Adipose tissue-derived Muse cells promote autophagy and oxidative stress tolerance in human epidermal melanocytes
AbstractTo investigate the effect of human adipose tissue-derived multilineage-differentiating stress-enduring (Muse) cells on the oxidative stress injury of human epidermal melanocytes (HEMs) in vitro. HEMs were treated with H2O2 to establish an oxidative stress injury model and then were co-cultured with adipose tissue-derived Muse cells. Immunohistochemistry, flow cytometry and Western blotting were used to assess changes in autophagy flux, apoptosis, expression of melanin synthesis related proteins and proliferation of melanocytes. Our findings demonstrate that co-culture with Muse cells significantly increased the tol...
Source: Cell and Tissue Banking - August 20, 2022 Category: Stem Cells Source Type: research
Transdifferentiation of periodontal ligament stem cells into acinar cells using an indirect co-culture system
This study was conducted to evaluate the feasibility of differentiation of PDLSCs into salivary acinar cells by a co-culture system. PDLSCs were isolated from adult human PDL tissue and co-cultured with rat parotid ACs using an indirect co-culture system. The transdifferentiation of PDLSCs was evaluated by PCR of Aquaporin 5 (AQP5) and Carbonic anhydrase 6 (CA6) genes, then quantitative real-time PCR was used to measure the gene expression levels. The data were analyzed by ANOVA. Specific bond with the correct size on 6% acrylamide gel and TBE5X buffer showed the expression of AQP5 and CA6 in PDLSCs co-cultured with acinar...
Source: Cell and Tissue Banking - August 18, 2022 Category: Stem Cells Source Type: research
Work-related problems faced by coordinators of organ, cell, and tissue transplantations in Poland and possible ways of ameliorating them
AbstractIn recent years in Poland, the numbers of reported potential cadaveric donors of organs, tissues, and cells, and the numbers of transplantations being carried out seem to be low in the context of the size of the country population and thepresumed consent legal principle which rules transplantations. This research project was carried out on 109 Polish transplant coordinators by means of a questionnaire created specifically for this study. The goal of the project was to detect problems specific to transplant coordinators working in Poland which, when properly addressed, might improve the efficacy of transplantation n...
Source: Cell and Tissue Banking - August 12, 2022 Category: Stem Cells Source Type: research
Comparative study of decellularization techniques to obtain natural extracellular matrix scaffolds of human peripheral-nerve allografts
ConclusionsSonication can be a complementary method to decellularization of peripheral nerve allografts with sonication increasing the effectiveness of detergent ‐based protocols for the removal of unwanted cellular components from peripheral nerve allografts. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - August 12, 2022 Category: Stem Cells Source Type: research
Functional survey of decellularized tissues transplantation for infertile females
This study aimed to investigate research on decellularized tissues and transplanted into the female reproductive system. In this study, just tissues that, after transplantation, have the proper function for fertility were investigated. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - August 12, 2022 Category: Stem Cells Source Type: research
Insulin-like growth factor binding protein 5 accelerate the senescence of periodontal ligament stem cells
In conclusion, IGFBP5 can accelerate the senescence of PDLSCs, indicating the potential target for maintaining the"young state" of stem cells. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - August 8, 2022 Category: Stem Cells Source Type: research
Processing methods for human amniotic membrane as scaffold for tissue engineering with mesenchymal stromal human cells
AbstractTissue engineering is an interdisciplinary field that applies the principles of engineering and life sciences toward the development of biological substitutes that restore, maintain, or improve tissue function. The aims of this work were to compare chemically and physically processed human Amniotic Membranes (hAM) and analyze the cytocompatibility and proliferation rate (PR) of two primary human mesenchymal stromal cell lines, from different sources and donor conditions seeded over these scaffolds. The evaluated hAM processes were: cold shock to obtain a frozen amniotic membrane (FEAM) with remaining dead epithelia...
Source: Cell and Tissue Banking - July 29, 2022 Category: Stem Cells Source Type: research
Optimization of bovine embryonic fibroblast feeder layer prepared by Mitomycin C
In conclusion, the bEF-feeder prepared with 14 μg/mL Mitomycin C for 3 h or 16 μg/mL Mitomycin C for 3 h could effectively maintains the growth of bESCs, and bEF-feeder is more suitable for bESCs culture than the MEF-feeder. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - July 28, 2022 Category: Stem Cells Source Type: research
How to establish infrastructures to achieve more efficient regenerative medicine?
