Heparin coated decellularized xenogeneic small diameter vascular conduit for vascular repair with early luminal reendothelialization
AbstractSmall diameter vascular graft is a clinical need in cardiovascular disease (CAD) and peripheral atherosclerotic diseases (PAD). Autologous graft has limitations in availability and harvesting surgery. To make luminal surface modification with heparin coating in xenogeneic small diameter vascular graft. We constructed a conduit from decellularized human saphenous vein (HSV) matrices in small diameter vascular graft (< 0.8 mm diameter). Luminal surface modification was done with heparin coating for transplantation in the rat femoral artery. Biocompatibility of conduit was checked in Chorioallantoic Membran...
Source: Cell and Tissue Banking - November 7, 2022 Category: Stem Cells Source Type: research
The potential for eye donation from hospice and palliative care clinical settings in England: a retrospective case note review of deceased patients' records
The objectives addressed in this paper are i.) to establish the size and describe the clinical characteristics of the potential eye donor population across six clinical sites; ii.) to identify challenges for clinicians in applying the standard ED criteria for assessing patient eligibility. Retrospective assessment of 1199 deceased patient case notes, 601 Hospice Care and 598 Hospital Palliative Care services, against current eye donation criteria. Clinicians ’ assessments were then evaluated against the same criteria. by specialists based at the National Health Service Blood and Transplant Tissue Services division (NHSBT...
Source: Cell and Tissue Banking - November 2, 2022 Category: Stem Cells Source Type: research
Test of the FlashFREEZE unit in tissue samples freezing for biobanking purposes
AbstractAvailability of molecularly intact biospecimens is essential in genetic diagnostics to obtain credible results. Integrity of nucleic acids (particularly RNA) may be compromised at various steps of tissue handling, and affected by factors such as time to freeze, freezing technique and storing temperature. At the same time, freezing and storing of the biological material should be feasible and safe for the operator. Here, we compared quality of DNA and RNA from biospecimens derived from different organs (breast, colon, adrenal glands, testes, rectum and uterus) frozen either using dry ice-cooled isopentane or with Fl...
Source: Cell and Tissue Banking - October 30, 2022 Category: Stem Cells Source Type: research
A novel 3D-electrospun nanofibers-scaffold grafted with Royal Jelly: improve hydrophilicity of the nanofibers-scaffold and proliferation of HUVEC cell line
In this study, a nanofibers-grafted scaffold was prepared with two-nozzle electrospinning to serve as a tissue engineering scaffold. Fundamental physical characterizations were carried out by scanning electron microscopy (SEM), pore diameter determination, and FT-IR. Fundamental physical characterization revealed that the nanofibers-scaffolds grafted with Royal Jelly significantly increased hydrophilicity, but the porosity of the novel-nanofibers did not alter significantly than the nanofibers without Royal Jelly. Based on the MTT assay results, cell growth, survival, and proliferation of the HUVEC Cell line were increased...
Source: Cell and Tissue Banking - October 25, 2022 Category: Stem Cells Source Type: research
Relative influence on cell behaviors of osteoblasts seeded onto demineralized bone matrix with diverse particle size
AbstractDemineralized bone matrix (DBM) is a natural and collagen-based allogeneic bone graft material with good bioactivity and biocompatibility. However, there are conflicting reports on the efficiency of this product owning to the relevant factors, especially, the particle size of DBM. In the current study, osteoblasts were seeded onto DBM with diverse particle sizes of 0.6 –1.0 mm, 2.2–2.6 mm, 3.8–4.2 mm and 5.4–5.8 mm, and then the cell adhesion, proliferation, differentiation and apoptosis were analyzed to evaluate the effects of particle sizes on bioactivity and biocompatibility of DBM. It could be ident...
Source: Cell and Tissue Banking - October 12, 2022 Category: Stem Cells Source Type: research
Netrin-1 promotes the vasculogenic capacity of human adipose-derived stem cells
AbstractAdipose derived stem cells (ADSCs) have been increasingly explored for use in cell-based therapy against ischemic diseases. However, unsatisfactory angiogenesis limits the therapeutic efficacy. Netrin-1, a known axon guidance molecule, improves neovascularization in the ischemic region. Thus, our study was performed to evaluate the potential effect of Netrin-1 on the angiogenic behaviors of human ADSCs (hADSCs). hADSCs acquired from human abdominal adipose tissue were modified by liposome transfection of Netrin-1 plasmid, and the proliferation of hADSCs was determined by Cell Counting Kit-8 (CCK-8) assay. The tr...
