A new storage solution for the hypothermic preservation of corneal grafts: an experimental study
AbstractIn this experimental study we used for the first time Tiprotec® as a solution for corneal preservation and cold storage. We compared the resultant endothelial cell morphology and viability with this obtained after preservation of the ex-vivo corneas with both usual standard techniques: conventional cold storage (using Eusol-C) and organ culture. This prospective, in vitro, 3-armed parallel study was performed with the use of 90 porcine corneas (examined for their endothelial quality and transparency) randomly selected for preservation in three storage methods (each 30 corneas): organ culture, standard cold stor...
Source: Cell and Tissue Banking - May 25, 2020 Category: Stem Cells Source Type: research

The importance of brain banking for dementia practice: the first experience of Turkey
This study reports the results of the first brain tissue banking experience of Turkey in the Unit for Aging Brain and Dementia at Dokuz Eylul University, Department of Geriatric Medicine, Izmir. Here, we have briefly described our efforts on brain banking in our country, which consist of six brains from autopsies that had at least two years of clinical follow-up in the 2015 –2017 period. The evaluation led to the diagnosis of two Alzheimer’s disease (AD) with cerebral amyloid angiopathy, one AD with dementia with Lewy bodies, one corticobasal degeneration, one multiple system atrophy, one vascular dementia. We ...
Source: Cell and Tissue Banking - May 20, 2020 Category: Stem Cells Source Type: research

Current challenges and future trends in manufacturing small diameter artificial vascular grafts in bioreactors
AbstractCardiovascular diseases are a leading cause of death. Vascular surgery is mainly used to solve this problem. However, the generation of a functional and suitable substitute for small diameter (
Source: Cell and Tissue Banking - May 15, 2020 Category: Stem Cells Source Type: research

Poly-phosphate increases SMC differentiation of mesenchymal stem cells on PLGA –polyurethane nanofibrous scaffold
AbstractThe use of bioactive scaffolds in tissue engineering has a significant effect on the damaged tissue healing by an increase in speed and quality of the process. Herein, electrospinning was applied to fabricate composite nanofibrous scaffolds by Poly lactic-co-glycolic acid (PLGA) and Polyurethane (PU) with and without poly-phosphate (poly-P). Scaffolds were characterized morphologically by scanning electron microscope (SEM), and their biocompatibility was also investigated by SEM, protein adsorption, cell attachment and survival assays. The applicability of the scaffolds for bladder tissue engineering was also evalu...
Source: Cell and Tissue Banking - May 9, 2020 Category: Stem Cells Source Type: research

In vitro analysis of the influence of mineralized and EDTA-demineralized allogenous bone on the viability and differentiation of osteoblasts and dental pulp stem cells
AbstractGrafting based on both autogenous and allogenous human bone is widely used to replace areas of critical loss to induce bone regeneration. Allogenous bones have the advantage of unlimited availability from tissue banks. However, their integration into the remaining bone is limited because they lack osteoinduction and osteogenic properties. Here, we propose to induce the demineralization of the allografts to improve these properties by exposing the organic components. Allografts fragments were demineralized in 10% EDTA at pH 7.2 solution. The influence of the EDTA-DAB and MAB fragments was evaluated with respect to t...
Source: Cell and Tissue Banking - May 8, 2020 Category: Stem Cells Source Type: research

Ethical and deontological aspects of pediatric biobanks: the situation in Italy
AbstractWhile pediatric biobanks are a precious resource for scientific research to improve our understanding of genetic pathologies, the value of these studies should be considered together with the value of the privacy rights of pediatric donors, as they are particularly vulnerable and in many cases unable to discern the meaning of the donation of biological material and the related implications of the research. Thus this work calls for reflection on the numerous ethical and legal issues involved in the development and regulation of these biobanks. In particular, it explores what form of consent best balances the intangi...
Source: Cell and Tissue Banking - April 20, 2020 Category: Stem Cells Source Type: research

