Peddling promise? An analysis of private umbilical cord blood banking company websites in Canada
The objective of this study was to analyze the marketing on the seven company websites offering private cord blood storage in Canada. We performed a mix of content and general qualitative analysis on the seven websites. Our analysis shows substantial hype around cord blood uses, amplifying the promise of speculative uses and distorting the likelihood of use. Findings show that this promotional messaging often deploys communication strategies which draw on testimonials and emotionally-charged nar ratives. Questions should be asked about whether the promissory aspects of these websites constitute breaches of Canadian law or ...
Source: Cell and Tissue Banking - April 23, 2021 Category: Stem Cells Source Type: research
Corneal endothelial cell therapy: feasibility of cell culture from corneas stored in organ culture
In conclusion, the CEC cultures required for endothelial cell therapy can be obtained from OC corneas without changing the standard storage operating procedures of the eye banks. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - April 16, 2021 Category: Stem Cells Source Type: research
Human amniotic membrane: an improvement in the treatment of Medication-related osteonecrosis of the jaw (MRONJ)? A case –control study
AbstractThe aim of this article is to report the results obtained by the use of HAM in surgical wound healing and the reduction of relapse in patients affected by Medication-related osteonecrosis of the jaw (MRONJ).The study involved patients with the diagnosis of MRONJ, surgically treated between October 2016 and April 2019, in a case –control setting. Enrolled patients were randomly divided into 2 groups. One group will be treated with resective surgery and with the insertion of HAM patch (Group A), while the second group had been treated exclusively with resective surgery (Group B).The patients underwent MRONJ surgica...
Source: Cell and Tissue Banking - April 15, 2021 Category: Stem Cells Source Type: research
Comparing the effects of Elaegnus Angustifolia , Hypericum Perforatum and Psidium Guajava extracts on metabolic activity of dental pulp-derived mesenchymal stem cells
AbstractDental pulp derived-mesenchymal stem cells (DP-MSCs) is considered a suitable are candidate for tissue engineering techniques and osseous reconstruction. Based on the hypothesis thatHypericum perforatum,Elaeagnus Angustifolia andPsidium guajava extracts can be used in cell-based bone tissue engineering due to meagre cytotoxicity response in the cell culture medium, their effects on the viability and metabolic activity of DP-MSCs were investigated and compared with each extract. DP-MSCs were extracted from human dental pulp, characterized by flow cytometry, and differentiated into Osteogenic and adipogenic lineages ...
Source: Cell and Tissue Banking - April 11, 2021 Category: Stem Cells Source Type: research
Schwann-like cell conditioned medium promotes angiogenesis and nerve regeneration
In conclusion, SCLC-CM promotes angiogenesis and nerve regeneration, it is expected to become a new treatment strategy for peripheral nerve injury. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - April 10, 2021 Category: Stem Cells Source Type: research
Human laryngeal squamous cell carcinoma cell line release of endogenous anandamide and 2-arachidonoylglycerol, and their antiproliferative effect via exogenous supplementation: an in vitro study
AbstractThe level of the major endocannabinoids anandamide (AEA) and 2-arachidonoylglycerol (2-AG) are altered in several types of carcinomas, and are known to regulate tumor growth. Thusly, this study hypothesized that the HEp-2 human laryngeal squamous cell carcinoma (LSCC) cell line releases AEA and 2-AG, and aimed to determine if their exogenous supplementation has an anti-proliferative effect in vitro. In this in vitro observational study a commercial human LSCC cell line (HEp-2) was used to test for endogenous AEA and 2-AG release via liquid chromatography-tandem mass spectrometry (LC –MS/MS). The anti-proliferativ...
Source: Cell and Tissue Banking - April 2, 2021 Category: Stem Cells Source Type: research
Decellularization of caprine esophagus using fruit pericarp extract of Sapindus mukorossi
AbstractBiological detergents like sodium deoxycholate, sodium dodecyl sulphate and Triton X-100 impairs the collagenous and non-collagenous proteins, glycosaminoglycans and growth factors. Further, certain chemical and enzymes are responsible for residual cytotoxicity in the decellularized extracellular matrix. The main focus of this study was to explore the decellularization property of soap nut pericarp extract (SPE) for development of decellularized tubular esophageal scaffold. For this 2.5, 5.0 and 10% concentrations of SPE were used for decellularization of caprine esophageal tissues. Histological analysis of hematox...
Source: Cell and Tissue Banking - March 26, 2021 Category: Stem Cells Source Type: research
Women ’s attitude towards umbilical cord blood banking in Poland
AbstractUmbilical cord blood (UCB) is considered as a valuable potential source of hematopoietic stem and progenitor cells. A process of collecting and storing UCB in the immediate period after the birth is called UCB banking. The study was conducted in order to determine women ’s knowledge, awareness, preferences and attitude towards UCB banking in Poland, considering the sociodemographic and obstetric factors. A cross-sectional, self-administered, online questionnaire-based study including mostly multiple choice questions concerning attitude and awareness regarding UCB banking was conducted entirely online among Facebo...
