4-hydroxynonenal and oxidative stress in several organelles and its damaging effects on cell functions.
Authors: Gallo G, Sprovierio P, Martino G Abstract This review aims to describe the action sites of the oxidative stress products for 4-hydroxy-2E-nonenal, on subcellular fractions of eukaryotic cells from several tissues. Described also are; the detoxification mechanisms from derivatives of 4-hydroxy-2E-nonenal. All dangerous compounds for subcellular fractions are metabolites of a respiratory chain that can give stable products of oxidative compounds and are intermediates of other oxidation reaction chains. Finally, the balancing among the illustrated processes to identify the relative oxidative power of several ...
Source: Journal of Physiology and Pharmacology - June 20, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Effects of whole-body electromyostimulation exercise and caloric restriction on cardiometabolic risk profile and muscle strength in obese women with the metabolic syndrome: a pilot study.
Authors: Reljic D, Konturek PC, Herrmann HJ, Neurath MF, Zopf Y Abstract Obesity, particularly in conjunction with further cardiometabolic risk factors, is associated with an increased risk of cardiovascular disease and mortality. Increased physical activity and dietary modifications are cornerstones of therapeutic interventions to treat obesity and related risk factors. Whole-body electromyostimulation (WB-EMS) has emerged as an innovative, time-efficient type of exercise that can provide positive effects on body composition and muscle strength. However, the impact of WB-EMS on cardiometabolic health in obese indi...
Source: Journal of Physiology and Pharmacology - June 20, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Adropin suppresses insulin expression and secretion in INS-1E cells and rat pancreatic islets.
Authors: Billert M, Jasaszwili M, Strowski M, Nowak KW, Skrzypski M Abstract Adropin is a peptide hormone which is produced in brain and peripheral tissues such as liver. It was found that adropin modulates lipid and glucose homeostasis by interacting with hepatocytes and myocytes. Adropin enhances insulin sensitivity and alleviates hyperinsulinemia in animal models with high-fat diet-induced insulin resistance. However, it is unknown whether adropin regulates insulin secretion and proliferation of beta cells. Therefore, we studied the effects of adropin on insulin secretion in INS-1E cells as well as isolated panc...
Source: Journal of Physiology and Pharmacology - June 20, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Clinical significance of activin A and myostatin in patients with pancreatic adenocarcinoma and progressive weight loss.
Authors: Talar-Wojnarowska R, Wozniak M, Borkowska A, Olakowski M, Malecka-Panas E Abstract Pancreatic ductal adenocarcinoma (PDAC) is characterized by progressive weight loss and nutritional deterioration. Several cytokines, such as activin A and myostatin, ligands of the transforming growth factor-β superfamily, have been shown to influence the pathogenesis of muscle wasting and tumor progression. The aim of our study was to assess the clinical significance of these cytokines in patients with different stages of PDAC. The study included 93 patients: 73 with newly diagnosed PDAC and 20 healthy volunteers as t...
Source: Journal of Physiology and Pharmacology - June 20, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Serum betatrophin and irison levels in hepatocellular carcinoma.
Authors: Pazgan-Simon M, Zuwala-Jagiello J, Menzyk T, Bator M, Derra A, Lekstan A, Grzebyk E, Simon K, Kukla M Abstract Hepatocellular carcinoma (HCC) development is a complex process with well-known risk factors, however the role of betatrophin/angiopoietin-like protein 8 and irisin has been poorly investigated thus far. The aim of this study is to measure betatrophin and irisin serum levels in HCC, cirrhotic patients and controls, assess their relationship with cancer etiology and grade, metabolic abnormalities and liver dysfunction severity. Serum betatrophin and irisin concentrations were measured with commerci...
Source: Journal of Physiology and Pharmacology - June 20, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

The role of the adenosine triphosphate-sensitive potassium channels in pinacidil-induced vasodilatation of the human saphenous vein in patients with and without type 2 diabetes mellitus.
Authors: Rajkovic J, Peric M, Stanisic J, Novakovic R, Djokic V, Rakocevic J, Tepavcevic S, Labudovic-Borovic M, Gostimirovic M, Heinle H, Gojkovic-Bukarica L Abstract Type 2 diabetes mellitus (T2DM) increases cardiovascular complications. Diabetic vascular dysfunction is associated with the reduced activity of the different smooth muscle potassium (K+) channels. Thus, the objective of our study was to investigate the role of the adenosine triphosphate (ATP)-sensitive K+ (KATP) channels in the relaxant effect of potassium channel opener, pinacidil on the human saphenous vein (HSV) obtained from the patients with an...
Source: Journal of Physiology and Pharmacology - June 20, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Extracellular vesicles in follicular fluid of sexually mature gilts' ovarian antral follicles - identification and proteomic analysis.