Influence of thermodisinfection on microstructure of human femoral heads: duration of heat exposition and compressive strength
This study examined the compression force of the central and representative peripheral regions of native and thermodisinfected human femoral heads to observe wether different duration and intensity of h eat exposure might alter mechanic behaviour. Slices from the equatorial region of human femoral heads were taken from each 14 native and thermodisinfected human femoral heads. The central area revealed a significantly higher compression force for native (p ≤ 0.001) and for thermodisinfected bone (p = 0.002 andp = 0.005) compared with peripheral regions since no relevant differenc...
Source: Cell and Tissue Banking - April 20, 2020 Category: Stem Cells Source Type: research

Establishment of a dynamic osteosarcoma biobank: Ruijin experience
AbstractOutcomes for patients with metastatic and recurrent osteosarcoma remain poor and a better understanding of the biology of this malignancy is critical to the development of prognostic biomarkers and novel therapies. The purpose of this study was to establish a biobank of osteosarcoma which has the potential of monitoring tumors dynamically with exosomes, to facilitate clinical and basic scientific research. The osteosarcoma biological specimen and clinical data of osteosarcoma were collected in Ruijin Hospital in two stages. In the first stage (2015 –2017), the collection of tissue specimens and blood samples ...
Source: Cell and Tissue Banking - April 15, 2020 Category: Stem Cells Source Type: research

Virtual global biorepository: access for all to speed-up result-oriented research
AbstractBiorepositories have been established and used for the collection, storage, and distribution of biological materials for research and improving health. The terms “biobank” and “biorepository” are often used interchangeably, and they have been employed for more than a century to maintain and reallocate the biospecimens. The biorepository begins with the aim of data collection and the expected future use of human biological materials/specimens. Biorepo sitory on cancer, diabetes, infectious disease, neurological disorders, syndromes are established but they are located in the developed world. ...
Source: Cell and Tissue Banking - April 8, 2020 Category: Stem Cells Source Type: research

The effect of cryopreservation on DNA methylation patterns of the chromosome 15q11 –q13 region in human spermatozoa
In conclusion, based on our study, clinical use of sperm cryopreservatio n for fertility treatments appear to be safe in regard to DNA methylation in the chromosome 15q11–q13 region. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - April 6, 2020 Category: Stem Cells Source Type: research

Stem cells and vascular dementia: from basic science to the clinic
AbstractVascular dementia (VD) is the second most common cause of dementia following Alzheimer ’s disease (AD). The major symptoms of VD including memory loss, language deficits and impairment of executive functions. Its specific etiology and pathogenesis remain unknown. Currently, treatment options of VD are still limited. The therapeutic strategies aim to control the vascular risk factors and improve the cognitive function. In recent years, cell therapy for neurodegenerative diseases has attracted a great deal of attention. Evidence suggested that stem cell transplantation could improve the symptoms of cerebral inf...
Source: Cell and Tissue Banking - April 4, 2020 Category: Stem Cells Source Type: research

Positive toxicology and reactive serology in tissue donors: a retrospective study over a 3-year period
AbstractAssessment of donor suitability and criteria development for tissue donation evaluation which appropriately addresses the risk factors for disease transmission, especially high risk for Hepatitis B or C, HIV or other transmissible diseases as defined by the Food and Drug Administration, FDA, is a continuing concern for tissue banks. The relationship of drug use, especially IV drugs, has been determined to be associated with an increased possibility of reactive serology (Centers for Disease Control and Prevention (USCDC) in Division of Viral Hepatitis, National Center for HIV/AIDS, Viral Hepatitis, STD, and TB Preve...
Source: Cell and Tissue Banking - April 3, 2020 Category: Stem Cells Source Type: research

Neonatal Organ and Tissue Donation for Research: Options Following Death by Natural Causes
We report on the recovery of neonatal organs and tissues for research. A working group made up of bioethicists, neonatologists, lawyers, o bstetric practioners as well as organ procurement and tissue banking professionals evaluated legal, ethical and medical issues. Neonatal donor family members were also consulted. Our primary goals were (a) to ensure that referrals were made in compliance with all applicable federal and state laws, r egulations and institutional protocols, and (b) to follow acceptable ethical standards. Algorithms and policies designed to assist in the evaluation of potential neonatal donors were develop...
Source: Cell and Tissue Banking - March 13, 2020 Category: Stem Cells Source Type: research

