The expression of the renin-angiotensin-aldosterone system in the skin and its effects on skin physiology and pathophysiology.
Authors: Aleksiejczuk M, Gromotowicz-Poplawska A, Marcinczyk N, Przylipiak A, Chabielska E Abstract The local renin-angiotensin-aldosterone system (RAAS) is fully expressed in the human skin at the mRNA and protein level. Local RAAS is known to play a regulatory function in epidermal proliferation, wound healing, scarring, cutaneous heating adaptation, and aging. There are also some indications of its role in the regulation of hair growth and sebum secretion. Impaired wound healing, skin diseases associated with diabetes, cancer development, psoriasis, and scleroderma may be related to changes in skin RAAS activity...
Source: Journal of Physiology and Pharmacology - September 22, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

The impact of metalloestrogens on the physiology of male reproductive health as a current problem of the XXI century.
Authors: Jurkowska K, Kratz EM, Sawicka E, Piwowar A Abstract In the XXIst century in highly developed countries, progressively decreasing male reproductive potential is indicated. In recent years epidemiological studies indicate the deterioration of semen parameters: reduction of ejaculate volume, sperm count, and mobility, as well as abnormalities in their morphology. Male infertility can result from many different agents, such as: anatomical or genetic abnormalities, systemic or neurological diseases, infections, trauma, iatrogenic injury, gonadotoxins and development of sperm antibodies and lifestyle (especiall...
Source: Journal of Physiology and Pharmacology - September 22, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Prognostic usefulness of serum myostatin in advanced chronic liver disease: its relation to gender and correlation with inflammatory status.
Authors: Skladany L, Koller T, Molcan P, Vnencakova J, Zilincan M, Jancekova D, Kukla M Abstract In hospitalized patients with advanced chronic liver disease (ACLD), we aimed to evaluate the association between myostatin and muscle mass, its relation to inflammation and to assess the added prognostic value of myostatin for survival. In a prospective single-center cohort study, inclusion criteria were: consecutive hospitalization for ACLD and Child-Pugh score ≥ 7 points. Baseline parameters were myostatin, C-reactive protein (CRP), hand-grip strength (HGS), mid-arm muscle circumference (MAC), transversal psoas mu...
Source: Journal of Physiology and Pharmacology - September 22, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

The current proteomic landscape of the porcine liver.
Authors: Ozgo M, Lepczynski A, Robak P, Herosimczyk A, Marynowska M Abstract The main objective of the study was to create a reproducible protein map of the liver in healthy growing piglets. The analysis was performed on liver homogenates obtained from 8 castrated male piglets (PIC x Penerlan P76) at the 50 days. Two-dimensional electrophoresis and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry allowed to determine the proteomic profile of the liver. Liver proteins were separated at pH 4 - 7, followed by 12% SDS-PAGE. As a result, 470 ± 44 spots were present on the 2...
Source: Journal of Physiology and Pharmacology - September 22, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Diverse inhibition of forkhead box O1 activity by linoleic acid isomers - potential role in lipid metabolism in HepG2 cells and livers of C57BL/6J mice.
Authors: Siwiec E, Szynkowska A, Milkiewicz M, Marlicz W, Stachowska E Abstract Conjugated dienes of linoleic acid (CLA) are constitutional and geometric isomers of linoleic acid that are commonly used as dietary supplements during body mass reduction. Their role in the reduction of lipid deposits in liver tissue is not unequivocal. CLA contain an equimolar mixture of two isomers of linoleic acid: trans-10,cis-12 CLA and cis-9,trans-11. Only one isomer - trans-10,cis-12 CLA exhibits fat-reducing properties, cis-9,trans-11 CLA does not. The main goal of this study was to determine if CLA isomers affect the activatio...
Source: Journal of Physiology and Pharmacology - September 22, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Serum endocan level in diabetes mellitus of patients with cirrhosis and risk of subsequent development of spontaneous bacterial peritonitis.
Authors: Zuwala-Jagiello J, Pazgan-Simon M, Simon K, Kukla M, Murawska-Cialowicz E, Grzebyk E Abstract The presence of type 2 diabetes mellitus (DM) in patients with cirrhosis is associated with an increased risk of spontaneous bacterial peritonitis (SBP) which may represent an increased susceptibility to infections. Endocan is a key player in the regulation of inflammatory disorders, and a biomarker in bacteremia and sepsis. To investigate the association between both endocan and DM, and developing SBP, we conducted a retrospective cohort study. Three hundred and thirty patients (179 men, 151 women; mean age 61.0 ...
Source: Journal of Physiology and Pharmacology - September 22, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Maternal high-fat diet during pregnancy and lactation provokes depressive-like behavior and influences the irisin/brain-derived neurotrophic factor axis and inflammatory factors in male and female offspring in rats.
