New global reporting system for serious (product) events and adverse reactions in hematopoietic stem cell donation and transplantation
AbstractAnnually more than 21,000 volunteer unrelated hematopoietic stem cell donations are performed worldwide for patients with life-threatening diseases such as acute leukaemia. Donor safety issues and donor protection is one of the top priorities for the international cooperation of unrelated hematopoietic stem cell donor registries represented by the World Marrow Donor Association (WMDA). With this new global reporting system for serious events and adverse reactions (S(P)EARs), the WMDA aims to become the global leading online platform for hematopoietic stem cell donor organisations to report their S(P)EARs from both ...
Source: Cell and Tissue Banking - September 8, 2020 Category: Stem Cells Source Type: research
Use of collagen and auricular cartilage in bioengineering: scaffolds for tissue regeneration
AbstractThe aim of this study was the development of collagen and collagen/auricular cartilage scaffolds for application in dermal regeneration. Collagen was obtained from bovine tendon by a 72 h-long treatment, while bovine auricular cartilage was treated for 24 h and divided into two parts, external (perichondrium, E) and internal (elastic cartilage, I). The scaffolds were prepared by mixing collagen (C) with the internal part (CI) or the external part (CE) in a 3:1 ratio. Differentia l scanning calorimetry, scanning electron microscopy (SEM) analysis, microcomputed tomography imaging (micro-CT) and swelling degree we...
Source: Cell and Tissue Banking - September 1, 2020 Category: Stem Cells Source Type: research
Comparison of rapid freezing versus vitrification for human sperm cryopreservation using sucrose in closed straw systems
This study aimed to determine the optimal sucrose concentration for human sperm cryopreservation and compare the cryoprotective effects of rapid freezing versus vitrification using different closed straw systems in terms of sperm motility and DNA integrity. Our data showed that: (1) The optimal sucrose concentration for vitrification was 0.25 mol/l among the tested 0, 0.125, 0.25 and 0.5 mol/l concentrations; (2) Sperm total motility and progressive motility were cryopreserved significantly better by rapid freezing than vitrification in standard 0.5 ml cryostraws (P < 0.05); and (3) Sperm total motility and pro...
Source: Cell and Tissue Banking - August 31, 2020 Category: Stem Cells Source Type: research
Autologous mesenchymal stem cells application in post-burn scars treatment: a preliminary study
AbstractMesenchymal stem cells (MSCs) are multi-potent cells characterized by long term self-renewal and by potential for differentiation into cells of different mesenchymal tissue types such as fibroblasts, osteocytes, chondrocytes, and adipocytes. Their unique properties offer broad therapeutic potentials. Bone marrow has been used as the most common MSCs source, but it is gradually going to be replaced by adipose tissue which showed to contain more MSCs per unit than the bone marrow and clinical application of MSCs procured from the adipose tissue have been demonstrating at least similar results. Post-burn scars result ...
Source: Cell and Tissue Banking - August 29, 2020 Category: Stem Cells Source Type: research
A preliminary study on the development of a novel biomatrix by decellularization of bovine spinal meninges for tissue engineering applications
In conclusion, the in vitro prelim inary study has demonstrated that the dBSM might be a proper and new bioscaffold for tissue engineering and regenerative medicine applications. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - August 29, 2020 Category: Stem Cells Source Type: research
Comparison the effects of hypoxia-mimicking agents on migration-related signaling pathways in mesenchymal stem cells
This study provides credible evidence around DFX, which might be an effective drug for pharmacological preconditioning of Ad-MSCs to boost their homing capacity and regeneration of damaged tissues though, activation of the migration-related signaling pathways. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - August 18, 2020 Category: Stem Cells Source Type: research
Screening for pre-leukemia TEL-AML1 chromosomal translocation in banked cord blood units: cord blood bank perspective
AbstractAcute lymphocytic leukemia is the most common leukemia in children. Many studies suggest the existence of two subsequent hits in order for the disease to occur. TEL-AML1 (ETV6-RUNX1) is considered an initial genetic hit that occurs prenatally and generates a pre-leukemia clone. In cord blood (CB) stem cell transplantation, donor cell leukemia (DCL) is one of the complications associated with the presence of the pre-leukemic clone. The aim of this study was to identify the prevalence of ETV6-RUNX1 translocation in CB units and the feasibility in implementing such a screening test, to ensure the safety of the CB unit...
Source: Cell and Tissue Banking - August 17, 2020 Category: Stem Cells Source Type: research
Cryopreservation of peripheral blood mononuclear cells using uncontrolled rate freezing
In this study, we evaluated the performance of uncontrolled rate freezing and controlled rate freezing cooling methods through cell recovery rate, viability, differentiation potential into cytokine-induced killer cells and the cellular properties of the cultured cytokine-induced killer cells. The results showed similar post-thaw viability and recovery rate in both controlled rate freezing and uncontrolled rate freezing cryopreserved peripheral blood mononuclear cells. Importantly, the uncontrolled rate freezing cryopreserved peripheral blood mononuclear cells exhibited higher growth ratio and earlier cell clustering during...
