Role of adipokines in the assessment of severity and predicting the clinical course of acute pancreatitis.
Authors: Wos-Wroniewicz E, Caban M, Malecka-Panas E Abstract Acute pancreatitis (AP) is one of the most common diseases requiring hospitalization with increasing incidence. This pathology has variable severity and is associated with significant morbidity and mortality. Early diagnosis, including prognosis of clinical course of the disease is key in the initial clinical management. However, currently available prognostic markers have variable efficacy and the limited utility. Adipokines that are released from the peripancreatic adipose tissue during AP may represent the easy to use and practical AP prognostic marker...
Source: Journal of Physiology and Pharmacology - January 23, 2021 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Inhibition of soluble epoxide hydrolase offers protection against fructose-induced diabetes and related metabolic complications in rats.
Authors: Shah SA, Mehmood MH, Khan M, Bukhari IA, Alorainey BI, Vohra F Abstract Stabilization of epoxyeicosatrienoic acids (EETs) levels via soluble epoxide hydrolase (sEH) deletion or its pharmacological inhibition have been shown to have beneficial effects on inflammation, ischemia, hypertension and diabetes. Owing to the diverse role of EETs, current study was designed to evaluate the therapeutic potential of 1-trifluoromethoxyphenyl-3-(1-propionylpiperidine-4-yl) urea (TPPU), a novel sEHI against fructose-induced diabetes and related complications in rats. Sprague-Dawley rats (200 - 230 g) were divided into fo...
Source: Journal of Physiology and Pharmacology - January 23, 2021 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Extracellular signal-regulated kinases, P38 and c-Jun N-terminal kinases phosphorylation changes in PC12 cells with diabetic disturbances and comorbid excitotoxicity - a preliminary report.
This study investigated ERK, p38 and JNK protein kinases phosphorylation changes in diabetic in vitro conditions with accompanying excitotoxicity reflected by high L-glutamate concentrations. An InstantOne ELISA test in cell lysates was performed to evaluate the intensity of phosphorylation of ERK, p38 and JNK occurring as a result of the incubation of undifferentiated PC12 cells with solutions of glucose (G1,G2), insulin (I1,I2) and L-glutamate (L1,L2). We observed increased phosphorylation of JNK (Thr183/Tyr185) and p38 (Thr180/Tyr182) kinases. For both these kinases, we have shown an increase in phosphorylation in case ...
Source: Journal of Physiology and Pharmacology - January 23, 2021 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Metabolic cardio- and reno-protective effects of empagliflozin in a prediabetic rat model.
Authors: Huttl M, Markova I, Miklankova D, Oliyarnyk O, Trnovska J, Kucera J, Sedlacek R, Haluzik M, Malinska H Abstract The mechanisms behind the cardiovascular and renal benefits of empagliflozin is not fully understood. The positive impact of the medication on cardiovascular mortality can not be solely attributed to its antidiabetic effect, with a metabolic mechanism possibly involved. To investigate the metabolic effects of empagliflozin treatment (10 mg/kg/day for 6 weeks), we used an adult male rat model with serious vascular complications associated with metabolic syndrome and prediabetes. Impaired glucose t...
Source: Journal of Physiology and Pharmacology - January 23, 2021 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Vasoactivity of magnesium sulfate in chicken ductus arteriosus.
In conclusion, MgSO4 induced endothelium-independent relaxation of chicken DA and this relaxation appeared to be mediated through stimulation of BKCa channels and blockade of transmembrane flux of extracellular Ca2+. However, O2-induced DA contraction was only impaired by suprapharmacological concentrations of MgSO4 (> 6.0 mmol L-1). Therefore, our data suggest that the higher incidence of patent DA in preterm infants exposed to MgSO4 is unlikely to be due to a direct pharmacological effect of the drug on the DA. PMID: 33475092 [PubMed - in process] (Source: Journal of Physiology and Pharmacology)
Source: Journal of Physiology and Pharmacology - January 23, 2021 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Multicenter analysis of endoclot as hemostatic powder in different endoscopic settings of the upper gastrointestinal tract.
Authors: Hagel AF, Raithel M, Hempen P, Preclik G, Dauth W, Neurath MF, Gschossman J, Konturek PC, Albrecht H Abstract Gastrointestinal bleeding (GIB) still presents a demanding situation with high morbidity and mortality rates; thus hemostatic powders such as EndoClot (EC) have been developed to improve endoscopic armament. The aim of the present study was to determine which indications triggered the application of EC and to assess resulting hemostasis rates. Forty three patients undergoing endoscopical procedures in three hospitals; two tertiary care and one university hospital, were included. EC was applied in 4...
Source: Journal of Physiology and Pharmacology - January 23, 2021 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Myrtus communis improves cognitive impairment in renovascular hypertensive rats.