Authors: Gawlinska K, Gawlinski D, Przegalinski E, Filip M Abstract A balanced maternal diet is necessary for the proper health and development of offspring. Recent clinical and preclinical studies have strongly indicated that maternal exposure to a high-fat diet (HFD) can have an irreversible impact on the structure and function of the offspring's brain and affect the immune system, which may predispose the offspring to brain disorders, including depression. The irisin/brain-derived neurotrophic factor (BDNF) axis is a pathway that influences several neurobehavioral mechanisms involved in the pathogenesis of menta...
Source: Journal of Physiology and Pharmacology - September 22, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Predictability of the duration of motor blockade induced by unique injection of intrathecal prilocaine - an observational study.
Authors: Lacroix SP, Malaise PR, Degey SV, Deflandre EP Abstract Prilocaine is widely used for spinal anesthesia. Its intermediate effect makes it a valuable choice for one-day surgery. The duration of the motor blockade (DMB) may have an impact on the length of stay. The goal of this study was to establish a correlation between the DMB and different parameters (hyperbaric prilocaine dose, puncture level, surgical position, age, patient weight, and patient height). We prospectively enrolled adult patients scheduled for ambulatory surgery (n = 384). Univariate and multivariate regressions (backward stepwise) were ap...
Source: Journal of Physiology and Pharmacology - September 22, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Effect of acute exercise on mRNA and protein expression of main components of the lipolytic complex in different skeletal muscle types in the rat.
Authors: Miklosz A, Baranowski M, Lukaszuk B, Zabielski P, Chabowski A, Gorski J Abstract Adipose triglyceride lipase (ATGL) hydrolyses the first bond of triacylglycerols. The activity of the enzyme is elevated by comparative gene identification 58 (CGI-58), and reduced by G0/G1 switch gene 2 (G0S2) protein. There are no data on the effect of acute exercise on the behavior of particular components of the lipolytic complex in different skeletal muscle types, therefore, the aim of the present study was to examine that topic. The experiments were carried out on four groups of male Wistar rats: 1) control 2) rats runni...
Source: Journal of Physiology and Pharmacology - September 22, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Different nutrient intake and prevalence of gastrointestinal comorbidities in women with endometriosis.
Authors: Schink M, Konturek PC, Herbert SL, Renner SP, Burghaus S, Blum S, Fasching PA, Neurath MF, Zopf Y Abstract Even though endometriosis presents one of the most common gynaecological diseases, the pathogenesis is insufficiently studied. Besides immunologic, inflammatory or oxidative processes, recent studies also suggest an influence of nutrition on disease onset and progression. Because data about the actual nutrient intake of endometriosis patients are scarce, we aimed to examine the actual nutrient intake and potential influencing factors in these women. A total of 156 women with endometriosis (EM) and 52 ...
Source: Journal of Physiology and Pharmacology - August 24, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Evaluation of the effect of liposomes loaded with chlorogenic acid in treatment of 2,4,6-trinitrobenzenesulfonic acid-induced murine colitis.
Authors: Krajewska JB, Pietruszka P, Tomczyk D, Chen C, Owczarek A, Karolewicz B, Czapor-Irzabek H, Gorniak A, Fichna J Abstract Crohn's Disease (CD), one of the types of inflammatory bowel disease, poses a significant challenge to modern healthcare. This condition severely impacts patients' quality of life, and its incidence is continuously rising. Despite constant research, current treatment options are limited and largely unsuccessful and result in serious side effects, therefore new therapy alternatives are needed. Liposomal formulation provides a new hope for disease management. In our study, we characterized ...
Source: Journal of Physiology and Pharmacology - August 24, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Omentin, vaspin and irisin in chronic liver diseases.
Authors: Waluga M, Kukla M, Kotulski R, Zorniak M, Boryczka G, Kajor M, Ciupinska-Kajor M, Lekstan A, Olczyk P, Waluga E Abstract Omentin and vaspin are adipokines potentially considered in the development of liver pathology. Irisin is new myokin potentially participating in energy processes in the organisms. The aim of this study was to evaluate the plasma concentration of these cytokines and the relationships of them with selected parameters of laboratory tests and of histopathological changes in selected chronic liver diseases: non-alcoholic fatty liver diseases (NAFLD), primary biliary cholangitis (PBC) and alc...
Source: Journal of Physiology and Pharmacology - August 24, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Inhibitory effects of herbal decoction Ojeoksan on proliferation and migration in vascular smooth muscle cells.