Comparative study between topically applied irradiated human amniotic membrane in combination with tea tree oil versus topical tioconazole in pityraisis versicolor treatment
This study subjected 120 patients with hypopigmented or hyperpigmented PV lesions; half patients were treated weekly with TOSHAM compared with the others who applying 1% Tioconazole cream daily as a traditional treatment. Microbiological evaluation of in vitro fungicidal activity of TOSHAM versus Tioconazole was carried out againstMalassezia furfur culture. The clinical outcomes of this study proved the superior activity of TOSHAM to heal PV lesions than Tioconazole; this was in harmony with microbiological findings. This study approached a novel therapeutic treatment of PV with great outcomes by using TOSHAM. (Source: Cel...
Source: Cell and Tissue Banking - March 12, 2020 Category: Stem Cells Source Type: research

Mapping of proteomic profile and effect of the spongy layer in the human amniotic membrane
In this study we observed significant difference in the total amount of protein extract between the different donors. We do not observe a significant difference in the growth factor level between the conventional HAM and the spongy layer. N o variation was observed in the expression of HGF, KGF and NGF in different zone of HAM and neither between conventional HAM and spongy layer in each zone. (*p value
Source: Cell and Tissue Banking - March 12, 2020 Category: Stem Cells Source Type: research

Preliminary studies of hair follicle regeneration by injections of epidermal stem cells and dermal papilla cells into nude mice
This study emphasized that the rearrangement of the interactions during seed cells and the niches of the seed cel ls is essential and necessary for tissue-engineered construct success. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - March 11, 2020 Category: Stem Cells Source Type: research

Investigation on revascularization time and initial damage after transplantation of fresh and cryopreserved ovarian tissue in domestic cats
AbstractThe present study evaluated revascularization time of fresh and cryopreserved cat ovarian tissue after transplantation to subcutaneous tissue. Ovaries of five cats were used and eight pieces of ovarian tissue were taken from each pair of ovaries. Immediately after removal, three pieces were transplanted and one fixed for fresh control. The remaining four pieces were cryopreserved and, after thawing, one was fixed for cryopreservation control and three were transplanted. Grafts were recovered on days 2 (D2), 4 (D4) and 6 (D6) post-transplantation. Blood vessels were identified by immunohistochemistry and doppler ult...
Source: Cell and Tissue Banking - March 11, 2020 Category: Stem Cells Source Type: research

Knowledge and sources of information on umbilical cord blood donation in pregnant women
AbstractThe use of umbilical cord blood (UCB) holds promise for the treatment of a wide spectrum of diseases. However, information on UCB donation is not widespread or accurate among expectant women. The aim of this study is to evaluate pregnant women ’s knowledge of UCB donation, their main sources of information and their satisfaction with the knowledge possessed. Women (N = 375) in the last semester of pregnancy completed questionnaires evaluating sociodemographics, knowledge of UCB donation, past donation, sources of information, satisf action with the information and the desire to have received mor...
Source: Cell and Tissue Banking - March 11, 2020 Category: Stem Cells Source Type: research

Cell-based approaches towards treating age-related macular degeneration
AbstractAge-related macular degeneration as one of the most common causes of worldwide vision loss needs a proper approach for treatment. Therein, cell therapy and regenerative medicine can hold a great promise to be an effective approach. Accordingly, some preclinical and clinical studies were conducted to search around the therapeutic influence of stem cells in Age-related macular degeneration models and subjects. Hereupon, the purpose of the current review is to discuss the mechanisms of age-related macular degeneration, appropriate animal models along with suitable dosage and route of stem cell administration for its t...
Source: Cell and Tissue Banking - March 10, 2020 Category: Stem Cells Source Type: research

Patient attitudes towards brain donation across both neurodegenerative and non-neurodegenerative neurological disorders
AbstractBrain donations are imperative for research; understanding possible barriers to entry is required to improve brain donation rates. While a few surveys have studied attitudes towards brain banking in patients with neurodegenerative disorders, none have surveyed patients with chronic neurological disorders but without neurodegeneration. This cross-sectional study was conducted on 187 participants, with both neurodegenerative (n  = 122) and non-neurodegenerative disorders (n = 65), to compare their attitudes and preferences towards brain donation. Encouragingly, patients with non-neurodegen...
Source: Cell and Tissue Banking - March 5, 2020 Category: Stem Cells Source Type: research

