Effects of cryopreservation and long-term culture on biological characteristics and proteomic profiles of human umbilical cord-derived mesenchymal stem cells
ConclusionThis suggests that protein expression may be used as an indicator of hUC-MSCs security testing before applying in clinical settings, and it is also expected to provide the foundation or standardization guide of hUC-MSCs applications in regenerative medicine. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 23, 2020 Category: Biochemistry Source Type: research
Recent advances in mass spectrometry based clinical proteomics: applications to cancer research
AbstractCancer biomarkers have transformed current practices in the oncology clinic. Continued discovery and validation are crucial for improving early diagnosis, risk stratification, and monitoring patient response to treatment. Profiling of the tumour genome and transcriptome are now established tools for the discovery of novel biomarkers, but alterations in proteome expression are more likely to reflect changes in tumour pathophysiology. In the past, clinical diagnostics have strongly relied on antibody-based detection strategies, but these methods carry certain limitations. Mass spectrometry (MS) is a powerful method t...
Source: Clinical Proteomics - May 23, 2020 Category: Biochemistry Source Type: research
Mass spectrometry-based microbiological testing for blood stream infection
ConclusionMALDI-TOF MS is now an essential tool for rapid bacterial ID of pathogens that cause blood stream infection. For the purpose of assessment of susceptibility and resistance to antibiotics of the pathogens, the roles of liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS) will increase in the future. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 12, 2020 Category: Biochemistry Source Type: research
Development of a novel method for the quantification of tyrosine 39 phosphorylated α- and β-synuclein in human cerebrospinal fluid
ConclusionsWe anticipate that this optimized two-step enrichment-based PRM detection method will help monitor c-Abl activation in PD patients and can also be used to quantify other phosphotyrosine peptides of low abundance in biological samples. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 3, 2020 Category: Biochemistry Source Type: research
A comprehensive analysis of sialolith proteins and the clinical implications
ConclusionSialolith formation presents similarities with the hyperoxaluria that forms kidney stones, which suggests the possibility of a common origin. Further verification of a common origin could fundamentally change the way in which lithiasis is studied and treated. (Source: Clinical Proteomics)
Source: Clinical Proteomics - March 30, 2020 Category: Biochemistry Source Type: research
Early detection of Ebola virus proteins in peripheral blood mononuclear cells from infected mice
ConclusionsWhile mass spectrometry is not currently amenable to field diagnostics, these results suggest that viral protein enrichment in PBMCs in tandem with highly sensitive immunoassays platforms, could lead to the development of a rapid, high-throughput diagnostic platform for pre-symptomatic detection of EBOV infection. (Source: Clinical Proteomics)
Source: Clinical Proteomics - March 16, 2020 Category: Biochemistry Source Type: research
Urinary proteomics of Henoch-Sch önlein purpura nephritis in children using liquid chromatography-tandem mass spectrometry
ConclusionsOur study provides new insights into the assessment of HSPN progression in children, as well as new potential biomarkers. The data confirm the value of the urinary proteome in capturing the emergence of HSPN. (Source: Clinical Proteomics)
Source: Clinical Proteomics - March 11, 2020 Category: Biochemistry Source Type: research
The impact of ultraviolet- and infrared-based laser microdissection technology on phosphoprotein detection in the laser microdissection-reverse phase protein array workflow
AbstractReversible protein phosphorylation represents a key mechanism by which signals are transduced in eukaryotic cells. Dysregulated phosphorylation is also a hallmark of carcinogenesis and represents key drug targets in the precision medicine space. Thus, methods that preserve phosphoprotein integrity in the context of clinical tissue analyses are crucially important in cancer research. Here we investigated the impact of UV laser microdissection (UV LMD) and IR laser capture microdissection (IR LCM) on phosphoprotein abundance of key cancer signaling protein targets assessed by reverse-phase protein microarray (RPPA). ...
Source: Clinical Proteomics - March 8, 2020 Category: Biochemistry Source Type: research
The different expression of glycogen phosphorylases in renal clear cell renal carcinoma and chromophobe renal carcinoma
ConclusionWe found different expression of glycogen phosphorylases in CCRCC and CHRCC by quantitative proteomics, which provides potential therapeutic targets in the future. (Source: Clinical Proteomics)
Source: Clinical Proteomics - February 25, 2020 Category: Biochemistry Source Type: research
Correction to: Proteomic analysis of degradation ubiquitin signaling by ubiquitin occupancy changes responding to 26S proteasome inhibition
In the original publication of the article, Figure 2d was published incorrectly. The corrected Fig. 2 was given in this Correction article [1]. The original article has been corrected. (Source: Clinical Proteomics)
Source: Clinical Proteomics - February 20, 2020 Category: Biochemistry Source Type: research
Discovery of a putative blood-based protein signature associated with response to ALK tyrosine kinase inhibition
ConclusionIn conjunction with ALK mutation, the expression of this proteomic signature may represent a liquid biopsy-based marker of long-term response to crizotinib in NSCLC. Expanding the utility of prognostic biomarkers of response duration could influence choice of therapy, therapeutic sequencing, and potentially the need for alternative or combination therapy.Trial registration ClinicalTrials.gov, NCT02041468. Registered 22 January 2014,https://clinicaltrials.gov/ct2/show/NCT02041468?term=NCT02041468&rank=1 (Source: Clinical Proteomics)
Source: Clinical Proteomics - February 6, 2020 Category: Biochemistry Source Type: research
Prediction of neoadjuvant chemotherapeutic efficacy in patients with locally advanced gastric cancer by serum IgG glycomics profiling
ConclusionWe here firstly present the profiling of IgGN-glycans in pretreatment serum of LAGC. The alterations in IgGN-glycome may be personalized biomarkers to predict the response to NACT in LAGC and help to illustrate the relationship between immunity and effect of NACT. (Source: Clinical Proteomics)
Source: Clinical Proteomics - February 5, 2020 Category: Biochemistry Source Type: research
Quantitative site- and structure-specific N-glycoproteomics characterization of differential N-glycosylation in MCF-7/ADR cancer stem cells
ConclusionsQuantitative site- and structure-specific N-glycoproteomics characterization may help illustrate the cell stemness property. (Source: Clinical Proteomics)
Source: Clinical Proteomics - February 4, 2020 Category: Biochemistry Source Type: research
Proteomic analysis of degradation ubiquitin signaling by ubiquitin occupancy changes responding to 26S proteasome inhibition
ConclusionsIn summary, the computational method, previously described for glycosylation analysis, was used in this study for the assessment of ubiquitin stoichiometries and applied directly to proteomic data to distinguish degradation from non-degradation ubiquitin functions. (Source: Clinical Proteomics)
Source: Clinical Proteomics - January 24, 2020 Category: Biochemistry Source Type: research
The omics revolution: beyond genomics. A meeting report
AbstractThe “omics revolution: beyond genomics” satellite meeting, run under the auspices of the Australian Peptide Association, The Human Proteome Organisation (HUPO) and the HUPO Australia/New Zealand Chromosome 7 initiative, was held at the Oaks Resort, Port Douglas, Queensland, Australia, on 8th Septemb er 2019, immediately prior to the 13th Australian Peptide Conference. The meeting, which had over 100 participants representing Australasia, Europe and America, focused on recent advances in omics-related technologies, including mass spectrometry, biosensors and CryoEM, which will assist in the clin ical translation...
Source: Clinical Proteomics - January 23, 2020 Category: Biochemistry Source Type: research