Authors: Han BH, Yoon JJ, Kim HY, Ahn YM, Jin SN, Wen JF, Lee HS, Lee YJ, Kang DG Abstract The proliferation of vascular smooth muscle cells plays a crucial role in pathogenesis of cardiovascular disease. The principal objective of this study was to determine the effects of Ojeoksan (OJS) on human aortic smooth muscle cell (HASMC) proliferation induced by tumor necrosis factor α (TNF-aα). Thymidine incorporation after TNF-α treatment was increased and this effect was inhibited significantly by OJS treatment. HASMC proliferation and migration by kinetic live cell imaging were also reduced by treatm...
Source: Journal of Physiology and Pharmacology - August 24, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

The role of perivascular adipose tissue and endogenous hydrogen sulfide in vasoactive responses of isolated mesenteric arteries in normotensive and spontaneously hypertensive rats.
Authors: Cacanyiova S, Majzunova M, Golas S, Berenyiova A Abstract Perivascular adipose tissue (PVAT) and hydrogen sulfide (H2S) play important roles in the modulation of vasoactive responses and can interfere with the ethiopathogenesis of essential hypertension. The aim of this study was to evaluate the mutual relationship between PVAT and H2S (endogenously produced, exogenous) in vasoactive responses of isolated mesenteric arteries (MA) in adult normotensive (Wistar) and spontaneously hypertensive rats (SHR). In SHR, hypertension was associated with cardiac hypertrophy and increased contractility; however, there ...
Source: Journal of Physiology and Pharmacology - August 24, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Hawthorn revisited: time- and dose-dependent cardioprotective action of WS-1442 special extract in the reperfusion-induced arrhythmia model in rats in vivo.
Authors: Zorniak M, Porc MP, Krzeminski TF Abstract Standardized WS-1442 extract from Crataegus oxycantha (hawthorn) leaves and berries is one of the most widely studied preparations received from hawthorn. This popular substance is known from its positive influence on the cardiovascular system. The current research aimed to evaluate the optimal dose of standardized WS-1442 extract and the most beneficial period for its use. The study analysis was based on experiments previously conducted on male Sprague-Dawley rats (n = 152). The animals were divided into subgroups to examine the relationship between the dose-depe...
Source: Journal of Physiology and Pharmacology - August 24, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Urocortin 2 in patients with hypertension treated with angiotensin converting enzyme inhibitors or angiotensin receptor blockers.
We examined a group of 65 patients with primary hypertension receiving at least 3 antihypertensive drugs. In all of them plasma level of Ucn2, anthropometric measurements, biochemical tests, ambulatory blood pressure monitoring (ABPM), and echocardiography were performed. There were no differences in Ucn2 level related to beta-blockers, calcium channel blockers or diuretics, but we observed that in patients treated with angiotensin converting enzyme inhibitors (ACEI) (n = 52) serum Ucn2 levels were significantly higher than in patients treated with angiotensin-receptor blockers (ARBs) (n = 13) (10.93 versus 5.56 ng/mL; P
Source: Journal of Physiology and Pharmacology - August 24, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Evaluation of platelet reactivity during combined antiplatelet therapy in patients with stable coronary artery disease in relation to diabetes type 2 and the GPIIB/IIIA receptor gene polymorphism.
Authors: Jastrzebska M, Lisman D, Szelepajlo A, Oledzki S, Chelstowski K, Clark JS, Siennicka A Abstract Antiplatelet therapy resistance against acetylsalicylic acid (ASA) and/or clopidogrel in coronary heart disease (CHD) is common with diabetes mellitus. One factor might involve platelet receptor ITGB3 gene polymorphism. We aimed to assess resistance together with platelet reactivity parameters, the polymorphism, plus diabetes type 2 coexistence. The study included 185 patients with CHD, including 58 diabetics, aged 62.3 ± 8.2 years. Patients were treated long-term with ASA, plus clopidogrel, both 75 mg/d....
Source: Journal of Physiology and Pharmacology - July 31, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Nitric oxide as a modulator in platelet- and endothelium-dependent antithrombotic effect of eplerenone in diabetic rats.
Authors: Gromotowicz-Poplawska A, Kloza M, Aleksiejczuk M, Marcinczyk N, Szemraj J, Kozlowska H, Chabielska E Abstract We have recently demonstrated the antithrombotic effect of eplerenone on the arterial thrombotic process in diabetic rats associated with suppression of coagulation and enhancement of fibrinolysis. The aim of this study was to evaluate the role of platelets and endothelium in the mechanism of eplerenone antithrombotic action. Diabetes was induced in male Wistar rats with a single injection of streptozotocin (65 mg/kg). On the 25th day, treatment with eplerenone (100 mg/kg) was initiated for 10 days...
Source: Journal of Physiology and Pharmacology - July 31, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Age-related changes in the local intestinal renin-angiotensin system in normotensive and spontaneously hypertensive rats.
