Beneficial effects of Aucubin on restoration of rabbits with cartilage defect
In conclusion, Aucubin was beneficial for maintaining chondrocyte viability and normal morphology, and inhibiting inflammatory occurrence in rabbit osteochondral grafts preserved in vitro, and facilitated osteochondral repair and regeneration of rabbits with knee defect. These findings might provide novel insights for preservation of grafts for clinical articular cartilage repair and plastic surgery. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - April 6, 2022 Category: Stem Cells Source Type: research
The use of biological membranes for correction of congenital malformations
AbstractMany congenital malformations often require a multidisciplinary and multistep surgical treatment, including the use of biological membranes. Aims of the study were to describe the use of these membranes for the correction of malformations, their clinical performance at follow-up, and patient's tolerance to them. The study included patients treated between 2009 and November 2020 in two referral centers. They were affected by abdominal wall defects (AWD), esophageal atresia/tracheo-esophageal fistula (EA/TEF), diaphragmatic hernia (CDH), spinal defects (SD), and anorectal malformations (ARM). The human origin membran...
Source: Cell and Tissue Banking - April 6, 2022 Category: Stem Cells Source Type: research
The urgent need to achieve an optimal strategic stock of human allogeneic skin graft materials in case of a mass disaster in Poland
This study involved an analysis of selected mass disasters. From this an estimate was made from a verified casualty profile of the necessary minimum stock of human allogeneic skin graft materials. An insufficient amount of skin results from an inadequate number of skin donors, which in turn results from the current tissue donation system. Therefore, a proposal has been made for the organizational, legal and systemic changes required to improve the situation in Polish transplantology, with particular emphasis on skin donation. In order to achieve a strategic stock of human skin grafts, a tissue collecting transplantation te...
Source: Cell and Tissue Banking - March 30, 2022 Category: Stem Cells Source Type: research
Cryopreservation of human amniotic membrane for ocular surface reconstruction: a comparison between protocols
ConclusionsSwitching from liquid nitrogen cryopreservation to −80 °C would reduce manipulation, simplify the procedure, making it also cheaper. The use of dextran-based freezing medium or no medium at all (dry condition) would avoid the potential toxicity of the dimethyl sulfoxide-based freezing media. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - March 25, 2022 Category: Stem Cells Source Type: research
Circular gap forming device and two-dimensional area calculation for in vitro cell migration study
AbstractCell wound healing assay is an important experimental technique for the detection of cell migration in vitro. At present, scratch on monolayer cells using a pipette tip is commonly used. However, it is difficult to guarantee the scratch with same width, and only the migration distance of a certain part is calculated. Therefore, the experimental method needs to be optimized. ATRA was used to treat hepa1-6 mouse hepatoma cells. Circular wound with diameter of 0.2 cm were formed by a circular gap forming device. The whole cell wound region could be captured under the microscope to observe cell migration. There are a...
Source: Cell and Tissue Banking - March 22, 2022 Category: Stem Cells Source Type: research
Evaluation of anatomical and histological characteristics of human peripheral nerves: as an effort to develop an efficient allogeneic nerve graft
AbstractManagement of peripheral nerve defects is a complicated problem in clinical contexts. Autologous nerve grafting, a gold standard for surgical treatment, has been well known to have several limitations, such as donor site morbidity, a limited amount of available donor tissue, and size mismatches. Acellular nerve allografts (ANAs) have been developed as an alternative and have been applied clinically with favorable outcomes. However, because of the limited availability of commercialized ANAs due to supplier-related issues and high costs, efforts continue to produce alternative sources for ANAs. The present study eval...
Source: Cell and Tissue Banking - March 11, 2022 Category: Stem Cells Source Type: research
Effect of gamma rays and accelerated electron beam on medullary lipids decomposition: influence of dose and irradiation temperature
AbstractIonizing radiation sterilization of non-defatted bone grafts has been found to deteriorate their quality and biocompatibility due to induction of lipid peroxidation products toxic for osteoblast-like cells. Therefore, the purpose of our study was to evaluate the effect of two types of ionizing radiation —gamma rays (G) or accelerated electron beam (EB) applied with two doses at different temperature conditions on hydrocarbons production, resulting from decomposition of palmitic and oleic acids—most abundant fatty acids in medullary lipids. Bone marrow samples isolated from femoral shafts of 6 m ale donors (aged...
Source: Cell and Tissue Banking - March 10, 2022 Category: Stem Cells Source Type: research
Impact of Sars-CoV-2 pandemic on the Veneto Region multitissue bank activity
AbstractCovid pandemic affected donation activities worldwide, especially for living donation due to the lack of elective surgery. Moreover, the number of heart-beating and non-heart beating donors has recorded a decrease. Fondazione Banca dei Tessuti di Treviso (FBTV) is a non-profit healthcare organisation, located in Veneto Region, tasked with procurement, processing, preserving, validating and distributing human tissue for clinical use. During Covid-19 outbreak, operations in FBTV have never stopped and a great effort was required to maintain a standard trend of activity. The aim of this study was to describe the impac...