Authors: Grzesiak M, Popiolek K, Knapczyk-Stwora K Abstract Ovarian follicular growth and development involves extensive bidirectional cell-to-cell communication between somatic cells and the oocyte. Recently it has been found that extracellular vesicles (EVs) represent a new mechanism of the communication inside the ovarian follicle. The present research shows for the first time the presence of EVs in follicular fluid of small (SFs), medium (MFs) and large (LFs) antral follicles of sexually mature gilts using nanoparticle tracking analysis and Western blot. The highest (P = 0.0338) concentration of EVs was found i...
Source: Journal of Physiology and Pharmacology - June 20, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Immunomodulation inhibits the development of endometriosis in rats.
This study determined whether immunomodulation by the RESAN vaccine could alter the potentially pathogenic gene expression profiles in the cells of the eutopic endometrium in an animal model of endometriosis. Preventing these changes could inhibit the early development of the illness and support the success of surgical treatment. Wistar rats were divided into three groups: prophylaxis (vaccinated before ectopic endometrium implantation, n = 23), therapeutic (vaccinated at the time of the ectopic excision, n = 23) and control (n = 10). During the first laparotomy, autotransplantation of the endometrium to the peritoneum was...
Source: Journal of Physiology and Pharmacology - June 20, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Inhibitory effect of alpha-lipoic acid on mitochondrial dysfunction and interleukin-8 expression in interleukin-1beta-stimulated ataxia teleangiectasia fibroblasts.
In conclusion, supplementation with α-LA may be beneficial for reducing the oxidative stress-induced mitochondrial dysfunction and IL-8 production associated with A-T. PMID: 32554850 [PubMed - in process] (Source: Journal of Physiology and Pharmacology)
Source: Journal of Physiology and Pharmacology - June 20, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Effects of direct oral anticoagulants on thromboelastographic parameters and fibrin clot properties in patients with venous thromboembolism.
Authors: Kopytek M, Zabczyk M, Natorska J, Malinowski KP, Undas A Abstract Data concerning the impact of direct oral anticoagulants (DOACs) on the thromboelastography (TEG) indices in venousthromboembolism (VTE) patients are limited. The goal of this study was to compare the impact of DOACs on clot properties measured in whole blood using TEG kaolin test and in plasma obtained from real-life VTE patients. We assessed 53 patients, including 20 on rivaroxaban, 20 on apixaban, and 13 on dabigatran. Using the TEG® 5000, coagulation status was evaluated in whole blood samples, while plasma fibrin clot permeability (...
Source: Journal of Physiology and Pharmacology - May 2, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Celiac disease: a multi-faceted medical condition.
Authors: Domsa EM, Berindan-Neagoe I, Para I, Munteanu L, Matei D, Andreica V Abstract Celiac disease (CD) is a systemic condition of autoimmune origin, affecting genetically predisposed individuals who at some point lose tolerance towards dietary gluten. Prevalence in the general population is 0.5 - 1%, with a higher frequency in women. The most important environmental factor for CD is ingestion of specific gluten peptides. It triggers a sequence of molecular events, involving the intestinal permeability and the immune system, which ends in damage of the intestinal mucosa. A number of studies have demonstrated the...
Source: Journal of Physiology and Pharmacology - May 1, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Assessment of leptin-to-adiponectin ratio in prediction of insulin resistance and nutrition status in a geriatric female population.
Authors: Biercewicz M, Slusarz R, Kedziora-Kornatowska K, Filipska K, Bielawski K, Ruszkowska-Ciastek B Abstract Both obesity and malnutrition leading to cachexia and sarcopenia are relevant risk factors in the development of many diseases. They also increase mortality, also prolong hospitalisations and convalescence, and undoubtedly increase the cost of treatment, mostly in the elderly populations. The aim of the study was to assess the relationship between the levels of leptin and adiponectin with regard to insulin resistance and malnutrition status by studying a senior female population and to evaluate predictor...
Source: Journal of Physiology and Pharmacology - May 1, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Daily oral administration of chlorogenic acid prevents the experimental carrageenan-induced oxidative stress.
Authors: Mitrea DR, Malkey R, Florian TL, Filip A, Clichici S, Bidian C, Moldovan R, Hoteiuc OA, Toader AM, Baldea I Abstract Inflammation and oxidative stress are interrelated processes, during which many pathological processes lead to the reactive oxygen species (ROS) and the cytokines release. The aim of this experimental study was to analyse the effects of chlorogenic acid, in oral daily administration, against the oxidative stress and oedema development in experimental carrageenan-induced rat paw inflammation. The oxidative stress parameters were investigated after a paw inflammation was produced in rats that ...
