Complete genome sequence of a novel influenza A H1N2 virus circulating in swine from Central Bajio region, Mexico
Summary
The aim of this study was to perform the complete genome sequence of a swine influenza A H1N2 virus strain isolated from a pig in Guanajuato, México (A/swine/Mexico/GtoDMZC01/2014) and to report its seroprevalence in 86 counties at the Central Bajio zone. To understand the evolutionary dynamics of the isolate, we undertook a phylogenetic analysis of the eight gene segments. These data revealed that the isolated virus is a reassortant H1N2 subtype, as its genes are derived from human (HA, NP, PA) and swine (M, NA, PB1, PB2 and NS) influenza viruses. Pig serum samples were analysed by the hemagglutination inhibition...
Source: Transboundary and Emerging Diseases - January 31, 2017 Category: Veterinary Research Authors: J. I. S ánchez‐Betancourt, J. B. Cervantes‐Torres, M. Saavedra‐Montañez, R. A. Segura‐Velázquez Tags: ORIGINAL ARTICLE Source Type: research
Polyanhydride nanovaccine against swine influenza virus in pigs.
Abstract
We have recently demonstrated the effectiveness of an influenza A virus (IAV) subunit vaccine based on biodegradable polyanhydride nanoparticles delivery in mice. In the present study, we evaluated the efficacy of ∼200nm polyanhydride nanoparticles encapsulating inactivated swine influenza A virus (SwIAV) as a vaccine to induce protective immunity against a heterologous IAV challenge in pigs. Nursery pigs were vaccinated intranasally twice with inactivated SwIAV H1N2 (KAg) or polyanhydride nanoparticle-encapsulated KAg (KAg nanovaccine), and efficacy was evaluated against a heterologous zoonotic...
Source: Vaccine - January 19, 2017 Category: Allergy & Immunology Authors: Dhakal S, Goodman J, Bondra K, Lakshmanappa YS, Hiremath J, Shyu DL, Ouyang K, Kang KI, Krakowka S, Wannemuehler MJ, Won Lee C, Narasimhan B, Renukaradhya GJ Tags: Vaccine Source Type: research
Swine Influenza Virus (H1N2) Characterization and Transmission in Ferrets, Chile
N. Bravo-Vasquez et al. (Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - January 9, 2017 Category: Infectious Diseases Source Type: research
Two years of surveillance of influenza a virus infection in a swine herd. results of virological, serological and pathological studies
Publication date: Available online 26 December 2016 Source:Comparative Immunology, Microbiology and Infectious Diseases Author(s): Javier Cappuccio, Marina Dibarbora, Inés Lozada, Alejandra Quiroga, Valeria Olivera, Marta Dángelo, Estefanía Pérez, Hernán Barrales, Carlos Perfumo, Ariel Pereda, Daniel R. Pérez Swine farms provide a dynamic environment for the evolution of influenza A viruses (IAVs). The present report shows the results of a surveillance effort of IAV infection in one commercial swine farm in Argentina. Two cross-sectional serological and virological studies (n=480) were carried out in 201...
Source: Comparative Immunology, Microbiology and Infectious Diseases - December 26, 2016 Category: Infectious Diseases Source Type: research
Whole-Genome Characterization of a Novel Human Influenza A(H1N2) Virus Variant, Brazil
P. Resende et al. (Source: Emerging Infectious Diseases Journal)
Source: Emerging Infectious Diseases Journal - December 1, 2016 Category: Infectious Diseases Source Type: research
Update: Influenza Activity - United States and Worldwide, May 22-September 10, 2016.
Abstract
During May 22-September 10, 2016,* the United States experienced typical low levels of seasonal influenza activity overall; beginning in late August, clinical laboratories reported a slight increase in influenza positive test results and CDC received reports of a small number of localized influenza outbreaks caused by influenza A (H3N2) viruses. Influenza A (H1N1)pdm09, influenza A (H3N2), and influenza B viruses were detected during May-September in the United States and worldwide. The majority of the influenza viruses collected from the United States and other countries during that time have bee...