The tolerance of human epidermal cells to trypsinization in vitro
In conclusion, the tolerance of human epidermal melanocytes to trypsinization in vitro was better than epidermal keratinocytes, and that property can be used to purify mela nocytes and was better than traditional differential trypsin digestion. The morphology of cells that survived the long-term trypsin digestion changed and they had good proliferative activity, but seemed to be more immature. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - February 27, 2020 Category: Stem Cells Source Type: research

Investigation of the mechanism of gamma irradiation effect on bovine bone
AbstractRadiation sterilization is an effective method of bone sterilization prior to bone graft transplantation. Gamma irradiation affects the biological and mechanical properties of bone; depending on the dose of radiation. The effect of gamma irradiation on bone mechanical properties is an unwanted phenomenon. However the mechanism of the effect of irradiation on bone mechanical properties is not properly understood. In this research paper the mechanism of the effect of gamma irradiation on bovine bone is investigated using scanning electron microscopy, energy-dispersive X-rays spectroscopy and Fourier transform infrare...
Source: Cell and Tissue Banking - February 17, 2020 Category: Stem Cells Source Type: research

Serological testing for infectious diseases markers of donor specimens from 24  h after death show no significant change in outcomes from other specimens
AbstractThere is increasing demand for organ and tissue donations to cater for a growing waiting list of recipients. Serological screening of donors remains the initial assessment upon which many decisions are made, particularly if donors are found to be seropositive. Multiple different platforms are now available, although the Abbott ARCHITECT platform assays are currently licensed globally for testing of blood collected at less than 15  h post-mortem. Compliance with the specified maximum collection times drastically decreases the number of eligible deceased donors, with ~ 70% more donations available if scre...
Source: Cell and Tissue Banking - February 12, 2020 Category: Stem Cells Source Type: research

Regeneration of sciatic nerves by transplanted microvesicles of human neural stem cells derived from embryonic stem cells
AbstractInjured nerves cannot regenerate on their own, and a lack of engraftable human nerves has been a major obstacle in cell-based therapies for regenerating damaged nerves. A monolayer culture approach to obtain adherent neural stem cells from human embryonic stem cells (hESC-NSCs) was established, and the greatest number of stemness characteristics were achieved by the eighth generation of hESC-NSCs (P8 hESC-NSCs). To overcome deficits in cell therapy, we used microvesicles secreted from P8 hESC-NSCs (hESC-NSC-MVs) instead of entire hESC-NSCs. To investigate the therapeutic efficacy of hESC-NSC-MVs in vitro, hESC-NSC-...
Source: Cell and Tissue Banking - February 12, 2020 Category: Stem Cells Source Type: research

Evaluation of the biofilm formation of Staphylococcus aureus and Pseudomonas aeruginosa on human umbilical cord CD146+ stem cells and stem cell-based decellularized matrix
This study aims to evaluate the CD146+ stem cells obtained from the human umbilical cord and their extracellular matrix proteins on in vitroPseudomonas aeruginosa andStaphylococcus aureus biofilms to understand their possible antimicrobial activity. CD146+ stem cells were determined according to cell surface markers and differentiation capacity. Characterization of the decellularized matrix was done with DAPI, Masson ’s Trichrome staining and proteome analysis. Cell viability/proliferation of cells in co-cultures was evaluated by WST-1 and crystal-violet staining. The effects of cells and decellularized matrix protei...
Source: Cell and Tissue Banking - February 4, 2020 Category: Stem Cells Source Type: research

Banking of corneal stromal lenticules: a risk-analysis assessment with the EuroGTP II interactive tool
AbstractWe evaluated the feasibility and performed a risk –benefit analysis of the storage and widespread distribution of stromal lenticules for clinical application using a new systematic tool (European Good Tissue and cells Practices II—EuroGTP II tool), specifically designed for assessing the risk, safety and efficacy of substances of human origin. Three types of potential tissue preparations for human stromal lenticules were evaluated: cryopreserved, dehydrated and decellularized. The tool helps to identify an overall risk score (0–2: negligible; 2–6: low; 6–22: moderate;>  22: h...
Source: Cell and Tissue Banking - February 4, 2020 Category: Stem Cells Source Type: research