AbstractThe field of regenerative medicine (RM) as an innovative technology has the ability to affect the healthcare system. It develops a variety of techniques through stem cell biology, genetics, bioengineering, biomaterial science, and tissue engineering to replace or restore the role of lost, disabled, or aging cells in the human body. However, the field's proficiency has still been underwhelming at the clinical trial level. This could be due to the innovation of such technologies, as well as their incredible nature. Therefore, managing the infrastructure framework for the safe and efficient application of the aforemen...
Source: Cell and Tissue Banking - July 24, 2022 Category: Stem Cells Source Type: research
Biochemical aspects of effects of mesenchymal stem cell treatment in chronic wounds progressive healing
AbstractChronic wounds are a persistent burden for medical professionals. Despite developments and advancements in treatment, these wounds do not heal completely. Mesenchymal stem cells (MSCs) are the epicenter of regenerative medicine that have shown promising results in chronic wound regeneration. Autologous peripheral blood-derived MSCs (PB-MSCs) are comparatively new in wound healing treatment, bone-marrow-derived MSCs (BM-MSCs), and adipose-derived stem cells (ADSCs) are commonly being practiced. In the present study, PB-MSCs treatment was given to chronic wound patients. Various biochemical parameters like random blo...
Source: Cell and Tissue Banking - July 18, 2022 Category: Stem Cells Source Type: research
Effects of demineralization mode and particle size of allogeneic bone powder on its physical and chemical properties
AbstractAt present, the commonly used allogeneic bone powder in the clinic can be divided into nondemineralized bone matrix and demineralized bone matrix (DBM). Commonly used demineralizers include acids and ethylene diamine tetraacetic acid (EDTA). There may be some diversities between them. Also, the size of the bone particle can affects its cell compatibility and osteogenic ability. We produced different particle sizes i.e., < 75, 75–100, 100–315, 315–450, 450–650, and 650-1000 μm, and treated in three ways (nondemineralized, demineralized by EDTA, and demineralized by HCl). Scanning electron microsco...
Source: Cell and Tissue Banking - July 13, 2022 Category: Stem Cells Source Type: research
Preparation of laser microporous porcine acellular dermal matrix and observation of wound transplantation
This study demonstrated that the novel PADM prepared by sequential combined decellularization of trypsin, neutral protease and SDS solution method and new laser microporous technique was effective and safe in animal transplantation. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - July 9, 2022 Category: Stem Cells Source Type: research
The long non-coding RNA SNHG1 attenuates chondrocyte apoptosis and inflammation via the miR-195/IKK- α axis
This study aimed to explore how SNHG1 regulates chondrocyte apoptosis and inflammation. Our data showed that H2O2-treated chondrocytes exhibited lower expression of SNHG1 and secreted higher levels of IL-6, IL-8, and TNF- α than untreated cells. Further, overexpressing SNHG1 reduced chondrocyte apoptosis and production of inflammatory factors. Additionally, SNHG1 targets miR-195 directly, and IKK-α has direct biding sites for miR-195. Of note, IKK-α acts as an inhibitor of the NF-κB signaling pathway. These findi ngs suggest that SNHG1 can upregulate IKK-α by inhibiting miR-195 and thus, inhibit NF-κB activity. Our i...
Source: Cell and Tissue Banking - July 7, 2022 Category: Stem Cells Source Type: research
Ex-Vivo and In-Vivo Expansion of Spermatogonial Stem Cells Using Cell-Seeded Microfluidic Testis Scaffolds and Animal Model
ConclusionEx-vivo SSC differentiation and proliferation using cell-seeded microfluidic testis scaffolds maybe effective for treatment of the azoospermia. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - July 6, 2022 Category: Stem Cells Source Type: research
Removal of collagen three-dimensional scaffold bubbles utilizing a vacuum suction technique
AbstractThe process of generating type I/II collagen scaffolds is fraught with bubble formation, which can interfere with the three-dimensional structure of the scaffold. Herein, we applied low-temperature vacuum freeze-drying to remove mixed air bubbles under negative pressure. Type I and II rubber sponges were acid-solubilized via acid lysis and enzymolysis. Thereafter, vacuum negative pressure was applied to remove bubbles, and the cover glass press method was applied to shape the type I/II original scaffold. Vacuum negative pressure was applied for a second time to remove any residual bubbles. Subsequent application of...
Source: Cell and Tissue Banking - July 6, 2022 Category: Stem Cells Source Type: research
