CSF extracellular vesicle proteomics demonstrates altered protein homeostasis in amyotrophic lateral sclerosis
ConclusionsProteomic analysis of CSF EVs in ALS detects intracellular alterations in protein homeostatic mechanisms, previously only identified in pathological tissues. This supports the wider use of CSF EVs as a source of novel biomarkers reflecting key and potentially druggable pathological intracellular pathway alterations in ALS. (Source: Clinical Proteomics)
Source: Clinical Proteomics - August 16, 2020 Category: Biochemistry Source Type: research
Quantitative analysis of differentially expressed proteins in psoriasis vulgaris using tandem mass tags and parallel reaction monitoring
ConclusionsThese findings confirmed that these proteins participate in the drug metabolism-other enzyme pathways and play crucial roles in the activation and proliferation of immune cells in the pathogenesis of PV. (Source: Clinical Proteomics)
Source: Clinical Proteomics - August 11, 2020 Category: Biochemistry Source Type: research
Proteomic analysis of synovial fluid from rheumatic arthritis and spondyloarthritis patients
ConclusionsThe proteomic results underline that neutrophils are central in the RA pathology but not in SpA, and even though inhibitors of neutrophils (migration, proteinase inhibitors) were present in the SF it was not sufficient to interrupt the disease process. (Source: Clinical Proteomics)
Source: Clinical Proteomics - August 5, 2020 Category: Biochemistry Source Type: research
Proteomic approaches for characterizing renal cell carcinoma
We describe the proteomic approaches utilized and relate the results of studies in the larger context of renal cell carcinoma biology. Moreover, we discuss some unmet clinical needs and how emerging proteomic approaches can seek to address them. There has been significant progress to characterize the molecular features of renal cell carcinoma; however, despite the large-scale studies that have characterized the genomic and transcriptomic profiles, curative treatments are still elusive. Proteomics facilitates a direct evaluation of the functional modules that drive pathobiology, and the resulting protein profiles would have...
Source: Clinical Proteomics - July 28, 2020 Category: Biochemistry Source Type: research
Using phosphoproteomics data to understand cellular signaling: a comprehensive guide to bioinformatics resources
AbstractMass spectrometry-based phosphoproteomics is becoming an essential methodology for the study of global cellular signaling. Numerous bioinformatics resources are available to facilitate the translation of phosphopeptide identification and quantification results into novel biological and clinical insights, a critical step in phosphoproteomics data analysis. These resources include knowledge bases of kinases and phosphatases, phosphorylation sites, kinase inhibitors, and sequence variants affecting kinase function, and bioinformatics tools that can predict phosphorylation sites in addition to the kinase that phosphory...
Source: Clinical Proteomics - July 10, 2020 Category: Biochemistry Source Type: research
The plasma peptides of sepsis
ConclusionSpecific proteins and peptides that vary between diseases might be discovered by the random and independent sampling of multiple disease and control plasma from different hospital and clinics by LC –ESI–MS/MS for storage in a relational SQL Server database and analysis with the R statistical system that will be a powerful tool for clinical research. The processing of SAA1 may play an unappreciated role in the inflammatory response to Sepsis. (Source: Clinical Proteomics)
Source: Clinical Proteomics - July 1, 2020 Category: Biochemistry Source Type: research
Identification of pathogens from native urine samples by MALDI-TOF/TOF tandem mass spectrometry
ConclusionsOur findings indicate that mass spectrometry has great potential as a reliable high-throughput tool for microbial pathogen identification in human urine samples. In this case, the MALDI-TOF/TOF, was used as an analytical tool for the determination of bacteria in urine samples, and the results obtained emphasize high importance of storage conditions and sample preparation method impacting reliability of MS2 data analysis. The proposed method is simple enough to be utilized in existing clinical settings and is highly suitable for suspected single organism infectious etiologies. Further research is required in orde...
