for clinical research. It may be possible to use a battery of sensitive and robust linear quadrupole ion traps for random and independent sampling of plasma from a multisite clinical trial. (Source: Clinical Proteomics)
Source: Clinical Proteomics - December 22, 2019 Category: Biochemistry Source Type: research
Quantitative proteome analysis of Merkel cell carcinoma cell lines using SILAC
ConclusionsThe results of this study present a new protein map of MCC and provide deeper insights in the biology of MCC. Data are available via ProteomeXchange with identifier PXD008181. (Source: Clinical Proteomics)
Source: Clinical Proteomics - December 18, 2019 Category: Biochemistry Source Type: research
Clinical method evaluation of hemoglobin S and C identification by top-down selected reaction monitoring and electron transfer dissociation
ConclusionsThis top-down SRM ETD method can be used in a clinical environment to detect Hb S and Hb C. (Source: Clinical Proteomics)
Source: Clinical Proteomics - December 16, 2019 Category: Biochemistry Source Type: research
The associations of self-rated health with cardiovascular risk proteins: a proteomics approach
ConclusionsPoor SRH was associated with raised concentrations of many plasma proteins. However, the relationships were largely attenuated by adjustments for CVD risk factors, comorbidity and psycho-social factors. Leptin and CCL20 were associated with poor SRH in the present study and could potentially be involved in the SRH –CVD link. (Source: Clinical Proteomics)
Source: Clinical Proteomics - November 17, 2019 Category: Biochemistry Source Type: research
Quantitative analysis of Mycobacterium avium subsp . hominissuis proteome in response to antibiotics and during exposure to different environmental conditions
AbstractMycobacterium avium subsp.hominissuis (MAH) belongs to the clinically important non-tuberculous mycobacterial group that infects immunocompromised patients and individuals with underling lung conditions. The need for prolonged therapy is a major challenge of MAH treatment, influencing the development of persistent and drug-resistant infections. The reason why bactericidal drugs take several months to eliminate MAH is unknown. To investigate MAH proteome remodeling under aerobic, anaerobic and biofilm conditions (as it is encountered in patient lungs) and identify metabolic changes potentially associated with bacter...
Source: Clinical Proteomics - November 12, 2019 Category: Biochemistry Source Type: research
iTRAQ-based quantitative proteomic analysis provides insight for molecular mechanism of neuroticism
ConclusionsOur study revealed the characteristics of the neurotic personality proteome, which might be intrinsic mechanism of the neurotic population. (Source: Clinical Proteomics)
Source: Clinical Proteomics - November 7, 2019 Category: Biochemistry Source Type: research
Matrisome analysis of intrahepatic cholangiocarcinoma unveils a peculiar cancer-associated extracellular matrix structure
ConclusionsOverall, this study contributes to the understanding of molecular basis underlying desmoplasia in iCCA and indicates the type III collagen as a promising therapeutic target. (Source: Clinical Proteomics)
Source: Clinical Proteomics - October 29, 2019 Category: Biochemistry Source Type: research
iTRAQ and PRM-based quantitative proteomics in early recurrent spontaneous abortion: biomarkers discovery
ConclusionsCD45, PSG1, and Prdx-2, were significantly dysregulated in the samples of ERSA and could become important biomarkers for the prediction and diagnosis of ERSA. Larger ‑scale studies are required to confirm the diagnostic value of these biomarkers. (Source: Clinical Proteomics)
Source: Clinical Proteomics - October 17, 2019 Category: Biochemistry Source Type: research
N -GlycositeAtlas: a database resource for mass spectrometry-based human N-linked glycoprotein and glycosylation site mapping
ConclusionsThe entire humanN-glycoproteome database as well as 22 sub-databases associated with individual tissues or body fluids can be downloaded from theN-GlycositeAtlas website athttp://nglycositeatlas.biomarkercenter.org. (Source: Clinical Proteomics)
Source: Clinical Proteomics - September 6, 2019 Category: Biochemistry Source Type: research
Potential early clinical stage colorectal cancer diagnosis using a proteomics blood test panel
ConclusionMS-based proteomics in combination with ultradepletion strategies have an immense potential of identifying diagnostic protein biosignature. (Source: Clinical Proteomics)
Source: Clinical Proteomics - August 27, 2019 Category: Biochemistry Source Type: research
iTRAQ plasma proteomics analysis for candidate biomarkers of type 2 incipient diabetic nephropathy
ConclusionsThese findings offer a theoretical basis for the early treatment of diabetic nephropathy. (Source: Clinical Proteomics)
Source: Clinical Proteomics - July 30, 2019 Category: Biochemistry Source Type: research
Screening differentially expressed proteins from co-cultured hematopoietic cells and bone marrow-derived stromal cells by quantitative proteomics (SILAC) method
ConclusionAltogether, we recommend such quantitative proteomics approach for the studies of the hematopoietic –stroma cross-talk, differentially expressed proteins and related signaling pathways identification. The differentially expressed proteins identified from this current SILAC method will provide a useful basis for ongoing studies of crosstalk between stromal cells and hematopoietic cells in co-cult ure systems. All these result suggested our ongoing studies can focus on the mechanisms underlying CKAP4 increase and CD44 decrease in co-cultured hematopoietic cells, and the increase of LCP1 and decrease of CAPG in co...
Source: Clinical Proteomics - July 17, 2019 Category: Biochemistry Source Type: research
Predictive proteomic signatures for response of pancreatic cancer patients receiving chemotherapy
ConclusionBased on the cohort investigated, our data suggested that systemic proteome alterations involved in pathways associated with inflammation, immunoresponse, coagulation and complement cascades may be reporters of chemo-treatment outcome in PDAC patients. For the majority of the patients involved, the panel consisting of PZ, SHBG, VWF and CA 19-9 was able to segregate Good-responders from Limited-responders effectively. Our data also showed that dramatic fluctuations of biomarker concentration in the circulating system of a PDAC patient, which might result from biological heterogeneity or confounding complications, ...
Source: Clinical Proteomics - July 16, 2019 Category: Biochemistry Source Type: research
Correction to: A proteome-wide immuno-mass spectrometric identification of serum autoantibodies
In the original version of the article [1], an error was noticed under the heading “Immunoprecipitation on protein G magnetic beads” in “Methods” section. (Source: Clinical Proteomics)
Source: Clinical Proteomics - July 16, 2019 Category: Biochemistry Source Type: research
