Evaluation of the potential of Pap test fluid and cervical swabs to serve as clinical diagnostic biospecimens for the detection of ovarian cancer by mass spectrometry-based proteomics
ConclusionsOur results suggest that Pap test fixatives and cervical swabs are a rich source of tumor-specific biomarkers for ovarian cancer, which could be developed as a test for ovarian cancer detection. (Source: Clinical Proteomics)
Source: Clinical Proteomics - January 7, 2021 Category: Biochemistry Source Type: research
Global proteome profiling of human livers upon ischemia/reperfusion treatment
This study therefore may provide a research direction for the design of new therapeutic strategies for hepatic ischemia/reperfusion injury. (Source: Clinical Proteomics)
Source: Clinical Proteomics - January 6, 2021 Category: Biochemistry Source Type: research
Identification of differentially expressed proteins and clinicopathological significance of HMGB2 in cervical cancer
AbstractTo investigate the complexity of proteomics in cervical cancer tissues, we used isobaric tags for relative and absolute quantitation (iTRAQ)-based mass spectrometry analysis on a panel of normal cervical tissues (N), high-grade squamous intraepithelial lesion tissues (HSIL) and cervical cancer tissues (CC). Total 72 differentially expressed proteins were identified both in CC vs N and CC vs HSIL. The expression of HMGB2 was markedly higher in CC than that in HSIL and N. High HMGB2 expression was significantly correlated with primary tumor size, invasion and tumor stage. The up-regulated HMGB2 was discovered to be a...
Source: Clinical Proteomics - January 6, 2021 Category: Biochemistry Source Type: research
Label-free proteomic analysis of serum exosomes from paroxysmal atrial fibrillation patients
ConclusionsThese results revealed the composition and potential function of AF serum exosomes, thus providing a new perspective on the complement system and protein folding to AF. (Source: Clinical Proteomics)
Source: Clinical Proteomics - January 6, 2021 Category: Biochemistry Source Type: research
Tear proteomic profile in three distinct ocular surface diseases: keratoconus, pterygium, and dry eye related to graft-versus-host disease
ConclusionsWe demonstrated herein that mass spectrometry-based proteomics was able to indicate proteins that differentiate three distinct ocular conditions, which is a promising tool for the diagnosis of ocular diseases. (Source: Clinical Proteomics)
Source: Clinical Proteomics - December 7, 2020 Category: Biochemistry Source Type: research
Clinical proteomics for prostate cancer: understanding prostate cancer pathology and protein biomarkers for improved disease management
AbstractFollowing the introduction of routine Prostate Specific Antigen (PSA) screening in the early 1990 ′s, Prostate Cancer (PCa) is often detected at an early stage. There are also a growing number of treatment options available and so the associated mortality rate is generally low. However, PCa is an extremely complex and heterogenous disease and many patients suffer disease recurrence following i nitial therapy. Disease recurrence commonly results in metastasis and metastatic PCa has an average survival rate of just 3–5 years. A significant problem in the clinical management of PCa is being able to differentiate ...
Source: Clinical Proteomics - November 20, 2020 Category: Biochemistry Source Type: research
Comparative analysis of differentially abundant proteins quantified by LC –MS/MS between flash frozen and laser microdissected OCT-embedded breast tumor samples
ConclusionsThe proteomic profiles of the OCT/LMD-TS samples were more similar to those from OCT/LMD-T samples than FF/HOM samples, suggesting a strong influence from the sample processing methods. These results indicate that in LC –MS/MS proteomic studies, FF/HOM samples exhibit different protein expression profiles from OCT/LMD samples and thus, results from these two different methods cannot be directly compared. (Source: Clinical Proteomics)
Source: Clinical Proteomics - November 7, 2020 Category: Biochemistry Source Type: research
Subclinical effects of remote ischaemic conditioning in human kidney transplants revealed by quantitative proteomics
ConclusionsTogether, our results indicate sub-clinical systemic and organ-localised effects of RIC. (Source: Clinical Proteomics)
Source: Clinical Proteomics - November 2, 2020 Category: Biochemistry Source Type: research
Application of SWATH mass spectrometry in the identification of circulating proteins does not predict future weight gain in early psychosis
AbstractWeight gain is a common consequence of treatment with antipsychotic drugs in early psychosis, leading to further morbidity and poor treatment adherence. Identifying tools that can predict weight change in early psychosis may contribute to better-individualised treatment and adherence. Recently we showed that proteomic profiling with sequential window acquisition of all theoretical fragment ion spectra (SWATH) mass spectrometry (MS) can identify individuals with pre-diabetes more likely to experience weight change in relation to lifestyle change. We investigated whether baseline proteomic profiles predicted weight c...