Authors: Pasanen L, Launonen H, Siltari A, Korpela R, Vapaatalo H, Salmenkari H, Forsgard RA Abstract Local renin-angiotensin systems (RAS) are found in many tissues. The main physiological effects of RAS are driven by the balance between two pathways: the angiotensin-converting enzyme I - angiotensin II receptor type 1 (ACE1-AT1R) axis and the angiotensin-converting enzyme 2 - Mas-receptor (ACE2-MAS) axis. The local intestinal RAS functions both as a paracrine regulator and as a regulator of inflammation. The expression of local RAS is known to change with age in many tissues, but age-related changes in the intest...
Source: Journal of Physiology and Pharmacology - July 31, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

The importance of selected markers of inflammation and blood-brain barrier damage for short-term ischemic stroke prognosis.
Authors: Lasek-Bal A, Jedrzejowska-Szypulka H, Student S, Warsz-Wianecka A, Zareba K, Puz P, Bal W, Pawletko K, Lewin-Kowalik J Abstract Acute cerebral ischemia triggers local and systemic immune response. The aims of this project was to assess the blood serum concentration of the markers of inflammation and markers of the blood brain barrier damage on the first day of ischemic stroke, and the mutual correlations between these marker levels. Patients with first-in-life stroke were analysed according to: plasma concentration of the following markers on the first day of stroke: interleukin 2 (IL-2) and interleuki 6 (...
Source: Journal of Physiology and Pharmacology - July 31, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Arginine and tetrahydrobiopterin supplementation in rats with salt-induced blood pressure increase: minor hypotensive effect but improvement of renal haemodynamics.
Authors: Kuczeriszka M, Walkowska A, Olszynski KH, Rafalowska J, Sadowski J, Kompanowska-Jezierska E Abstract High salt (HS) intake can lead to hypertension, probably the result of the predominance of vasoconstrictor reactive oxygen species over vasodilator nitric oxide (NO). We aimed to examine if the supposed NO deficiency and the resultant blood pressure increase could be corrected by supplementation of L-arginine, the substrate, and tetrahydrobiopterin (BH4), a co-factor of NO synthases. Wistar rats without known genetic background of salt sensitivity were exposed to HS diet (4%Na) for 10 or 26 days, without or...
Source: Journal of Physiology and Pharmacology - July 31, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

The urine podocin/creatinine ratio as a novel biomarker of cardiorenal syndrome in dogs due to degenerative mitral valve disease.
Authors: Szczepankiewicz B, Paslawska U, Paslawski R, Gebarowski T, Zasada W, Michalek M, Noszczyk-Nowak A Abstract Dysfunction of heart leads inevitable to the dysfunction of kidney which is termed as the cardiorenal syndrome (CRS). Previous studies have confirmed existence of CRS in dogs with degenerative mitral valve disease (DMVD). The goal of the study was to assess the usefulness of commercial test to measure podocyturia in dogs and test the urine podocine/creatinine ratio (UPoC) as an early marker of kidney injury. Urine podocine/creatinine ratio was calculated because numbers of podocytes is dependent on th...
Source: Journal of Physiology and Pharmacology - July 31, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Disruption of histamine/H3 receptor signal reduces collagen deposition in cultures scar myofibroblasts.
Authors: Piera L, Olczak S, Kun T, Galdyszynska M, Ciosek J, Szymanski J, Drobnik J Abstract A suitable inflammatory signal influences extracellular matrix accumulation and determines the quality of the myocardial infarction scar. The aim of the present study was to determine the influence of mast cell sonicates or histamine on collagen accumulation in heart myofibroblast culture and on the deposition of collagen in the myocardial infarction scar. The histamine receptor involved in the process was investigated. Myocardial infarction was induced by ligation of the left coronary artery. Myofibroblasts were isolated f...
Source: Journal of Physiology and Pharmacology - July 31, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Melatonin from slow-release implants upregulates claudin-2 in the ovine choroid plexus.
This study evaluated the effect of slow-release melatonin implants on the expression of claudin-2 (CLDN2), a pore-forming protein that allows the paracellular passage (between the cells) of select inorganic cations and water, in the ovine ChP. The studies were conducted on ovariectomized, estradiol-implanted ewes during seasonal anestrus (May/June). The ewes were implanted with slow-release-melatonin implants (n = 6, Melovine 18 mg) or sham-implanted (n = 6). Blood samples were collected for melatonin and prolactin measurements. The ewes were sacrificed 40 days after the melatonin/sham implantation, and the ChPs from the b...
Source: Journal of Physiology and Pharmacology - July 31, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Exposure to air pollution and oxidative stress markers in patients with potentially malignant oral disorders.
