Lipid metabolism impairment in patients with sepsis secondary to hospital acquired pneumonia, a proteomic analysis
ConclusionProteomic analysis pointed to impairment of lipid metabolism as a major change in septic patients secondary to HAP, which was further validated by the reduced levels of cholesterol moieties and apolipoproteins in plasma. Our results stress the involvement of lipids in the pathogenesis of sepsis, which is in accordance with previous reports supporting the role of lipid moieties in pathogen toxin clearance and in modulating inflammatory responses. (Source: Clinical Proteomics)
Source: Clinical Proteomics - July 15, 2019 Category: Biochemistry Source Type: research
Discovering endometriosis biomarkers with multiplex cytokine arrays
ConclusionsThese findings show the utility and reliability of multiplex arrays in deciphering new biomarker panels for disease detection and may offer clues for understanding this mysterious disease. (Source: Clinical Proteomics)
Source: Clinical Proteomics - July 10, 2019 Category: Biochemistry Source Type: research
Correction to: Comprehensive proteome and phosphoproteome profiling shows negligible influence of RNAlater on protein abundance and phosphorylation
In the version of this article that was originally published [1], some information in the “Acknowledgements” section was omitted. (Source: Clinical Proteomics)
Source: Clinical Proteomics - July 10, 2019 Category: Biochemistry Source Type: research
Phenotyping multiple subsets in Sj ögren’s syndrome: a salivary proteomic SWATH-MS approach towards precision medicine
ConclusionsConstellations of proteins, including S100A proteins, characterize different pSS subsets reflecting either salivary gland dysfunction or inflammation. Salivary proteomics may foster future research projects ultimately aimed at developing personalized treatments for pSS patients. (Source: Clinical Proteomics)
Source: Clinical Proteomics - June 19, 2019 Category: Biochemistry Source Type: research
A proteome-wide immuno-mass spectrometric identification of serum autoantibodies
ConclusionsThis study demonstrates the effectiveness of a proteomic approach to identify serum autoantibody targets, using immunoaffinity purification followed by tandem mass spectrometry. Our methodology is applicable for proteome-wide analysis of autoantibody targets in a wide variety of clinical settings. (Source: Clinical Proteomics)
Source: Clinical Proteomics - June 19, 2019 Category: Biochemistry Source Type: research
Differential motility parameters and identification of proteomic profiles of human sperm cryopreserved with cryostraw and cryovial
ConclusionThe proteomic profiles of human sperm are greatly affected by cryopreservation with either type of CPC. GO analysis revealed that most of the differentially identified sperm proteins enriched in the extracellular membrane-bounded organelles, cytoplasm and cytosol. In addition, 106 susceptible proteins having known identities related to sperm functions were identified. In general, cryovial seems to be the preferred CPC for human sperm cryopreservation based on the post-thaw motility parameters and the effect on sperm proteomic profiles. These results are beneficial for the insight into the understanding of the cry...
Source: Clinical Proteomics - June 18, 2019 Category: Biochemistry Source Type: research
Elucidating the endogenous synovial fluid proteome and peptidome of inflammatory arthritis using label-free mass spectrometry
ConclusionsThe discovery-phase data generated herein has provided a basis for the identification of candidates with the greatest potential to serve as novel serum biomarkers specific to inflammatory arthritides. Moreover, these findings facilitate the understanding of possible disease mechanisms specific to each subtype. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 29, 2019 Category: Biochemistry Source Type: research
Plasma proteomic and autoantibody profiles reveal the proteomic characteristics involved in longevity families in Bama, China
ConclusionsProteomic and autoantibody profiles varied between the offspring of longevous and normal families which are from the same area and shared the same environmental factors. The identified differences were reported to be involved in several physiological and pathological pathways. The identified proteins will contribute to a better understanding of the proteomic characteristics of people from Bama longevous area and a revelation of the molecular mechanisms of longevity. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 19, 2019 Category: Biochemistry Source Type: research
Biomarkers of blood cadmium and incidence of cardiovascular events in non-smokers: results from a population-based proteomics study
ConclusionsB-Cd in non-smokers was associated with eight potential plasma biomarkers of CVD and kidney injury. The results suggest pathways for the associations between B-Cd and CVD and kidney injury. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 14, 2019 Category: Biochemistry Source Type: research
Stage-associated differences in the serum N - and O -glycan profiles of patients with non-small cell lung cancer
ConclusionsThe serum glycan profiles were significantly different between NSCLC and healthy control, and most of the glycosylation changes had occurred at early stage. Further evaluation is needed to examine the diagnostic value of the glycan markers identified in this study. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 9, 2019 Category: Biochemistry Source Type: research
Quantitative proteomic analyses of CD4 + and CD8 + T cells reveal differentially expressed proteins in multiple sclerosis patients and healthy controls
ConclusionOur study provides evidence for proteomic differences in T cells from relapsing –remitting MS patients compared to healthy controls and also identifies dysregulation of proteins encoded from MS susceptibility genes. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 7, 2019 Category: Biochemistry Source Type: research
Comprehensive proteome and phosphoproteome profiling shows negligible influence of RNAlater on protein abundance and phosphorylation
In this study we carried out a systematic and comprehensive analysis of the effect of RNAlater on the proteome and phosphoproteome using high-resolution mass spectrometry. PDAC tissues from three patients were individually pulverized and the tissue powders of each patient were divided into two portions, one of which was incubated in RNAlater at 4 °C for 24 h (RNAlater tissue) while the other was kept at – 80 °C (frozen tissue). Comprehensive quantitative profiling experiments on the RNAlater tissues and the frozen tissues resulted in the identification of 99,136 distinct peptides of 8803 protein groups and 17,345 ...
Source: Clinical Proteomics - April 24, 2019 Category: Biochemistry Source Type: research
Variability of serum novel serum peptide biomarkers correlates with the disease states of multiple myeloma
ConclusionsThe potential value of the four peptides in monitoring MM treatment response was arised from their close correlation with MM disease states. (Source: Clinical Proteomics)
Source: Clinical Proteomics - April 22, 2019 Category: Biochemistry Source Type: research
Deep undepleted human serum proteome profiling toward biomarker discovery for Alzheimer ’s disease
ConclusionsOur results show an ultra-deep serum discovery study by TMT –LC/LC–MS/MS, and a validation experiment by TOMAHAQ targeted LC–MS3. The MS-based discovery and validation methods are of general use for biomarker discovery from complex biofluids (e.g. serum proteome). This pilot study also identified deregulated proteins, in particular proteins associated with mitochondrial function in the AD serum samples. These proteins may serve as novel AD candidate biomarkers. (Source: Clinical Proteomics)
Source: Clinical Proteomics - April 16, 2019 Category: Biochemistry Source Type: research
Quantitative proteomic analysis of prostate tissue specimens identifies deregulated protein complexes in primary prostate cancer
ConclusionsIn summary, to the best of our knowledge, the study represents the first large-scale and quantitative, albeit indirect, comparison of individual protein complexes in human PCa tissue specimens. It may serve as a useful resource for better understanding the deregulation of protein complexes in primary PCa. (Source: Clinical Proteomics)
Source: Clinical Proteomics - April 12, 2019 Category: Biochemistry Source Type: research