This study investigated the possible effects of Myrtus communis on changes in the cognitive functions of experimental renovascular hypertensive rats. Fifty-six Wistar-Albino rats were equally divided into 4 groups; sham-operated control, renovascular hypertension (RVH), ramipril (RVH + Ram) and Myrtus communis extract (RVH + MC) treatment groups. Goldblatt's 2-kidney 1-clip (2K1C) method was used to induce RVH. At the end of 9 weeks of treatment, after blood pressure recording, the animals underwent new object recognition test and Morris water maze (MWM) task. Following these tests, blood brain barrier (BBB) integrity was ...
Source: Journal of Physiology and Pharmacology - January 23, 2021 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Cigarette smoke extraxt influences intracellular calcium concentration in A549 cells.
In this study, we investigated how CSE influences intracellular calcium concentration in human lung adenocarcinoma A549 cells. The CSE concentrations used (0.4, 2, 3%) did not influence cell viability. However, at these CSE concentrations, calcium influx transient receptor potential vanilloid 4 (TRPV4) and transient receptor potential vanilloid 6 (TRPV6) proteins significantly increased, whereas calcium efflux sodium-calcium exchanger (NCX1) and plasma membrane Ca2+ ATPase (PMCA1) proteins significantly decreased from those of the control cells. The 3% CSE treatment produced an intracellular calcium concentration higher th...
Source: Journal of Physiology and Pharmacology - January 23, 2021 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

The impact of 3,4-methylendioxymetamphetamine (MDMA) abstinence on seeking behavior and the expression of the D2-like and mGlu5 receptors in the rat brain using saturation binding analyses.
This study demonstrates that housing conditions have impact on drug-seeking behavior in rats during abstinence from MDMA self-administration. The observed changes in the dopamine D2-like and mGlu5 receptor Bmax and/or Kd values were brain-region specific and related to either pharmacological and/or motivational features of MDMA. PMID: 33316768 [PubMed - as supplied by publisher] (Source: Journal of Physiology and Pharmacology)
Source: Journal of Physiology and Pharmacology - December 16, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Time course evaluation of lacosamide alone and in polypharmacy on behavioral manifestations and oxidative stress in lithium-pilocarpine-induced model.
Authors: Shakeel W, Javaid S, Anjum SMM, Rasool MF, Samad N, Alasmari F, Alasmari AF, Alaqil FA, Alqarni SA, Alotaibi FM, Alqahtani F, Imran I Abstract The lithium-pilocarpine model in rats is commonly used to study the characteristic events of acute status epilepticus (SE), epileptogenesis and temporal lobe epilepsy (TLE). Here we investigated the impact of lacosamide alone and in combination with other drugs (pregabalin, piracetam and scopolamine) on spontaneous recurrent seizures (SRSs) and behavioral parameters during the time frame of 6 weeks after SE. In addition, the level of oxidative stress in the hippocam...
Source: Journal of Physiology and Pharmacology - December 16, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

The expression profile of genes encoding tumor necrosis factor-alpha, interleukin-6 and their receptor in benign adrenal tumors.
Authors: Morawiec E, Cholewa K, Zenderowski M, Batoryna O, Waluga-Kozlowska E, Komosinska-Vassev K, Krol R, Kajor M, Olczyk P, Waluga M Abstract In the process of neoplasia, during which benign adrenal tumors are formed, stimulators of new blood vessel growth as well as growth of tumor cells are cytokines, especially tumor necrosis factor-alpha (TNF-α) and interleukin 6 (IL-6). We analyzed the expression profile of genes coding: TNF-α, tumor necrosis factor receptor 1 (TNF-R1), TNF-R2, IL-6, interleukin 6 receptor (IL-6R) in sections of adrenocortical tumor tissue, rated on the Weiss point scale, in pat...
Source: Journal of Physiology and Pharmacology - December 16, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Prognostic and predictive roles of DNA mismatch repair status in colon cancer patients treated with oxaliplatin-based chemotherapy: a retrospective study.
This study aims to evaluated the prognostic and predictive roles of DNA mismatch repair status in colon cancer patients treated with oxaliplatin-based chemotherapy. From 2005 to 2008, patients who underwent curative surgical resection for high-risk stage II or stage III colon cancer were recruited in this study. These patients had been received oxaliplatin-based chemotherapy. A total 324 patients were included (41.7% at stage II and 58.3% at stage III), and 59 patients (18.2%) exhibited mismatch repair-deficient (dMMR). The prognostic analysis revealed an increase in disease-free survival (DFS) for dMMR patients versus pro...
