Current concepts for tissue transplant services for developing countries
AbstractThe transplantation of tissues can save lives and re-establish vital functions, where no alternatives of comparable effectiveness exist. This has led to establishment of tissue transplantation as a successful practice worldwide; however, a great variability between countries remains in terms of donation levels, safety, quality of grafts and their efficacy. Tissue transplantation requires coordination of different agencies involved in the implementation of procurement, processing, storage and distribution of tissues and cells from different hospital units that perform surgical procedures with graft-type input requir...
Source: Cell and Tissue Banking - January 4, 2021 Category: Stem Cells Source Type: research
Stem cell-based therapy as a promising approach in Alzheimer's disease: current perspectives on novel treatment
AbstractAlzheimer's disease (AD) is a neuronal disorder with insidious onset and slow progression, leading to growing global concern with huge implications for individuals and society. The occurrence of AD has been increased and has become an important health issue throughout the world. In recent years, the care of more than 35 million patients with AD costs over $ 600 billion per year, it is approximately 1 percent of the global Gross Domestic Product. Currently, the therapeutic approach is not effective for neurological deficits especially after the development of these major neurological disorders. The discovery of the ...
Source: Cell and Tissue Banking - January 4, 2021 Category: Stem Cells Source Type: research
Stem cell-based models and therapies: a key approach into schizophrenia treatment
AbstractPsychiatric disorders such as schizophrenia can generate distress and disability along with heavy costs on individuals and health care systems. Different genetic and environmental factors play a pivotal role in the appearance of the mentioned disorders. Since the conventional treatment options for psychiatric disorders are suboptimal, investigators are trying to find novel strategies. Herein, stem cell therapies have been recommended as novel choices. In this context, the preclinical examination of stem cell-based therapies specifically using appropriate models can facilitate passing strong filters and serious exam...
Source: Cell and Tissue Banking - January 2, 2021 Category: Stem Cells Source Type: research
Using gelatin/curcumin nano-fiber membranes as scaffolds in a subcutaneous model for tissue engineered cartilages
AbstractEngineered cartilage has several applications in treating cartilage ossification, however, its use is restricted clinically. We explored the feasibility of engineered cartilage in constructing tissues using gelatin/curcumin nano-fiber membranes as scaffolds in subcutaneous models. We constructed cartilage with gelatin nano-fiber membrane (control group) and gelatin/curcumin nano-fiber membrane (experimental group) as scaffolds. After the material was implanted into the back of BALB/c mice, gross view observation was performed. Histological examination was performed 3 and 12 weeks after implantation in vivo, and c...
Source: Cell and Tissue Banking - January 2, 2021 Category: Stem Cells Source Type: research
Making a family decision to donate the brain for genomic research: lessons from the genotype-tissue expression project (GTEx)
This study sheds light on the attitudes and circumstances that influence decisions by families to donate the brain of a deceased family member for research. This study, a part of the Genotype-Tissue Expression (GTEx) project, interviewed families of patients who had authorized organ and/or tissue donation for transplantation. A total of 384 family decision makers (FDMs) who decided to donate organs and/or tissues for transplantation were also asked to donate to GTEx. Of these, 297 families were asked to donate their loved one ’s whole brain and 87 families responded to a hypothetical request for brain donation. The decis...
Source: Cell and Tissue Banking - January 2, 2021 Category: Stem Cells Source Type: research
Comparing the osteogenic potential of schneiderian membrane and dental pulp mesenchymal stem cells: an in vitro study
In this study, we aimed to compare the osteogenic potential of human maxillary schneiderian sinus membrane (hMSSM)-derived stem cells versus permanent teeth dental pulp stem cells (DPSCs). Both cells types were cultivated in osteogenic and non-osteogenic inductive media. Alkaline phosphatase (ALP) activity assay and quantitative real-time PCR analysis were carried out to assess osteogenic differentiation. We showed that ALP activity and osteoblastic markers transcription levels were more striking in hMSSM-derived stem cells than DPSCs. Our results highlight hMSSM-derived stem cells as a recommended stem cell type for usage...
Source: Cell and Tissue Banking - January 1, 2021 Category: Stem Cells Source Type: research
Protective effects of mesenchymal stem cells on ischemic brain injury: therapeutic perspectives of regenerative medicine
AbstractCerebral ischemic injury as the main manifestation of stroke can occur in stroke patients (70 –80%). Nowadays, the main therapeutic strategy used for ischemic brain injury treatment aims to achieve reperfusion, neuroprotection, and neurorecovery. Also, angiogenesis as a therapeutic approach maybe represents a promising tool to enhance the prognosis of cerebral ischemic stroke. Unfortunatel y, although many therapeutic approaches as a life-saving gateway for cerebral ischemic injuries like pharmacotherapy and surgical treatments are widely used, they all fail to restore or regenerate damaged neurons in the brain. ...