Source: MMWR Morb Mortal Wkl... - September 24, 2016 Category: Epidemiology Authors: Budd A, Blanton L, Kniss K, Smith S, Mustaquim D, Davlin SL, Kramer N, Flannery B, Fry AM, Grohskopf LA, Olsen SJ, Bresee J, Sessions W, Garten R, Xu X, Elal AI, Gubareva L, Barnes J, Wentworth DE, Burns E, Katz J, Jernigan D, Brammer L Tags: MMWR Morb Mortal Wkly Rep Source Type: research
Circulation of multiple genotypes of H1N2 viruses in a swine farm in Italy over a two-month period
Publication date: Available online 7 September 2016 Source:Veterinary Microbiology Author(s): Maria Serena Beato, Luca Tassoni, Adelaide Milani, Annalisa Salviato, Guido Di Martino, Monica Mion, Lebana Bonfanti, Isabella Monne, Simon James Watson, Alice Fusaro In August 2012 repeated respiratory outbreaks caused by swine influenza A virus (swIAV) were registered for a whole year in a breeding farm in northeast Italy that supplied piglets for fattening. The virus, initially characterized in the farm, was a reassortant Eurasian avian-like H1N1 (H1avN1) genotype, containing a haemagglutinin segment derived from th...
Source: Veterinary Microbiology - September 6, 2016 Category: Veterinary Research Source Type: research
A Novel H1N2 Influenza Virus Related to the Classical and Human Influenza Viruses from Pigs in Southern China
Yafen Song, Xiaowei Wu, Nianchen Wang, Guowen Ouyang, Nannan Qu, Jin Cui, Yan Qi, Ming Liao, Peirong Jiao (Source: Frontiers in Microbiology)
Source: Frontiers in Microbiology - July 7, 2016 Category: Microbiology Source Type: research
Factors associated with herd-level PRRSV infection and age-time to seroconversion in farrow-to-finish herds
Publication date: Available online 16 June 2016 Source:Veterinary Microbiology Author(s): C. Fablet, C. Marois-Créhan, B. Grasland, G. Simon, N. Rose Factors associated with porcine reproductive and respiratory syndrome virus (PRRSV) infection were investigated in 109 herds. Serums from four batches of pigs (4, 10, 16 and 22 weeks, 15 pigs/batch) were tested by ELISA for PRRSV antibodies. Infection by Mycoplasma hyopneumoniae (Mhp), Actinobacillus pleuropneumoniae, H1N1 and H1N2 swine influenza A viruses (swIAV) and PCV2 were detected by specific serological or PCR tests. Data related to herd characteristics...
Source: Veterinary Microbiology - June 16, 2016 Category: Veterinary Research Source Type: research
Protective efficacy of an inactivated Eurasian avian-like H1N1 swine influenza vaccine against homologous H1N1 and heterologous H1N1 and H1N2 viruses in mice.
Abstract
Eurasian avian-like H1N1 (EA H1N1) swine influenza viruses are prevalent in pigs in Europe and Asia, but occasionally cause human infection, which raises concern about their pandemic potential. Here, we produced a whole-virus inactivated vaccine with an EA H1N1 strain (A/swine/Guangxi/18/2011, SW/GX/18/11) and evaluated its efficacy against homologous H1N1 and heterologous H1N1 and H1N2 influenza viruses in mice. A strong humoral immune response, which we measured by hemagglutination inhibition (HI) and virus neutralization (VN), was induced in the vaccine-inoculated mice upon challenge. The inact...
Source: Vaccine - June 15, 2016 Category: Allergy & Immunology Authors: Sui J, Yang D, Qiao C, Xu H, Xu B, Wu Y, Yang H, Chen Y, Chen H Tags: Vaccine Source Type: research
Influenza A Viruses Detected in Swine in Southern Germany after the H1N1 Pandemic in 2009
Summary
Infections with influenza A viruses (IAV) are highly prevalent in swine populations, and stable cocirculation of at least three lineages has been well documented in European swine – till 2009. However, since the emergence of the human pandemic pdmH1N1 virus in 2009, which has been (re)introduced into individual swine herds worldwide, the situation has been changing. These variations in the respective IAV pools within pig populations are of major interest, and the zoonotic potential of putative emerging viruses needs to be evaluated. As data on recent IAV in swine from southern Germany were relatively sparse, the ...