Source: Journal of Physiology and Pharmacology - December 16, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Resveratrol reduces excessive cholesterol accumulation in Goto-Kakizaki rat, a model with congenital type 2 diabetes.
Authors: Szkudelska K, Okulicz M, Szkudelski T Abstract Resveratrol (3, 5, 3'-trihydroxystilbene) is a naturally-occurring, biologically active compound having numerous beneficial effects in the organism, including anti-diabetic properties. Its anti-diabetic action have been relatively well established using various animal models, however, in Goto-Kakizaki (GK) rats is poorly explored. These animals are non-obese and have a congenital type 2 diabetes. In the present study, effects of resveratrol on cholesterol content, blood levels of some hormones (thyroxine, triiodothyronine, ghrelin and spexin), glucose and para...
Source: Journal of Physiology and Pharmacology - December 16, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Modulation of sympathoadrenergic contractions by perivascular adipose tissue in mesenteric arteries of rats with different level of body adiposity.
Authors: Zemancikova A, Torok J, Balis P, Valovic P, Ulicna O, Chomova M Abstract Obesity is associated with increased sympathetic nervous system activation, possibly contributing to higher cardiovascular risk. The aim of this study was to assess the relationship between body adiposity and sympathoadrenergic contractions in rat isolated mesenteric arteries, and the modulatory effect of mesenteric perivascular adipose tissue (PVAT). Experiments were performed on male 38-week-old Wistar, Zucker lean (ZL) and Zucker diabetic fatty (ZDF) rats. Paired rings of isolated rat superior mesenteric arteries with or without PV...
Source: Journal of Physiology and Pharmacology - December 16, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Detection of immunoreactive aldosterone in murine gasstrointestinal tract.
Authors: Varmavuori L, Launonen H, Korpela R, Vapaatalo H Abstract The adrenocortical steroid hormone, aldosterone, regulates water and electrolyte transport in the distal tubules and collecting ducts of the nephrons in the kidney. Evidence has accumulated that it participates also in epithelial sodium absorption and potassium excretion in the colon acting via mineralocorticoid receptors. However, it is unclear whether aldosterone, like corticosterone, can be synthetized locally in the gut epithelium. Here we describe for the first time the expression of immunoreactive aldosterone in different sections of the murin...
Source: Journal of Physiology and Pharmacology - December 16, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Nuclear factor- κB - importance, induction of inflammation, and effects of pharmacological modulators in Crohn's disease.
Nuclear factor-κB - importance, induction of inflammation, and effects of pharmacological modulators in Crohn's disease. J Physiol Pharmacol. 2020 Aug;71(4): Authors: Szatkowski P, Krzysciak W, Mach T, Owczarek D, Brzozowski B, Szczeklik K Abstract Crohn's disease (CD) is a chronic inflammatory disease of unknown etiology that covers the entire digestive tract and occurs with periods of remission and clinical exacerbation. CD is most common in North America and Europe, but its incidence is rising rapidly in Asian countries. The pathogenesis of CD is unclear, while genetic predisposition, immune i...
Source: Journal of Physiology and Pharmacology - November 21, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

CD49d and CD49e induce cell adhesion-mediated drug resistance through the nuclear factor- κB pathway in Burkitt lymphoma.
In this study, we investigated the molecular mechanism responsible for CAM-DR in BL cells. We also examined the therapeutic targets of CAM-DR in BL cells and found CD49d and CD49e to be the important adhesion molecules involved. However, CD49a, CD49b, CD11a, CD29, CD18, and CD61 were not found to be associated with CAM-DR in BL cells. Furthermore, we clarified that CD49d- and CD49e-mediated CAM-DR could be attributed to an increase in the expression of B cell leukemia-xL (Bcl-xL) and survivin proteins, and a decrease in the expression of Bcl-2 associated X (Bax), Bcl-2 interacting mediator (Bim) and p53 upregulated modulat...
Source: Journal of Physiology and Pharmacology - November 21, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Quercetin attenuates naso-sinusal inflammation and inflammatory response in lungs and brain on an experimental model of acute rhinosinusitis in rats.
This study aimed to investigate the effect of quercetin without intranasal inflammation and oxidative stress in nasal and sinus mucosa, but also in serum, lungs and brain in a rat model of acute nasal and sinus inflammation induced by administration of lipopolysaccharides (LPS) (from Escherichia coli). Wistar rats were divided into five groups of 10 animals each. The control group received an intranasal saline solution once/day, for seven consecutive days. Rats in groups 2 and 3, received low-dose (5 μg) and high-dose (10 μg) of LPS, once/day, for seven consecutive days. Rats in groups 4 and 5, received low-dose (5 &...
Source: Journal of Physiology and Pharmacology - November 21, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Phase angle and vector analysis from multifrequency segmental bioelectrical impedance analysis: new reference data for older adults.
