Implementation of PD-L1 22C3 IHC pharmDx < sup > TM < /sup > in Cell Block Preparations of Lung Cancer: Concordance with Surgical Resections and Technical Validation of CytoLyt ® Prefixation
Conclusions: PD-L1 22C3 IHC pharmDxTM shows good reliability if used with CB preparations. CF does not impact assay results significantly. Clinical validation with outcome data is needed, and digital methods of assessment should be further investigated.Acta Cytologica (Source: Acta Cytologica)
Source: Acta Cytologica - June 29, 2020 Category: Cytology Source Type: research
Nuclear Morphology and the Biology of Cancer Cells
Background: For more than a century, diagnostic pathologists have used morphologic abnormalities of the nucleus as essential diagnostic features to distinguish benign from malignant cells. These features include nuclear enlargement and increased nuclear-to-cytoplasmic ratio, nuclear membrane irregularities, hyperchromasia, and abnormal chromatin distribution. As our knowledge about the genetic and epigenetic abnormalities of cancer cells has increased in recent decades, the pathophysiologic mechanisms that underlie these morphologic abnormalities remain incompletely understood.Summary: This review attempts to summarize bio...
Source: Acta Cytologica - June 22, 2020 Category: Cytology Source Type: research
Comparison of ThinPrep Integrated Imager-Assisted Screening versus Manual Screening of ThinPrep Liquid-Based Cytology Specimens
Conclusion: The Imager arm was confirmed to have an equivalent and non-inferior capacity in the detection of cervical lesions compared with the Manual arm, suggesting that its practical application in cervical cytology tests is highly possible.Acta Cytologica (Source: Acta Cytologica)
Source: Acta Cytologica - June 12, 2020 Category: Cytology Source Type: research
Fine-Needle Aspiration Cytology Is an Effective Diagnostic Tool in Paediatric Patients with Mucoepidermoid Carcinoma as Secondary Neoplasm
This study was designed to define clinical and instrumental findings and morphological features of MEC on fine-needle aspiration cytology (FNAC) samples in paediatric patients with and without a previous neoplasm. Five patients under 19 years are included in this series. FNAC was performed in all patients on a parotid nodule. We have identified 2 groups of patients: (a) 2 cases with previous history of malignancy (acute lymphoblastic leukaemia and Hodgkin lymphoma) and (b) 3 cases without previous malignant neoplasms. In all cases, a final diagnosis of MEC was rendered.Key Messages: MEC may occur as a second malignancy in ...
Source: Acta Cytologica - June 11, 2020 Category: Cytology Source Type: research
Axillary Nodal Metastasis with Papillary Morphology: An Uncommon Origin
Conclusion: Nodal metastases with papillary cytomorphology can rarely arise from nonbreast primaries. The presence of papillary pattern, particularly in the absence of a clinically detectable breast lesion, should raise the possibility of a metastasis. Correlation with patient history, imaging findings and judicious use of IHC studies are crucial for a correct diagnosis.Acta Cytologica (Source: Acta Cytologica)
Source: Acta Cytologica - June 11, 2020 Category: Cytology Source Type: research
Combined Analysis of Intragastric Malignant Exfoliation and Ca 72.4 Concentration in Stomach Adenocarcinoma: The “GL1 Ca 72.4” Parameter
Conclusions: The combined cyto-molecular parameter “GL1 Ca 72.4+” appears to be a strong indicator of aggressive tumor behavior and a significant prognostic factor of poor survival for GC patients.Acta Cytologica (Source: Acta Cytologica)
Source: Acta Cytologica - June 11, 2020 Category: Cytology Source Type: research
Pleural Effusion in Pulmonary and Extrapulmonary Blastomycosis
Conclusion: Pleural effusion detected by imaging is common in blastomycosis patients. Blastomycosis can involve pleural fluid in both pulmonary and extrapulmonary diseases. A broad infectious differential that includes blastomycosis should be considered to make a timely diagnosis and initiate antifungal therapy to prevent systemic infection and further dissemination of the disease.Acta Cytologica (Source: Acta Cytologica)
Source: Acta Cytologica - July 2, 2019 Category: Cytology Source Type: research
Liquid Biopsy: A New Tool in Oncology
Acta Cytologica 2019;63:1 (Source: Acta Cytologica)
Source: Acta Cytologica - July 2, 2019 Category: Cytology Source Type: research
Utility of Squash Cytology in Spinal Lesions with Special Reference to Ki67 Immunostain
Conclusion: Squash smear cytology is a rapid intraoperative technique for diagnosing spinal lesions, with high specificity and high positive predictive value. It is more effective in diagnosing neoplasms than non-neoplastic lesions. Ki67 immunostaining can be done on cytology smears to effectively differentiate between WHO grade I and grade II spinal tumours.Acta Cytologica (Source: Acta Cytologica)
Source: Acta Cytologica - June 24, 2019 Category: Cytology Source Type: research
Announcement: The International System for Reporting Serous Fluid Cytopathology
Serous fluids are a common but important specimen type in a cytopathology laboratory. There is as yet no agreed standardized terminology to allow uniformity in reporting on these specimens. Given that serous fluids are a rich source of cytopathological as well as molecular information on a range of benign and often advanced malignant conditions, a unified approach to handling and reporting these specimens covering the pre-analytical, analytical and postanalytical stages seems timely. Representatives of the international cytology community have come together once again to develop an algorithmic diagnostic and management app...
Source: Acta Cytologica - June 24, 2019 Category: Cytology Source Type: research
Characterizing the Effect of Automated Cell Sorting Solutions on Cytomorphological Changes
Conclusion: We showed that cells swell in the solution after 1 h of storage and contract as time progresses. Together, our findings have important implications for how mathematical analysis is applied during the automated screening process.Acta Cytologica (Source: Acta Cytologica)
Source: Acta Cytologica - June 24, 2019 Category: Cytology Source Type: research
Cytodiagnostic Sensitivity of Fine Needle Aspiration Biopsy for Hodgkin ’s Lymphoma Is Decreased in Patients with Human Immunodeficiency Virus Infection
Conclusion: The diagnostic sensitivity of FNA biopsy was significantly attenuated in the HIV+ cohort. In HIV-infected patients presenting with lymphadenopathy, increased clinical suspicion of HL is critical to avoid misdiagnosis.Acta Cytologica (Source: Acta Cytologica)
Source: Acta Cytologica - June 24, 2019 Category: Cytology Source Type: research
Genomic Alterations in Undifferentiated Malignant Tumors with Rhabdoid Phenotype and Loss of BRG1 Immunoexpression Identified by Fine Needle Aspirates
Conclusions: BRG1 deficiency reflects underlying mutation inSMARCA4 gene in some but not all cases, suggesting that additional mechanisms may be causing BRG1 silencing. Pathogenic mutations in TP53 in all tumors are consistent with their highly aggressive nature. Recognizing the cytomorphology of this group of neoplasms and confirming their BRG1-deficient status by immunocytochemistry not only has prognostic implications, but may also impart potentially therapeutic value in the near future.Acta Cytologica (Source: Acta Cytologica)
Source: Acta Cytologica - June 21, 2019 Category: Cytology Source Type: research
Author Index / Subject Index
Acta Cytologica 2019;63:347 (Source: Acta Cytologica)
Source: Acta Cytologica - June 21, 2019 Category: Cytology Source Type: research
Pelims
Acta Cytologica 2019;63:251 –254 (Source: Acta Cytologica)
Source: Acta Cytologica - June 21, 2019 Category: Cytology Source Type: research
