Development of an ID-LC –MS/MS method using targeted proteomics for quantifying cardiac troponin I in human serum
ConclusionsThe analytical method demonstrated high analytical performance in accurately quantifying cardiac troponin I levels in human serum. The proposed analytical method has the potential to facilitate the harmonization of cTnI results between clinical laboratories, assign target values to secondary certified reference materials and support reliable measurement of cTnI.Graphical Abstract (Source: Clinical Proteomics)
Source: Clinical Proteomics - September 27, 2023 Category: Biochemistry Source Type: research
Identification of potential molecular targets for the treatment of cluster 1 human pheochromocytoma and paraganglioma via comprehensive proteomic characterization
ConclusionsApplication of a combined proteomic strategy recently presented as “Pitchfork” enabled quantitative analysis of both, membrane and non-membrane proteome, and resulted in identification of 10 potential drug targets in human PPGL. Seven membrane proteins localized on the cell surface and three non-membrane druggable enzymes proteins were identified and verified a s significantly upregulated in PPGL. All the proteins have been previously shown to be upregulated in several human cancers, and play direct role in cancer progression. Marked upregulation of these proteins along with their localization and establishe...
Source: Clinical Proteomics - September 25, 2023 Category: Biochemistry Source Type: research
Systematic review of type 1 diabetes biomarkers reveals regulation in circulating proteins related to complement, lipid metabolism, and immune response
ConclusionsBiomarkers analyzed in this systematic review highlight alterations in specific biological processes in T1D, including complement, lipid metabolism, and immune response pathways, and may have potential for further use in the clinic as prognostic or diagnostic assays. (Source: Clinical Proteomics)
Source: Clinical Proteomics - September 21, 2023 Category: Biochemistry Source Type: research
Identification and verification of plasma protein biomarkers that accurately identify an ectopic pregnancy
ConclusionsOverall, four multivariable logistic models were identified that had significantly better prediction of having EP than those logistic models with single biomarkers. Model 4 (NOTUM, PAEP, PAPPA, ADAM12) had the highest AUC (0.987) and accuracy (96%). However, because the models are statistically similar, all markers in the four models and other highly correlated markers should be considered in further validation studies. (Source: Clinical Proteomics)
Source: Clinical Proteomics - September 15, 2023 Category: Biochemistry Source Type: research
Proteome analysis of CD5-positive diffuse large B cell lymphoma FFPE tissue reveals downregulation of DDX3X, DNAJB1, and B cell receptor signaling pathway proteins including BTK and Immunoglobulins
ConclusionsIn conclusion, DNAJB1 and DDX3X, BTK, and a set of immunoglobulins are promising biomarkers. Probably, the suppression of BCR signaling is the unique phenotype of CD5-positive DLBCL. This formalin-fixed paraffin-embedded (FFPE)-based profiling may help to develop novel therapeutic molecularly targeted drugs for treating DLBCL. (Source: Clinical Proteomics)
Source: Clinical Proteomics - September 13, 2023 Category: Biochemistry Source Type: research
New mechanisms and biomarkers of lymph node metastasis in cervical cancer: reflections from plasma proteomics
ConclusionProteomic signatures developed in this study reflected the potential plasma diagnostic markers and new possible pathogenesis mechanisms in the LVSI and LNM of cervical cancer. (Source: Clinical Proteomics)
Source: Clinical Proteomics - September 9, 2023 Category: Biochemistry Source Type: research
Correction to: Proteomic analysis of human synovial fluid reveals potential diagnostic biomarkers for ankylosing spondylitis
(Source: Clinical Proteomics)
Source: Clinical Proteomics - September 2, 2023 Category: Biochemistry Source Type: research
Cerebrospinal fluid camk2a levels at baseline predict long-term progression in multiple sclerosis
ConclusionTargeted approaches to identify and quantify biomarkers associated with MS in the CSF may inform on long term MS outcomes. CAMK2A may be one of several candidates, warranting further exploration. (Source: Clinical Proteomics)
Source: Clinical Proteomics - August 29, 2023 Category: Biochemistry Source Type: research
Mass spectrometry-based proteomics as an emerging tool in clinical laboratories
AbstractMass spectrometry (MS)-based proteomics have been increasingly implemented in various disciplines of laboratory medicine to identify and quantify biomolecules in a variety of biological specimens. MS-based proteomics is continuously expanding and widely applied in biomarker discovery for early detection, prognosis and markers for treatment response prediction and monitoring. Furthermore, making these advanced tests more accessible and affordable will have the greatest healthcare benefit.This review article highlights the new paradigms MS-based clinical proteomics has created in microbiology laboratories, cancer res...