Source: Journal of Physiology and Pharmacology - May 1, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Spent hops (Humulus Lupulus L.) extract as modulator of the inflammatory response in lipopolysaccharide stimulated RAW 264.7 macrophages.
In this study, the inhibition of the formation of inflammatory mediators by the spent hops extract (SHE), a polyphenol-rich extract from Humulus Lupulus L., was examined using lipopolysaccharide (LPS)- activated murine macrophages (RAW 264.7). The SHE suppressed inter alia the interleukin-6 (IL-6) mRNA expression to 32% in LPS-activated macrophages and to 61% at a protein level (at 25 μg/mL). SHE reduced both the cyclooxygenase-2 (COX-2) mRNA expression to 47% and their protein expression to 32%. Not only did SHE inhibit the IL-6 and COX-2 levels but also decreased both inducible nitric oxide synthase (iNOS) protein exp...
Source: Journal of Physiology and Pharmacology - May 1, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

The influence of retinol concentration in liquid crystal formula on epidermal growth factor, interleukin-6 and transglutaminase-1 mRNA expression in the epidermis.
Authors: Zasada M, Erkiert-Polguj A, Markowicz-Piasecka M, Bakiewicz A, Budzisz E Abstract Topical retinol application effectively reduces the effects of photoaging and improves skin condition, e.g. influences the process of keratinization of the epidermis, which improves stratum corneum structure and reduces transepidermal water loss. However, cosmetics use lower concentrations of retinol, which has been associated with emerging hypersensitivity reactions as well as redness and irritation of the skin. The question arises whether the vehicle used in the cosmetic may be important in stimulating the inflammatory reac...
Source: Journal of Physiology and Pharmacology - May 1, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Human skeletal muscle-derived stem/progenitor cells modified with connexin-43 prevent arrhythmia in rat post-infarction hearts and influence gene expression in the myocardium.
Authors: Rugowska A, Wiernicki B, Maczewski M, Mackiewicz U, Chojnacka K, Bednarek-Rajewska K, Kluk A, Majewski P, Kolanowski T, Malcher A, Rozwadowska N, Kurpisz M Abstract Stem cell therapy in combination with genetic modification (e.g., transfection with the coding sequence for the connexion 43 gene, GJA1) may solve the problems associated with the occurrence of additional (secondary) stimulation in the post-infarcted heart (arrhythmia). Human skeletal muscle-derived stem/progenitor cells (SkMDS/PCs) were transfected with the pCiNeo-GJA1 plasmid at an efficiency of approximately 96%. Gene overexpression was asse...
Source: Journal of Physiology and Pharmacology - March 25, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Anticoagulant-related gastrointestinal bleeding: a real-life data analysis on bleeding profiles, frequency and etiology of patients receiving direct oral anticoagulants versus vitamin K antagonists.
In this study we evaluate the bleeding profiles, frequencies and etiologies of patients receiving DOAC versus VKA in a real-life setting. All patients presenting with suspected gastrointestinal bleeding (GIB) in the emergency department of the University Hospital Erlangen in one year were enrolled in this study. They were looked up for the intake of either DOAC (dabigatran, rivaroxaban and apixaban) or VKA. The results showed that 406 patients with suspected GIB were admitted to the emergency unit of the University Hospital Erlangen. In 228 of those patients GIB could be verified (56.2%). Fifty four of those patients (23.7...
Source: Journal of Physiology and Pharmacology - March 25, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Conjugated linoleic acids diminish glycogen synthase and glycogen synthase kinase-3 expression in muscle cells of C57BL/6J mice - in vitro and in vivo study.
Authors: Szynkowska A, Siwiec E, Gutowska I, Baranowska-Bosiacka I, Szczuko M, Kotlega D, Marlicz W, Stachowska E Abstract Conjugated linoleic acids (CLA) have been extensively advertised as dietary supplements to reduce fat and increase muscle mass. However, the role of CLA in glycogen metabolism is still largely unknown. The aim of this study was to assess the effect of CLA on glycogen synthesis in vitro (CCL 136 cell line human) and CLA in vivo (C57BL/6J mice). The materials used were the CCL 136 muscle cell line and muscles of female C57BL/6J mice (n = 52), housed at animal laboratory facility and feed with &qu...
Source: Journal of Physiology and Pharmacology - March 25, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Modulatory effect of concomitant administration of sitagliptin and vitamin E on inflammatory biomarkers in rats fed with high fat diet: role of adiponectin.
