Disinfection protocol for human musculoskeletal allografts in tissue banking using hydrogen peroxide 30%
AbstractMusculoskeletal allografts are used in reconstructive procedures, however, the risk of contamination with potential pathogens is possible, and safe transplantation requires multiple processing considerations. Hydrogen peroxide (H2O2) has commonly been used in bone washing because it can remove donor cells and eliminate antigens, pathogens, or cytotoxic agents from the matrix. The aim of this study was to evaluate the quantitative activity of H2O2 in a model of bone contamination with a high bacterial load to define the bioburden reduction. Twelve bone disc models were artificially contaminated withStaphylococcus au...
Source: Cell and Tissue Banking - June 6, 2021 Category: Stem Cells Source Type: research
In vitro differentiation effect of CCL4-induced liver injured mice serum on bone marrow-derived mesenchymal stem cells toward hepatocytes like cells
This study confirmed that CCl4-induced liver injured serum treatment can differentiate MSCs into hepatocyte-like cells in vitro. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - June 1, 2021 Category: Stem Cells Source Type: research
Regenerative medicine: characterization of human bone matrix gelatin (BMG) and folded platelet-rich fibrin (F-PRF) membranes alone and in combination (sticky bone)
AbstractDuring the last two decades autologous platelet and leukocyte rich products (PRP; PRF), opened new perspectives in regenerative medicine. In particular regenerative dentistry played a pioneer role in the application of these products in bone regenerative cases. Many aspects of cytokines, such as, growth factor release, blood cell content and its characterization were reported, but some practical questions are still unanswered in the preparation of PRF membranes and sticky bones. A new folding technique was introduced that created a good quality, pliable, and strong F-PRF membrane with a dense fibrin network and mor...
Source: Cell and Tissue Banking - June 1, 2021 Category: Stem Cells Source Type: research
Construction and a preliminary study of paracrine effect of bone marrow-derived endothelial progenitor cell sheet
AbstractThe release of paracrine factors from endothelial progenitor cell (EPC) sheet is a central mechanism of tissue repair. The purpose of this study was to constuct the rat bone marrow derived-endothelial progenitor cell (BM-EPCs) sheet and investigate invest the role of stromal cell-derived factor-1 α (SDF-1α)/CXCR4 axis in the biological function of BM-EPCs sheet. BM-EPC cells were identified by the cell-surface markers-CD34/CD133/VE-cadherin/KDR using flow cytometry and dual affinity for acLDL and UEA-1. After 7 days of incubation, the BM-EPC single-cell suspensions were seeded on thermo-s ensitive plate to harve...
Source: Cell and Tissue Banking - May 30, 2021 Category: Stem Cells Source Type: research
Anti-apoptotic effect of Nisin as a prebiotic on human mesenchymal stem cells in harsh condition
AbstractMesenchymal stem cells (MSCs) are progenitor cells of connective tissue with the ability of proliferation, self-renewal, and multilineage differentiation that make it a promising source with an enormous potential to be utilized for tissue repairing and vehicles of cell-based gene therapy. The low survival rate of MSCs following transplantation is their drawback. Preconditioning with some factors is a novel and effective strategy, improving the survival of the cells by protecting them from harmful conditions and result in the good recovery of injured tissues. Nisin is a prebiotic with antimicrobial activity. This ma...
Source: Cell and Tissue Banking - May 27, 2021 Category: Stem Cells Source Type: research
Corneal transplant during COVID-19 pandemic: the Italian Eye Bank national report
AbstractTo investigate the impact of Coronavirus Disease-2019 lockdown on the Italian Eye Bank organization. In this national retrospective, multicentric, cohort study, data from the Italian Eye Bank during both the lockdown and the first month after the lockdown period were retrieved. We compared the Italian Eye Bank metrics with the same timeframe of 2019 and 2018. Data from 13 out of 13 (100%) Italian Eye Banks were included in the analysis. A statistically significant reduction in the number of donor corneas retrieved in 2020 was found as compared to the same period in 2019 and in 2018, respectively (2020 = 1284; ...