Comparison of preloaded grafts for Descemet membrane endothelial keratoplasty (DMEK) in a novel preloaded transport cartridge compared to conventional precut grafts
AbstractTo determine the safety and graft quality of eye bank precut and preloaded grafts for Descemet membrane endothelial keratoplasty (DMEK) after storage and shipping in a novel preloaded transport cartridge compared to precut grafts in a conventional viewing chamber. In this laboratory proof-of-concept study, 29 human donor corneas that were unsuitable for transplantation with a mean endothelial cell density of 1948  ± 260 cells/mm2 were prepared using liquid bubble technique for producing precut lamellar grafts. The grafts were either preloaded into novel transport cartridge (n  =&thi...
Source: Cell and Tissue Banking - February 4, 2020 Category: Stem Cells Source Type: research

Hair follicle stem cells differentiation into bone cells on collagen scaffold
AbstractThe hair follicle is a dynamic structure which contains different niches for stem cells, therefore; it has been considered as valuable and rich sources of stem cells, due to easy access, multipotency and non-oncogenic properties. In the present study, the differentiation capacities of hair follicle stem cells into bone cells on the natural collagen scaffolds were investigated. The stem cells were extracted from the hair follicle bulge area of maleWistar rats ’ whisker and cultured until 3rd passage, then osteogenic differentiations were induced by culturing the cells in the specific osteogenic medium. After 3...
Source: Cell and Tissue Banking - February 3, 2020 Category: Stem Cells Source Type: research

In vitro susceptibility of microorganisms isolated from cold stored corneas: increased gentamicin-resistance in cornea banking
AbstractDonor cornea contamination is one of the major risks for corneal transplants. The use of antibiotics in storage media remains as one of the most important security measurements to minimize the contamination risk in corneal preservation. Since antibiotic resistance among microorganisms have been rising gradually, it is important to gain knowledge about the antimicrobial susceptibility pattern for choosing the most suitable antimicrobial agents. Thus, we evaluated the in vitro susceptibility of microorganisms isolated in donor corneas processed at the Center for Blood Transfusion, Tissues and Cells (C órdoba, ...
Source: Cell and Tissue Banking - January 24, 2020 Category: Stem Cells Source Type: research

Effect of the Cell Alive System on nerve tissue cryopreservation
AbstractEffective cellular cryopreservation while maintaining high cell viability is achieved by preventing intracellular and extracellular ice crystal formation using the Cells Alive System (CAS), a programmed freezer that applies a magnetic field. Here, the optimal temperature settings of the CAS were determined using rat sciatic nerves as a model tissue. Firstly, it was found that Schwann cell survival was increased by pre-cooling the samples in the ice crystal formation zone, increasing the freeze –thaw speed, and freezing–thawing in a magnetic field. Secondly, the setting (intensity and frequency) of the m...
Source: Cell and Tissue Banking - January 7, 2020 Category: Stem Cells Source Type: research

Proposed risk factors for failure of transplanted eye bank-prepared descemet stripping automated endothelial keratoplasty tissues
AbstractTo investigate the possible risk factors for failure of transplanted eye bank-prepared Descemet stripping automated endothelial keratoplasty (DSAEK) tissues. In a retrospective study between March 2011 and March 2019, all the failed DSAEK cases (131 cases) reported to the Central Eye Bank of Iran were compared with a surgeon-matched successful DSAEK group (control, 126 cases) in terms of the donor, DSAEK tissue, and recipient characteristics. Univariate analysis indicated that the DSAEK tissue preparation from excised corneoscleral tissues (OR 2.17;P = 0.026) and donor conjunctival hyperemia (OR 1.62;...
Source: Cell and Tissue Banking - January 6, 2020 Category: Stem Cells Source Type: research

