Discovery of non-invasive biomarkers for the diagnosis of endometriosis
ConclusionsWe have identified menstrual cycle- and endometriosis-associated protein changes linked to various cellular processes that are potential biomarkers and that provide insight into the biology of endometriosis. Our data indicate that the markers tested, whilst not useful alone, have improved diagnostic accuracy when used in combination and demonstrate menstrual cycle specificity. Tissue heterogeneity and blood contamination is likely to have hindered biomarker discovery, whilst a small sample size precludes accurate determination of performance by cycle phase. Independent validation of these biomarker panels in a l...
Source: Clinical Proteomics - April 5, 2019 Category: Biochemistry Source Type: research
Development of a glycoproteomic strategy to detect more aggressive prostate cancer using lectin-immunoassays for serum fucosylated PSA
ConclusionWe have developed quantitative lectin-immunoassays for serum fucosylated PSA. Our data demonstrated that fucosylated PSA –AAL, % fucosylated PSA–AAL and fucosylated PSA–LCA levels could be effective biomarkers to differentiate aggressive prostate cancer [especially Gleason 7 (4 + 3) or above] from non-aggressive disease. We believe that application of these lectin-immunoassays to a larger patient population is needed to evaluate the clinical utilities of fucosylated PSA using AAL–PSA and LCA–PSA for aggressive prostate cancer. (Source: Clinical Proteomics)
Source: Clinical Proteomics - April 5, 2019 Category: Biochemistry Source Type: research
Identification of prothymosin alpha (PTMA) as a biomarker for esophageal squamous cell carcinoma (ESCC) by label-free quantitative proteomics and Quantitative Dot Blot (QDB)
ConclusionIn this study, label-free quantitative proteomics combined with QDB revealed that PTMA expression was up-regulated in ESCC tissues, and PTMA might be a potential candidate for ESCC. Since Western Blot cannot achieve rapid and high-throughput screening of mass spectrometry results, the emergence of QDB meets this demand and provides an effective method for the identification of biomarkers. (Source: Clinical Proteomics)
Source: Clinical Proteomics - April 4, 2019 Category: Biochemistry Source Type: research
Age-associated changes in human tear proteome
ConclusionsOverall, the results show the common age-dependent alterations in tear fluid protein profile, which demonstrate similar age-associated alterations of biological functions previously shown in other tissue and sample types. (Source: Clinical Proteomics)
Source: Clinical Proteomics - March 29, 2019 Category: Biochemistry Source Type: research
A multiplex immunoassay of serum biomarkers for the detection of uveal melanoma
ConclusionsThe multiplex immunoassay provides sufficient analytical performance to evaluate serum biomarkers that complement each other in detection of UM, and warrants further validation with a larger number of patient samples. (Source: Clinical Proteomics)
Source: Clinical Proteomics - March 4, 2019 Category: Biochemistry Source Type: research
Affinity depletion versus relative protein enrichment: a side-by-side comparison of two major strategies for increasing human cerebrospinal fluid proteome coverage
AbstractCerebrospinal fluid (CSF) is in direct contact with the central nervous system. This makes human CSF an attractive source of potential biomarkers for neurologic diseases. Similarly to blood plasma, proteomic analysis of CSF is complicated by a high dynamic range of individual protein concentrations and by the presence of several highly abundant proteins. To deal with the abundant human CSF proteins, methods developed for blood plasma/serum are routinely used. Multiple affinity removal systems and protein enrichment of less abundant proteins using a combinatorial peptide ligand library are among the most frequent ap...
Source: Clinical Proteomics - February 26, 2019 Category: Biochemistry Source Type: research
Rapid detection of colistin resistance protein MCR-1 by LC –MS/MS
ConclusionsThis work demonstrates the feasibility of rapid detection of the MCR-1 protein in bacterial isolates by LC –MS/MS. (Source: Clinical Proteomics)
Source: Clinical Proteomics - February 26, 2019 Category: Biochemistry Source Type: research
Characterization of the plasma proteome of nonhuman primates during Ebola virus disease or melioidosis: a host response comparison
ConclusionsThese results advance our understanding of the global plasma proteomic response to EBOV andBp infection in relevant primate models for human disease and provide insight into potential innate immune response differences between viral and bacterial infections. (Source: Clinical Proteomics)
Source: Clinical Proteomics - February 7, 2019 Category: Biochemistry Source Type: research
Quantitative proteomic characterization of lung tissue in idiopathic pulmonary fibrosis
ConclusionsThis study provides a list of proteomes that were detected in IPF lung tissue by iTRAQ technology combined with LC –MS/MS. The findings of this study will contribute better understanding to the pathogenesis of IPF and facilitate the development of therapeutic targets. (Source: Clinical Proteomics)
Source: Clinical Proteomics - February 6, 2019 Category: Biochemistry Source Type: research
Proteomic analysis of plasma exosomes to differentiate malignant from benign pulmonary nodules
ConclusionsThis study provides a new perspective on the identification of novel detection strategies for benign and malignant PNs. We hope our findings can provide clues for the identification of benign and malignant PNs. (Source: Clinical Proteomics)
Source: Clinical Proteomics - February 2, 2019 Category: Biochemistry Source Type: research
Ulcerative colitis: functional analysis of the in-depth proteome
ConclusionsThis explorative study describes the most affected functions in UC tissue. Our results complemented previous findings substantially. Decreased abundances of signal peptidase complex proteins in UC are a new discovery. (Source: Clinical Proteomics)
Source: Clinical Proteomics - January 29, 2019 Category: Biochemistry Source Type: research
Combination of laser microdissection, 2D-DIGE and MALDI-TOF MS to identify protein biomarkers to predict colorectal cancer spread
AbstractBiomarkers are urgently required to support current histological staging to provide additional accuracy in stratifying colorectal cancer (CRC) patients according to risk of spread to properly assign adjuvant chemotherapy after surgery. Chemotherapy is given to patients with stage III to reduce the risk of recurrence but is controversial in stage II patients. Up to 25% of stage II patients will relapse within 5 years after tumor removal and when this occurs cure is seldom possible. The aim of this study was to identify protein biomarkers to stratify risk of spread of CRC patients. Laser micro-dissection was used t...
Source: Clinical Proteomics - January 22, 2019 Category: Biochemistry Source Type: research
Validation of a novel model for the early detection of hepatocellular carcinoma
ConclusionsCompared to each biomarker alone, the combination of AFP and PIVKA-II with age and gender improved the accuracy of detecting HCC and differentiating HCC from non-malignant liver disease. (Source: Clinical Proteomics)
Source: Clinical Proteomics - January 16, 2019 Category: Biochemistry Source Type: research
High accuracy differentiating autoimmune pancreatitis from pancreatic ductal adenocarcinoma by immunoglobulin G glycosylation
ConclusionsAIP and PDAC patients have distinct IgG-glycosylation profilings. IgG-glycosylation could different PDAC from AIP with high accuracy. (Source: Clinical Proteomics)
Source: Clinical Proteomics - January 3, 2019 Category: Biochemistry Source Type: research
Identification of Cytauxzoon felis antigens via protein microarray and assessment of expression library immunization against cytauxzoonosis
ConclusionsProtein microarray facilitated high-throughput identification of novel antigens, substantially increasing the pool of characterizedC. felis antigens. These antigens should be considered for development ofC. felis vaccines, diagnostics, and therapeutics. (Source: Clinical Proteomics)
Source: Clinical Proteomics - December 29, 2018 Category: Biochemistry Source Type: research
