Optimization of skeletal muscle-derived fibroblast isolation and purification without the preplating method
In this study, we optimized the isolation and purification methods for skeletal muscle-derived fibroblasts. These methods are expected to be used in various applications for tissue engineering. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - January 25, 2022 Category: Stem Cells Source Type: research
Collagen matrices are preserved following decellularization of a bovine bone scaffold
AbstractThe decellularization of bovine bone has emerged as a strategy for the repair, replacement, and regeneration of bone defects. To evaluate the effects of a new protocol of bone decellularization and its impact on the structure and collagen scaffold. Cancellous bone from bovine femur was dissected in fragments and decellularized based on protocol of multiple steps. The residual protein levels, histological, morphometric, and scanning electronic microscopy analyses were carried out to evaluate the effects of decellularization and the impact on the structure and collagen scaffold. A cytotoxicity assay was performed. Re...
Source: Cell and Tissue Banking - January 24, 2022 Category: Stem Cells Source Type: research
Approaches to reducing the toxic exposure hazard on the sheep population
AbstractThe sheep breeding industry forms a substantial proportion of the agriculture sector in the Republic of Kazakhstan and ensuring its sustainable functioning should be implemented in a number of national documents, which can be considered as the basis for increasing the level of national food security. This can be achieved not only by increasing livestock numbers, but also by conducting research that will reduce the incidence of disease in livestock. The novelty of the study is determined by the fact that in the extension study of autopsy of sheep affected by diseases, an analysis of factors that increase the inciden...
Source: Cell and Tissue Banking - January 24, 2022 Category: Stem Cells Source Type: research
Study of the biological characteristics of human umbilical cord mesenchymal stem cells after long-time cryopreservation
AbstractHuman umbilical cord mesenchymal stem cells (hUC-MSCs) have considerable potential in cell therapy. Cryopreservation represents the gold standard in cell storage, but its effect on hUC-MSCs is still not well understood. The aim of this study was to investigate the effect of one year of cryopreservation and thawing on the biological characteristics of hUC-MSCs from the same donors. Fresh hUC-MSCs were cryopreserved in commercial freezing medium (serum-free CellBanker 2) at passage 2. After one year of cryopreservation, the hUC-MSCs were thawed and subcultured to passage 4. The comparison was performed in terms of fo...
Source: Cell and Tissue Banking - January 23, 2022 Category: Stem Cells Source Type: research
Effects of mesenchymal stem cell culture on radio sterilized human amnion or radio sterilized pig skin in burn wound healing
In this study, we developed mesenchymal stem cells-cellularized skin substitutes based from radiosterilized human amnion or pig skin. Third-degree burns were induced in mice animal models to evaluate the effect of cellularized skin substitutes on burn wound healing. Mesenchymal phenotype was immunophenotypically confirmed by flow cytometry and cell viability was close to 100%. Skin recovery was evaluated in burned mice after seven and fourteen days post-coverage with cellularized and non-cellularized sustitutes. Histological techniques and immunofluorescence were used to evaluate re-epithelization and type I collagen depos...
Source: Cell and Tissue Banking - January 20, 2022 Category: Stem Cells Source Type: research
Repeat corneal transplantation: indication, surgical technique, and early graft failures trends in France from 2004 to 2019
ConclusionThe number of LK regrafts increased continuously, and 1/3 were performed for failure within the year. This rate increased until 2015, after which it stabilized until 2019, probably due to the better mastery of the technique. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - January 17, 2022 Category: Stem Cells Source Type: research
Advantages of decellularized bovine pericardial scaffolds compared to glutaraldehyde fixed bovine pericardial patches demonstrated in a 180-day implant ovine study
This study compared the mechanical and morphological integrity of bovine pericardium that is GA-fixated (Glycar ® patches) or decellularized (BPS), using a proprietary protocol, following implantation in an ovine model. The impact of the processing methods on tissue strength and morphology was assessed prior to implantation. Pericardial patches were then implanted in the descending aorta and main pulmonary a rtery of juvenile sheep (n = 6 per group) for 180 days, and clinically evaluated using echocardiography. At explanation, patches were evaluated for strength, calcification and biological interaction. Histology de...
