The impact of sitagliptin in palmitic acid-induced insulin resistance in human HepG2 cells through the suppressor of cytokine signaling 3/phosphoinositide 3-kinase/protein kinase B pathway
In this study, we established an in vitro insulin resistance cell model for human HepG2 cells to investigate the possible mechanism of the effect of sitagliptin on glucose metabolism via the SOCS3/phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway. Since insulin resistance first develops in the liver, palmitic acid was used to generate an insulin resistance cell model in human HepG2 cells, after which small interfering ribonucleic acid (siRNA)-SOCS3 and sitagliptin were used to intervene. We then examined the changes in cell viability and biochemical indices in the insulin resistance cell model. SOCS3, Akt, an...
Source: J Physiol Pharmacol - July 15, 2023 Category: Drugs & Pharmacology Authors: R Ma L Quan Q-Q-G Aleteng L Li J Zhu S Jiang Source Type: research
The impact of sitagliptin in palmitic acid-induced insulin resistance in human HepG2 cells through the suppressor of cytokine signaling 3/phosphoinositide 3-kinase/protein kinase B pathway
In this study, we established an in vitro insulin resistance cell model for human HepG2 cells to investigate the possible mechanism of the effect of sitagliptin on glucose metabolism via the SOCS3/phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway. Since insulin resistance first develops in the liver, palmitic acid was used to generate an insulin resistance cell model in human HepG2 cells, after which small interfering ribonucleic acid (siRNA)-SOCS3 and sitagliptin were used to intervene. We then examined the changes in cell viability and biochemical indices in the insulin resistance cell model. SOCS3, Akt, an...
Source: Journal of Physiology and Pharmacology - July 15, 2023 Category: Drugs & Pharmacology Authors: R Ma L Quan Q-Q-G Aleteng L Li J Zhu S Jiang Source Type: research
The impact of sitagliptin in palmitic acid-induced insulin resistance in human HepG2 cells through the suppressor of cytokine signaling 3/phosphoinositide 3-kinase/protein kinase B pathway
In this study, we established an in vitro insulin resistance cell model for human HepG2 cells to investigate the possible mechanism of the effect of sitagliptin on glucose metabolism via the SOCS3/phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt) pathway. Since insulin resistance first develops in the liver, palmitic acid was used to generate an insulin resistance cell model in human HepG2 cells, after which small interfering ribonucleic acid (siRNA)-SOCS3 and sitagliptin were used to intervene. We then examined the changes in cell viability and biochemical indices in the insulin resistance cell model. SOCS3, Akt, an...
Source: J Physiol Pharmacol - July 15, 2023 Category: Drugs & Pharmacology Authors: R Ma L Quan Q-Q-G Aleteng L Li J Zhu S Jiang Source Type: research
A novel combination of sitagliptin and melatonin ameliorates T2D manifestations: studies on experimental diabetic models
ConclusionThe combination treatment is superior to monotherapies in the management of T2D. (Source: Journal of Endocrinological Investigation)
Source: Journal of Endocrinological Investigation - July 14, 2023 Category: Endocrinology Source Type: research
Network pharmacology, molecular docking, and molecular dynamics simulation to elucidate the mechanism of anti-aging action of Tinospora cordifolia
In conclusion, this study used network pharmacology, molecular docking, and MD simulation to identify the compounds in T. cordifolia and proposed a potential mechanism for anti-aging activity. These results suggest future directions for the prevention and treatment of age-related diseases.PMID:37439907 | DOI:10.1007/s11030-023-10684-w (Source: Atherosclerosis)
Source: Atherosclerosis - July 13, 2023 Category: Cardiology Authors: Amisha Bisht Disha Tewari Sanjay Kumar Subhash Chandra Source Type: research
Effect of sitagliptin on down-regulation of KAT7 and SIRT1 gene expression in breast cancer cell line MCF7
CONCLUSIONS: These results suggest that sitagliptin has effects on the histone epigenetic landscape. This topic deserves further study due to the current sample use of DPP4 inhibitors in diabetic patients.PMID:37433152 | DOI:10.24875/CIRU.22000189 (Source: Cirugia y Cirujanos)
Source: Cirugia y Cirujanos - July 11, 2023 Category: Surgery Authors: Tanya C Ruteaga-Navarro Miguel A Reyes-Romero Quitzia L Torres-Salazar Source Type: research
Effect of sitagliptin on down-regulation of KAT7 and SIRT1 gene expression in breast cancer cell line MCF7
CONCLUSIONS: These results suggest that sitagliptin has effects on the histone epigenetic landscape. This topic deserves further study due to the current sample use of DPP4 inhibitors in diabetic patients.PMID:37433152 | DOI:10.24875/CIRU.22000189 (Source: Cirugia y Cirujanos)
Source: Cirugia y Cirujanos - July 11, 2023 Category: Surgery Authors: Tanya C Ruteaga-Navarro Miguel A Reyes-Romero Quitzia L Torres-Salazar Source Type: research
Mechanism of preventive effects of exendin-4 and des-fluoro-sitagliptin in a murine model of fructose-induced prediabetes
Biochim Biophys Acta Mol Cell Biol Lipids. 2023 Jul 8:159363. doi: 10.1016/j.bbalip.2023.159363. Online ahead of print.ABSTRACTProtective effects of exendin-4 (glucagon-like peptide-1 -GLP-1- receptor agonist) and des-fluoro-sitagliptin (dipeptidyl peptidase-4 inhibitor) on fructose-induced hepatic disturbances were evaluated in prediabetic rats. Complementary, a possible direct effect of exendin-4 in human hepatoblastoma-derived cell line HepG2 incubated with fructose in presence/absence of exendin-9-39 (GLP-1 receptor antagonist) was investigated. In vivo, after 21 days of fructose rich diet, we determined: glycemia, ins...
