Effect of hypoxia on the expression of microRNA in extracellular vesicles of human umbilical cord stem cells in vitro
In conclusion, this study demonstrated for the first time that microRNA expression in extracellular vesicles of human umbilical vein stem cells cultured under hypoxia is different from that under normal conditions, and these microRNAs may be markers for detecting hypoxia. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - May 24, 2023 Category: Stem Cells Source Type: research
Isolation and primary culture of human abdominal aorta smooth muscle cells from brain-dead donors: an experimental model for vascular diseases
AbstractPrimary cell cultures are essential tools for elucidating the physiopathological mechanisms of the cardiovascular system. Therefore, a primary culture growth protocol of cardiovascular smooth muscle cells (VSMCs) obtained from human abdominal aortas was standardized. Ten abdominal aorta samples were obtained from patients diagnosed with brain death who were organ and tissue donors with family consent. After surgical ablation to capture the aorta, the aortic tissue was removed, immersed in a Custodiol ® solution, and kept between 2 and 8 °C. In the laboratory, in a sterile environment, the tissue was fragmented a...
Source: Cell and Tissue Banking - May 5, 2023 Category: Stem Cells Source Type: research
Advantages and challenges in processing and quality control of decellularized heart valves
AbstractMore than 1000 donated aortic and pulmonary valves from predominantly European tissue banks were centrally decellularized and delivered to hospitals in Europe and Japan. Here, we report on the processing and quality controls before, during and after the decellularization of these allografts. Our experiences show that all tissue establishments, which provide native cardiovascular allografts for decellularization, meet comparably high-quality standards, regardless of their national origin. A total of 84% of all received allografts could be released as cell-free allografts. By far the most frequent reasons for rejecti...
Source: Cell and Tissue Banking - May 3, 2023 Category: Stem Cells Source Type: research
Comparison of demineralized bone matrix with different cycling crushing times in posterolateral fusion model of athymic rats
In conclusion, DMB with different cycling crushing times has no obvious difference in fusion rate of PLF, but it is slightly better than the ABG group. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - May 3, 2023 Category: Stem Cells Source Type: research
Insufficiency of collagenases in establishment of primary chondrocyte culture from cartilage of elderly patients receiving total joint replacement
Conclusions Collagenase IA was not sufficient in establishing primary human chondrocyte culture. Cartilage must be treated with pronaseE prior to collagenase IA application. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - May 3, 2023 Category: Stem Cells Source Type: research
Mesenchymal stem cells osteogenic differentiation by ZnO nanoparticles and polyurethane bimodal foam nanocomposites
In conclusion, Polyurethane nano scaffolds supported the MSCs ’ growth and rapid osteogenic differentiation. The PU-ZnO helps not only with cellular adhesion and proliferation but also with osteogenic differentiation. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - April 27, 2023 Category: Stem Cells Source Type: research
How to counteract the lack of donor tissue in cardiac surgery? Initial experiences with a newly established homograft procurement program
AbstractHomograft heart valves may have significant advantages and are preferred for the repair of congenital valve malformations, especially in young women of childbearing age, athletes and in patients with active endocarditis. A growing problem, however, is the mismatch between tissue donation and the increasing demand. The aim of this paper is to describe the initiation process of a homograft procurement program to attenuate the shortage of organs. A comprehensive description of the infrastructure and procedural steps required to initiate a cardiac and vascular tissue donation program combined with a prospective follow-...
Source: Cell and Tissue Banking - April 25, 2023 Category: Stem Cells Source Type: research
Clinical experience of reoperative right ventricular outflow tract reconstruction with valved conduits: risk factors for conduit failure in long-term follow-up
AbstractReconstruction of right ventricular outflow tract in patients with congenital heart disease in various age groups remains a controversial issue. Currently, a little is known about the fate of secondary and subsequent conduit. The aim of the study was to determine risk factors of conduit failure, evaluate long-term conduit survival, find out which type of conduit should be preferred in case of reoperations. We performed a retrospective analysis of a total of 249 records of valved conduit secondary and subsequent replacement in right ventricular outflow tract in 197 patients. Median follow-up was 5.7 years. The stu...