Source: Cell and Tissue Banking - January 17, 2022 Category: Stem Cells Source Type: research
From 3D printing to 3D bioprinting: the material properties of polymeric material and its derived bioink for achieving tissue specific architectures
AbstractThe application of 3D printing technologies fields for biological tissues, organs, and cells in the context of medical and biotechnology applications requires a significant amount of innovation in a narrow printability range. 3D bioprinting is one such way of addressing critical design challenges in tissue engineering. In a more general sense, 3D printing has become essential in customized implant designing, faithful reproduction of microenvironmental niches, sustainable development of implants, in the capacity to address issues of effective cellular integration, and long-term stability of the cellular constructs i...
Source: Cell and Tissue Banking - January 9, 2022 Category: Stem Cells Source Type: research
Transport viable heart tissue at physiological temperature yielded higher human cardiomyocytes compared to the conventional temperature
Conclusion When needed for single-cell isolation, a heart tissue transported at 37 °C yielded higher cell density per mg tissue compared to at 4 °C, while other indicators of gene expressions for apoptosis, cardiac structural proteins, cardiac progenitor cells showed no difference. Further investigations of the isolated cells at different temperature conditions towards their pro liferation and differentiation capacities in a 3-D scaffold would be essential. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - January 7, 2022 Category: Stem Cells Source Type: research
Synergistic inhibitory effect of human umbilical cord matrix mesenchymal stem cells-conditioned medium and atorvastatin on MCF7 cancer cells viability and migration
AbstractRecent studies have demonstrated inhibitory effects of mesenchymal stem cells on breast tumors. Likewise, the emerging interest in statins as anticancer agents is based on their pleiotropic effects. In the present study, we investigated whether atorvastatin and umbilical cord matrix derived mesenchymal stem cells-conditioned medium affect the MCF7 cancer cells viability and interactions. We measured the viability of MCF7 cancer cells by MTT assay, flow cytometry, and quantitative real-time PCR. Two-dimensional culture and hanging drop aggregation assay illustrated the morphological changes. We traced the MCF7 mi...
Source: Cell and Tissue Banking - January 5, 2022 Category: Stem Cells Source Type: research
Correction to: Construction and a preliminary study of paracrine effect of bone marrow-derived endothelial progenitor cell sheet
(Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - December 6, 2021 Category: Stem Cells Source Type: research
Assessment of the efficiency of different chemical treatments and ultrasonic cleaning for defatting of cancellous bone samples
AbstractOur study aimed to asses the defatting efficiency of different methods, which are commonly used and easily available in the laboratory in order to find a method that is effective, convenient, safe, and economical. Cylindrical cancellous bone specimens were obtained from fresh-frozen human cadaver femoral condyles, c ut into multiple small specimens (Ø8 × 2 mm), and assigned to two groups that were treated with either chemical solvent soaking (Solvent group) or ultrasonic cleaning (Ultrasound group). Each group was divided into several subgroups based on different treatments. Digital photographs were take n...
Source: Cell and Tissue Banking - October 29, 2021 Category: Stem Cells Source Type: research
Characterisation of the single-cell human cardiomyocytes taken from the excess heart tissue of the right ventricular outlet in congenital heart disease
Conclusion: Cardiomyocytes taken from the wasted heart tissue might be a candidate of cardiomyocytes source to study interventions to the heart as it contained up to 13.38% cardiomyocytes, and 32.25% of cardiac progenitor cells. Moreover, perhaps when cardiac cell therapy needs autologous cardiomyocytes, less than 500 mg tissue weight can be considered as sufficie nt. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - October 28, 2021 Category: Stem Cells Source Type: research
Synergistic impact of platelet rich plasma-heparin sulfate with hydroxyapatite/zirconia on the osteoblast differentiation potential of adipose-derived mesenchymal stem cells
In conclusion, supplementation of HA/ZrO2 with PRP/HS has a synergistic impact on the ADMSCs, even in the absence of chemical induction. It seems that HA/ZrO2/PRP/HS scaffold provides a higher osteoconductive microenvironment for stem cell differentiation to osteoblasts. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - October 19, 2021 Category: Stem Cells Source Type: research
Blood and bone marrow donor registry of Istanbul medical faculty activity and experience in past 3 years
AbstractAllogeneic stem cell transplantation (SCT) offers a potential cure for some hematological malignancies. For those patients without a family donor, unrelated donor (MUD) registries serve for identifying the best donor. In the present study, we aimed to give a cross-sectional report of our registry's activity and experience as the first established national MUD registry in the country. The study is retrospective and covers the period of 2016 to 2019. A total of 1855 donor searches were performed, and 642 were included in the study. All data were electronically obtained from the institutional database system. All SCTs...
Source: Cell and Tissue Banking - October 16, 2021 Category: Stem Cells Source Type: research