In this study, we investigated the effect of sitagliptin and vitamin E on metabolic dysfunction in high-fat diet (HFD) fed rats. Sixty-four male rats were allocated into 8 groups (n = 8) as follow; control, control + vitamin E, control + sitagliptin, control + sitagliptin + vitamin E, HFD, HFD + vitamin E, HFD + sitagliptin and HFD + sitagliptin + vitamin E. Control groups were fed with chow diet for 15 weeks, while HFD groups were fed with HFD for the same duration. Vitamin E and sitagliptin were administered in the last 4 weeks of the study. At the end of the 15th week, body weight, liver weight/body weight ratio, weight...
Source: Journal of Physiology and Pharmacology - March 25, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Ongoing pain in streptozotocin model of diabetes in the rat: correlation with cutaneous cheminociception.
In conclusion, the present results suggest that the response to topical mustard oil may predict ongoing pain-like behavior in the STZ model of diabetes. PMID: 32203940 [PubMed - in process] (Source: Journal of Physiology and Pharmacology)
Source: Journal of Physiology and Pharmacology - March 25, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Sulodexide modulates the dialysate effect on the peritoneal mesothelium.
Authors: Misian M, Baum E, Breborowicz A Abstract Peritoneal membrane damage during chronic peritoneal dialysis is the main cause of that treatment failure. Preservation of the mesothelial cells (MC) is important for the survival of the peritoneum. Evaluation of dialysates effect on the function of MC and potential modification of that effect by sulodexide (heparin 80% and dermatan sulfate 20%). Dialysate effluents, after the overnight exchange with dianeal 1.5% dextrose, were collected from 7 continuous ambulatory peritoneal dialysis (CAPD) patients, and their effect ± sulodexide 0.5 LRU/mL on genes express...
Source: Journal of Physiology and Pharmacology - March 25, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Oxidative stress and inflammatory markers in prediabetes and diabetes.
Authors: Luc K, Schramm-Luc A, Guzik TJ, Mikolajczyk TP Abstract Prediabetes is a state of elevated plasma glucose in which the threshold for diabetes has not yet been reached and can predispose to the development of type 2 diabetes and cardiovascular diseases. Insulin resistance and impaired beta-cell function are often already present in prediabetes. Hyperglycemia can upregulate markers of chronic inflammation and contribute to increased reactive oxygen species (ROS) generation, which ultimately cause vascular dysfunction. Conversely, increased oxidative stress and inflammation can lead to insulin resistance and ...
Source: Journal of Physiology and Pharmacology - February 23, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

The wine polyphenol resveratrol modulates autophagy and induces apoptosis in MOLT-4 and HL-60 human leukemia cells.
Authors: Siedlecka-Kroplewska K, Wozniak M, Kmiec Z Abstract Resveratrol is a naturally occurring polyphenolic compound present in many plant species and wine. It possesses a wide range of beneficial biological properties including anticancer activity. Resveratrol has been demonstrated to induce both autophagy and apoptosis in several human cancer cell lines. The aim of this study was to investigate whether resveratrol modulates autophagy and apoptosis in MOLT-4 human lymphoblastic leukemia and HL-60 human promyelocytic leukemia cells. Cell viability was evaluated by the neutral red uptake assay. Cell cycle distrib...
Source: Journal of Physiology and Pharmacology - February 23, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Nesfatin-1 protects from acute pancreatitis: role of melanocortin receptors.
Authors: Buzcu H, Ozbeyli D, Yuksel M, Cilingir Kaya OT, Kasimay Cakir O Abstract Nesfatin-1, a recently discovered peptide, was shown to have anti-inflammatory effects. Acute pancreatitis (AP) is a life-threatening condition caused by various reasons. Although the etiology of AP is well-known, its pathogenesis is not clear. The aim of this study is to investigate the possible anti-inflammatory role of nesfatin-1 and its probable protective underlying mechanisms in an acute pancreatitis model. Caerulein was applied intraperitoneally to induce acute pancreatitis in Sprague-Dawley female rats. Nesfatin-1 was administ...
Source: Journal of Physiology and Pharmacology - February 23, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Pancreatitis-associated protein-1 suppresses apoptosis in cerulein-stimulated pancreatic acinar cells in response to nuclear factor-kappa B activation.
Authors: Yu JH, Lim JW, Kim H Abstract Pancreatitis is a disease for which there are numerous etiologies but no effective treatments. Although the expression of the pancreatitis-associated protein-1 (PAP-1) serves as a marker for the disease, its biological function is unknown. The present study was carried out to determine if PAP-1 performs a protective role against oxidative stress-induced pancreatic cell death. For this purpose, we used cerulein-stimulated pancreatic acinar AR42J cells as an experimental model of acute pancreatitis. First, we demonstrated that PAP-1 gene expression is increased by cerulein in a ...