Source: Cell and Tissue Banking - March 2, 2022 Category: Stem Cells Source Type: research
Establishment of pediatric developmental dysplasia of the hip biobank: Shanghai children ’s hospital experience
AbstractDevelopmental dysplasia of the hip (DDH) is a debilitating condition that affects 1 –7% of newborns. Children with DDH, not treated early and effectively, will easily lead to disability. A better understanding of the biology of DDH is critical to the development of prognostic biomarkers and novel therapies. The purpose of this study was to establish a biobank of DDH genetic resou rces, to facilitate clinical and basic scientific research. The biological specimen and clinical data of DDH were collected in Shanghai Children’s Hospital from 2014 to 2021. The collection of blood samples was performed at definitive ...
Source: Cell and Tissue Banking - February 25, 2022 Category: Stem Cells Source Type: research
Comparing the effects of Elaegnus Angustifolia, Hypericum Perforatum and Psidium Guajava extracts on metabolic activity of dental pulp-derived mesenchymal stem cells
AbstractDental pulp derived-mesenchymal stem cells (DP-MSCs) is considered a suitable are candidate for tissue engineering techniques and osseous reconstruction. Based on the hypothesis thatHypericum perforatum,Elaeagnus Angustifolia andPsidium guajava extracts can be used in cell-based bone tissue engineering due to meagre cytotoxicity response in the cell culture medium, their effects on the viability and metabolic activity of DP-MSCs were investigated and compared with each extract. DP-MSCs were extracted from human dental pulp, characterized by flow cytometry, and differentiated into Osteogenic and adipogenic lineages ...
Source: Cell and Tissue Banking - February 18, 2022 Category: Stem Cells Source Type: research
Overexpression of sonic hedgehog enhances the osteogenesis in rat ectomesenchymal stem cells
AbstractEctoderm-derived mesenchymal stem cells (EMSCs) were used as potential seed cells for bone tissue engineering to treat bone defects due to their capability of rapid proliferation and osteogenic differentiation. Sonic hedgehog (Shh) signaling was reported to play an important role in the development of bone tissue, but its role is not understood. The present study investigated the role of Shh molecule in osteogenic differentiation of rat EMSCs in vitro. Rat EMSCs were isolated form nasal respiratory mucosa and identified with immunofluorescence and analyzed with other methods, including reverse transcriptase polymer...
Source: Cell and Tissue Banking - February 11, 2022 Category: Stem Cells Source Type: research
Ten-year experience with cryopreserved vascular allografts in the Croatian Cardiovascular Tissue Bank
AbstractThe Croatian Cardiovascular Tissue Bank (CTB) was established in June 2011. Activities managed by CTB are processing of heart valves and blood vessels, as well as quality control, storage, medical release and distribution of allografts. The aim of this report is to present CTB's vascular tissue activities and retrospectively evaluate the outcomes of their use in the University Hospital Centre Zagreb. Between June 2011 and July 2021, 90 vascular allografts (VAs) from 55 donors after brain death were referred to CTB. Only 54% of VAs met the tissue quality requirements while 46% of tissues were discarded. The most fre...
Source: Cell and Tissue Banking - February 7, 2022 Category: Stem Cells Source Type: research
Preservation of corneal stromal lenticule: review
AbstractCorneal stromal lenticule is a part of corneal stroma, which can be created by manual dissection, by femtosecond laser from the donor cornea, but chiefly it is a waste product of a refractive procedure ReLex SMILE (Small Incision Lenticule Extraction). Corneal lenticule has a huge potential in corneal surgery. In recent years, many studies have been published to show the possibility to use this tissue to treat corneal defects, as well as in refractive surgery. Thanks to the quantity of lenticules which arise every day during SMILE operations, this tissue is much more accessible than any other kind of corneal tiss...
Source: Cell and Tissue Banking - February 6, 2022 Category: Stem Cells Source Type: research
Tissue engineering of human ear pinna
AbstractAuricular deformities (Microtia) can cause physical, social as well as psychological impacts on a patient ’s wellbeing. Biofabrication of a complex structure such as ear pinna is not precise with currently available techniques. These limitations can be overcome with the help of tissue engineering. In this article, the authors presented molding and three dimensional (3D) printing to generate a flexible , human size ear pinna. The decellularization of goat ear cartilage protocol and bioink alkaline digestion protocol was followed to yield complete removal of all cellular components without changing the properties o...
Source: Cell and Tissue Banking - February 1, 2022 Category: Stem Cells Source Type: research
Evaluation the ability of acellular ovine small intestine submucosa to load and release of mineral pitch and its anti-inflammatory effects
In this study, the ability of the acellular ovine small intestine submucosa (AOSIS) to load and release of Mineral Pitch (MP) was first investigated, and it was found that the preparation of the scaffold by a modified method enables it to load and release water-soluble drugs. Then, 32 male Wistar rats were divided into four groups, a third-degree burn was created, and except for the control group, the others were treated with: AOSIS, WJ-MSCs seeded AOSIS, or AOSIS loaded with WJ-MSCs and MP. Wound sampling on the 5th day after treatment showed that the number of intact and degranulated mast cells in the treatment groups wa...
Source: Cell and Tissue Banking - January 27, 2022 Category: Stem Cells Source Type: research