Source: Cell and Tissue Banking - November 24, 2020 Category: Stem Cells Source Type: research
Innovative use of increasing the width of attached gingiva using chorion membrane along with platelet rich fibrin membrane
AbstractWidth of attached gingiva plays an important role in maintaining periodontal health, provides stabilization of the gingival margin against frictional forces and aids in dissipating physiological forces exerted by the muscular fibers of the alveolar mucosa on the gingival tissues. Inadequate width of attached gingiva leads to attachment loss, soft tissue recession, and impedes oral hygiene measures. Hence, procedures using autografts and allografts have been proposed to augment the attached gingiva. Allografts like chorionic membrane (CM) are known to enhance release of growth factors, promote neovascularization, he...
Source: Cell and Tissue Banking - November 24, 2020 Category: Stem Cells Source Type: research
The useful agent to have an ideal biological scaffold
AbstractTissue engineering which is applied in regenerative medicine has three basic components: cells, scaffolds and growth factors. This multidisciplinary field can regulate cell behaviors in different conditions using scaffolds and growth factors. Scaffolds perform this regulation with their structural, mechanical, functional and bioinductive properties and growth factors by attaching to and activating their receptors in cells. There are various types of biological extracellular matrix (ECM) and polymeric scaffolds in tissue engineering. Recently, many researchers have turned to using biological ECM rather than polymeri...
Source: Cell and Tissue Banking - November 22, 2020 Category: Stem Cells Source Type: research
Osteogenically-induced exosomes stimulate osteogenesis of human adipose-derived stem cells
In conclusion, our research confirmed that comprised of hADSCs-Exos and hADSCs may provide a new therapeutic paradigm for bone tissue engineering. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - November 20, 2020 Category: Stem Cells Source Type: research
Dynamic assessment of human sperm DNA damage III: the effect of sperm freezing techniques
AbstractAmong the currently available strategies for sperm freezing, vitrification may be considered as the leading alternative to conventional cryopreservation. Nevertheless, a direct comparison of both techniques with respect to the iatrogenic sperm DNA damage has not been performed yet. As such, this study was focused to assess the static and dynamic behavior of human sperm DNA damage following thawing of cryopreserved or vitrified spermatozoa. Semen samples were obtained from fifty donors with a normal spermiogram, and divided into four aliquots. The first aliquot represented the neat sample. In the second aliquot the ...
Source: Cell and Tissue Banking - November 17, 2020 Category: Stem Cells Source Type: research
Treatment of cartilage defects by Low-intensity pulsed ultrasound in a sheep model
This study was designed to evaluate the effect of Lipus on articular cartilage defects in a sheep. Eight sheep were divided in to two groups. The animals received bilateraly, articular cartilage defects 4 mm in diameter and 2 mm in deep on the patellar groove and experimental groups were treated with intensity 200 mW/cm2, 20 min/day with low-intensity pulsed ultrasound for 2 month. Then both knee joints underwent surgery for remove of formed tissue sample from defects.The samples were evaluated by Quantitative real-time polymerase chain reaction (qRT-PCR), Safranin-o staining, Immunofluorescence Staining and Morpholo...
Source: Cell and Tissue Banking - November 11, 2020 Category: Stem Cells Source Type: research
Brain donation in the era of COVID 19: challenges to the harvest in the face of a pandemic
AbstractWe have experienced numerous new challenges during the process of brain harvesting in the period of COVID-19. Although brain harvests have continued successfully during this time period, the numerous uncertainties and challenges described in this paper have nearly derailed the process several times. While the interface of the medical profession with patients in the context of a pandemic has been well-documented on several fronts, and particularly for those health care workers on the front lines, we are not aware of any documentary accounts of the challenges facing research and tissue donation programs. With this pa...
Source: Cell and Tissue Banking - November 11, 2020 Category: Stem Cells Source Type: research
In vitro differentiation effect of CCL 4 -induced liver injured mice serum on bone marrow-derived mesenchymal stem cells toward hepatocytes like cells
This study confirmed that CCl4-induced liver injured serum treatment can differentiate MSCs into hepatocyte-like cells in vitro. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - November 10, 2020 Category: Stem Cells Source Type: research
Factors influencing endothelial cell density of corneas for transplantation
AbstractTo evaluate factors affecting corneal endothelial cell density (ECD) under enucleation and preservation time studies at Eye Bank of the Federal District of Brazil. We conducted a case–control study collecting data from 1128 corneas where death-to-enucleation time and enucleation-to-preservation time were within 24 h. Low cell count were those corneas with an ECD less than 2000 cells/mm2 and high cell count was defined as those with ECD greater than 2000 cells/mm2. We calculated the independent risk factors related to: cause of death, donor age, death-to-enucleation time, enucleation-to-preservation time and...
Source: Cell and Tissue Banking - November 9, 2020 Category: Stem Cells Source Type: research