Source: Journal of Physiology and Pharmacology - February 23, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Adropin, nesfatin-1 and angiotensin II receptor expression in the abdominal aorta in ovariectomized rats after nesfatin-1 treatment.
In conclusion, our study showed that the ovariectomy surgery induced changes in the abdominal aorta wall characteristic for aging females. Application of nesfatin-1 may prevent the negative consequences in the vessel wall structure in females in conditions of estrogen deficiency and prevent atherosclerotic changes in the cardiovascular system. PMID: 32084647 [PubMed - as supplied by publisher] (Source: Journal of Physiology and Pharmacology)
Source: Journal of Physiology and Pharmacology - February 23, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Effects of stress preconditioning on vulnerability of gastric and small intestinal mucosa to ulcerogenic action of indomethacin in rats.
Authors: Yarushkina NI, Filaretova LP Abstract The preconditioning effect of a mild stressor can reduce the ulcerogenic effect of a severe stressor on the gastric mucosa. The aim of the study was to investigate the effect of preconditioning stress on the gastric and the small intestinal injury caused by a single injection of indomethacin (IM) in conscious rats. Preliminary fasting (24 hours) rats were subjected IM administration (35 mg/kg, subcutaneously) with preconditioning stress (30 min cold-restraint at 10°C and further 1 hour keeping in cages at room temperature) or without stress. Plasma corticosterone l...
Source: Journal of Physiology and Pharmacology - February 23, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

The role of PPAR gamma agonists - rosiglitazone and 15-deoxy- Δ12,14-prostaglandin J2 in experimental cyclosporine A hepatotoxicity.
The role of PPAR gamma agonists - rosiglitazone and 15-deoxy-Δ12,14-prostaglandin J2 in experimental cyclosporine A hepatotoxicity. J Physiol Pharmacol. 2019 Dec;70(6): Authors: Sikora-Wiorkowska A, Smolen A, Czechowska G, Wiorkowski K, Korolczuk A Abstract Cyclosporine A (CsA) is an immunosuppressive drug used in transplantation and treatment of autoimmune diseases. Experimental studies revealed impairments in liver function and morphology among cyclosporine-treated animals. The aim of the study was to evaluate hepatoprotective activity of peroxisome-proliferator-activated receptors γ (PPA...
Source: Journal of Physiology and Pharmacology - February 23, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Apelin decreased placental hormoine secretion by human trophoblast BeWo cells via apelin receptor, protein kinase A and extracellular signal-regulated kinases 1/2 activation.
In conclusion, our results indicate that apelin can be considered as a gestational hormone implied in the endocrine function of the human placenta, with an important role in controlling the production of steroid and protein hormones in placental BeWo cells. PMID: 32084650 [PubMed - as supplied by publisher] (Source: Journal of Physiology and Pharmacology)
Source: Journal of Physiology and Pharmacology - February 23, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Arterial structure and function and its short- and long-term changes after bariatric surgery.
Authors: Gluszewska A, Gryglewska B, Rewiuk K, Zarzycki B, Dzieza-Grudnik A, Kwater A, Major P, Budzynski A, Gasowski J, Grodzicki T Abstract Subclinical arterial damage connected with endothelial dysfunction is a common denominator of cardiovascular complications in a variety of metabolic diseases, including obesity. The aims of the study was to assess functional vascular changes measured by flow-mediated dilatation (FMD) and nitroglycerin-mediated dilation (NMD) of brachial artery, and to measure vascular structural alterations estimated by carotid intima-media complex thickness (IMT) in short- (10 days) and medi...
Source: Journal of Physiology and Pharmacology - February 23, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Hyperoxia blunts renal sympathetic nerve activity response to acute intermittent hypercapnia in rats.
Authors: Madirazza K, Pecotic R, Pavlinac Dodig I, Valic M, Dogas Z Abstract Activation of the sympathetic nervous system plays an important role in the pathophysiology of sleep-related breathing disorders. The aim of the present study was to examine the effects of different levels of hypercapnia in the presence of various background oxygen levels on the magnitude of sympathoexcitation, measured by the renal sympathetic nerve activity (RSNA) in the acute intermittent hypercapnia (AIHc) rat model. The study was conducted on 56 urethane-anesthetized, vagotomized and mechanically ventilated Sprague-Dawley rats (n = 7/...
Source: Journal of Physiology and Pharmacology - February 5, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Regular cold water swimming during winter time affects resting hematological parameters and serum erythropoietin.
