Retraction Note: Investigation of the mechanism of gamma irradiation effect on bovine bone
(Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - August 24, 2023 Category: Stem Cells Source Type: research
Effect of gamma and Ultraviolet-C sterilization on BMP-7 level of indigenously prepared demineralized freeze-dried bone allograft
This study aimed to compare and evaluate
the effectiveness of Gamma and Ultraviolet-C rays in sterilizing indigenously
prepared DFDBA and assess their effect on the quantity of BMP-7 presen t in the
allograft. DFDBA samples from non-irradiated, gamma irradiated, and UV-C
irradiated groups were tested for BMP-7 level and samples sterilized with gamma
and UV-C rays were analysed for sterility testing. The estimated mean
BMP-7 level was highest in non-irradiated D FDBA samples, followed by UV-C
irradiated, and the lowest in gamma irradiated samples. Our study concluded
that UV-C rays effectively steri...
Source: Cell and Tissue Banking - August 14, 2023 Category: Stem Cells Source Type: research
Effect of storage media on chondrocyte viability during cold storage of osteochondral dowels
In this study, we compared three tissue/cell media (DMEM/F12, RPMI-1640 and X-VIVO 10) for their ability to maintain chondrocyte viability during hypothermic storage for 28 days. Porcine osteochondral dowels were stored in each media for 28 days and cell viability was assessed every 7 days. Over the 28 day storage period, the chondrocyte viability of dowels stored in DMEM/F12, RPMI-1640, and X-VIVO 10 media all declined in a similar fashion. Our results show that all three media were equivalent in their ability to maintain cell viability of the cartilage tissue and provides rationale for the use of lower cost cell med...
Source: Cell and Tissue Banking - August 8, 2023 Category: Stem Cells Source Type: research
Evaluation of bone marrow-derived mesenchymal stem cells with eggshell membrane for full-thickness wound healing in a rabbit model
AbstractBiomaterials capable of managing wounds should have essential features like providing a natural microenvironment for wound healing and as support material for stimulating tissue growth. Eggshell membrane (ESM) is a highly produced global waste due to increased egg consumption. The unique and fascinating properties of ESM allow their potential application in tissue regeneration. The wound healing capacity of bone marrow-derived mesenchymal stem cells (BM-MSCs), ESM, and their combination in rabbits with full-thickness skin defect (2 × 2 cm2) was evaluated. Twenty-five clinically healthy New Zealand White rabb...
Source: Cell and Tissue Banking - August 4, 2023 Category: Stem Cells Source Type: research
Platelet-rich plasma alleviates knee arthritis in rats by inhibiting p65
In this study, we explored the potential therapeutic effects of platelet-rich plasma (PRP) and identified molecular targets for treating KOA. A rat model of KOA was established via the Hulth method and primary knee joint chondrocytes were isolated to evaluate the effects of PRP and shRNA targeting p65 (sh-p65). ELISA was used to detect inflammatory factors, including IL-6, IL-1 β, and TNF-α. HE staining, Safranin O/Fast Green staining and Masson staining were performed to evaluate the morphology of articular cartilage, followed by detection of p65, COL2A1, ACAN, MMP13, and ADAMTS5 expression. The proliferation and apopto...
Source: Cell and Tissue Banking - July 27, 2023 Category: Stem Cells Source Type: research
Activated platelet-rich plasma accelerate endometrial regeneration and improve pregnancy outcomes in murine model of disturbed endometrium
AbstractPlatelet Rich Plasma (PRP) contains high concentrations of growth factors, therefore, PRP activation results in their release, stimulating the process of healing and regeneration. The study was conducted to check whether activated platelet-rich plasma (aPRP) treatment can improve regeneration of the endometrium in an experimental model of ethanol-induced disturbed endometrium. Seventy-two female Wistar rats were randomly assigned into the control group, disturbed endometrium (DE) group and aPRP treated group. Activation of PRP was performed by adding thrombin. All the animals were sacrificed on day 1, da y 3, day...
Source: Cell and Tissue Banking - July 19, 2023 Category: Stem Cells Source Type: research
Culture of cerebrospinal fluid-contacting neurons from neonatal mouse spinal cord
AbstractCerebrospinal fluid-contacting neurons (CSF-cNs) act crucial role in chemosensory and mechanosensory function in spinal cord. Recently, CSF-cNs were found to be an immature neuron and may be involved in spinal cord injury recovery. But how to culture it and explore its function in vitro are not reported in previous research. Here, we first reported culture and identification of CSF-cNs in vitro. We first established a protocol for in vitro culture of CSF-cNs from the cervical spinal cord of mice within 24 h after birth. Polycystic kidney disease 2-like 1 (PKD2L1)+ cells were isolated by fluorescence-activated cel...