Authors: Gregorczyk-Maga I, Celejewska-Wojcik N, Gosiewska-Pawlica D, Darczuk D, Kesek B, Maga M, Wojcik K Abstract The etiopathogenesis of potentially malignant oral disorders (PMOD) has not been fully understood yet. Recent results suggest that oxidative stress may be involved in the etiology of PMOD. Production of oxidants seems to be the major biological effect responsible for tissue injury and inflammatory response to air pollution. The aim of this study was to compare the oxidative stress markers and antioxidant potential in saliva of PMOD subjects and healthy controls in periods of high and low air pollution...
Source: Journal of Physiology and Pharmacology - June 8, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Detection of resistance to anti-tuberculosis drugs in the clinical isolates of Mycobacterium tuberculosis from Slovakia through comparison between phenotypic and genetic methods and evaluation of resistance levels with clinical parameter.
Authors: Mokry J, Porvaznik I, Kusnir P, Dohal M, Solovic I Abstract The incidence of tuberculosis (TB) in some countries increases continuously, especially caused by mycobacterial strains resistant to various anti-tuberculotic drugs (AT). The emergence and spread of drug-resistant tuberculosis (multidrug-resistant - MDR-TB, and extensively drug-resistant - XDR-TB) suggest the crucial role of pharmacotherapy protocol tailored to the respective patient with MDR-TB or XDR-TB (a personalized approach) and requirements for fast and precise diagnostics of the degree of resistance. The aim of this study was to characteri...
Source: Journal of Physiology and Pharmacology - June 8, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Serum fatty acid binding proteins as a potential biomarker in atrial fibrillation.
Authors: Golaszewska K, Harasim-Symbor E, Polak-Iwaniuk A, Chabowski A Abstract Atrial fibrillation (AF) is a commonly occurring arrhythmia which significantly reduces patients' quality of life and substantially shortens life expectancy. Although long chain fatty acids (LCFAs) are the basic energy substrates for myocardial metabolism, their excess can result in lipotoxicity, which increases the risk of arrhythmia. Intracellularly, LCFAs are bound by fatty acid biding proteins (FABPs) and this results in low level of free LCFAs in the cytoplasm. Based on this principle, FABPs are considered "safeguards" ag...
Source: Journal of Physiology and Pharmacology - June 8, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Regulatory effect of dexamethasone on tracheal calcium processing proteins and mucosal secretion.
In this study, mice were subcutaneously injected with dexamethasone for 5 days, and their tracheal samples were collected by dividing the trachea into the cervical, and thoracic sections based on its anatomical structure. The localization of TRPV4, TRPV6, CaBP-9k, and PMCA1 proteins was detected in the tracheal epithelium, submucosal glands, cartilages and muscles. Dexamethasone treatment downregulated the mRNA expression of the four calcium processing genes and mucin producing genes. The dexamethasone-induced decrease in the secretion of mucosubstances in the trachea was determined by performing Alcian blue-periodic acid-...
Source: Journal of Physiology and Pharmacology - June 8, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Eicosanoid profiling in effluent of isolated perfused heart of Tg αq*44 mice with advanced heart failure.
In conclusion, eicosanoid profiling in isolated perfused heart pointed to PGI2, PGD2 and 12-HETE as the most abundant AA metabolites of the isolated murine heart. In Tgαq*44 mice, the end-stage phase of heart failure was accompanied by major activation of cyclooxygenase pathways (PGI2, TXA2, PGD2, PGE2, PGF2α), 8,9-, 11,12-EET/DHETs pathways and 12-, 15-HETEs pathways in the heart. PMID: 31172972 [PubMed - in process] (Source: Journal of Physiology and Pharmacology)
Source: Journal of Physiology and Pharmacology - June 8, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

The implication of adipocyte ATP-binding cassette A1 and G1 transporters in metabolic complications of obesity.
Authors: Choromanska B, Mysliwiec P, Hady HR, Dadan J, Mysliwiec H, Bonda T, Chabowski A, Miklosz A Abstract Obesity is characterised by imbalance in lipid metabolism manifested by high concentrations of circulating triacylglycerols and total cholesterol as well as low high-density lipoprotein (HDL) levels. Abnormalities related to these lipids lead to metabolic complications such as type 2 diabetes, arterial hypertension and cardiovascular disease. Despite extensive research, it is still unclear why a subset of obese subjects develop metabolic syndrome, while others do not. The aim of our work was to assess total ...
Source: Journal of Physiology and Pharmacology - June 8, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Effects of vinclozolin exposure on the expression and activity of SIRT1 and SIRT6 in the porcine ovary.
Authors: Gorczyca G, Wartalski K, Tabarowski Z, Duda M Abstract Within the mammalian reproductive system sirtuin 1 and 6 (SIRT1, SIRT6) are considered to contribute to steroid hormone signaling and control of reproductive physiology. Therefore, the specific question is whether and how a commonly used dicarboximide fungicide with antiandrogenic activity, vinclozolin (Vnz) alters SIRT1 and SIRT6 expression and whether both investigated sirtuins positively affect survival of the follicles after vinclozolin exposure. Immunocytochemistry and immunohistochemistry were performed to localize SIRT1 and SIRT6 expression in c...