Source: Cell and Tissue Banking - April 21, 2023 Category: Stem Cells Source Type: research
Correction to: Impact of storage time prior to cryopreservation on mechanical properties of aortic homografts
(Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - April 17, 2023 Category: Stem Cells Source Type: research
The story of melanocyte: a long way from bench to bedside
This study aims at reviewing the history of melanocyte cultivation, and evaluating the effectiveness of transplantation of cultured cells. For this purpose, the authors examined the initial process of isolation, characterization, and transplantation of epidermal cells. This review, thus, summarizes the current understanding of the cutaneous pigmentary system from the start of synthesis in the pigment cells, along with the response of repigmentation. During the production of melanin, melanosomes are transferred to neighboring keratinocyte in order to form perinuclear melanin caps. The objective of this review is to analyze ...
Source: Cell and Tissue Banking - April 12, 2023 Category: Stem Cells Source Type: research
Suitable characteristics in the selection of human allogeneic chondrocytes donors to increase the number of viable cells for cartilage repair
AbstractAutologous chondrocyte implantation has shown optimal long-term outcomes in the treatment of cartilage lesions. The challenge for a single-stage approach lies in obtaining sufficient number of cells with high viability. The answer could lie in supplementing or replacing them with allogenic chondrocytes coming from cadaveric donors. In the present work, we aimed to compare the number of viable cells isolated from cartilage of live and cadaveric donors and to determine the suitable characteristics of the best donors. A total of 65 samples from donors aged from 17 to 55 years, either women or men, were enrolled in t...
Source: Cell and Tissue Banking - March 22, 2023 Category: Stem Cells Source Type: research
A bioscaffold of decellularized whole osteochondral sheet improves proliferation and differentiation of loaded mesenchymal stem cells in a rabbit model
This study aims to develop a new bioscaffold of decellularized osteochondral tissue (DOT) that is recellularized by bone marrow-derived mesenchymal stem cells (BM-MSCs), as a biphasic allograft, which preserved the interface between the cartilage section and subchondral bone of the joint. Whole osteochondral tissues of rabbit knee joints were sheeted in cartilaginous parts in 200 –250 µm sections while connected to the subchondral bone and then fully decellularized. The BM-MSCs were seeded on the scaffolds in vitro; some constructs were subcutaneously implanted into the back of the rabbit. The cell penetration, differe...
Source: Cell and Tissue Banking - March 20, 2023 Category: Stem Cells Source Type: research
Matched comparison of decellularized homografts and bovine jugular vein conduits for pulmonary valve replacement in congenital heart disease
AbstractFor decades, bovine jugular vein conduits (BJV) and classic cryopreserved homografts have been the two most widely used options for pulmonary valve replacement (PVR) in congenital heart disease. More recently, decellularized pulmonary homografts (DPH) have provided an alternative avenue for PVR. Matched comparison of patients who received DPH for PVR with patients who received bovine jugular vein conduits (BJV) considering patient age group, type of heart defect, and previous procedures. 319 DPH patients were matched to 319 BJV patients; the mean age of BJV patients was 15.3 (SD 9.5) years versus 19.1 (12.4) years ...
Source: Cell and Tissue Banking - March 14, 2023 Category: Stem Cells Source Type: research
Efficacy of autologous platelet-rich plasma for long-term glucocorticoids caused chronic wound: a case report
We report a case of a 42-year-old woman patient with a chronic hip wound combined with ischium bone exploration. And the patient who was treated with long-term glucocorticoids for rheumatoid arthritis has been through the experience of extensive conserva tive management. Thereafter necrosectomy and Vacuum-Assisted Closure (VAC) surgical procedure failed, and a PRP daily injection was performed at the ischial muscle and soft tissue. Neo-muscle appeared around the explored ischium bone after 8 weeks of injection and Complete wound healing was obta ined in 3 months. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - March 10, 2023 Category: Stem Cells Source Type: research
Characterization of three washing/decellularization procedures for the production of bioactive human micronized neural tissue (hMINT)
AbstractBackgroundWe developed a novel, injectable and decellularized human peripheral nerve-based scaffold, named Micronized Human Neural Tissue (hMINT), designed to be used as a supportive matrix for stem cell transplantation in the context of spinal cord injury (SCI).Materials and methodsHuman donated sciatic nerves were micronized at liquid nitrogen temperature prior to decellularization using 3 different procedures of various harshness. hMINT were characterized in terms of particle size, DNA, sulfated glycosaminoglycans (sGAG) and growth factors content. To test the biocompatibility and bioactivity of the various prep...
Source: Cell and Tissue Banking - February 28, 2023 Category: Stem Cells Source Type: research