Source: Cell and Tissue Banking - October 12, 2022 Category: Stem Cells Source Type: research
Platelet-rich plasma: a comparative and economical therapy for wound healing and tissue regeneration
AbstractRise in the incidences of chronic degenerative diseases with aging makes wound care a socio-economic burden and unceasingly necessitates a novel, economical, and efficient wound healing treatment. Platelets have a crucial role in hemostasis and thrombosis by modulating distinct mechanistic phases of wound healing, such as promoting and stabilizing the clot. Platelet-rich plasma (PRP) contains a high concentration of platelets than naïve plasma and has an autologous origin with no immunogenic adverse reactions. As a consequence, PRP has gained significant attention as a therapeutic to augment the healing process....
Source: Cell and Tissue Banking - October 12, 2022 Category: Stem Cells Source Type: research
Cryopreserved human heart valve allografts: a ten-year single centre experience
This study provides an overview of tissue banking activities at the Croatian Cardiovascular Tissue Bank (CTB) during past ten years and presents the outcomes of cryopreserved heart valve allografts (CHAs) use in different patient groups. From June 2011 until December 2021, 75 heart donations were referred to CTB: 41 recipient of heart transplant (RHT), 32 donors after brain death (DBD) and 2 donors after circulatory death (DCD) donations. Processing resulted in 103 valves of which 65 met quality requirements for clinical use. Overall tissue discard rate was 37%. The most frequent reasons for discard were inadequate morphol...
Source: Cell and Tissue Banking - October 12, 2022 Category: Stem Cells Source Type: research
Exosome loaded hydroxyapatite (HA) scaffold promotes bone regeneration in calvarial defect: an in vivo study
In this study, hydroxyapatite (HA) scaffolds were synthesized and characterized, following the osteogenic and angiogenic effects of HA scaffolds with or without endometrial mesenchymal stem stromal cells (hEnSCs) derived Exosomes were investigated in rat animal model with calvaria defect. The X-ray diffraction (XRD) analysis of HA powder formation was confirmed with Joint Corporation of Powder Diffraction Standards (JCPDS) files numbers of 34-0010 and 24-0033A and Ball mill, and sintering manufactured Nano-size particles. Obtained results containing FE-SEM images presented that the surface of scaffolds has a rough and poro...
Source: Cell and Tissue Banking - October 3, 2022 Category: Stem Cells Source Type: research
RETRACTED ARTICLE: The effects of quercetin on wound healing in the human umbilical vein endothelial cells
(Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - September 22, 2022 Category: Stem Cells Source Type: research
The effects of quercetin on wound healing in the human umbilical vein endothelial cells
ConclusionIn conclusion, quercetin may serve as a potential bioactive substance that accelerates wound healing efficiently by modulating vasculogenezis and cells involved in healing. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - September 22, 2022 Category: Stem Cells Source Type: research
Clinical outcomes of long-term corneas preserved frozen in Eusol-C used in emergency tectonic grafts
AbstractTo report the clinical results on the use of corneas frozen in Eusol-C as tectonic corneal grafts.Retrospective review of medical records of patients who received frozen corneas as emergency tectonic grafts from 2013 to 2020. Corneas had been stored in Eusol-C preservation media at − 78 °C for a mean time of 6.9 months. Diagnosis, transplant characteristics, microbial culture results, anatomic integrity, epithelial healing, neovascularization, transparency, infection and need for additional surgeries were registered. Fifty corneas were used in 40 patients (mean age 6 0.5 years, 20 males) with a median fo...
Source: Cell and Tissue Banking - September 10, 2022 Category: Stem Cells Source Type: research
Improvement of human sperm properties with platelet-rich plasma as a cryoprotectant supplement
ConclusionsThis study showed a protective effect of PRP on human sperm quality at an optimized concentration after vitrification. Besides, the effects of PRP supplementation of sperms on successful fertility following sperm preservation will be of interest. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - September 8, 2022 Category: Stem Cells Source Type: research
Skin donations after suicide by acute poisoning
AbstractThe demand for skin tissue allografts to treat burns and other types of injuries increases each year to the extent that categories of donors formerly deemed “unsuitable”, such as victims of suicide by polytrauma or poisoning, are now considered. Patients who died by ingestion of/exposure to toxic substances can be accepted as tissue donors after assessment of graft safety to rule out any risks of transferring toxic substances to the recipient. A cad averic skin donation was obtained from a 57-year-old woman who died from intoxication after ingesting colchicine tablets (0.2 mg/kg). To determine the safety of ca...
Source: Cell and Tissue Banking - August 30, 2022 Category: Stem Cells Source Type: research
Osteogenic effect of electromagnetic fields on stem cells derived from rat bone marrow cultured in osteogenic medium versus conditioned medium in vitro
ConclusionThe synergistic effect of EMF and OM increased the expression ofRunx2 andOcn in rBMSCs. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - August 30, 2022 Category: Stem Cells Source Type: research