Source: Biochimica et Biophysica Acta - July 10, 2023 Category: Biochemistry Authors: Mar ía Cecilia Castro Hern án Gonzalo Villagarcía Guillermo Schinella Mar ía Laura Massa Flavio Francini Source Type: research
Mechanism of preventive effects of exendin-4 and des-fluoro-sitagliptin in a murine model of fructose-induced prediabetes
Biochim Biophys Acta Mol Cell Biol Lipids. 2023 Jul 8:159363. doi: 10.1016/j.bbalip.2023.159363. Online ahead of print.ABSTRACTProtective effects of exendin-4 (glucagon-like peptide-1 -GLP-1- receptor agonist) and des-fluoro-sitagliptin (dipeptidyl peptidase-4 inhibitor) on fructose-induced hepatic disturbances were evaluated in prediabetic rats. Complementary, a possible direct effect of exendin-4 in human hepatoblastoma-derived cell line HepG2 incubated with fructose in presence/absence of exendin-9-39 (GLP-1 receptor antagonist) was investigated. In vivo, after 21 days of fructose rich diet, we determined: glycemia, ins...
Source: Mol Biol Cell - July 10, 2023 Category: Molecular Biology Authors: Mar ía Cecilia Castro Hern án Gonzalo Villagarcía Guillermo Schinella Mar ía Laura Massa Flavio Francini Source Type: research
Mechanism of preventive effects of exendin-4 and des-fluoro-sitagliptin in a murine model of fructose-induced prediabetes
Biochim Biophys Acta Mol Cell Biol Lipids. 2023 Jul 8:159363. doi: 10.1016/j.bbalip.2023.159363. Online ahead of print.ABSTRACTProtective effects of exendin-4 (glucagon-like peptide-1 -GLP-1- receptor agonist) and des-fluoro-sitagliptin (dipeptidyl peptidase-4 inhibitor) on fructose-induced hepatic disturbances were evaluated in prediabetic rats. Complementary, a possible direct effect of exendin-4 in human hepatoblastoma-derived cell line HepG2 incubated with fructose in presence/absence of exendin-9-39 (GLP-1 receptor antagonist) was investigated. In vivo, after 21 days of fructose rich diet, we determined: glycemia, ins...
Source: Molecular Medicine - July 10, 2023 Category: Molecular Biology Authors: Mar ía Cecilia Castro Hern án Gonzalo Villagarcía Guillermo Schinella Mar ía Laura Massa Flavio Francini Source Type: research
Mechanism of preventive effects of exendin-4 and des-fluoro-sitagliptin in a murine model of fructose-induced prediabetes
Biochim Biophys Acta Mol Cell Biol Lipids. 2023 Jul 8:159363. doi: 10.1016/j.bbalip.2023.159363. Online ahead of print.ABSTRACTProtective effects of exendin-4 (glucagon-like peptide-1 -GLP-1- receptor agonist) and des-fluoro-sitagliptin (dipeptidyl peptidase-4 inhibitor) on fructose-induced hepatic disturbances were evaluated in prediabetic rats. Complementary, a possible direct effect of exendin-4 in human hepatoblastoma-derived cell line HepG2 incubated with fructose in presence/absence of exendin-9-39 (GLP-1 receptor antagonist) was investigated. In vivo, after 21 days of fructose rich diet, we determined: glycemia, ins...