Source: Zoonoses and Public Health - May 31, 2016 Category: Infectious Diseases Authors: J. Pippig, M. Ritzmann, M. Büttner, A. Neubauer‐Juric Tags: Original Article Source Type: research
Pathogenic characteristics of a novel triple-reasserted H1N2 swine influenza virus
Publication date: Available online 24 May 2016 Source:Biologicals Author(s): Huili Liu, Jie Tao, Pengchao Zhang, Xiuchen Yin, Zhuo Ha, Chunling Zhang A novel triple reasserted H1N2 virus A/swine/Shanghai/1/2007 (SH07) was isolated from nasal swabs of weaned pig showing clinical symptoms of coughing and sneezing. To explore the virus characteristics, mice, chickens and pigs were selected for pathogenicity study. Pigs inoculated intranasally with 106 TCID50 SH07 showed clinical symptoms with coughing and sneezing, but no death. The virus nuclear acid was detected in many tissues using real-time PCR, which was...
Source: Biologicals - May 23, 2016 Category: Biology Source Type: research
Pathogenic characteristics of a novel triple-reasserted H1N2 swine influenza virus.
Abstract
A novel triple reasserted H1N2 virus A/swine/Shanghai/1/2007 (SH07) was isolated from nasal swabs of weaned pig showing clinical symptoms of coughing and sneezing. To explore the virus characteristics, mice, chickens and pigs were selected for pathogenicity study. Pigs inoculated intranasally with 10(6) TCID50 SH07 showed clinical symptoms with coughing and sneezing, but no death. The virus nuclear acid was detected in many tissues using real-time PCR, which was mainly distributed in respiratory system particularly in the lungs. The virus was low-pathogenic to chickens with 10(6) TCID50 dose inocu...
Source: Biologicals : Journal of the International Association of Biological Standardization - May 22, 2016 Category: Biotechnology Authors: Liu H, Tao J, Zhang P, Yin X, Ha Z, Zhang C Tags: Biologicals Source Type: research
Molecular characterization of a novel reassortant H1N2 influenza virus containing genes from the 2009 pandemic human H1N1 virus in swine from eastern China
Abstract
Pandemic outbreaks of H1N1 swine influenza virus have been reported since 2009. Reassortant H1N2 viruses that contain genes from the pandemic H1N1 virus have been isolated in Italy and the United States. However, there is limited information regarding the molecular characteristics of reassortant H1N2 swine influenza viruses in eastern China. Active influenza surveillance programs in Zhejiang Province identified a novel H1N2 influenza virus isolated from pigs displaying clinical signs of influenza virus infection. Whole-genome sequencing was performed and this strain was compared with other infl...
Source: Virus Genes - May 6, 2016 Category: Genetics & Stem Cells Source Type: research
Age at vaccination and timing of infection do not alter vaccine-associated enhanced respiratory disease in influenza A virus infected pigs.
Abstract
Whole inactivated virus (WIV) vaccines are widely used in the swine industry to reduce clinical disease against homologous influenza A virus (IAV) infection. In pigs experimentally challenged with antigenically distinct heterologous IAV of the same hemagglutinin subtype, WIV vaccinates have been shown to develop vaccine-associated enhanced respiratory disease (VAERD). We evaluated the impact of vaccine valency, age at vaccination, and duration between vaccination and challenge on development of VAERD using vaccine containing δ1-H1N2 and challenge with pandemic H1N1 (pH1N1) virus. Pigs were vaccin...
Source: Clinical and Vaccine Immunology - March 29, 2016 Category: Allergy & Immunology Authors: Souza CK, Rajão DS, Loving CL, Gauger PC, Pérez DR, Vincent AL Tags: Clin Vaccine Immunol Source Type: research