Source: Cell and Tissue Banking - March 22, 2021 Category: Stem Cells Source Type: research
Risk assessment in deferred and recovered donors
This study of 188 potential donor cases addresses issues of risk assessment in a medical examiner population in a metropolitan area, where serologic testing of deferred potential donors were compared with the DRAI screening, which determined the suitability or non-suitability for tissue procurement. Such serologic testing of deferred cases is not usually available in evaluating screening processes. This comparison gives insight into the effectiveness of the DRAI screening in deferring potential serology reactive donors. Results show in 65 cases how the DRAI screening eliminates m ost, but not all of the serologically rea...
Source: Cell and Tissue Banking - March 19, 2021 Category: Stem Cells Source Type: research
Human amniotic membrane mesenchymal stem cells exert cardioprotective effects against isoproterenol (ISO)-induced myocardial injury through suppression of inflammation and modulation of inflammatory MAPK/NF- κB pathway
In conclusion, our finding showed that intramyocardially administration of AMSCs could contribute to improvement of heart function and inhibition of inflammation in the site of injury by targeting inflammatory MAPK/NF-κB pathway. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - March 17, 2021 Category: Stem Cells Source Type: research
The risk of transmission of the novel coronavirus (SARS-CoV-2) with human heart valve transplantation: evaluation of cardio-vascular tissues from two consecutive heart donors with asymptomatic COVID-19
We report on two living donors of explanted hearts while receiving heart transplantation that tested positive for SARS-CoV-2 on the day of donation, although clinically asymptomatic. They underwent heart transplantation for ischaemic and hypertrophic obstructive cardiomyopathy, respectively. After evaluation of donor hearts, we cryopreserved and stored two pulmonary valves for clinical application and one aortic valve for research. Light microscopy of myocardium, mitral valve and aortic and pulmonary arterial wall and RT-PCR SARS-CoV-2 test of myocardium, mitral and tricuspid valve and aortic wall for detection of SARS-CoV...
Source: Cell and Tissue Banking - March 9, 2021 Category: Stem Cells Source Type: research
Co ‐culture with Sirt1-overexpressed chondrocytes delays the nucleus pulposus cells degeneration
This study aimed to evaluate the effects of autologous facet joint chondrocytes (CHs) with Sirtuin 1 (sirt1)-overexpression on NPCs degeneration. We used human NPCs and CHs isolated from the patients ’ tissue and transduced CHs with the plasmid vector to overexpress the sirt1 gene. Further, NPCs were seeded as monolayers and treated with IL-1β to obtain the degeneration, and the sirt1-overexpressed CHs (sirt1-CHs) in the transwell insert were co-cultured in the same well. The NPCs’ degenera ted degree was determined by the levels of living cells, proliferation, p16, and collagen I/II, and aggrecan expression at the ti...
Source: Cell and Tissue Banking - March 8, 2021 Category: Stem Cells Source Type: research
Tissue engineered human ear pinna derived from decellularized goat ear cartilage: clinically useful and biocompatible auricle construct
AbstractSurgery of the entire ear pinna even today presents a challenge to reconstructive surgeons, in the absence of a universally acceptable, quality construct for clinical use. In this article, the authors present a technique to generate a flexible, human size ear with the aim to meet this limitation for ear reconstructive surgeries. The construct was engineered by using a decellularized goat ear cartilage. This was characterized by hematoxylin –eosin (H/E), diamidino-2-phenylindole (DAPI), Masson's trichrome (MT), Alcian Blue (AB) staining and Scanning Electron Microscopy (SEM) for extracellular matrix (ECM) analysis...
Source: Cell and Tissue Banking - March 3, 2021 Category: Stem Cells Source Type: research
The effect of cryoprotectant vehicle solution on cartilage cell viability following vitrification
AbstractOsteochondral allografts are often used to repair large articular cartilage defects to prevent or delay the onset of osteoarthritis. This approach is limited by the timely acquisition and use of allograft tissue since standard hypothermic protocols allow for a maximum storage of 4 weeks. Vitrification is a proven technique for the long-term preservation of cells and tissues, but requires careful determination of parameters to be successful, particularly for articular cartilage. One parameter that is infrequently considered is the choice of cryoprotectant vehicle solution. T he aim of this study was to evaluate th...
Source: Cell and Tissue Banking - February 25, 2021 Category: Stem Cells Source Type: research
Invalid results of quantitative HIV and HCV NAT from living, heart beating and after circulatory death donors
(Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - February 25, 2021 Category: Stem Cells Source Type: research
