Proteomic analysis of sialoliths from calcified, lipid and mixed groups as a source of potential biomarkers of deposit formation in the salivary glands
AbstractSalivary stones, also known as sialoliths, are formed in a pathological situation in the salivary glands. So far, neither the mechanism of their formation nor the factors predisposing to their formation are known despite several hypotheses. While they do not directly threaten human life, they significantly deteriorate the patient ’s quality of life. Although this is not a typical research material, attempts are made to apply various analytical tools to characterise sialoliths and search for the biomarkers in their proteomes. In this work, we used mass spectrometry and SWATH-MS qualitative and quantitative analysi...
Source: Clinical Proteomics - March 22, 2023 Category: Biochemistry Source Type: research
Quantitative proteomics identifies and validates urinary biomarkers of rhabdomyosarcoma in children
ConclusionsThese findings provide novel biomarkers in urine that are easy to translate into clinical diagnosis of RMS and illustrate the value of global and targeted urine proteomics to identify and qualify candidate biomarkers for noninvasive molecular diagnosis. (Source: Clinical Proteomics)
Source: Clinical Proteomics - March 14, 2023 Category: Biochemistry Source Type: research
Potential biomarker proteins for aspiration pneumonia detected by shotgun proteomics using buccal mucosa samples: a cross-sectional case –control study
ConclusionOur findings suggest that oral salivary proteins, which are obtained non-invasively, can be utilized for the detection of AP. (Source: Clinical Proteomics)
Source: Clinical Proteomics - March 9, 2023 Category: Biochemistry Source Type: research
Proteomic analysis identifies dysregulated proteins and associated molecular pathways in a cohort of gallbladder cancer patients of African ancestry
ConclusionThe identified dysregulated proteins help in understanding GBC molecular mechanisms in our patient group. Furthermore, the alteration of specific proteins in both tissue and plasma samples suggests their potential utility as biomarkers of GBC in this sample cohort. (Source: Clinical Proteomics)
Source: Clinical Proteomics - March 1, 2023 Category: Biochemistry Source Type: research
Changes of serum IgG glycosylation patterns in rheumatoid arthritis
ConclusionLectin microarray is an effective and reliable technique for analyzing multiple lectin –glycan interactions. RA, RA-seropositive, and RA-ILD patients exhibit distinct glycan profiles, respectively. Altered levels of glycosylation may be related to the pathogenesis of the disease, which could provide a direction for new biomarkers identification. (Source: Clinical Proteomics)
Source: Clinical Proteomics - February 21, 2023 Category: Biochemistry Source Type: research
Integrating the serum proteomic and fecal metaproteomic to analyze the impacts of overweight/obesity on IBD: a pilot investigation
ConclusionThis study provided high-quality multi-omics data of IBD serum and fecal samples, which enabled deciphering the molecular bases of clinical phenotypes of IBD, revealing the impacts of microbiota on IBD, and emphasizing the important role of overweight/obesity in IBD. (Source: Clinical Proteomics)
Source: Clinical Proteomics - February 9, 2023 Category: Biochemistry Source Type: research
Are the results from a multiplex proteomic assay and a conventional immunoassay for NT-proBNP and GDF-15 comparable?
ConclusionExcept for the highest levels of NT-proBNP, we suggest that PEA data for NT-proBNP and GDF-15 reliably reflects absolute plasma levels and contains similar prognostic information. (Source: Clinical Proteomics)
Source: Clinical Proteomics - January 24, 2023 Category: Biochemistry Source Type: research
The novel biomarkers for assessing clinical benefits of continuous renal replacement therapy in pediatric sepsis: a pilot study
ConclusionElevated serum LYZ and LRG1 levels are associated with clinical benefits of CRRT during sepsis. (Source: Clinical Proteomics)
Source: Clinical Proteomics - January 18, 2023 Category: Biochemistry Source Type: research
Combination of automated sample preparation and micro-flow LC –MS for high-throughput plasma proteomics
ConclusionsThis workflow is suitable for the analysis of large-scale clinical plasma samples with its simple and time-saving operation, and the results demonstrate the feasibility of discovering significantly changed plasma proteins and distinguishing different patient groups. (Source: Clinical Proteomics)
Source: Clinical Proteomics - January 7, 2023 Category: Biochemistry Source Type: research
Investigation on the mechanisms of human sperm DNA damage based on the proteomics analysis by SWATH-MS
ConclusionsProteomic markers of sperm with high DNA fragmentation can be identified by the SWATH-MS and bioinformatic analysis, and new protein markers and posttranslational modifications related to sperm DNA damage are expected to be intensively explored. Our findings may improve our understanding of the basic molecular mechanism of sperm DNA damage. (Source: Clinical Proteomics)
Source: Clinical Proteomics - January 6, 2023 Category: Biochemistry Source Type: research
A proteomic profile of the healthy human placenta
ConclusionThese results suggest that normal proteomic variations exist within the anatomical structure of the placenta, thus recommending serial sectioning methodology for consistent placental research. (Source: Clinical Proteomics)
Source: Clinical Proteomics - January 2, 2023 Category: Biochemistry Source Type: research
LC-MS/MS based metabolomics and proteomics reveal candidate biomarkers and molecular mechanism of early IgA nephropathy
ConclusionIn this study, we combined proteomics and metabolomics techniques to analyze the plasma of IgAN patients and healthy individuals, constructing a biomarker panel, which could provide new insights and provide potential novel molecular diagnoses for IgAN. (Source: Clinical Proteomics)
Source: Clinical Proteomics - December 27, 2022 Category: Biochemistry Source Type: research
Novel plasma protein biomarkers from critically ill sepsis patients
ConclusionsTargeted proteomics with feature selection identified proteins altered in critically ill sepsis patients relative to healthy control subjects. Correlations between protein expression and clinical/laboratory variables were identified, each providing pathophysiological insight. Our exploratory data provide a rationale for further hypothesis-driven sepsis research. (Source: Clinical Proteomics)
Source: Clinical Proteomics - December 27, 2022 Category: Biochemistry Source Type: research
Alterations in the pH of pancreatic juice are associated with chymotrypsin C inactivation and lithostathine precipitation in chronic pancreatitis patients: a proteomic approach
ConclusionOur results suggest that chymotrypsin C (CTRC) is degraded in an acidic environment, leading to the precipitation of lithostathine in the ductal lumen.Graphical Abstract (Source: Clinical Proteomics)
Source: Clinical Proteomics - December 26, 2022 Category: Biochemistry Source Type: research
Phospho-heavy-labeled-spiketide FAIMS stepped-CV DDA (pHASED) provides real-time phosphoproteomics data to aid in cancer drug selection
AbstractGlobal high-throughput phosphoproteomic profiling is increasingly being applied to cancer specimens to identify the oncogenic signaling cascades responsible for promoting disease initiation and disease progression; pathways that are often invisible to genomics analysis. Hence, phosphoproteomic profiling has enormous potential to inform and improve individualized anti-cancer treatment strategies. However, to achieve the adequate phosphoproteomic depth and coverage necessary to identify the activated, and hence, targetable kinases responsible for driving oncogenic signaling pathways, affinity phosphopeptide enrichmen...
Source: Clinical Proteomics - December 19, 2022 Category: Biochemistry Source Type: research
