Cell Block Optimization: A Comparative Study of Quality Variables in 4 Different Cell Block Methods
Conclusions: TCM was the most robust CB technique, retaining high scores for ranking of quantity and morphology in a variety of specimen cellularities and specimen types.Acta Cytologica (Source: Acta Cytologica)
Source: Acta Cytologica - June 2, 2021 Category: Cytology Source Type: research
Nationwide Rereview of Normal Cervical Cytologies before High-Grade Cervical Lesions or before Invasive Cervical Cancer
Sweden has experienced an unexpected #x3e;30% increase in cervical cancer incidence among women with normal cytological screening results. We therefore performed a nationwide assessment of false-negative cytology before invasive cervical cancer. The Swedish national cervical screening registry identified 2,150 normal cytologies taken up to 10 years before 903 cases of invasive cervical cancer. The 27 cytological laboratories in Sweden were asked to rereview the slides, and all of them completed the rereview. One thousand nine hundred fifteen slides were retrieved and reviewed. Abnormalities were found in 30% of the slides,...
Source: Acta Cytologica - June 2, 2021 Category: Cytology Source Type: research
A Preliminary Study of Deep-Learning Algorithm for Analyzing Multiplex Immunofluorescence Biomarkers in Body Fluid Cytology Specimens
Conclusion: MFI combined with DLA provides a potential tool to assist in cytological diagnosis of metastatic malignancy in body fluid samples. Larger studies are warranted to test the clinical validity of the approach.Acta Cytologica (Source: Acta Cytologica)
Source: Acta Cytologica - June 2, 2021 Category: Cytology Source Type: research
DNA Recovery Using Ethanol-Based Liquid Medium from FTA Card-Stored Samples for HPV Detection
Conclusions: The HPV-DNA detection positivity rates were 70.6% for the samples collected directly in liquid medium and 64.7% for the samples stored on the FTA card, with high detection accuracy in the DNA samples recovered from the FTA card (area under the curve = 0.958; 95% confidence interval = 0.890 –1.000). The concordance between the results obtained using the 2 storage media was 94.1% (Kappa = 0.866). These preliminary results suggest that collection of cervical material on an FTA card may be an alternative to storage in liquid medium since the liquid medium has some limitations. In additi on, DNA recovery from the...
Source: Acta Cytologica - May 5, 2021 Category: Cytology Source Type: research
Digital/Computational Technology for Molecular Cytology Testing: A Short Technical Note with Literature Review
This article describes our attempt to apply AI technology to cytologic digital images. For molecular analysis, cytologic materials, such as smear, LBC, and cell blocks, have been successfully used for targeted single gene detection and multiplex gene analysis with next-generation sequencing. As a future perspective, the system can be connected to full automation by combining digital cytopathology with AI application to detect target cancer cells and to perform molecular analysis. The literature review is updated according to the subjects.Acta Cytologica (Source: Acta Cytologica)
Source: Acta Cytologica - April 30, 2021 Category: Cytology Source Type: research
Accuracy of Breast Fine-Needle Aspiration Biopsy Using the International Academy of Cytology Yokohama System in Clinico-Radiologically Indeterminate Lesions: Initial Findings Demonstrating Value in Lesions of Low Suspicion of Malignancy
Conclusion: The study reconfirms the excellent accuracy of breast FNAB using the IAC Yokohama system in diagnosing breast malignancies. Furthermore, BIRADS 4a lesions found to be belonging to the cytological benign category may be excluded from CRB and kept on clinical follow-up.Acta Cytologica (Source: Acta Cytologica)
Source: Acta Cytologica - April 27, 2021 Category: Cytology Source Type: research
Diagnostic Utility of LEF1 Immunostain in Cytology Specimens of Solid Pseudopapillary Neoplasm of Pancreas
Conclusion: LEF1 shows 100% sensitivity and specificity in cytology specimens for SPN and can be valuable immuno stain in the diagnosis of SPN in cytology cell blocks.Acta Cytologica (Source: Acta Cytologica)
Source: Acta Cytologica - April 22, 2021 Category: Cytology Source Type: research
Recommendations of the European Advisory Committee of Cytotechnology and European Federation of Cytology Societies for Training and Education of Cytotechnologists in Europe
Background: Faced with changes in cytodiagnostics, cervical cancer screening programs, the introduction and application of new methods, the cytotechnological educational program requires the necessary changes and additions. Insufficient, uneven as well as inaccessible education of cytotechnologists in European countries was the basis for making these recommendations.Summary: The results of previous research and publications related to the currently available education of cytotechnologists in Europe, the needs and suggestions were given by the European Advisory Committee of Cytotechnology (EACC) and European Federation of C...