Source: Clinical Proteomics - October 27, 2020 Category: Biochemistry Source Type: research
A robust multiplex immunoaffinity mass spectrometry assay (PromarkerD) for clinical prediction of diabetic kidney disease
ConclusionsAn immunoaffinity capture targeted mass spectrometry assay was developed and optimised. It showed statistically comparable results to those obtained from the original immunodepletion method and was also able to provide comparable results when deployed to an independent laboratory. Taking a research grade assay and optimising to a clinical grade workflow provides insights into the future of multiplex biomarker measurement with an immunoaffinity mass spectrometry foundation. In the current format the PromarkerD immunoaffinity assay has the potential to make a significant impact on prediction of diabetic kidney dis...
Source: Clinical Proteomics - October 20, 2020 Category: Biochemistry Source Type: research
Early changes in tear film protein profiles after femtosecond LASIK surgery
ConclusionsThe present study showed that an uneventful femtosecond LASIK refractive surgery induced a significant immune cell migration and inflammation-associated changes in tear proteomics profile quickly after the operation, but the expression of most proteins recovered almost completely to the preoperative levels within the first month. The individual proteins identified in our study are potential targets for the follow-up and modification of LASIK-induced biochemical processes. (Source: Clinical Proteomics)
Source: Clinical Proteomics - October 19, 2020 Category: Biochemistry Source Type: research
A proteomic repertoire of autoantigens identified from the classic autoantibody clinical test substrate HEp-2 cells
ConclusionsThis study provides a proteomic repertoire of confirmed and potential autoantigens for future studies, and the findings are consistent with a mechanism for autoantigenicity: how self-molecules may form molecular complexes with DS to elicit autoimmunity. Our data contribute to the molecular etiology of autoimmunity and may deepen our understanding of autoimmune diseases. (Source: Clinical Proteomics)
Source: Clinical Proteomics - September 20, 2020 Category: Biochemistry Source Type: research
A nomogram based on glycomic biomarkers in serum and clinicopathological characteristics for evaluating the risk of peritoneal metastasis in gastric cancer
ConclusionsThe nomogram achieved an individualized assessment of the risk of PM in GC patients; thus, the nomogram could be used to assist clinical decision-making before surgery. (Source: Clinical Proteomics)
Source: Clinical Proteomics - September 18, 2020 Category: Biochemistry Source Type: research
Development of robust targeted proteomics assays for cerebrospinal fluid biomarkers in multiple sclerosis
ConclusionsWe present 37 high-quality PRM assays across 21 CSF-proteins found to be affected by multiple sclerosis, along with a recommended workflow for future development of new assays. The assays can directly be used by others, thus enabling better comparison between studies. Finally, the assays can robustly and stably monitor biological processes in multiple sclerosis patients over time, thus potentially aiding in diagnosis and prognosis, and ultimately in treatment decisions. (Source: Clinical Proteomics)
Source: Clinical Proteomics - September 17, 2020 Category: Biochemistry Source Type: research
Chloride intracellular channel 4 participate in the protective effect of Ginkgolide B in MPP+ injured MN9D cells: insight from proteomic analysis
ConclusionsQuantitative comparative proteomic analysis identified differentially expressed proteins associated with GB and GB derivatives. We further verified the expression of CLIC4 by western blotting and immunocytochemistry assay. This bio-information on the identified pathways and differentially expressed proteins such as CLIC4 provide more targeted directions for the synthesis of more effective and targeted GB derivatives for the treatment of neurological disorders. (Source: Clinical Proteomics)
Source: Clinical Proteomics - September 4, 2020 Category: Biochemistry Source Type: research