Source: Journal of Physiology and Pharmacology - June 8, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Corrigendum.
Authors: PMID: 31172975 [PubMed - in process] (Source: Journal of Physiology and Pharmacology)
Source: Journal of Physiology and Pharmacology - June 8, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Effect of ghrelin on the apoptosis of various cells. A critical review.
Authors: Kurowska P, Mlyczynska E, Rak A Abstract Ghrelin is a protein hormone secreted from the gastric mucosa, but is also expressed in a variety of tissues. The biological function of ghrelin is widely known, including regulation of food intake, body weight, gastrointestinal, cardiovascular, reproductive, immune functions, cell proliferation and hormone secretion. The growth hormone secretagogue receptor (GHS-R) mediates the effect of ghrelin. Depending on cell type, ghrelin either induces apoptosis or has anti-apoptotic effect. Apoptosis is a physiological process that plays an important role in many biological...
Source: Journal of Physiology and Pharmacology - April 28, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Application of ventricular tachyarrhythmia definitions of the updated Lambeth Conventions provides incompatibility with earlier results, masks antifibrillatory activity and reduces inter-observer agreement.
This study examined whether the arrhythmia definitions of LC I and LC II yield the same qualitative results and whether LC II improves inter-observer agreement. Two independent investigators performed blinded arrhythmia analysis of the electrocardiograms of isolated, Langendorff rat hearts subjected to regional ischemia and perfused with Class I antiarrhythmics with 3 or 5 mM K+ in the perfusate. Data obtained with arrhythmia definitions of LC I and LC II were compared within and between observers. Applying ventricular fibrillation (VF) definition of LC II significantly increased VF incidence and reduced VF onset time irre...
Source: Journal of Physiology and Pharmacology - April 28, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

The influence of the low-energy defibrillating impulse on the endothelin-1 concentration in the left heart ventricle and blood serum of the healthy rabbits.
This study is aimed at evaluating time-dependent changes in ET-1 levels in serum and the heart of a healthy rabbit following the application of a low-energy two-phase shock impulse. The research was conducted in 35 healthy rabbits at the age of 36 - 42 weeks and with body mass from 3200 to 4150 grams. The animals were divided in a randomized way into four groups depending on the dose of the electrical energy planned for the application during the experiment. The life parameters of the animals were monitored with the application of BeneView T5 patient monitor. The concentration of endothelin-1 in the groups was measured bef...
Source: Journal of Physiology and Pharmacology - April 28, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Viscoelastic properties of plasma fibrin clots are similar in patients on rivaroxaban and vitamin K antagonists.
Authors: Kopytek M, Zabczyk M, Natorska J, Siudut J, Malinowski KP, Ptaszek P, Glajcar A, Goralczyk T, Undas A Abstract Unfavorable fibrin clot features have been observed in patients with venous thromboembolism (VTE). We investigated whether rivaroxaban, a direct factor Xa inhibitor, and vitamin K antagonists (VKAs) can improve plasma clot viscoelastic properties. We studied four age- and sex-matched groups: 25 healthy controls, 15 VTE patients taking rivaroxaban 20 mg/day (blood concentration, 145 (67 - 217) ng/ml), 15 VTE patients taking VKA (INR: 2 - 3), and 15 VTE patients who stopped oral anticoagulant therap...
Source: Journal of Physiology and Pharmacology - April 28, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Serum galectin 3, galectin 9 and galectin 3-binding proteins in patients with active and inactive inflammatory bowel disease.
This study assessed the serum levels of galectin-3 (Gal-3) and galectin-3 (Gal-9) and galectin 3 binding protein (Gal-3BP), and evaluated their associations with the clinical characteristics and levels of inflammatory markers in patients with inflammatory bowel disease (IBD). A total of 48 patients with ulcerative colitis (UC), 77 with Crohn's disease (CD), and 30 healthy volunteers participated in the study. Complete blood counts, C-reactive protein, fibrinogen, albumin, glucose, creatinine, Gal-3, Gal-9, and Gal-3BP were measured. The median Gal-3 and Gal-9 levels did not differ between patients and controls. The median ...
Source: Journal of Physiology and Pharmacology - April 28, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Astragalosides modulates contractile function of toad gastrocnemius muscle.
Authors: Wei SS, Zou YW, Liu ZB, Meng W, Zhao K, Wang SH, Yao LH Abstract Huangqi (Radix Astragali) is a well-known traditional Chinese herbal medicine, it is an effective treatment for consumptive disease, such as the common cold, diarrhea, fatigue and cardiac diseases. Astragalosides (AST) is the main component of Huangqi. The purpose of this study is to investigate the modulation effect of AST on the skeletal muscle contractile function. Our results showed that the toad gastrocnemius muscle contractile response was significantly increased after the use of AST (25 mg/L, bath for the isolated muscle), which produc...