Source: Acta Cytologica - April 15, 2021 Category: Cytology Source Type: research
MOC31 Immunostaining in the Diagnosis of Metastatic Adenocarcinoma in Serous Fluid: Special Emphasis on Atypical Cytological Cases
Conclusion: MOC31 is an excellent marker of metastatic adenocarcinoma in the serous effusion. The membranous positivity of MOC31 and negative calretinin immuno staining are helpful in atypical cytological cases to avoid the diagnostic dilemma. The MOC31 positivity is significantly useful in discrete atypical cells which are more challenging to recognize.Acta Cytologica (Source: Acta Cytologica)
Source: Acta Cytologica - April 7, 2021 Category: Cytology Source Type: research
The Role of Cell Blocks and Immunohistochemistry in Thyroid Atypia of Undetermined Significance/Follicular Lesion of Undetermined Significance Bethesda Category
Conclusion: In conclusion, CB cellularity is essential for successful immunohistochemistry application and further diagnostic workup of AUS/FLUS cases.Acta Cytologica (Source: Acta Cytologica)
Source: Acta Cytologica - March 31, 2021 Category: Cytology Source Type: research
Cytologic Features of Lipogranulomatous Reaction in an Axillary Sentinel Lymph Node
Conclusion: Lipogranulomas may appear at axillary sentinel lymph nodes because of fat necrosis induced by previous breast biopsy. The most important consideration is not confounding MGC with epithelial cell clusters. This can occur with not well-processed samples, especially if unmounted.Acta Cytologica (Source: Acta Cytologica)
Source: Acta Cytologica - March 11, 2021 Category: Cytology Source Type: research
Ultrasound-Guided Minimally Invasive Autopsy of Respiratory Muscles as a Safe and Cost-Effective Technique in COVID-19 Pandemic Era
Acta Cytologica (Source: Acta Cytologica)
Source: Acta Cytologica - March 9, 2021 Category: Cytology Source Type: research
Morphological Differences between Liquid-Based Cytology and Conventional Preparation in Endometrial Endometrioid Carcinoma Grade 1 and Grade 3, and the Differentiation of Grades in Each Method
Conclusion: In the endometrial cytology, it became clear that the cell image was different between LBC and CP and between G1 and G3. By microscopic examination understanding the characteristics of the cell image in LBC, endometrial LBC could be useful to diagnose endometrial carcinoma.Acta Cytologica (Source: Acta Cytologica)
Source: Acta Cytologica - March 2, 2021 Category: Cytology Source Type: research
Using Disposable Membrane Cell Collector to Enrich Lung Adenocarcinoma Cells in Bloody Pleural Effusion for Anaplastic Lymphoma Kinase Fusion Gene Detection
Conclusions: Using the disposable membrane cell collector to process BPE of lung adenocarcinoma patients for RNA extraction and ALK gene detection is more effective and successful compared with the traditional methods, and it is suggested to be further applied and popularized in clinical practice.Acta Cytologica (Source: Acta Cytologica)
Source: Acta Cytologica - February 25, 2021 Category: Cytology Source Type: research
Applications of Computational Pathology in Head and Neck Cytopathology
Background: The application of computational technology to head and neck cytology material has been explored experimentally in several areas with a variety of potential applications.Summary: This review summarizes the application of these techniques to the diagnosis of thyroid, salivary gland, and other head and neck fine-needle aspiration specimens. Current limitations and potential future applications in diagnosis are discussed along with the possibilities for therapeutic applications of computational methodology.Key Message: Particularly promising applications include resolving diagnostic uncertainty in indeterminate th...
Source: Acta Cytologica - February 23, 2021 Category: Cytology Source Type: research