Source: Biochimica et Biophysica Acta - July 10, 2023 Category: Biochemistry Authors: Mar ía Cecilia Castro Hern án Gonzalo Villagarcía Guillermo Schinella Mar ía Laura Massa Flavio Francini Source Type: research
Mechanism of preventive effects of exendin-4 and des-fluoro-sitagliptin in a murine model of fructose-induced prediabetes
Biochim Biophys Acta Mol Cell Biol Lipids. 2023 Jul 8:159363. doi: 10.1016/j.bbalip.2023.159363. Online ahead of print.ABSTRACTProtective effects of exendin-4 (glucagon-like peptide-1 -GLP-1- receptor agonist) and des-fluoro-sitagliptin (dipeptidyl peptidase-4 inhibitor) on fructose-induced hepatic disturbances were evaluated in prediabetic rats. Complementary, a possible direct effect of exendin-4 in human hepatoblastoma-derived cell line HepG2 incubated with fructose in presence/absence of exendin-9-39 (GLP-1 receptor antagonist) was investigated. In vivo, after 21 days of fructose rich diet, we determined: glycemia, ins...
Source: Mol Biol Cell - July 10, 2023 Category: Molecular Biology Authors: Mar ía Cecilia Castro Hern án Gonzalo Villagarcía Guillermo Schinella Mar ía Laura Massa Flavio Francini Source Type: research
Controlling Pharmaceutical Costs in a Student-Run Free Clinic in a Resource-Limited Patient Setting
In this study, we aimed to analyze the financial impact of these measures on the clinic.A query of clinic data over the past 5 years identified 299 active PDAPs, corresponding to 299 fully-subsidized prescriptions. In 2017, there were 35 active PDAPs, increasing to 52 (2018), 62 (2019), and 82 (2020) before a decline to 68 PDAPs in 2021. The company affiliated with the most PDAPs varied annually: GlaxoSmithKline (2017), Lilly (2018, 2019, 2020), and both GlaxoSmithKline and Lilly (2021). The most frequent medications were sitagliptin (2017), insulin (2018, 2019), albuterol (2017, 2018), and dulaglutide (2020, 2021).In addi...
Source: Journal of Community Health - July 5, 2023 Category: International Medicine & Public Health Source Type: research
Targeting DPP4-RBD interactions by sitagliptin and linagliptin delivers a potential host-directed therapy against pan-SARS-CoV-2 infections
Int J Biol Macromol. 2023 Jun 27:125444. doi: 10.1016/j.ijbiomac.2023.125444. Online ahead of print.ABSTRACTHighly mutated SARS-CoV-2 is known aetiological factor for COVID-19. Here, we have demonstrated that the receptor binding domain (RBD) of the spike protein can interact with human dipeptidyl peptidase 4 (DPP4) to facilitate virus entry, in addition to the usual route of ACE2-RBD binding. Significant number of residues of RBD makes hydrogen bonds and hydrophobic interactions with α/β-hydrolase domain of DPP4. With this observation, we created a strategy to combat COVID-19 by circumventing the catalytic activity of D...
Source: International Journal of Biological Macromolecules - June 29, 2023 Category: Biochemistry Authors: Shailendra Mani Anupamjeet Kaur Kamini Jakhar Geetika Kumari Sudipta Sonar Amit Kumar Sudesna Das Santosh Kumar Vijay Kumar Rakesh Kundu Anil Kumar Pandey Umesh Prasad Singh Tanmay Majumdar Source Type: research
Targeting DPP4-RBD interactions by sitagliptin and linagliptin delivers a potential host-directed therapy against pan-SARS-CoV-2 infections
Int J Biol Macromol. 2023 Jun 27;245:125444. doi: 10.1016/j.ijbiomac.2023.125444. Online ahead of print.ABSTRACTHighly mutated SARS-CoV-2 is known aetiological factor for COVID-19. Here, we have demonstrated that the receptor binding domain (RBD) of the spike protein can interact with human dipeptidyl peptidase 4 (DPP4) to facilitate virus entry, in addition to the usual route of ACE2-RBD binding. Significant number of residues of RBD makes hydrogen bonds and hydrophobic interactions with α/β-hydrolase domain of DPP4. With this observation, we created a strategy to combat COVID-19 by circumventing the catalytic activity ...
Source: International Journal of Biological Macromolecules - June 29, 2023 Category: Biochemistry Authors: Shailendra Mani Anupamjeet Kaur Kamini Jakhar Geetika Kumari Sudipta Sonar Amit Kumar Sudesna Das Santosh Kumar Vijay Kumar Rakesh Kundu Anil Kumar Pandey Umesh Prasad Singh Tanmay Majumdar Source Type: research