Source: Journal of Physiology and Pharmacology - April 28, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Relations between markers of cardiac remodelling and left ventricular collagen in an isoproterenol-induced heart damage model.
Authors: Adamcova M, Baka T, Dolezelova E, Aziriova S, Krajcirovicova K, Karesova I, Stanko P, Repova K, Simko F Abstract There is a great urgency of detecting and monitoring myocardial fibrosis in clinical practice with the aim to improve and personalize therapy against cardiac remodelling. Hence, the aim of this study was to describe alterations in and show potential correlations between the structural characteristics and the molecular and biochemical markers of cardiac remodelling on a model of isoproterenol-induced heart failure. Two groups of 3-month-old male Wistar rats (n = 8 per group) were sacrificed after...
Source: Journal of Physiology and Pharmacology - April 28, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

The place of tachykinin NK2 receptor antagonists in the treatment diarrhea-predominant irritable bowel syndrome.
Authors: Szymaszkiewicz A, Malkiewicz A, Storr M, Fichna J, Zielinska M Abstract Tachykinins act as neurotransmitters and neuromodulators in the central and peripheral nervous system. Preclinical studies and clinical trials showed that inhibition of the tachykinin receptors, mainly NK2 may constitute a novel attractive option in the treatment of irritable bowel syndrome (IBS). In this review, we focused on the role of tachykinins in physiology and pathophysiology of gastrointestinal (GI) tract. Moreover, we summed up recent data on tachykinin receptor antagonists in the therapy of IBS. Ibodutant is a novel drug wit...
Source: Journal of Physiology and Pharmacology - April 26, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Aldosterone and mineralocorticoid receptors in regulation of the cardiovascular system and pathological remodelling of the heart and arteries.
Authors: Sztechman D, Czarzasta K, Cudnoch-Jedrzejewska A, Szczepanska-Sadowska E, Zera T Abstract In the review we discuss the role of mineralocorticoid receptors (MRs) in regulation and pathological remodelling of the cardiovascular system and the therapeutic potential of pharmacological targeting of MRs in cardiovascular diseases. MRs are expressed in organs involved in cardiovascular homeostasis: brain, heart, kidneys and vessels. The excessive activation of MRs has deleterious effects on the cardiovascular system through sympatho-excitation, elevated salt appetite, and renal retention of salt with consequent p...
Source: Journal of Physiology and Pharmacology - March 23, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Influence of hypoxia prevailing in post-infarction heart on proangiogenic gene expression and biological features of human myoblast cells applied as a pro-regenerative therapeutic tool.
Authors: Zimna A, Wiernicki B, Kolanowski T, Malcher A, Rozwadowska N, Labedz W, Kubaszewski L, Kurpisz M Abstract Cardiovascular diseases along with MI (myocardial infarction) lead to regional ischaemia and hypoxic conditions, which prevail after infarction. Diminished O2 saturation which is related to elevated level of hypoxia inducible factor 1 (HIF-1) transcription factor, may switch the expression of many genes. To maximize effect of therapies proposed by regenerative medicine, it is essential to verify (within different time points after MI) the expression of proangiogenic genes and their receptors that are r...
Source: Journal of Physiology and Pharmacology - March 23, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Acrylamide toxicity and cholinergic nervous system.
Authors: Kopanska M, Muchacka R, Czech J, Batoryna M, Formicki G Abstract Acrylamide (ACR) is a chemical compound, that forms in starchy food products during cooking at high-temperatures, including frying, baking, and roasting. ACR is a known lethal neurotoxin. The presented review suggests that the mechanism of ACR's neurotoxicity may be related to an impaired cholinergic transmission in the central and peripheral nervous system and redox imbalance. These may not only affect ongoing brain functions but also participate in etiology of neurodegeneration. PMID: 30898983 [PubMed - in process] (Source: Journal of P...
Source: Journal of Physiology and Pharmacology - March 23, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Effects of controlled physical activity on immune cell phenotype in peripheral blood in prehypertension - studies in preclinical model and randomised crossover study.
Authors: Mazur M, Glodzik J, Szczepaniak P, Nosalski R, Siedlinski M, Skiba D, Rewiuk K, Salakowski A, Czesnikiewicz-Guzik M, Grodzicki T, Guzik TJ, Mikolajczyk TP Abstract Hypertension (HT) is a global public health issue. There are many behavioural risk factors including unhealthy diet, tobacco use and alcohol consumption as well physical inactivity that contribute to the development of high blood pressure (BP) and its complications. Favourable effect of regular physical activity on treatment or prevention of hypertension by improvement of endothelial function is widely accepted however little is known about its ...