The influence of donor and recipient characteristics on allograft tendons: a systematic review
AbstractTendon allograft has been an important alternative graft option aside from autograft. The outcome of reconstruction surgery is determined by donor and recipient related factors. The purpose of this article was to identified all studies reporting donor and recipient characteristics, including the age and gender of donors, along with the age, gender, activity level and smoking status of recipients, that affect the biomechanical properties and post-transplantation outcomes of allograft tendons. The systematic study search was based on MEDLINE via PubMed, Embase and the Cochrane Library databases. The reference lists o...
Source: Cell and Tissue Banking - January 2, 2020 Category: Stem Cells Source Type: research

Does bone marrow aspirate help enhance the integration of gamma irradiated allograft bone?
This study aimed to determine any enhancement effect when gamma sterilised allografts was impregnated with autologous bone marrow in improving the rate and quality of integration in metaphyseal-tibial defects of rabbits. Almost all subjects showed 50% of the defect being covered by new bones by the third week and smaller residual defect size in the treated group at the fifth week. Hounsfield units at the defect site showed increasing healing in all samples, with the treated group having an apparent advantage although insignificant (p  >  0.05). In the histopathological score evaluating healing over cortica...
Source: Cell and Tissue Banking - January 1, 2020 Category: Stem Cells Source Type: research

Which type of bone releases the most vancomycin? Comparison of spongious bone, cortical powder and cortico-spongious bone
AbstractBone infections can be challenging to treat and can lead to several surgeries and relapses. When a graft is needed, cavitary bone loss can be grafted with cancellous or cortical bone. Both can be used for grafting. However, the antibiotic releasing capacity of these grafts has not been compared. Which type of bone is best at releasing the most antibiotic has not been well established. The aim of this study was to determine which type of bone is best for antibiotic release when the bone is suffused with antibiotics by the surgeon. The hypothesis is that there would be a difference between the type of bone tested due...
Source: Cell and Tissue Banking - December 21, 2019 Category: Stem Cells Source Type: research

Comparison of the immunomodulatory effect of single MSC batches versus pooled MSC products
AbstractSevere corticosteroid-refractory graft-versus-host-disease (GVHD) is a major non-relapse cause of mortality and morbidity after an allogeneic hematopoietic stem cell transplantation (allo-HSCT). One of the most promising treatment options is using advanced therapy medicinal products based on mesenchymal stem cells (MSCs) immunomodulation ability. The protocols of MSC application differ in many parameters including a source of MSC, a dose, a number of doses or way of preparation of the medicinal product. The process is limited by the need for laborious and expensive manufacturing processes fraught with batch-to-batc...
Source: Cell and Tissue Banking - December 20, 2019 Category: Stem Cells Source Type: research

Factors associated with blood cord unit bankability: an analysis of a 15-year-long case series
This study aims to identify factors associated with the concentration of stem cells in umbilical cord blood, so increasing the probability of bankability, focusing on the possible implications in terms of obstetric and resources management. This is a retr ospective study conducted in the Obstetric Units of two Italian Hospitals in Montebelluna and Castelfranco Veneto. Study has been conducted on cord blood units banked between 1999 and 2015. Data on medical histories and clinical characteristics of mother and baby have been retrieved via a retrospect ive examination of medical records. A total of 869 cord blood units were ...
Source: Cell and Tissue Banking - December 18, 2019 Category: Stem Cells Source Type: research

Does myoinositol supplement improve sperm parameters and DNA integrity in patients with oligoasthenoteratozoospermia after the freezing –thawing process?
AbstractSperm cryopreservation is a routine method in andrology and IVF laboratory. However, the sperm quality and its fertilizing capacity have been decreased during this process. The purpose of this experiment was to determine the role of myoinositol as a supplement in amelioration of total and progressive sperm motility, DNA fragmentation, total antioxidant capacity (TAC), reactive oxygen species (ROS), and lipid peroxidation after the freezing –thawing process on patients with oligoasthenoteratozoospermia (OAT) syndrome. Semen samples obtained from 40 patients were divided into two aliquots and freezed with simpl...
Source: Cell and Tissue Banking - December 16, 2019 Category: Stem Cells Source Type: research

