The effect of the anticoagulant on the cellular composition and growth factor content of platelet-rich plasma
ConclusionThe results of this study revealed that the use and the dose of SC affects the cellular content of PRP and GFs measured in fresh PRP. The CE and platelet dose increases while the WBC and VEGF decreases with the use of SC. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - August 28, 2021 Category: Stem Cells Source Type: research
The Dutch National TissueArchive Portal enables efficient, consistent, and transparent procurement of diagnostic tissue samples for scientific use
AbstractBiobanks play a crucial role in enabling biomedical research by facilitating scientific use of valuable human biomaterials. The PALGA foundation —a nationwide network and registry of histo- and cytopathology in the Netherlands—was established to promote the provision of data within and between pathology departments, and to make the resulting knowledge available for healthcare. Apart from the pathology data, we aimed to utilize PALGA’s nationwide network to find and access the rich wealth of Formalin-Fixed Paraffin-Embedded (FFPE) tissue samples for scientific use.
We implemented the Dutch National Tissue...
Source: Cell and Tissue Banking - August 25, 2021 Category: Stem Cells Source Type: research
Allograft bone banking experience in Pakistan
AbstractTo share our experience of establishing a bone bank in Pakistan, and the clinical use of these indigenously produced bone grafts. We retrospectively reviewed our experience of the procurement, processing, and storage of bone grafts at a bone bank in Karachi, Pakistan, the first bone bank to be established in a public sector hospital in Pakistan. The bone bank was established at Sindh Institute of Urology and Transplantation (SIUT), Karachi, in collaboration with Department of Orthopaedic Surgery, Dow University of Health Sciences/Civil Hospital, Karachi (CHK) in May, 2015. Since then, a large number of bone grafts ...
Source: Cell and Tissue Banking - August 20, 2021 Category: Stem Cells Source Type: research
Esophagus tissue engineering: from decellularization to in vivo recellularization in two sites
AbstractTo produce an esophageal scaffold with suitable features and evaluate the result of in vivo cell seeding after its implantation in the omentum and near its original anatomical position in the rat model. The esophagus of twelve rats were resected, cannulated, and decellularized via a peristaltic pump. After confirmation of decellularization and preservation of extracellular matrix, decellularized scaffolds were implanted either in the abdominal cavity (group I, n = 6) or cervical area (group II, n = 6). Histological evaluations were performed after 3 and 6 months of implantation. The results of histologica...
Source: Cell and Tissue Banking - August 19, 2021 Category: Stem Cells Source Type: research
Advanced backward planning with custom-milled individual allogeneic block augmentation for maxillary full-arch osteoplasty and dental implantation:a 3-year follow-up
AbstractIn the case of maxillary involution, augmentation is necessary for implant-supported prosthetics. The use of bone grafts is standard; customized allogeneic bone blocks may be a predictable alternative before dental implantation. For maxillary full-arch reconstruction, this case shows a horse-shoe augmentation by four allogeneic blocks, followed by guided dental implantation and fixed prosthetics after 6 months of healing. Using allogeneic blocks is an option for full-arch maxillary augmentation and comparable with autologous bone grafts. There is no donor site comorbidity. Bone height is stable for a minimum of ...
Source: Cell and Tissue Banking - August 9, 2021 Category: Stem Cells Source Type: research
The potential of radiation sterilized and banked tissue allografts for management of nuclear casualties
AbstractProcessed and radiation sterilized allograft tissues that can be banked for use on demand are a precious therapeutic resource for the repair or reconstruction of damaged or injured tissues. Skin dressings or skin substitutes like allograft skin, amniotic membrane and bioengineered skin can be used for the treatment of thermal burns and radiation induced skin injuries. Bone grafts can be employed for repairing fracture defects, filling in destroyed regions of bone, and treatment of spinal and joint injuries. A nuclear scenario would result in a large number of casualties due to the heat, blast and radiation effects ...
Source: Cell and Tissue Banking - July 31, 2021 Category: Stem Cells Source Type: research
Aesthetic and functional restoration of the dental arch continuity in difficult cases of impacted teeth within the frontal segment
AbstractImpacted teeth are a fairly common defect with the incidence ranging from 2.9 to 13.7%. Evaluation of the efficacy and safety of various treatment modalities in patients with impacted teeth within the frontal segment of the maxilla. The retrospective evaluation covered treatment outcomes in 81 patients with impacted teeth within the aesthetic segment. The subjects were divided into 3 groups. Patients with the impacted tooth uncovered and orthodontically repositioned in the arch were assigned to group 1. Group 2 consisted of patients with the impacted tooth qualified for extraction and the gap augmented and restored...
Source: Cell and Tissue Banking - July 14, 2021 Category: Stem Cells Source Type: research
Improved cell seeding efficiency and cell distribution in porous hydroxyapatite scaffolds by semi-dynamic method
AbstractTissue engineering is a promising technique for the repair of bone defects. An efficient and homogeneous distribution of cell seeding into scaffold is a crucial but challenging step in the technique. Murine bone marrow mesenchymal stem cells were seeded into porous hydroxyapatite scaffolds of two morphologies by three methods: static seeding, semi-dynamic seeding, or dynamic perfusion seeding. Seeding efficiency, survival, distribution, and proliferation were quantitatively evaluated. To investigate the performance of the three seeding methods for larger/thicker scaffolds as well as batch seeding of numerous scaffo...