Source: Cell and Tissue Banking - August 17, 2020 Category: Stem Cells Source Type: research
Determination of antibiotics and detergent residues in decellularized tissue-engineered heart valves using LC –MS/MS
AbstractResidual chemicals that are presented during tissue processing in human tissue banks can be a risk for the allograft recipient. Determine the residual concentrations of the antibiotics and detergent used in the process of human decellularized tissue-engineered heart valves stored in isotonic saline solution up to 18 months. A total of 24 human decellularized allografts were stored in sterile sodium chloride and analyzed immediately after the decellularization process (0 months) and after storage for 6, 12, and 18 months, which includes the use of sodium dodecyl sulfate (SDS) and antibiotics (cefoxitin, vanc omy...
Source: Cell and Tissue Banking - August 17, 2020 Category: Stem Cells Source Type: research
A novel alginate localization molding technique for cross-sectional area measurement of human tendon to access biomechanical properties
AbstractAccurate determination of the biomedical properties of connective tissue such as tendons and ligaments is dependent on the accurate measurement of their cross-sectional area (CSA). To date, techniques for determining cross-sectional areas of ligaments and tendons have been less than ideal due to their complex geometries and their deformations under external load. A novel non-destructive technique has been developed for determining the cross-sectional area of tendon by locating the tendon rupture, in which aqueous rapid curing alginate dental molding materials, digital photography and computerized image analysis are...
Source: Cell and Tissue Banking - August 16, 2020 Category: Stem Cells Source Type: research
The influence of preparation and storage time on endothelial cells in Quarter –Descemet membrane endothelial keratoplasty (Quarter–DMEK) grafts in vitro
In this study, we evaluated in vitro changes in endothelial cell distribution, viability and morphology of Quarter–DMEK graf ts when stored in organ-culture medium. Quarter–DMEK grafts were prepared from 5 corneas and stored in organ-culture medium for 4, 7 and 11 days. Endothelial cell re-distribution was investigated by light microscopy, cell viability by a Calcein-AM assay, and expression of endothelial and non-endo thelial markers by immunohistochemistry. Three standard DMEK-grafts were used as controls. After preparation, all Quarter–DMEK grafts showed a band with no viable endothelial cells along the radial cu...
Source: Cell and Tissue Banking - August 13, 2020 Category: Stem Cells Source Type: research
The top 100 most-cited articles citing human brain banking from 1970 to 2020: a bibliometric analysis
AbstractMany articles involving human brain banks have been published. Bibliometric analysis can determine the history of the development of research and future research trends in a specific field. Three independent researchers retrieved and reviewed articles from the Web of Science database using the following strategy: “TS = (((brain OR cerebral) AND (bank* OR biobank*)) OR brainbank*).” The top 100 most-cited articles were identified and listed in descending order by total citations. Web of Science was used to identify ten recent articles describing bank construction. GeenMedical (https://www.geenmedical.com/) w...
Source: Cell and Tissue Banking - August 5, 2020 Category: Stem Cells Source Type: research
Donation of discarded ocular tissue in patients undergoing SMILE laser refractive surgery: developing appropriate guidelines
AbstractTissue Biobanks represent an invaluable resource. Despite the majority of people supporting tissue donation, the actual rate remains low overall. Tissue discarded from surgical procedures represents a further avenue for collection for use in research. We aim to understand the information and consent requirements in a cohort of healthy, post-ophthalmic surgical subjects to optimise future tissue collection in living donors. Patients attending an ophthalmic clinic following refractive surgery for myopia (SMILE) were identified. Patient consent was implied with the completion of the provided survey. The questionnaire ...
Source: Cell and Tissue Banking - July 21, 2020 Category: Stem Cells Source Type: research
Understanding factors affecting human chondrocyte culturing: an experimental study
AbstractOver the years, surgical strategies have been developed in hope of full regeneration of the injured cartilage. In our study, we aimed to develop an optimized chondrocyte culture isolation technique as an active ingredient of a standardized autologous chondrocte implantation product, which is able to maintain the phenotype along with the molecular features of the cartilage. We compared different enzymes, which suggested optimal performance with collagenase type II at 5 mg/ml concentration. Thereafter, we observed that COL2 and GAG expression is substantially reduced with passaging. There was a need to omit passagi...
Source: Cell and Tissue Banking - July 14, 2020 Category: Stem Cells Source Type: research
Current clinical application of sclera and amniotic membrane for ocular tissue bio-replacement
AbstractTo report the current clinical applications and trends of scleral and amniotic membrane use in ophthalmology. Review of annual reports from the Catalan Transplant Organization (OCATT), on scleral patch and amniotic membrane eye indications in Catalonia region (Spain) over a 6-year period from 2013 to 2018. A total of 874 scleral and 1665 amniotic membranes patches were implanted, from January 2013 to December 2018. The most frequent indication over the 6-year period for scleral patch was glaucoma surgery (77.5%), eyelid reconstruction (5.2%) and corneal or scleral ulcer (5%). Regarding amniotic membrane, corneal ul...
Source: Cell and Tissue Banking - July 12, 2020 Category: Stem Cells Source Type: research