Rapid tissue processing using a temperature-controlled collection device to preserve tumor biomarkers
AbstractPrecision tissue diagnostics rely on high quality input specimens so that assay results are not affected by artifact, but advances in collection and processing of tissue specimens have lagged behind innovations in diagnostic assay development. Therefore, we have designed and evaluated a novel surgical tissue collection device that maintains and monitors sample temperature and motion throughout transport so that the major preanalytical variable of tissue temperature can be controlled and measured. This device, in combination with an improved cold –hot tissue fixation protocol affords optimal biomarker preserva...
Source: Cell and Tissue Banking - December 14, 2019 Category: Stem Cells Source Type: research

Interpretation of EBV serology for human body material donors: Is there a need for early antigen IgG and heterophile antibodies testing?
In this report we evaluated a diagnostic algorithm, proposed by the Belgian Superior Health Council, to detect acute and past Epstein –Barr virus (EBV) infections by means of serology in donors of human body material for transplantation. The available EBV serology parameters were tested on eighty serum samples on three random access analysers: Architect i2000 SR, Liasion XL and BioPlex 2200. The EBV sero-status was determined ac cording to the proposed algorithm and results were compared between the different analysers. Seventy one % of the samples gave concordant interpretations on the three analysers. Most of the d...
Source: Cell and Tissue Banking - December 14, 2019 Category: Stem Cells Source Type: research

Regional trends in corneal transplantation from 2004 to 2015 in France: a 12-year review on indications, technique and waiting period
AbstractThe aim of this study was to report the 12-year longitudinal trends in regional disparity in terms of indications, techniques and waiting period for corneal transplantation in France from 2004 to 2015. The records of all corneal transplantations performed from 2004 to 2015 in France were retrospectively reviewed by analasing the registry and the territorial organization [divided France into 7 interregional areas of collection and distribution of grafts (IACD)] of the French Biomedicine Agency. A total of 46,658 corneal transplantations were performed between 2004 and 2015. In 2014, there was 65.8 keratoplasties per...
Source: Cell and Tissue Banking - December 10, 2019 Category: Stem Cells Source Type: research

Characterization of progenitor/stem cell population from human dental socket and their multidifferentiation potential
AbstractDental stem cells have many applications in medicine, dentistry and stem cell biology in general due to their easy accessibility and low morbidity. A common surgical manoeuvre after a tooth extraction is the dental socket curettage which is necessary to clean the alveolus and favour alveolar bone healing. This procedure can cause very low morbidity compared to bone marrow collection procedures and the collected material is normally discarded. In order to investigate if the tissue obtained by dental socket curettage after a tooth extraction was a feasible alternative source to isolate human stem cells, we isolated a...
Source: Cell and Tissue Banking - December 5, 2019 Category: Stem Cells Source Type: research

Influence of cryopreservation on structure and function of mammalian spermatozoa: an overview
AbstractCryopreservation is a useful approach to preserve male fertility for assisted reproduction technique and other evaluations. In spite of extensive development in the cryopreservation field, there are biological and biochemical points including DNA fragmentation and antioxidant which should be illuminated to preserve fertility in safe form. Several molecular markers such as coding and noncoding RNAs are transferred from spermatozoa into oocyte via fertilization. These biomarkers affect fertility potential during the cryopreservation. Despite its impact, sperm cryopreservation has some destructive effect including los...
Source: Cell and Tissue Banking - December 3, 2019 Category: Stem Cells Source Type: research

Banking of human ovarian tissue potentially contaminated by cancer cells: experimental model for study of cryo-stability of these cells
This study aims to develop a suitable experimental model for cryobiological investigations of compacted cancer cells obtained after in vitro culture of a cell suspension. Suspended lineal breast cancer cells (ZR-75-1 and MDA-MB-231) were in vitro cultured in AIM V medium for formation of monolayer. Evaluation of the cell viability was performed by healing assay, transmembrane cell migration, invasion assay and immunofluorescent test of F-actin. It was established the possibility of formation of monolayer from viable cancer cells, scarification of monolayer of these cells and formation of compacted fragments. It is describe...
Source: Cell and Tissue Banking - November 28, 2019 Category: Stem Cells Source Type: research

