Impact of storage time prior to cryopreservation on mechanical properties of aortic homografts
The objective of this study was to analyze the mechanical properties of homograft tissue at different time spans in the procurement process. Ten aortic homografts were collected at the Tissue Bank in Lund. Twelve samples were obtained from each homograft, cryopreserved in groups of three after 2 –4 days, 7–9 days, 28–30 days, and 60–62 days in antibiotic decontamination. Mechanical testing was performed with uniaxial tensile tests, calculating elastic modulus, yield stress and energy at yield stress. Two randomly selected samples were assessed with light microscopy. Procurement gene rated a total of 120 samples, wi...
Source: Cell and Tissue Banking - February 27, 2023 Category: Stem Cells Source Type: research
Evaluation on the efficacy of processed hydrated and dehydrated amnion chorion membrane on the proliferation of periodontal ligament fibroblasts
AbstractThe purpose of the present study was to process and assess the effect of hydrated amnion chorion membrane and dehydrated amnion chorion membrane on proliferation of periodontal ligament (PDL) fibroblast cells. The amnion chorion membrane (ACM) from placenta of 18 systemically healthy patients was obtained from the Department of Obstetrics and Gynaecology. They were processed as hydrated and dehydrated based on different processing methods. The Periodontal ligament cells were o btained from periodontal ligament of freshly extracted premolars of systemically healthy patients, due to orthodontic reasons. The PD...
Source: Cell and Tissue Banking - February 25, 2023 Category: Stem Cells Source Type: research
Repair of tendons treated with peracetic acid –ethanol and gamma irradiation by EDC combined with NHS: a morphological, biochemical and biomechanical study in vitro
AbstractThe purpose of this study was to investigate whether 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) combined with n-hydroxysuccinimide (NHS) can repair tendon damage caused by peracetic acid –ethanol and gamma irradiation sterilization. The semitendinosus tendons of 15 New Zealand white rabbits were selected as experimental materials, and the tendons were sterilized in a solution containing 1% (v/w) peracetic acid and 24% (v/w) ethanol. After 15 kGy gamma irradiation sterilization, t he tendons were randomly divided into three groups (n = 10). The tendons were repaired with EDCs of 0, 2.5 and 5 mM com...
Source: Cell and Tissue Banking - February 16, 2023 Category: Stem Cells Source Type: research
Manufacturing of CD34 + HPC-enriched, high-purity mononuclear cell products from umbilical cord blood
AbstractThe purpose of this study was to explore methods of selectively enriching CD34 + haematopoietic progenitor cells (HPC) in mononuclear cell (MNC) preparations, and to outline a procedure for cryopreservation and thawing of manufactured material. Density gradient centrifugation of umbilical cord blood was achieved using Ficoll-Paque™ media at 1.077 g/mL and 1.065 g/mL d ensities and Leucosep preparation tubes. Post-process samples were analysed for CD34 + and MNC content. Finally, MNCs were frozen down at a concentration of 8.5 × 106 cells/mL in CryoStor CS10 using an Asymptote VIAFreeze controlled ...
Source: Cell and Tissue Banking - February 3, 2023 Category: Stem Cells Source Type: research
Preservation of human heart valves for replacement in children with heart valve disease: past, present and future
In this study, we review the impact of donor valve preservation on moving towards a more viable valve alternative for valve replacements in children or young adults. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - February 1, 2023 Category: Stem Cells Source Type: research
Role of secretomes in cell-free therapeutic strategies in regenerative medicine
AbstractAfter an injury, peripheral nervous system neurons have the potential to rebuild their axons by generating a complicated activation response. Signals from the damaged axon are required for this genetic transition to occur. Schwann cells (SCs) near a damaged nerve's distal stump also play a role in the local modulation of axonal programs,
not only via cell-to-cell contacts but also through secreted signals (the secretome). The secretome is made up of all the proteins that the cell produces, such as cytokines, growth factors, and extracellular vesicles. The released vesicles may carry signaling proteins as well a...
Source: Cell and Tissue Banking - February 1, 2023 Category: Stem Cells Source Type: research
The role of healthcare professionals to improve organ donation and transplantation outcome: a national study
ConclusionThe consent rate and donor management in the cases of brain death are unacceptable. The coordinators need training to increase their efficiency in terms of family approach and maintenance of brain death. Only by improving the level of family consent and increasing the coordinators' maintenance skills for brain death cases can the amount of organ donation in Iran be doubled to the current amount. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - January 28, 2023 Category: Stem Cells Source Type: research
Decellularized cryopreserved human pericardium: a validation study towards tissue bank practice
AbstractPericardial patches are currently used as reconstructive material in cardiac surgery for surgical treatment of cardiac septal defects. Autologous pericardial patches, either treated with glutaraldehyde or not, can be used as an alternative to synthetic materials or xenograft in congenital septal defects repair. The availability of an allogenic decellularized pericardium could reduce complication during and after surgery and could be a valid alternative. Decellularization of allogenic tissues aims at reducing the immunogenic reaction that might trigger inflammation and tissue calcification over time. The ideal graft...
