Compressive mechanical properties of vitrified porcine menisci are superior to frozen and similar to fresh porcine menisci
AbstractThe common practice of freezing meniscal allograft tissue is limited due to the formation of damaging ice crystals. Vitrification, which eliminates the formation of damaging ice crystals, may allow the mechanical properties of meniscal allograft tissue to be maintained during storage and long-term preservation. The primary objective of this study was to investigate the differences between fresh, frozen, and vitrified porcine lateral menisci examining compressive mechanical properties in the axial direction. Unconfined compressive stress-relaxation testing was conducted to quantify the mechanical properties of fresh...
Source: Cell and Tissue Banking - December 23, 2022 Category: Stem Cells Source Type: research
Drivers of consumers ’ behavioral intention toward private umbilical cord blood banking: a review
AbstractImmunitary bioeconomy encompasses a significant share of the bioeconomy that is accompanied by a high degree of complexity and various religious and ethical controversies for both customers and the service providers. Compared to blood banking, these complexities are more substantial for the new state-of-the-art technology of umbilical cord blood (UCB) banking, in which the viable th erapeutically active substance of cord blood (i.e., cord blood stem cells (CBSCs)) is banked for much less likely future demand. It became even more complicated when we knew that the main three types of cord blood bank...
Source: Cell and Tissue Banking - December 19, 2022 Category: Stem Cells Source Type: research
Regenerative medicine improve neurodegenerative diseases
AbstractRegenerative medicine is a subdivision of medicine that improves methods to regrow, repair or replace unhealthy cells and tissues to return to normal function. Cell therapy, gene therapy, nanomedicine as choices used to cure neurodegenerative disease. Recently, studies related to the treatment of neurodegenerative disorders have been focused on stem cell therapy and Nano-drugs beyond other than regenerative medicine. Hence, by data from experimental models and clinical trials, we review the impact of stem cell therapy, gene therapy, and nanomedicine on the treatment of Alzheimer ’s disease (AD), Parkinson’s dis...
Source: Cell and Tissue Banking - December 17, 2022 Category: Stem Cells Source Type: research
Effects of MRI on stemness properties of Wharton ’s jelly-derived mesenchymal stem cells
AbstractMesenchymal stem cells (MSCs), derived from various tissues, are served as a promising source of cells in clinic and regenerative medicine. Umbilical cord-Wharton ’s jelly (WJ-MSCs)-derived MSCs exhibit advantages over those from adult tissues, such as no ethical concerns, shorter population doubling time, broad differentiation potential, readily available non-invasive source, prolonged maintenance of stemness properties. The aim of this study was to evalua te the effect of MRI (1.5 T, 10 min) on stemness gene expression patterns (OCT-4, SOX-2, NANOG) of WJ-MSCs. Additionally, we assessed cell viability, growth...
Source: Cell and Tissue Banking - December 16, 2022 Category: Stem Cells Source Type: research
The promising approach of MSCs therapy for COVID-19 treatment
AbstractSeveral ongoing investigations have been founded on the development of an optimized therapeutic strategy for the COVID-19 virus as an undeniable acute challenge for human life. Cell-based therapy and particularly, mesenchymal stem cells (MSCs) therapy has obtained desired outcomes in decreasing the mortality rate of severe acute respiratory syndrome coronavirus 2 (SARS-COV-2), mainly owing to its immunoregulatory impact that prevents the overactivation of the immune system. Also, these cells with their multipotent nature, are capable of repairing the damaged tissue of the lung which leads to reducing the probabilit...
Source: Cell and Tissue Banking - December 16, 2022 Category: Stem Cells Source Type: research
STAT3 expression in patients with fragility fractures and its effect on the biological function of osteoblasts
AbstractTo determine the expression of signal transducer and activator of transcription 3 (STAT3) in patients with fragility fractures (FFs) and its effect on the biological function of osteoblasts. The study included 32 patients with FFs who were diagnosed and treated in the research group and 30 concurrent healthy individuals in the control group. We observed STAT3 mRNA expression in the patients with FFs and controls and altered STAT3 mRNA to detect changes in the proliferation, invasion, and apoptosis of osteoblasts. The patients with FFs presented higher serum STAT3 mRNA expression than the controls (P < 0.05)...