In conclusion, this is the first study to present SMF-BIA-determined PA and BIVA reference data for healthy subjects aged ≥ 65 years. These data can be used for clinical purposes to identify individuals at increased risk for adverse health events or to monitor treatment responses. PMID: 33214337 [PubMed - in process] (Source: Journal of Physiology and Pharmacology)
Source: Journal of Physiology and Pharmacology - November 21, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Hydrogen sulfide in the nucleus tractus solitarii regulates gastric acid secretion in rats.
Authors: Sun H, Yang J, Shi Y, Wang Y, Li C, Zhu M Abstract Hydrogen sulfide (H2S) is a neuromodulator in the central nervous system. The physiological function of H2S in the nucleus tractus solitarii (NTS) has rarely been reported. This research aimed to explore the role of H2S in regulating gastric functions. Wistar rats were randomly assigned to sodium hydrosulfide (NaHS; 4 and 8 nmol) groups, physiological saline (PS) group, capsazepine (10 pmol) + NaHS (4 nmol) group, L703606 (4 nmol) + NaHS (4 nmol) group, and pyrrolidine dithiocarbamate (4 nmol) + NaHS (4 nmol) group. The pH values of gastric acid were measu...
Source: Journal of Physiology and Pharmacology - November 21, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Cucumis sativus and Cucurbita maxima extract attenuate diabetes-induced hepatic and pancreatic injury in a rat model.
Authors: Atta AH, Saad SA, Atta SA, Mouneir SM, Nasr SM, Desouky HM, Shaker HM Abstract Diabetes is usually associated with oxidative stress that causes hepatic and pancreatic tissue injury. This work was carried out to evaluate the effect of Cucumis sativus and Cucurbita maxima methanol extracts on the streptozotocin-induced diabetic hepatic and pancreatic injury in rats. Diabetes was induced in seven equal groups of rats by a single intraperitoneal injection of streptozotocin (40 mg/kg), in addition to the non-diabetic control group. Two diabetic groups were treated with Cucumis sativus methanol extract and two w...
Source: Journal of Physiology and Pharmacology - November 21, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Plasma fibrin clots of pulmonary embolism patients present increased amounts of factor XIII and alpha2-antiplasmin at 3 months' anticoagulation since the acute phase.
Authors: Zabczyk M, Natorska J, Bagoly Z, Sarkady F, Barath B, Katona E, Bryk A, Zettl K, Wisniewski JR, Undas A Abstract Fibrin cross-linking by coagulation factor (F)XIII leads to clot stabilization. Reduced plasma FXIII levels have been reported in acute pulmonary embolism (PE) patients. We investigated the impact of anticoagulant therapy on clot-bound amounts of FXIII and α2-antiplasmin and their associations with fibrin clot properties in patients with PE. Clots generated from plasma of 18 acute symptomatic patients on admission and after a 3-month treatment with rivaroxaban were assessed off anticoagula...
Source: Journal of Physiology and Pharmacology - November 21, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Potassium-dependent sodium/calcium exchanger 3 (Nckx3) depletion leads to abnormal motor function and social behavior in mice.
This study demonstrates that NCKX3 is involved in behavior and neuronal function. PMID: 33214341 [PubMed - in process] (Source: Journal of Physiology and Pharmacology)
Source: Journal of Physiology and Pharmacology - November 21, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Pharmacological agents and natural compounds: available treatments for osteoporosis.
Authors: Martiniakova M, Babikova M, Omelka R Abstract Osteoporosis, a systemic skeletal disease characterized by a decrease in bone mass and deterioration of bone structure leading to an increased risk of fragility fractures, represents one of the major health problems worldwide. Currently, there are numerous pharmacological products used for the treatment of osteoporosis. Anti-resorptive drugs include bisphosphonates, hormone therapy, selective estrogen-receptor modulators, calcitonin, denosumab, calcium and vitamin D supplementation. Anabolic drugs such as teriparatide, strontium ranelate, romosozumab have recen...
Source: Journal of Physiology and Pharmacology - October 1, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Genetic variant c.711A > T in the hepatobiliary phospholipid transporter ABCB4 is associated with significant liver fibrosis.
Genetic variant c.711A>T in the hepatobiliary phospholipid transporter ABCB4 is associated with significant liver fibrosis. J Physiol Pharmacol. 2020 Jun;71(3): Authors: Smyk W, Weber SN, Hall R, Gruenhage F, Lammert F, Krawczyk M Abstract Liver fibrosis is the common consequence of chronic liver diseases (CLD). Recently liver stiffness measurements (LSM) ≥ 9.1 kPa, as determined by transient elastography (TE), were demonstrated to predict significant fibrosis (stages ≥ F2) in a population-based setting. The PNPLA3 (adiponutrin) p.I148M polymorphism enhances the risk of liver injury. The aim o...
