Screening differentially expressed proteins from co-cultured hematopoietic cells and bone marrow-derived stromal cells by quantitative proteomics (SILAC) method

ConclusionAltogether, we recommend such quantitative proteomics approach for the studies of the hematopoietic –stroma cross-talk, differentially expressed proteins and related signaling pathways identification. The differentially expressed proteins identified from this current SILAC method will provide a useful basis for ongoing studies of crosstalk between stromal cells and hematopoietic cells in co-cult ure systems. All these result suggested our ongoing studies can focus on the mechanisms underlying CKAP4 increase and CD44 decrease in co-cultured hematopoietic cells, and the increase of LCP1 and decrease of CAPG in co-cultured stromal cell. The proteomic profiles from the KG1a/stromal cell co-cult ure system give new molecular insights into the roles of these cells in MDS pathophysiology and related bone disease.

Source: Clinical Proteomics - July 17, 2019 Category: Biochemistry Source Type: research