Authors: Checinska-Maciejewska Z, Niepolski L, Checinska A, Korek E, Kolodziejczak B, Kopczynski Z, Krauss H, Pruszynska-Oszmalek E, Kolodziejski P, Gibas-Dorna M Abstract Recreational winter swimming in cold sea water evokes body responses to regularly repeated cold water immersion. However, the understanding of adaptive changes is still limited and data regarding very short-term exposure to severe cold stress are scarce. The purpose of the study was to examine the effects of regular active cold water exposure on resting blood elements and erythropoietin in male and female cold water swimmers (CWSs). Thirty four h...
Source: Journal of Physiology and Pharmacology - February 5, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Managing of ventricular reperfusion tachyarrhythmias - focus on a perfused myocardium.
In this study we tested a hypothesis that reperfusion ventricular tachyarrhythmias can be modified by direct control of repolarization duration in the perfused myocardium during ischemic exposure. After induction of coronary occlusion, three groups of rats were given agencies affecting repolarization duration tetraethylammonium (TEA) 4 mg/kg, n = 9; pinacidil (Pin) 0.3 mg/kg, n = 11, and saline as placebo (control) n = 10. Unipolar electrograms were recorded from ischemic and perfused areas using an array of 64-electrodes to obtain activation times (ATs), repolarization times (RTs), activation-repolarization intervals (ARI...
Source: Journal of Physiology and Pharmacology - February 5, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Possible role of garlic oil in ameliorating renal injury after liver ischemia/reperfusion in rats.
This study investigated the protective effects of garlic oil pretreatment on remote renal functions after liver ischemia/reperfusion (I/R), and clarifying gene expressions of heme oxygenase 1 (HO1), autophagy-related 7 (Atg7) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α) in renal tissues. Thirty six adult female Wistar rats were randomly divided into control, garlic oil-supplemented, liver I/R, and garlic oil-pretreated liver I/R groups. Liver ischemia was performed in anesthetized rats for 45 min, followed by reperfusion for 24 hours in metabolic cages. Serum samples were used for ...
Source: Journal of Physiology and Pharmacology - February 5, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Non-responsiveness and tachyphylaxis to anti-vascular endothelial growth factor treatment in naive patients with exudative age-related macular degeneration.
Authors: Zuber-Laskawiec K, Kubicka-Trzaska A, Karska-Basta I, Pociej-Marciak W, Romanowska-Dixon B Abstract Age-related macular degeneration (AMD) is a leading cause of central visual loss in people aged over 50 years in well developed countries. Although the anti-vascular endothelial growth factor (VEGF) therapy has become a standard treatment for exudative AMD, its effectiveness may be limited in some cases. We aimed to assess the prevalence of non-responsiveness and tachyphylaxis to anti-VEGF drugs in patients with exudative AMD. The study included 63 initially treatment-naive AMD patients who were analyzed for...
Source: Journal of Physiology and Pharmacology - February 5, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Evaluation of selected protein biomarkers of renal function in rats with an experimental model of acute cyclophosphamide-induced cystitis treated with N-acetylcysteine.
Authors: Dobrek L, Nalik-Iwaniak K, Kopanska M, Arent Z, Thor PJ Abstract The administration of cyclophosphamide (CP) is associated with the risk of developing cystitis as well as kidney injury. The aim of the study was to verify the uroprotective effect of N-acetylcysteine (NAC), as well as the evaluation of renal function in the experimental model of acute CP-induced cystitis. Rats from group 1 received intraperitoneally only a single dose of 200 mg/kg b.w. of CP. Individuals from groups 2 and 3 additionally received a single dose of 200 mg/kg b.w. of NAC, respectively, orally (p.o.) and intraperitoneally (i.p.)....
Source: Journal of Physiology and Pharmacology - February 5, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Serum progesterone concentrations on the day of oocyte retrieval above 9.23 ng/ml may predict ovarian hyperstimulation syndrome risk in in vitro fertilized patients.
Authors: Issat T, Nowicka MA, Oleksik TP, Zagozda M, Koziol K, Jakimiuk AA, Lewandowski P, Jakimiuk AJ Abstract The aim of our study was to evaluate serum progesterone levels on the day of oocyte retrieval as a promising biomarker inorder to evaluate the risk of ovarian hyperstimulation syndrome in a group with controlled ovarian hyperstimulation protocols using either gonadotropin antagonists or agonists (GnRH), compare with a natural cycle control group. Patients were divided into 3 groups (148 patients in total): control group in the natural cycle, patients treated with GnRH agonist and patients treated with GnR...
Source: Journal of Physiology and Pharmacology - February 5, 2020 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Cereal grass juice in wound healing: hormesis and cell-survival in normal fibroblasts, in contrast to toxic events in cancer cells.