Source: Journal of Physiology and Pharmacology - October 1, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Participation of the intestinal microbiota in the mechanism of beneficial effect of treatment with synbiotic Syngut on experimental colitis under stress conditions.
This study was designed to determine the effect of daily treatment with synbiotic (Syngut) containing inulin, Lactobacillus acidophilus, Bifidobacterium lactis W51, Lactobacillus plantarum W21 and Lactococcus lactis applied i.g. at a dose of 50 mg/kg i.g. on the colonic damage and colonic mucosal blood flow in rats with experimentally induced TNBS-colitis that were additionally exposed or not to acute stress (episodes of cold restraint stress every other day before colitis induction). Control rats received daily treatment with vehicle (saline, i.g.) or mesalazine (50 mg/kg-d i.g.), the standard drug recommended in therapy ...
Source: Journal of Physiology and Pharmacology - October 1, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Investigation of seminal plasma chitotriosidase-1 and leukocyte elastase as potential markers for 'silent' inflammation of the reproductive tract of the infertile male - a pilot study.
Authors: Kratz EM, Zurawska-Plaksej E, Solkiewicz K, Kokot I, Faundez R, Piwowar A Abstract Inflammatory mediators - chitotriosidase-1 (CHIT1) and leukocyte elastase (LE) - were analyzed in human seminal plasma in relation to total antioxidative status (TAS) and pro-inflammatory markers IL-1β and IL-6. Samples collected from 34 males who were part of infertile couples were divided into normozoospermic (N; n = 12, without symptoms of inflammation), oligozoospermic (O; n = 11) and teratozoospermic (T; n = 11) groups. significant differences were observed only in CHIT1 concentration between N and O samples. Howev...
Source: Journal of Physiology and Pharmacology - October 1, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Demetylation of the sex-determining region Y gene promoter and incidence of disorder of sex development in cloned dog males.
In this study, we examine the potential of 5-aza-2'-deoxycytidine (5-aza-dC), a DNA methyltransferase inhibitor, to reduce the phenotypic abnormality XY DSD in somatic cell nuclear transfer (SCNT)- derived pups. We used a 9-year-old normal male German Shepherd dog as a cell donor. Donor cells were treated with 10 nM 5-aza-dC for 4 days before being used for SCNT. At the same stage of cell development, significantly lower levels of DNA methylation of the sex-determining region Y (SRY) promoter was observed in the treated donor cells compared to that in the untreated cells (95.2% versus 53.3% on day 4 for the control and tre...
Source: Journal of Physiology and Pharmacology - October 1, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Resistin is not a useful insulin resistance marker for non-obese patients.
Authors: Pastusiak K, Kregielska-Narozna M, Bogdanski P Abstract Resistin, an adipokine produced in fat tissue, may be linked to insulin resistance (IR) and type 2 diabetes. Previous data are controversial and have focused mainly on obese patients. We aimed to evaluate whether resistin plays a role in the development of IR in normal-weight individuals. The study involved 77 normal-weight participants. We defined IR using different indexes and cut-off points. Resistin, fasting insulin, glucose, and lipids concentrations and anthropometric parameters were measured. Serum resistin concentration was not associated with...
Source: Journal of Physiology and Pharmacology - October 1, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

N-acetylcysteine modulates effect of the iron isomaltoside on peritoneal mesothelial cells.
Authors: Misian M, Baum E, Breborowicz A Abstract Intravenous (i.v.) iron supplementation is used in patients on chronic peritoneal dialysis (pd). Iron induced intraperitoneal inflammation observed in our previous studies with iron sucrose may deteriorate the function of the peritoneum as the dialysis membrane. We evaluated effect iron compound, iron-isomaltoside-100 (IIS) on the peritoneal mesothelial cells (MC). We studied the effect of iv treatment with IIS ± N-acetylcysteine (NAC) on the dialysate parameters and function of MC. In 7 uremic pd patients IIS 200 mg was infused i.v. ± NAC 600 mg. Afte...
Source: Journal of Physiology and Pharmacology - October 1, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Effects of dietary gamma-cyclodextrin on voluntary activity and muscle strength in mice.
Authors: Wupper S, Fischer A, Luersen K, Ipharraguerre IR, Chikamoto K, Furune T, Ishida Y, Terao K, Rimbach G Abstract Gamma-cyclodextrin (γCD) is a cyclic oligosaccharide consisting of eight α-(1,4)-linked glucopyranose subunits, which is often used in the food and pharmaceutical industries. However, little is known regarding the metabolic activity of "empty" γCD per se. Therefore, in the present study young C57BL/6 male mice received a control diet (CON) or an experimental diet that was supplemented with 12.88% γCD exchanged against corn starch. After 6 weeks of treatment, the v...