Source: Cell and Tissue Banking - June 27, 2023 Category: Stem Cells Source Type: research
The role of oxygen tension in cell fate and regenerative medicine: implications of hypoxia/hyperoxia and free radicals
AbstractOxygen pressure plays an integral role in regulating various aspects of cellular biology. Cell metabolism, proliferation, morphology, senescence, metastasis, and angiogenesis are some instances that are affected by different tensions of oxygen. Hyperoxia or high oxygen concentration, enforces the production of reactive oxygen species (ROS) that disturbs physiological homeostasis, and consequently, in the absence of antioxidants, cells and tissues are directed to an undesired fate. On the other side, hypoxia or low oxygen concentration, impacts cell metabolism and fate strongly through inducing changes in the expres...
Source: Cell and Tissue Banking - June 26, 2023 Category: Stem Cells Source Type: research
The trend of allogeneic tendon decellularization: literature review
AbstractTendon injuries repair is a significant burden for orthopaedic surgeons. Finding a proper graft material to repair tendon is one of the main challenges in orthopaedics, for which the requirement of substitute for tendon repair would be different for each clinical application. Among biological scaffolds, the use of decellularized tendon increasingly represents an interesting approach to treat tendon injuries and several articles have investigated the approaches of tendon decellularization. To understand the outcomes of the the approaches of tendon decellularization on effect of tendon transplantation, a literature r...
Source: Cell and Tissue Banking - June 24, 2023 Category: Stem Cells Source Type: research
Comparison of human acellular amniotic membranes with acellular amniotic membranes pretreated with MPLA for repair of fascia in rats
This study compared the effectiveness of amniotic membrane and amniotic membrane pretreated with monophosphoryl lipid A (MPLA) for repairing the fascia of the abdomen with tension sutures. A total of 24 rats were randomly divided into three groups of eight each. In the control group, the fascia was repaired only with non-absorbable monofilament yarn under tension. In the second and third groups, in addition to the control treatment, acellular amniotic membrane was used and MPLA pretreated amniotic membrane was used, respectively. At 3, 7 and 14 days after treatment, the fascia repair was examined macroscopically and micros...
Source: Cell and Tissue Banking - May 25, 2023 Category: Stem Cells Source Type: research
Characterization of an advanced viable bone allograft with preserved native bone-forming cells
AbstractBone grafts are widely used to successfully restore structure and function to patients with a broad range of musculoskeletal ailments and bone defects. Autogenous bone grafts are historically preferred because they theoretically contain the three essential components of bone healing (ie, osteoconductivity, osteoinductivity, and osteogenicity), but they have inherent limitations. Allograft bone derived from deceased human donors is one alternative that is also capable of providing both an osteoconductive scaffold and osteoinductive potential but, until recently, lacked the osteogenic component of bone healing. Relat...
Source: Cell and Tissue Banking - May 25, 2023 Category: Stem Cells Source Type: research
Graft dislocation following Descemet stripping automated endothelial keratoplasty: a comparative cohort analysis of Optisol GS and organ culture preserved corneas
AbstractPrevious studies have suggested risk factors for graft dislocation after Descemet stripping automated endothelial keratoplasty (DSAEK). We aimed to investigate the influence of eye bank storage solution as a risk factor for graft dislocation, as this could have significant implications for eye banking practices. This retrospective analysis compared cohorts from the QEII Health Sciences Center, Halifax, Canada (donor corneas preserved in Optisol GS) and the Royal Victorian Eye and Ear Hospital, Melbourne, Australia (donor corneas preserved in organ culture). Patient, surgical, and post-operative data were collected ...
Source: Cell and Tissue Banking - May 25, 2023 Category: Stem Cells Source Type: research
Effects of glucose on the proliferation of human umbilical cord blood hematopoietic stem cells
This study demonstrates the relationship between glucose and the activation of the NLRP3 inflammasome. Based on these results, glucose causes the expansion of CD34+HSCs through its effect on HSCs in simultaneous culture. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - May 25, 2023 Category: Stem Cells Source Type: research
Inter-placental variability is not a major factor affecting the healing efficiency of amniotic membrane when used for treating chronic non-healing wounds
This study aimed to evaluate the efficacy of cryopreserved amniotic membrane (AM) grafts in chronic wound healing, including the mean percentage of wound closure per one AM application, and to determine whether the healing efficiency differs between AM grafts obtained from different placentas. A retrospective study analyzing inter-placental differences in healing capacity and mean wound closure after the application of 96 AM grafts prepared from nine placentas. Only the placentas from which the AM grafts were applied to patients suffering from long-lasting non-healing wounds successfully healed by AM treatment were include...
Source: Cell and Tissue Banking - May 25, 2023 Category: Stem Cells Source Type: research
Effect of hypoxia on the expression of microRNA in extracellular vesicles of human umbilical cord stem cells in vitro
In conclusion, this study demonstrated for the first time that microRNA expression in extracellular vesicles of human umbilical vein stem cells cultured under hypoxia is different from that under normal conditions, and these microRNAs may be markers for detecting hypoxia. (Source: Cell and Tissue Banking)
Source: Cell and Tissue Banking - May 24, 2023 Category: Stem Cells Source Type: research