This study provides insights into the underlying mechanisms through which cereal grass juices activate hermetic adaptation response in normal fibroblasts, and induce cytotoxic and genotoxic events in cancer cells. PMID: 31741456 [PubMed - in process] (Source: Journal of Physiology and Pharmacology)
Source: Journal of Physiology and Pharmacology - November 20, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Lycopene induces apoptosis by inhibiting nuclear translocation of β-catenin in gastric cancer cells.
Lycopene induces apoptosis by inhibiting nuclear translocation of β-catenin in gastric cancer cells. J Physiol Pharmacol. 2019 Aug;70(4): Authors: Kim M, Kim SH, Lim JW, Kim H Abstract Reactive oxygen species (ROS) promote the development and progression of cancer by their effects on several signaling pathways. Lycopene, a major carotenoid natural product, is known to display antioxidant activity and to induce apoptosis of cancer cells. The aim of the present study was to investigate the mechanism by which lycopene induces apoptosis of the human gastric cancer AGS cells. In the present study, we s...
Source: Journal of Physiology and Pharmacology - November 20, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Rapid blood pressure increase after renal denervation in anaesthetized rats: interaction of oxidative stress and neuronal nitric oxide synthase.
Authors: Walkowska A, Vaneckova I, Sadowski J, Kompanowska-Jezierska E Abstract We showed previously that in anaesthetized rats acute noninvasive renal denervation (DNX) induced an increase in arterial blood pressure (MABP), unlike the usual hypotensive effect. Here we aimed to establish the background of such unusual response, especially the role of oxidative stress as suggested by an earlier study. The contribution of oxidative stress was explored by studying the effects on DNX-induced MABP increase of pretreatment with 4-hydroxy-3-methoxyacetophenone (apocynin, APO), a powerful antioxidant and antihypertensive a...
Source: Journal of Physiology and Pharmacology - November 20, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Cyclic nucleotide-dependent relaxation in human umbilical vessels.
Authors: Provitera L, Cavallaro C, Griggio A, Raffaeli G, Amodeo I, Gulden S, Lattuada D, Ercoli G, Lonati C, Tomaselli A, Mosca F, Villamor E Abstract Umbilical vessels have a low sensitivity to dilate, and this property is speculated to have physiological implications. We aimed to investigate the different relaxing responses of human umbilical arteries (HUAs) and veins (HUVs) to agonists acting through the cAMP and cGMP pathways. Vascular rings were suspended in organ baths for isometric force measurement. Following precontraction with the thromboxane prostanoid (TP) receptor agonist U44069, concentration-respons...
Source: Journal of Physiology and Pharmacology - November 20, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Defining sex differences in selected lipid metabolites of blood plasma in Wistar rats.
Authors: Leskanicova A, Chovancova O, Babincak M, Blicharova A, Kolesarova M, Macekova D, Kostolny J, Smajda B, Kiskova T Abstract There is an increasing attention to the role that sex/gender plays in health, behavior and outcomes. Even though we know that males and females are not the same, experiments have sometimes been carried out without considering sex in scientific research. It is essential for scientists and clinicians to consider sex differences as one of the underlying physiological determinants of health and disease to provide the building blocks for evidence-based, individualized medicine. Our work aime...
Source: Journal of Physiology and Pharmacology - November 20, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Subacute exposure to amygdalin influences compact bone remodeling of rabbits.
Authors: Kovacova V, Sarocka A, Omelka R, Bauerova M, Grosskopf B, Formicki G, Kolesarova A, Martiniakova M Abstract Amygdalin is most commonly occurring cyanogenic glycoside. It is found in seeds of many plant species. Our study was aimed to reveal whether pure intramuscularly injected amygdalin or apricot seeds peroral exposure cause changes in bone microstructure of rabbits. Twenty clinically healthy 5 months-old male rabbits were segregated into five groups. Animals from groups A1 and A2 were intramuscularly injected with amygdalin at doses of 0.6 and 3 mg/kg b.w. daily for 28 days. The groups S1 and S2 receive...
Source: Journal of Physiology and Pharmacology - November 20, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Breast cancer surgery decreases serum brain-derived neurotrophic factor concentrations in middle aged women: relationship to the serum C-reactive protein concentration.
Authors: Zoladz JA, Nowak LR, Majerczak J, Kulpa J, Pilc A, Duda K Abstract The aim of this study was to establish the effect of breast cancer surgery in middle aged women on the serum (s) and plasma (p) brain-derived neurotrophic factor concentrations [BDNF]s and [BDNF]p, respectively, in relation to the serum C-reactive protein [CRP]s concentration measured before and at 24 hours after surgery. Eighteen patients with recently diagnosed breast cancer (mean ± SE): age 49.1 ± 1.6 years, body mass 69.8 ± 2.2 kg, BMI 25.8 ± 0.8 kg m-2, participated in this study. The [BDNF]s before the surg...