Source: Journal of Physiology and Pharmacology - October 1, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Efficacy of supplemented Er-xian decoction combined with acupoint application for poor ovarian response.
This study aims to observe the efficacy of supplemented Er-xian decoction combined with acupoint application in treating poor ovarian response (POR). This study was a randomized controlled trial. A total of 80 patients, who were treated in the Affiliated Hospital of Jiangxi University of Traditional Chinese Medicine from January 2016 to December 2017, were divided into two groups by tables of random numbers: experimental group (n = 40), and control group (n = 40). In the experimental group, patients orally received supplemented Er-xian decoction with acupoint application. In the control group, a Kuntai capsule was administ...
Source: Journal of Physiology and Pharmacology - August 13, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

The potential implication of neurokinin B in the modulation of prolactin secretion at the pituitary level in cyclic gilts.
Authors: Czelejewska W, Zmijewska A, Dziekonski M, Okrasa S Abstract Based on the previous studies, neurokinin B (NKB) participation in the modulation of prolactin secretion at the pituitary levelvcan be assumed, but information concerning this topic is largely insufficient. Therefore, in the present study, we aimed: 1) tovevaluate changes in the expression of NKB precursor (Tac3) and its receptor (Tacr3) genes as well as the content of NKB and TACR3 proteins in the porcine anterior pituitary throughout the estrous cycle (days 2 - 3, 9 - 10, 12 - 13, 15 - 16, 19 - 20); 2) to determine in vitro the influence of NKB ...
Source: Journal of Physiology and Pharmacology - August 13, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Effect of natalizumab treatment on metalloproteinases and their inhibitors in a mouse model of multiple sclerosis.
Authors: Pyka-Fosciak G, Lis GJ, Litwin JA Abstract Matrix metalloproteinases (MMPs) regulated by their tissue inhibitors (TIMPs) play a significant role in the pathogenesis of multiple sclerosis (MS) and its mouse model, experimental autoimmune encephalomyelitis (EAE), as they degrade extracellular matrix including vascular basal laminae and by damaging blood-brain barrier (BBB) facilitate transmigration of immune cells into the central nervous system. MMPs are also involved in destruction of myelin sheaths, leading to axonal and neuronal loss. The aim of the present study was to assess whether natalizumab, a tran...
Source: Journal of Physiology and Pharmacology - August 13, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

The activation of complement system in different types of renal replacement therapy.
Authors: Stepniewska J, Dolegowska B, Golembiewska E, Marchelek-Mysliwiec M, Domanski M, Ciechanowski K, Zair L Abstract The complement cascade is a part of innate immune system that responds rapidly to defend the host against invading microorganisms and complete the action of immune cells. The activation of the complement system leads to increased inflammatory response, fibrosis of tubulointestinal tissue and progression of chronic kidney disease (CKD). The purpose of this study was to determine whether the type of renal replacement therapy has an effect on activation of the complement system. The study included 7...
Source: Journal of Physiology and Pharmacology - August 13, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Effect of sex and localization dependent differences of Na,K-ATPase properties in brain of rat.
Authors: Kalocayova B, Vrbjar N Abstract Na,K-ATPase is the main system effectively excluding the superfluous sodium out of the cells on the expense of energy derived from hydrolysis of ATP. In brain 3 different isoforms of the catalytic α-subunit are known. The present study was focused to energy utilization and ability to bind sodium by the Na,K-ATPase as well as expression of all 3 isoforms of the catalytic α-subunit concerning its sex specificity in two selected regions of the brain, in cortex and in cerebellum of rats. Western blot analysis showed higher expression of all 3 catalytic α-subuni...
Source: Journal of Physiology and Pharmacology - August 13, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Interleukin 25, thymic stromal lymphopoietin and house dust mites in pathogenesis of atopic dermatitis.
Authors: Jaworek AK, Szafraniec K, Zuber Z, Wojas-Pelc A, Jaworek J Abstract Atopic dermatitis (AD) is the most common chronic inflammatory skin disease in the world. It is characterized by recurrent eczematous skin lesions, fluctuating course and chronic pruritus. Increasing evidence suggest that AD is more common in adults than previously thought. The disease is characterized by an impaired skin barrier, aberrant Th2-type cytokine production and intensive pruritus. Epithelial keratinocytes constitute the first physical, chemical and immunological barrier, classified as a part of the innate defense system. These k...
Source: Journal of Physiology and Pharmacology - August 13, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Central serous chorioretinopathy induced by drugs metabolized by cytochrome P450 3A4.