Source: Cell and Tissue Banking - July 12, 2021 Category: Stem Cells Source Type: research
The biobank of barretos cancer hospital: 14 years of experience in cancer research
AbstractDespite the developments in cancer research over years, cancer is still one of the leading causes of death worldwide. In Brazil, the number of cancer cases for the several next years (2020 –2022) is expected to increase up to 625,000. Thus, translational research has been vital to determine the potential risk, prognostic, and predictive biomarkers in cancer. Therefore, Barretos Cancer Hospital implemented a biobank (BB-BCH) in 2006, which is responsible for processing, storage, and provision of biological materials from cancer and non-cancer participants. Hence, this article aimed to describe BB-BCH’s history, ...
Source: Cell and Tissue Banking - July 3, 2021 Category: Stem Cells Source Type: research
The quality assessment of the University hospital bone bank in Central Serbia: the second audit after fourteen years
AbstractWe analyzed the prevalence and predisposing factors for the overall rejection rate after retrieval of 267 fresh femoral head allografts over the past 7 years. The present study aimed to assess the quality system of institutional bone banking that can provide high-standard allografts with a low infection rate. Retrospective analysis of bone banking from June 2013 to December 2019 was conducted on 267 donors and 153 recipients. Of the 267 donated femoral heads, 74 were rejected, giving an overall rejection rate of 27.71%. The leading cause of allograft rejection was the inability to perform serology tests due to dono...
Source: Cell and Tissue Banking - July 2, 2021 Category: Stem Cells Source Type: research
Computer-assisted surgery with custom prostheses and human amniotic membrane in a patient with bilateral class IV TMJ reankylosis: a case report
AbstractA gold-standard technique has yet to be found for the treatment of temporomandibular joint ankylosis (TMJa), particularly in patients with recurring ankylosis. A 58-year-old male patient, with a history of multiple TMJ surgeries and severe limitation of mouth opening (maximum interincisal distance [MID] was 10 mm). Computerised tomography (CT) imaging highlighted a bilateral type IV ankylosis. The surgical guides were manufactured using a 3D printing me thod after obtaining a proper design of the osteotomy lines. The positioning of the fossa and condyle components of the custom TMJ prosthesis was digitally perfo...
Source: Cell and Tissue Banking - June 27, 2021 Category: Stem Cells Source Type: research
The effect of Scrophularia striata on cell attachment and biocompatibility of decellularized bovine pericardia
In this study, to increase cell attachment and ultimately cell proliferation on decellularized bovine pericardia,scrophularia striata extract was used.Scrophularia striata is an Iranian traditional medicinal plant. For this aim after decellularization of bovine pericardium and analysis of its morphology, it was incubated inscrophularia striata solution. Next, isolated human adipose-derived mesenchymal stem cells were cultured on the tissue. Finally, MTT assay, nitric oxide assay, and scanning electron microscopy observation were performed. MTT showed an increase in cell survival after treating the tissue with the plant e...
Source: Cell and Tissue Banking - June 26, 2021 Category: Stem Cells Source Type: research
Development of sheep secondary follicles and preservation of aromatase and metalloproteinases 2 and 9 after vitrification and in vitro culture
In conclusion, VSF are able to grow and develop during 12 days of in vitro culture and showed evidence of pre servation of steroidogenic function and remodeling of the basement membrane. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - June 21, 2021 Category: Stem Cells Source Type: research
Re-assessing the risk of undetected HBV, HCV and HIV in deceased tissue and living surgical bone donors in England
AbstractTesting of living surgical bone and deceased tissue donors by NHS Blood and Transplant (NHSBT) has included individual donation (ID) nucleic acid testing (NAT) for HBV, HCV and HIV since 2008. Here, the well-established window period methodology was used to estimate residual risk (RR). Prevalence of viral markers was calculated among both tissue donor populations. Incidence was derived by adjusting incidence among new blood donors by the prevalence ratio for tissue and new blood donors. Residual risk (RR) was calculated as the product of incidence and duration of WP for single donor HBV NAT at 0.058 years (21 da...
Source: Cell and Tissue Banking - June 14, 2021 Category: Stem Cells Source Type: research
Decellularized nerve extracellular matrix/chitosan crosslinked by genipin to prepare a moldable nerve repair material
AbstractDecellularized nerve extracellular matrix (NECM) composited with chitosan are moldable materials suitable for spinal cord repair. But the rapid biodegradation of the materials may interrupt neural tissue reconstruction in vivo. To improve the stability of the materials, the materials produced by NECM and chitosan hydrogels were crosslinked by genipine, glutaraldehyde or ultraviolet ray. Physicochemical property, degradation and biocompatibility of materials crosslinked by genipin, glutaraldehyde or ultraviolet ray were evaluated. The scaffold crosslinked by genipin possessed a porous structure, and the porosity ...
Source: Cell and Tissue Banking - June 11, 2021 Category: Stem Cells Source Type: research