Source: Journal of Physiology and Pharmacology - October 24, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Angiotensin 1-7 formation in breast tissue is attenuated in breast cancer - a study on the metabolism of angiotensinogen in breast cancer cell lines.
In conclusion, the ability of breast cancer cells to produce numerous angiotensin peptide metabolites was demonstrated. The metabolism of angiotensinogen differed between various types of breast cancer cells. The obtained results indicate the greater importance of the classical pathway - ACE/ANG II/AT1R - in breast cancer cells. The production of ANG 1-12 seems to be marginal in breast tissue, but a tendency for the higher formation of this peptide in cancer cells was observed. The production of ANG 1-7 was significantly lower in cancer cells, whereas the expression of MAS receptor was higher than that in the control. This...
Source: Journal of Physiology and Pharmacology - October 24, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Changes in growth hormone secretion and leptin receptor mRNA expression under the influence of leptin and adrenocorticotropin in pituitary cells of early weaned ewe lambs.
Authors: Kosior-Korzecka U, Wojcik M, Longo V, Puzio I, Nowakiewicz A, Patkowski K, Gregula-Kania M Abstract Early weaning of ewe lambs strongly stimulates the hypothalamic-pituitary-adrenal axis and is associated with suppressed growth rate despite the increased food intake. At the same time, plasma leptin concentration increases only slightly or undetectably. To better understand this atypical interdependence among somatic stress, leptin, and lamb growth rate, we analyzed impact of leptin and/or adrenocorticotropic hormone (ACTH) on growth hormone (GH) secretion as well as the effect of ACTH on mRNA expression of...
Source: Journal of Physiology and Pharmacology - October 24, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Effect of ostarine (enobosarm/GTX024), a selective androgen receptor modulator, on adipocyte metabolism in Wistar rats.
In this study, we investigated the effect of ostarine (enobosarm), a nonsteroidal SARM, on the rat adipocyte metabolism using in vitro techniques. Isolated rat adipocytes were incubated in the presence of different concentrations of ostarine. Control incubation with testosterone as the natural ligand for AR was performed. AR inhibitors were used to investigate the genomic activity of ostarine. Changes in the intensity of lipolysis, lipogenesis, and the secretion of leptin and adiponectin were measured. Moreover, the gene expression of leptin and adiponectin was assessed. For the first time, we have shown that ostarine has ...
Source: Journal of Physiology and Pharmacology - October 24, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Effect of low dose L-NAME pretreatment on nitric oxide/reactive oxygen species balance and vasoactivity in L-NAME/salt-induced hypertensive rats.
In conclusion, these findings indicate that chronic low dose of L-NAME treatment has a potential to trigger adapting mechanisms to attenuate some cardiovascular disorders. PMID: 31642816 [PubMed - in process] (Source: Journal of Physiology and Pharmacology)
Source: Journal of Physiology and Pharmacology - October 24, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

The role of chemokine CCL5/RANTES and metalloproteinase-9 as inflammatory modulators in symptomatic internal carotid artery stenosis.
Authors: Badacz R, Podolec J, Przewlocki T, Siedlinski M, Jozefczuk E, Oleksy H, Baran J, Pieniazek P, Zmudka K, Kablak-Ziembicka A Abstract Up to 80% of all ischemic strokes (IS) attributed to internal carotid athero-occlusive artery stenosis (ICAS) are related to a thromboembolic mechanism. One athero-occlusive ischemic event increases the risk for ischemia in another vascular territory, resulting from inflammation within the atherosclerotic plaque induced by cytokines. Thus, ultrasonographic characteristics of vulnerable plaques in ICAS, including plaque echolucency and ulceration might correspond to cytokine ac...
Source: Journal of Physiology and Pharmacology - October 24, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research

Protective effects of vortioxetine in predator scent stress model of post-traumatic stress disorder in rats: role on neuroplasticity and apoptosis.
Authors: Ozbeyli D, Aykac A, Alaca N, Hazar-Yavuz AN, Ozkan N, Sener G Abstract Post-traumatic stress disorder (PTSD) can be observed after a traumatic event. The effect of an antidepressant vortioxetine (Vrx) against PTSD is unknown. The aim of this study was to investigate the possible protective effect of Vrx in the predator scent-induced PTSD rat model. The rats were exposed to dirty cat litter for 10 min and the protocol was repeated 1 week later with clean cat litter as a trauma reminder. The rats received Vrx (10 mg/kg/p.o.) or saline (1 ml/kg/p.o.) during 7 days between two exposure sessions. Novel object r...
Source: Journal of Physiology and Pharmacology - October 24, 2019 Category: Drugs & Pharmacology Tags: J Physiol Pharmacol Source Type: research