Source: Journal of Physiology and Pharmacology - March 23, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Experimental autoimmune myocarditis in rats and therapeutic histamine H1 - H4 receptor inhibition.
Authors: Stasiak A, Gola J, Kraszewska K, Mussur M, Kobos J, Mazurek U, Stark H, Fogel WA Abstract Myocarditis, a life threatening disease, is still not adequately treated. Histamine plays an important role in physiology and pathophysiology of cardiovascular system. All four histamine receptors (H1R - H4R), are present in the heart. Experimental autoimmune myocarditis (EAM) was used to investigate which histamine receptor had a greater impact on the disease's progression. EAM was evoked in Lewis rats by porcine myosin immunization. Mepyramine, ranitidine and ciproxifan were used to inhibit H1R, H2R and H3R receptor...
Source: Journal of Physiology and Pharmacology - March 23, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Standarized Tribulus terrestris extract protects against rotenone-induced oxidative damage and nigral dopamine neuronal loss in mice.
Authors: Alzahrani S, Ezzat W, Elshaer RE, Abd El-Lateef AS, Mohammad HMF, Elkazaz AY, Toraih E, Zaitone SA Abstract Strong evidence proposes that brain oxidative DNA damage and microglia activation contribute to Parkinson's disease (PD) pathogenesis. Traditional therapeutic regimens for PD can only relieve the symptoms. Tribulus terrestris (T. terrestris), a flowering plant from family Zygophyllaceae, is used in traditional medicine for treating different disorders and exerts neuroprotective and antioxidant effects in experimental models. The current study attempted to test whether treatment with T. terrestris sta...
Source: Journal of Physiology and Pharmacology - March 23, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

The antiatherogenic effect of new biocompatible cationically modified polysaccharides: chitosan and pullulan - the comparison study.
Authors: Stefan J, Kus K, Wisniewska A, Lorkowska-Zawicka B, Kaminski K, Szczubialka K, Nowakowska M, Korbut R Abstract Pullulan and chitosan are biocompatible polysaccharides obtained from natural sources with many biomedical applications. Cationically modified polymers, such as chitosan and pullulan after covalent attachment of glycidyltrimethylammonium chloride (GTMAC), showed beneficial biological properties. In the present study, it was clearly demonstrated and confirmed that both cationically modified polysaccharides (chitosan-GTMAC and pullulan-GTMAC) have the antiatherosclerotic potential by inhibition of a...
Source: Journal of Physiology and Pharmacology - March 23, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Metabolomic study of altered energy metabolism during global forebrain ischemia and ischemic precoditioning in blood plasma in homocysteine treated rats.
In this study, we investigated metabolic changes in blood plasma in Hcy-treated rats. In combination with Hcy injections to induce hyperhomocysteinemia-like state, we used an animal model of global cerebral ischemia to investigate metabolic changes after 24 h reperfusion in rats. We also focused on the endogenous phenomenon known as ischemic tolerance induced by the preischemic treatment. The experiments were carried out on blood plasma samples as they are easily available and metabolically reflect the overall changes in injured organism. We observed significant changes in plasma metabolite levels of: pyruvate, citrate, ac...
Source: Journal of Physiology and Pharmacology - February 27, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Differential effects of nitric oxide deficiency on primary tumour growth, pulmonary metastasis and prostacyclin/thromboxane A2 balance in orthotopic and intravenous murine models of 4T1 breast cancer.
In conclusion, in a 4T1 murine model of metastatic breast cancer, NO plays a major role in primary tumour development, while NO is not the key mediator of cancer cell extravasation to the lungs. Furthermore, NO-deficiency activates a PGI2-dependent compensatory mechanism only in the intravenous model of 4T1 breast cancer. PMID: 30802213 [PubMed - in process] (Source: Journal of Physiology and Pharmacology)
Source: Journal of Physiology and Pharmacology - February 27, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Elevated plasma levels of tissue factor as a valuable diagnostic biomarker with relevant efficacy for prediction of breast cancer morbidity.
Authors: Zarychta E, Rhone P, Bielawski K, Rosc D, Szot K, Zdunska M, Ruszkowska-Ciastek B Abstract The aim of this study was to evaluate the concentrations of tissue factor (TF) and its inhibitor (TFPI), vascular endothelial growth factor A (VEGF-A), soluble forms of VEGF receptors type 1 and 2 (sVEGFR1 and aVEGFR2) in patients diagnosed with luminal A breast cancer (BrC) and in healthy individuals and to find associations of analyzed factors with demographic, clinical and pathological characteristics in a homogeneous breast cancer group. Study group consisted of 60 women aged 40 - 69 years, diagnosed with luminal...
Source: Journal of Physiology and Pharmacology - February 27, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research