Source: Cell and Tissue Banking - January 25, 2023 Category: Stem Cells Source Type: research
Correction to: Effect of multifactorial therapeutic approach on axonal regeneration and cell viability in an in-vitro model of spinal-derived neural injury
(Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - January 19, 2023 Category: Stem Cells Source Type: research
Management of grade II and III furcation defects with intramarrow penetration along with indigenously prepared DFDBA and amniotic membrane: a clinical and radiographic study
This study makes use of indigenously prepared demineralized freeze-dried bone allograft (DFDBA) and amniotic membrane (AM) as a cost-effective alternative. The purpose of the study was to compare the clinical outcome of grade II and III furcation defects with and without using indigenous DFDBA and AM prepared at Central Tissue Bank, MAIDS. 18 systemically healthy patients with chronic periodontitis displaying either grade II or III furcation defects were treated with open flap debridement (OFD) + intramarrow penetration (IMP) (control group) and OFD + IMP + DFDBA + AM (test group). The clinical and radiogr...
Source: Cell and Tissue Banking - January 10, 2023 Category: Stem Cells Source Type: research
Bone marrow mesenchymal stem cells ’ osteogenic potential: superiority or non-superiority to other sources of mesenchymal stem cells?
This study focuses on the specific potential and content of BMMSCs from which the specific capacity of these cells originates, and compares their osteogenic potential with other types of MSCs, and also the future directions in the application of BMMSCs as a source for cell therapy. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - January 9, 2023 Category: Stem Cells Source Type: research
Characterization of CM-Dil-labeled Muse cells in culture and in skin wounds in rats
AbstractTo investigate the characteristics of multilineage-differentiating stress-enduring (Muse) cells labeled with chloromethyl dialkylcarbocyanine (CM-Dil) in culture and in skin wounds of rats. Normal human dermal fibroblasts (NHDFs) were obtained from foreskins and were confirmed by immunocytochemistry with vimentin. Muse cells were derived from NHDFs using long-term trypsinization (LTT), were confirmed using immunocytochemistry with antibodies against stage specific embryonic antigen-3 (SSEA-3) and CD105 and were expanded in suspension cultures. The Muse cells were labeled with CM-Dil and were further evaluated with ...
Source: Cell and Tissue Banking - January 8, 2023 Category: Stem Cells Source Type: research
Vascular allografts for clinical application in Europe: assessment of 30 years of experience with vascular tissue banking in Brussels
AbstractVascular tissue banking has been carried out in Brussels for over 30 years in compliance with EU and Swiss tissue banking regulations. A total of 2.765 vascular tissue donations were performed in Belgian, French, Netherlands and Suisse transplant centres: 547(20%), 1.013(37%) and 1.205(43%) during the first, second and third periods, respectively. 85% and 18% incre ase in donations during the second and third decades compared to previous one, were remarkable. Of the 7.066 evaluated vascular tissues, 2.407(227, 921 and 1.259) were discarded (34.1%), whereas 4.659(523, 1.861 and 2.275) accepted (65.9%) during the r...
Source: Cell and Tissue Banking - January 3, 2023 Category: Stem Cells Source Type: research
Effects of ophthalmic surface anesthetic alcaine on the proliferation and apoptosis of human corneal endothelial cells through HIF-1 α regulation
This study explored the mechanism of alcaine in regulating HCE cells. HCE cell morphology under gradient concentrations was observed by an optical microscope. Cell proliferation and viability were detec ted by MTT assay to determine the half inhibitory concentration (IC 50). Cell apoptosis rate, HIF-1α mRNA expression, and HIF-1α, p/t-JNK and Caspase-3 protein levels were detected by flow cytometry, RT-qPCR, and Western blot. After treatment with alcaine at 0.625–5 g/L concentration range for 24 h, HCE cells showed cytoplasmic vacuolation, cell shrinkage, separation from culture matrix, and eventual death. Alcaine tr...
Source: Cell and Tissue Banking - December 26, 2022 Category: Stem Cells Source Type: research
Allogenic bone grafts and postoperative surgical site infection: are positive intraoperative swab cultures predictive for a higher infectious risk?
This study aims at determining whether positive spine allograft culture results are predictive of an increased risk of surgical site infection and whether they influence the surgeon’s choices in postoperative management. The records of 340 pati ents who received allogenic bone graft during spinal fusion surgery in our institution were reviewed, for a total of 677 allografts. Each graft was swabbed intraoperatively. All patients were followed clinically for postoperative complications. Infection was diagnosed based on clinical data, blood t ests and radiographic images, all assessed by an infectious disease specialist. ...
Source: Cell and Tissue Banking - December 26, 2022 Category: Stem Cells Source Type: research