Source: Clinical Proteomics - August 26, 2023 Category: Biochemistry Source Type: research
Proteomic analysis of 92 circulating proteins and their effects in cardiometabolic diseases
ConclusionThrough large-scale analysis of protein quantitative trait loci, we provide a comprehensive overview of common variants associated with plasma proteins. We highlight possible biological relationships which may serve as a basis for further investigation into possible causal roles in cardiometabolic diseases. (Source: Clinical Proteomics)
Source: Clinical Proteomics - August 7, 2023 Category: Biochemistry Source Type: research
Identification of SARS-CoV-2 biomarkers in saliva by transcriptomic and proteomics analysis
AbstractThe detection of SARS-CoV-2 biomarkers by real time PCR (rRT-PCR) has shown that the sensitivity of the test is negatively affected by low viral loads and the severity of the disease. This limitation can be overcome by the use of more sensitive approaches such as mass spectrometry (MS), which has not been explored for the detection of SARS-CoV-2 proteins in saliva. Thus, this study aimed at assessing the translational applicability of mass spectrometry-based proteomics approaches to identify viral proteins in saliva from people diagnosed with COVID-19 within fourteen days after the initial diagnosis, and to compare...
Source: Clinical Proteomics - August 3, 2023 Category: Biochemistry Source Type: research
Proteomic analysis identifies subgroups of patients with active systemic lupus erythematosus
ConclusionsUnsupervised proteomic analysis identifies clusters of patients with active SLE, that are associated with clinical and serological features, which may facilitate biomarker discovery. The observed proteomic heterogeneity further supports the need for a personalised approach to treatment in SLE. (Source: Clinical Proteomics)
Source: Clinical Proteomics - July 29, 2023 Category: Biochemistry Source Type: research
Comparative proteomics analysis in different stages of urothelial bladder cancer for identification of potential biomarkers: highlighted role for antioxidant activity
ConclusionWe identified several alterations in protein expression involved in canonical pathways which were correlated with the clinical outcomes suggested might be useful as promising biomarkers for early detection, monitoring, and prognosis of UBC. (Source: Clinical Proteomics)
Source: Clinical Proteomics - July 27, 2023 Category: Biochemistry Source Type: research
Associations of circulating proteins with lipoprotein profiles: proteomic analyses from the OmniHeart randomized trial and the Atherosclerosis Risk in Communities (ARIC) Study
ConclusionsWe identified proteins that mediate the association between healthy dietary patterns varying in macronutrients and lipoproteins in a randomized feeding study and an observational study.Trial registrationNCT00051350 at clinicaltrials.gov. (Source: Clinical Proteomics)
Source: Clinical Proteomics - July 3, 2023 Category: Biochemistry Source Type: research
Development of a rapid and specific MALDI-TOF mass spectrometric assay for SARS-CoV-2 detection
AbstractWe have developed a rapid and highly specific assay for detecting and monitoring SARS-CoV-2 infections by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). As MALDI-TOF mass spectrometers are available in a clinical setting, our assay has the potential to serve as alternative to the commonly used reverse transcriptase quantitative polymerase chain reaction (RT-qPCR). Sample preparation prior to MALDI-TOF-MS involves the tryptic digestion of SARS-CoV-2 proteins, followed by an enrichment of virus-specific peptides from SARS-CoV-2 nucleoprotein via magnetic antibody beads. O...
Source: Clinical Proteomics - July 1, 2023 Category: Biochemistry Source Type: research