Source: Clinical Proteomics - June 22, 2020 Category: Biochemistry Source Type: research
Laser capture microdissection coupled mass spectrometry (LCM-MS) for spatially resolved analysis of formalin-fixed and stained human lung tissues
ConclusionHerein we show that our multistep sample preparation methodology of LCM-MS can identify distinct, characteristic proteomic compositions of anatomical features within complex fixed and stained tissues. (Source: Clinical Proteomics)
Source: Clinical Proteomics - June 16, 2020 Category: Biochemistry Source Type: research
Activity-based protein profiling guided identification of urine proteinase 3 activity in subclinical rejection after renal transplantation
ConclusionsThere appear to be limited qualitative and quantitative differences in serine hydrolase activity in patients with subclinical versus clinical renal transplant rejection. The majority of enzymes identified were present in samples from both groups implying that in-gel quantitative differences may largely relate to the activity status of shared enzymes. However qualitative compositional differences were also observed indicating differential activities. The PR3/PRTN3 analyses indicate that the activity status of urine in transplant patients is dynamic possibly reflecting changes in the underlying processes in the tr...
Source: Clinical Proteomics - June 15, 2020 Category: Biochemistry Source Type: research
Human follicular fluid proteome reveals association between overweight status and oocyte maturation abnormality
ConclusionsOur work provided a new complementary high-confidence HFF dataset involved in oocyte maturation, and these altered HFF proteins might have clinical relevance and diagnostic and prognostic value for abnormal oocyte maturation in overweight status women. (Source: Clinical Proteomics)
Source: Clinical Proteomics - June 7, 2020 Category: Biochemistry Source Type: research
A comprehensive systematic review of CSF proteins and peptides that define Alzheimer ’s disease
ConclusionsOur study reveals a panel of 27 proteins and 21 peptides highly altered in AD with consistent statistical significance; this panel constitutes a potent tool for the classification and diagnosis of AD. (Source: Clinical Proteomics)
Source: Clinical Proteomics - June 4, 2020 Category: Biochemistry Source Type: research
Proteomic analysis of human synovial fluid reveals potential diagnostic biomarkers for ankylosing spondylitis
ConclusionWe performed quantitative comparative proteomic analysis using by LC –MS/MS of the SF from four disease states: RA, gout, and OA. This systematic comparison revealed novel differentially expressed proteins in AS SF, as well as two previously reported candidate biomarkers. We further verified the expression of MMP3, C9 and CFHR5 by western blot. These proteins may s erve as diagnostic or prognostic biomarkers in patients with AS, and may thus improve the clinical outcomes of this serious disease. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 31, 2020 Category: Biochemistry Source Type: research
Targeted mass spectrometry to quantify brain-derived cerebrospinal fluid biomarkers in Alzheimer ’s disease
ConclusionCollectively these findings highlight the utility of targeted MS approaches to quantify biomarkers associated with AD that could be used for monitoring disease progression, stratifying patients for clinical trials and measuring therapeutic response. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 28, 2020 Category: Biochemistry Source Type: research
New risk score for predicting steroid resistance in patients with focal segmental glomerulosclerosis or minimal change disease
ConclusionsOur study identified a 3-variable risk score in predicting steroid resistance in patients with FSGS or MCD. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 28, 2020 Category: Biochemistry Source Type: research
Identification of TUBB2A by quantitative proteomic analysis as a novel biomarker for the prediction of distant metastatic breast cancer
ConclusionsOur report is the first study to examine the distant metastatic breast cancer proteome using FFPE tissues. The depth of our dataset allowed us to discover a novel biomarker candidate and a proteomic characteristics of distant metastatic breast cancer. Distinct molecular features of various breast cancer subtypes were also established. Our proteomic data constitute a valuable resource for research on distant metastatic breast cancer. (Source: Clinical Proteomics)
Source: Clinical Proteomics - May 23, 2020 Category: Biochemistry Source Type: research