Source: Cell and Tissue Banking - December 12, 2022 Category: Stem Cells Source Type: research
Correction to: Effects of different preservation methods of human iliac veins
(Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - December 10, 2022 Category: Stem Cells Source Type: research
Low rate of detection of SARS-CoV-2 RNA in deceased tissue donors
AbstractGiven the possibility for disease transmission, this study was performed to determine whether there is detectable SARS-CoV-2 viral RNA in the blood of deceased tissue donors. A retrospective analysis of blood samples from eligible deceased tissue donors from Oct 2019 through June 2020 was performed. Plasma aliquots were initially tested with a SARS-CoV-2 NAT Assay; positive samples were further tested using an alternate NAT and an antibody assay. The proportion of donors with confirmed RNAemia and 95% confidence intervals were computed. Of donor samples collected in 2019, 894 yielded valid results, with 6 initially...
Source: Cell and Tissue Banking - December 9, 2022 Category: Stem Cells Source Type: research
Cord blood derived NK cells activated in counter with tumor cells
AbstractNK cells are initially known for their ability to kill tumor cells with no prior sensitization. Production of mature and long lasting NK cells from Umbilical Cord Blood (UCB) by using cytokines could be a promising method for immunotherapy. NK cells were generated from cord blood cells using IL2, IL7, and IL15 cytokines and measured expression of CD57 and NKp46 markers. Afterward, their capacity in the elimination of malignant cells (Reh cell line) was evaluated by assessment of interferon-γ (as cytokine production sign) and CD107-a expression (as cytotoxic function symptom) using flow cytometry. Our results showe...
Source: Cell and Tissue Banking - December 1, 2022 Category: Stem Cells Source Type: research
Fabrication of 3D oriented carbon nanofiber by two-nuzzle electrospinning as a cell scaffold
AbstractOne of the important issues in tissue engineering has been the development of 3D scaffolds, which guide cells to grow functional tissues and allow the diffusion of nutrients, metabolites, and soluble factors. Factors governing scaffold design include considerations of pore size and morphology, mechanical properties versus porosity, surface properties, and appropriate biodegradability. Three-dimensional structures with low density, high surface area and porosity can be utilized effectively in the tissue engineering. Recently two-nozzle electrospinning was used for fabricate polymeric and ceramic bulky layers with sp...
Source: Cell and Tissue Banking - December 1, 2022 Category: Stem Cells Source Type: research
Canadian demand and access to corneal transplantation: a provincial comparison
AbstractTo gather information from stakeholders involved in corneal donation and transplantation to inform discussion at the “National Consensus Forum on Improving Cornea Donation and Transplantation Access in Canada” held in February 2020, survey questions were posed to eye banks, transplanting ophthalmologists and organ donation organizations across Canada to learn more about demand, wait times, and access to tissue for transplant. The survey response rate was one hundred percent (100%) for eye banks and organ donation organizations while 64 percent (64%) of transplant ophthalmologists provided feedback. A number of ...
Source: Cell and Tissue Banking - November 20, 2022 Category: Stem Cells Source Type: research
Gram stain and addition of amphotericin B to improve the microbial safety of human donor corneas
AbstractTo determine the effectiveness of two methods to improve the microbial safety of human corneas preserved in organ culture. We compared the number of positive preservation solutions of corneas in organ culture in which the initial short-term hypothermic corneal maintenance solution was supplemented with amphotericin B 2.5 µg/mL and the historical data of microbial test results (2015–2019). In addition, we appraised the efficacy of Gram stain to detect bacterial or fungal contamination in the organ culture solutions of corneas from at-risk donors compared to the culture tests of corneas from not-at-risk donors. ...
Source: Cell and Tissue Banking - November 20, 2022 Category: Stem Cells Source Type: research
DNA banking, its current awareness and national status in biomedical research in India, a survey
This article highlights the current scenario concerning the general awareness, scientific consciousness and current national and internation al practices being initiated and in practice for the establishment of bio banking facilities particularly DNA bio banking. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - November 20, 2022 Category: Stem Cells Source Type: research
Long-term preservation of human donor corneal tissues in organ culture
(Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - November 20, 2022 Category: Stem Cells Source Type: research
Effect of multifactorial therapeutic approach on axonal regeneration and cell viability in an in-vitro model of spinal-derived neural injury
In this study, we used mixed neuronal-glial cultures, which are widely used for an in vitro study of neuronal damage. Scratch insult was made on cells using a cataract knife. The cells were divided into 8 groups (two control groups with and without olfactory ensheathing cells (OECs) treatment, injury group, three injury groups with single therapy by using super low dose of LPS (SLD-LPS) (100 pg/ml), OEC group, and G-CSF (100 ng/ml) group, and two injury groups with combined therapy (OEC with SLD-LPS and with all three treatments)). We found a significant decrease in the survival rate of injured cells (p < 0.001)...
Source: Cell and Tissue Banking - November 17, 2022 Category: Stem Cells Source Type: research