Authors: Morawski K, Klonowska A, Kubicka-Trzaska A, Woron J, Romanowska-Dixon B Abstract The purpose of this study was investigate whether replacing or discontinuing drugs that are inhibitors or substrates of cytochrome P450 3A4 (CYP3A4) may improve the clinical course of central serous chorioretinopathy (CSC). A retrospective observational study included 43 patients with active CSC. Twenty seven patients (32 eyes, group 1) were using drugs that act as substrates or inhibitors of CYP3A4. In 25 of these 27 patients, treatments including steroids, calcium channel blockers, anticoagulants, statins, beta-adrenolytics,...
Source: Journal of Physiology and Pharmacology - August 13, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Renin-angiotensin system inhibitors in management of hypertension during the COVID-19 pandemic.
Authors: Dworakowska D, Grossman AB Abstract COVID-19, which is caused by the single-stranded RNA severe acute respiratory syndrome-coronavirus-2 (SARS-CoV-2), has introduced significant therapeutic dilemmas in several areas. One of these is concern regarding the use of renin-angiotensin system (RAS) inhibitors. Dysfunction of the RAS has been observed in COVID-19 patients, but whether RAS inhibitors, such as angiotensin-converting enzyme inhibitors (ACEIs) and angiotensin II type-1 receptor blockers (ARBs), are associated with improved or worse clinical outcomes, remains unclear. RAS inhibitors are currently widel...
Source: Journal of Physiology and Pharmacology - July 9, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

COVID-19 - more than respiratory disease: a gastroenterologist's perspective.
Authors: Konturek PC, Harsch IA, Neurath MF, Zopf Y Abstract Severe acute respiratory syndrome-coronavirus-2 (SARS-CoV2) outbreak is the most dramatic event since World War II. Originating as a cluster of unexplained cases of pneumonia, it turned out that this viral disease termed COVID-19 is not only a respiratory infection, but a systemic disease associated with a number of extrapulmonary complications. One of the medical disciplines that is strongly affected by this viral infection is gastroenterology. COVID-19 causes in some patients typical symptoms of enteritis such as diarrhea or abdominal pain. There is als...
Source: Journal of Physiology and Pharmacology - July 9, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Effect of lithium carbonate on the function of the thyroid gland: mechanism of action and clinical implications.
Authors: Czarnywojtek A, Zgorzalewicz-Stachowiak M, Czarnocka B, Sawicka-Gutaj N, Gut P, Krela-Kazmierczak I, Ruchala M Abstract Lithium carbonate, a drug known for more than 100 years, has been successfully used as a psychiatric medication. Currently, it is a commonly used drug to treat patients with unipolar and bipolar depression, and for the prophylaxis of bipolar disorders and acute mania. Lithium salts may cause the development of goiter, hypothyroidism, or rarely hyperthyroidism. The present review examined the current state of knowledge on the effect of lithium carbonate on the thyroid gland. The Pubmed dat...
Source: Journal of Physiology and Pharmacology - July 9, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Gold nanoparticles phytoreduced with Cornus mas extract mitigate some of gliadin effects on Caco-2 cells.
Authors: Domsa EM, Filip GA, Olteanu D, Baldea I, Clichici S, Muresan A, David L, Moldovan B, Para I, Suciu M, Achim M, Negrean V, Andreica V Abstract Celiac disease (CD) is an autoimmune condition that occurs in genetically predisposed people where the ingestion of gluten produces damage in the small intestine. The treatment accepted until now is a strict gluten free diet. This implies the need for novel or adjuvant treatments, in addition to the standard of care. The present study aimed to assess the effect of gold nanoparticles phytosynthesized with Cornus mas extract (AuCM) compared to Cornus mas extract (CM) a...
Source: Journal of Physiology and Pharmacology - July 9, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Comparison of phenolic compounds, antioxidant and cytotoxic activity of extracts prepared from Japanese quince (Chaenomeles japonica L.) leaves.
Authors: Chojnacka K, Sosnowska D, Polka D, Owczarek K, Gorlach-Lira K, Oliveira de Verasa B, Lewandowska U Abstract Phenolic compounds are very important in the prevention and treatment of many civilization diseases, including colorectal cancer (CRC). In the present study we investigated and compared the phytochemical composition, antioxidant and cytotoxic activity of Japanese quince (Chaenomeles japonica L.) leaves crude phenolic extract (CPE) and purified phenolic-rich extracts (PRE). The UPLC-Q-TOF-MS analysis showed that both extracts contain diversified phenolics compounds (33 - 36 compounds in the PRE and CP...
Source: Journal of Physiology and Pharmacology - July 9, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Second-phase validation study of an alternative developmental toxicity test using mouse embryonic stem cell-derived embryoid bodies.