Source: Cell and Tissue Banking - May 24, 2021 Category: Stem Cells Source Type: research
3D bioprinting in tissue engineering and regenerative medicine
AbstractThis review paper is primarily focused on bioprinting technology for biomedical applications. Bioprinting can be utilized for fabrication of wide range of tissue, based on which this chapter describes in detail its application in tissue regeneration. Further, the difficulties and potential in developing a construct for tissue regeneration are discussed herein. In this review paper, application of 3D bioprinting in tissue regeneration will be discussed in depth. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - May 22, 2021 Category: Stem Cells Source Type: research
Differentiation of human adipose-derived mesenchymal stem cells toward tenocyte by platelet-derived growth factor-BB and growth differentiation factor-6
In this study, we have corroborated that human ASCs are reactionary to tenogenic induction by GDF-6 and PDGF-BB alone or in combination. These outcomes will help greater insight into GDF-6 and PDGF-BB driven tenogenesis of ASCs and new directions of discovery in the design of ASC-based treatments for tendon healing. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - May 20, 2021 Category: Stem Cells Source Type: research
Correction to: Adipose tissue-derived stem cells: a comparative review on isolation, culture, and differentiation methods
In the original publication of this article the affiliation of the co-author Dr Azam Bozorgi was mistakenly published and the same has been corrected. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - May 13, 2021 Category: Stem Cells Source Type: research
Potential of a novel scaffold composed of human platelet lysate and fibrin for human corneal endothelial cells
AbstractCell-based therapies have been emerged to find innovative solutions for corneal endothelial dysfunction. The aim of this study is to investigate the suitability of a blended scaffold containing human platelet lysate (HPL) and fibrin not only for cultivating human corneal endothelial cells (HCECs) but also for serving as a scaffold for the respected cells. We isolated HCECs from human donors and encapsulated the cells with three concentrations of HPL/Fibrin scaffold, namely HPL/Fibrin 1, HPL/Fibrin 2 and HPL/Fibrin 3, by adding 28.9, 57.8 and 86.7 mg/dl of fibrinogen to HPL to obtain a final percentage of 10, 20 a...
Source: Cell and Tissue Banking - May 3, 2021 Category: Stem Cells Source Type: research
Sinasol versus Optisol-GS for cold preservation of human cornea: a prospective ex vivo and clinical study
AbstractTo compare ex vivo results of donor corneas maintained in Sinasol with those stored in Optisol-GS and reporting clinical outcomes of grafted Sinasol-versus Optisol-GS-stored corneas. In phase I, paired donor corneas were maintained in Sinasol or Optisol-GS. Afterward, the corneas were subjected to slit-lamp biomicroscopic and specular microscopic examinations on days 1 and 7, and then to trypan blue staining on day 7. The same examinations were performed on the corneas that were kept in Sinasol or Optisol-GS for 14 days. In phase II, the post-operative reports of 72 consecutive corneal transplantations were recor...
Source: Cell and Tissue Banking - May 2, 2021 Category: Stem Cells Source Type: research
A new isolation and culture method for granulosa cells
AbstractGranulosa cells are the cell population who have an increasing importance in the female genital system and reproduction. Thus, nowadays in vitro studies to address these cells are also gaining importance and attracts researcher ’s attention. The aim of our study is to develop a more feasible, low-cost granulosa cell isolation and culture method compared to methods defined so far. Granulosa cells were isolated from follicular fluids obtained from both healthy women donors (n = 19) and polycystic ovary syndrome (n = 15) applied to in vitro fertilization treatment process. Granulosa cells were isolated by u...
Source: Cell and Tissue Banking - April 29, 2021 Category: Stem Cells Source Type: research
Detection of the residual concentration of sodium dodecyl sulfate in the decellularized whole rabbit kidney extracellular matrix
Abstract To optimize rabbit kidney decellularization protocol, using sodium dodecyl sulfate (SDS) as a commonly used detergent, a methylene blue based assay was employed for detecting the minimum nontoxic SDS level for future cell seeding. The rabbit kidney tissues were decellularized with the perfusion-based method and underwent several investigations to determine the efficacy of decellularization in preserving the extracellular matrix (ECM) and cell removal. SDS detection was performed by incubating with methylene blue and subsequent extraction with chloroform. MTT (3-(4, 5-dimethylthiazol-2-yr)-2,5-diphenyltetrazolium b...
Source: Cell and Tissue Banking - April 28, 2021 Category: Stem Cells Source Type: research
Comparative evaluation of proliferative potential and replicative senescence associated changes in mesenchymal stem cells derived from dental pulp and umbilical cord
This study will thus pave the path of future research in selecting appropriate stem cell source for r egenerative medicine application. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - April 26, 2021 Category: Stem Cells Source Type: research
Effect of synthetic bone replacement material of different size on shear stress resistance within impacted native and thermodisinfected cancellous bone: an in vitro femoral impaction bone grafting model
AbstractAntibiotic carrier particles of variable size might influence mechanic properties within impacted thermodisinfected and native cancellous bone different. Herafill ®G containing calciumsulfate and calciumcarbonate provides high local concentrations of gentamicin being important for revision surgery in infected joint replacements. Native and thermodisinfected cancellous bone derived from 6 to 7 months old piglets was used for in vitro impaction bone grafting and supplemented each with Herafill®G granules of two different sizes. Micromovement of implants related to shear force was measured in 29 specimens distribut...
Source: Cell and Tissue Banking - April 24, 2021 Category: Stem Cells Source Type: research