A new approach to extend the storage of donor corneas after 28  days of corneal culture in an Italian eye bank
This study aimed to evaluate the possibility to extend the storage of unused organ-cultured donor corneas. After 28 days of corneal culture in TISSUE-C (AL.CHI.MI.A. S.R.L., Italy) and 5-day storage in transport/deswelling medium CARRY-C (AL.CHI.MI.A. S.R.L., Italy), 25 corneas that were deemed suitable for transplantation were transferred in fresh TISSUE-C at 31  °C for additional 7 days and then in fresh CARRY-C at room temperature for 24 h. Tissues were assessed for endothelial cell density (ECD), endothelial mortality and morphology after the standard and the extended corneal storage. In addition, th...
Source: Cell and Tissue Banking - November 28, 2019 Category: Stem Cells Source Type: research

Effect of glycerol concentrations and temperatures on epidermal growth factor protein expression in preserved canine amniotic membrane
This study aimed to study protein expression of EGF in CAM preserved with different concentrations of glycerol and storage temperatures, using enzyme-linked immunosorbent assay. CAM preserved in 50% glycerol and 99.5% glycerol and kept at 4 and − 20 °C for 7–30 days were compared. We found that preserving membrane with 50% glycerol at − 20 °C has significantly higher EGF protein expression compared with that at 4 °C (p 
Source: Cell and Tissue Banking - October 14, 2019 Category: Stem Cells Source Type: research

Potential of breastmilk in stem cell research
AbstractBreastmilk is a dynamic, multi-faceted, and complex fluid containing a plethora of biochemical and cellular components that execute developmental effects or differentiation program, providing nourishment and immunity to newborns. Recently, it was reported that breastmilk contains a heterogeneous population of na ïve cells, including pluripotent stem cells, multipotent stem cells, immune cells, and non-immune cells. The stem cells derived from breastmilk possess immune privilege and non-tumorigenic properties. Thus, breastmilk may represent an ideal source of stem cells collected by non-perceive procedure t han...
Source: Cell and Tissue Banking - October 12, 2019 Category: Stem Cells Source Type: research

Decellularization, cross-linking and heparin immobilization of porcine carotid arteries for tissue engineering vascular grafts
This study indicated the heparinized cross-linked vascular scaffolds may be ideal scaffolds for TEVGs. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - October 12, 2019 Category: Stem Cells Source Type: research

The effect of different cross-linking conditions of EDC/NHS on type II collagen scaffolds: an in vitro evaluation
AbstractThe purpose of this paper is to analyze the properties of porcine cartilage type II collagen scaffolds crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxy-succinamide (EDC/NHS) under different conditions. The porous EDC/NHS-crosslinked scaffolds were obtained through a two-step freeze-drying process. To determine the optimal crosslinking condition, we used different solvents and various crosslinking temperatures to prepare the scaffolds. Three crosslinking solutions were prepared with different solvents, photographs were taken with a flash in the darkroom, and light transmission was observed. ...
Source: Cell and Tissue Banking - October 3, 2019 Category: Stem Cells Source Type: research

Attitudes of Asian Parkinson patients towards brain donation
AbstractHistopathological examination of brain tissue is required for better understanding of neurodegenerative conditions such as Parkinson ’s disease and related disorders. However, patient willingness remains the greatest hurdle hampering participation in brain donation for research. While there is extensive research being conducted on the subject in West, to the best of our knowledge, there are no studies done in this regard in Asi a. This cross-sectional survey was conducted on 105 Parkinson’s disease patients to assess their knowledge, beliefs and attitude towards brain donation in an Asian population. Th...
Source: Cell and Tissue Banking - October 3, 2019 Category: Stem Cells Source Type: research

Rolling –sliding load decreases the loss of chondrocyte viability and the mechanical properties of cartilage explants preserved in vitro
In this study, we observed the effects of mechanical stimulation on chondrocyte viability and the mechanical properties of cartilage explants preserved in vitro using a rolling –sliding loading device designed by us, and the optimal stimulation protocol was established. A cylindrical osteochondral mass drilled on the femoral condyle of a healthy pig was divided into two groups (loading group and control group), and changes in the chondrocyte survival rate, matrix composi tion and cartilage biomechanical properties was observed at different time points. Additionally, the mRNA expression of the apoptosis-related protei...
Source: Cell and Tissue Banking - September 30, 2019 Category: Stem Cells Source Type: research