In conclusion, the EBT can accurately classify various embryotoxicants within a short period and with relatively little effort. Therefore, EBT can be used as a good way to test developmental toxicity. PMID: 32633240 [PubMed - in process] (Source: Journal of Physiology and Pharmacology)
Source: Journal of Physiology and Pharmacology - July 9, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Differences in anti-endothelial and anti-retinal antibody titers: implications for the pathohysiology of acute and chronic central serous chorioretinopathy.
This study provides evidence for the possible involvement of an autoimmune process directed against vessel antigens in the pathogenesis of CSC. AECAs may be more important than ARAs in this disease and may be involved in endothelial damage in the choroidal vessels and choriocapillaris, leading to hyperpermeability, which is central to the pathophysiology of CSC. PMID: 32633241 [PubMed - in process] (Source: Journal of Physiology and Pharmacology)
Source: Journal of Physiology and Pharmacology - July 9, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Mydrane intracameral injection site can be an alternative to mydriatic drops instillation in cataract surgery.
Authors: Nazim-Lipski G, Bolsega-Pacud J, Kubicka-Trzaska A, Markiewicz A, Romanowska-Dixon B Abstract The aim of this study is to compare Mydrane (combination of tropicamide 0.02%, phenylephrine 0.31% and lidocaine 1%) and mydriatic drops (tropicamide 1% and phenylephrine 10%) used for cataract surgery in terms of efficacy in pupil dilation and impact on corneal endothelial cell density (CD) and central corneal thickness (CCT). Prospective study including 64 eyes of 64 patients that underwent phacoemulsification with intraocular lens implantation. Patients were randomized into two groups: Mydrane group received: t...
Source: Journal of Physiology and Pharmacology - July 9, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

4-hydroxynonenal and oxidative stress in several organelles and its damaging effects on cell functions.
Authors: Gallo G, Sprovierio P, Martino G Abstract This review aims to describe the action sites of the oxidative stress products for 4-hydroxy-2E-nonenal, on subcellular fractions of eukaryotic cells from several tissues. Described also are; the detoxification mechanisms from derivatives of 4-hydroxy-2E-nonenal. All dangerous compounds for subcellular fractions are metabolites of a respiratory chain that can give stable products of oxidative compounds and are intermediates of other oxidation reaction chains. Finally, the balancing among the illustrated processes to identify the relative oxidative power of several ...
Source: Journal of Physiology and Pharmacology - June 20, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Effects of whole-body electromyostimulation exercise and caloric restriction on cardiometabolic risk profile and muscle strength in obese women with the metabolic syndrome: a pilot study.
Authors: Reljic D, Konturek PC, Herrmann HJ, Neurath MF, Zopf Y Abstract Obesity, particularly in conjunction with further cardiometabolic risk factors, is associated with an increased risk of cardiovascular disease and mortality. Increased physical activity and dietary modifications are cornerstones of therapeutic interventions to treat obesity and related risk factors. Whole-body electromyostimulation (WB-EMS) has emerged as an innovative, time-efficient type of exercise that can provide positive effects on body composition and muscle strength. However, the impact of WB-EMS on cardiometabolic health in obese indi...
Source: Journal of Physiology and Pharmacology - June 20, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Adropin suppresses insulin expression and secretion in INS-1E cells and rat pancreatic islets.
Authors: Billert M, Jasaszwili M, Strowski M, Nowak KW, Skrzypski M Abstract Adropin is a peptide hormone which is produced in brain and peripheral tissues such as liver. It was found that adropin modulates lipid and glucose homeostasis by interacting with hepatocytes and myocytes. Adropin enhances insulin sensitivity and alleviates hyperinsulinemia in animal models with high-fat diet-induced insulin resistance. However, it is unknown whether adropin regulates insulin secretion and proliferation of beta cells. Therefore, we studied the effects of adropin on insulin secretion in INS-1E cells as well as isolated panc...
Source: Journal of Physiology and Pharmacology - June 20, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Clinical significance of activin A and myostatin in patients with pancreatic adenocarcinoma and progressive weight loss.
Authors: Talar-Wojnarowska R, Wozniak M, Borkowska A, Olakowski M, Malecka-Panas E Abstract Pancreatic ductal adenocarcinoma (PDAC) is characterized by progressive weight loss and nutritional deterioration. Several cytokines, such as activin A and myostatin, ligands of the transforming growth factor-β superfamily, have been shown to influence the pathogenesis of muscle wasting and tumor progression. The aim of our study was to assess the clinical significance of these cytokines in patients with different stages of PDAC. The study included 93 patients: 73 with newly diagnosed PDAC and 20 healthy volunteers as t...
Source: Journal of Physiology and Pharmacology - June 20, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research