Usefulness of a new immunochromatographic assay using fluorescent silica nanoparticles for serodiagnosis of Thai patients with amebiasis
Drug Discov Ther. 2024 Feb 15. doi: 10.5582/ddt.2023.01102. Online ahead of print.ABSTRACTA fluorescence immunochromatography (FIC) kit was developed recently using fluorescent silica nanoparticles coated with a recombinant C-terminal fragment of the surface lectin intermediate subunit (C-Igl) of Entamoeba histolytica to establish rapid serodiagnosis of amebiasis. We further evaluated the system using serum samples from 52 Thai patients with amebiasis. Of the patients, 50 (96%) tested positive using FIC. The samples were also tested using enzyme-linked immunosorbent assay (ELISA) with C-Igl as the antigen. Two samples were...
Source: Drug Discoveries and Therapeutics - February 14, 2024 Category: Drugs & Pharmacology Authors: Azumi Kakino Urassaya Pattanawong Napaporn Kuamsab Tatsuya Imai Chaturong Putaporntip Satomi Asai Xunjia Cheng Somchai Jongwutiwes Hiroshi Tachibana Source Type: research
Molecular docking, dynamics and < em > in vitro < /em > analysis of multi-target inhibitors for < em > Clostridioides difficile < /em >
Bioinformation. 2024 Jan 31;20(1):39-48. doi: 10.6026/973206300200039. eCollection 2024.ABSTRACTThe opportunistic pathogen, Clostridioides difficile owes its extreme pathogenicity for its ability to develop antibiotic resistance and recurrent infections. The current antibiotics used for the treatment are showing declining sensitivity and rising antibiotic resistance. Therefore, it is of interest to develop the anti-clostridial drugs to overcome these issues. Hence, we have explored ZINC library to find the suitable lead compounds against five target proteins of C. difficile. Multistep virtual screening is performed to find...
Source: Bioinformation - February 14, 2024 Category: Bioinformatics Authors: Nikita Chordia Golchha Hasanain Abdulhameed Odhar Anand Nighojkar Sadhana Nighojkar Source Type: research
Usefulness of a new immunochromatographic assay using fluorescent silica nanoparticles for serodiagnosis of Thai patients with amebiasis
Drug Discov Ther. 2024 Feb 15. doi: 10.5582/ddt.2023.01102. Online ahead of print.ABSTRACTA fluorescence immunochromatography (FIC) kit was developed recently using fluorescent silica nanoparticles coated with a recombinant C-terminal fragment of the surface lectin intermediate subunit (C-Igl) of Entamoeba histolytica to establish rapid serodiagnosis of amebiasis. We further evaluated the system using serum samples from 52 Thai patients with amebiasis. Of the patients, 50 (96%) tested positive using FIC. The samples were also tested using enzyme-linked immunosorbent assay (ELISA) with C-Igl as the antigen. Two samples were...
Source: Drug Discoveries and Therapeutics - February 14, 2024 Category: Drugs & Pharmacology Authors: Azumi Kakino Urassaya Pattanawong Napaporn Kuamsab Tatsuya Imai Chaturong Putaporntip Satomi Asai Xunjia Cheng Somchai Jongwutiwes Hiroshi Tachibana Source Type: research
Molecular docking, dynamics and < em > in vitro < /em > analysis of multi-target inhibitors for < em > Clostridioides difficile < /em >
Bioinformation. 2024 Jan 31;20(1):39-48. doi: 10.6026/973206300200039. eCollection 2024.ABSTRACTThe opportunistic pathogen, Clostridioides difficile owes its extreme pathogenicity for its ability to develop antibiotic resistance and recurrent infections. The current antibiotics used for the treatment are showing declining sensitivity and rising antibiotic resistance. Therefore, it is of interest to develop the anti-clostridial drugs to overcome these issues. Hence, we have explored ZINC library to find the suitable lead compounds against five target proteins of C. difficile. Multistep virtual screening is performed to find...
Source: Bioinformation - February 14, 2024 Category: Bioinformatics Authors: Nikita Chordia Golchha Hasanain Abdulhameed Odhar Anand Nighojkar Sadhana Nighojkar Source Type: research
Molecular docking, dynamics and < em > in vitro < /em > analysis of multi-target inhibitors for < em > Clostridioides difficile < /em >
Bioinformation. 2024 Jan 31;20(1):39-48. doi: 10.6026/973206300200039. eCollection 2024.ABSTRACTThe opportunistic pathogen, Clostridioides difficile owes its extreme pathogenicity for its ability to develop antibiotic resistance and recurrent infections. The current antibiotics used for the treatment are showing declining sensitivity and rising antibiotic resistance. Therefore, it is of interest to develop the anti-clostridial drugs to overcome these issues. Hence, we have explored ZINC library to find the suitable lead compounds against five target proteins of C. difficile. Multistep virtual screening is performed to find...
Source: Bioinformation - February 14, 2024 Category: Bioinformatics Authors: Nikita Chordia Golchha Hasanain Abdulhameed Odhar Anand Nighojkar Sadhana Nighojkar Source Type: research
Molecular docking, dynamics and < em > in vitro < /em > analysis of multi-target inhibitors for < em > Clostridioides difficile < /em >
Bioinformation. 2024 Jan 31;20(1):39-48. doi: 10.6026/973206300200039. eCollection 2024.ABSTRACTThe opportunistic pathogen, Clostridioides difficile owes its extreme pathogenicity for its ability to develop antibiotic resistance and recurrent infections. The current antibiotics used for the treatment are showing declining sensitivity and rising antibiotic resistance. Therefore, it is of interest to develop the anti-clostridial drugs to overcome these issues. Hence, we have explored ZINC library to find the suitable lead compounds against five target proteins of C. difficile. Multistep virtual screening is performed to find...
Source: Bioinformation - February 14, 2024 Category: Bioinformatics Authors: Nikita Chordia Golchha Hasanain Abdulhameed Odhar Anand Nighojkar Sadhana Nighojkar Source Type: research
Molecular docking, dynamics and < em > in vitro < /em > analysis of multi-target inhibitors for < em > Clostridioides difficile < /em >
Bioinformation. 2024 Jan 31;20(1):39-48. doi: 10.6026/973206300200039. eCollection 2024.ABSTRACTThe opportunistic pathogen, Clostridioides difficile owes its extreme pathogenicity for its ability to develop antibiotic resistance and recurrent infections. The current antibiotics used for the treatment are showing declining sensitivity and rising antibiotic resistance. Therefore, it is of interest to develop the anti-clostridial drugs to overcome these issues. Hence, we have explored ZINC library to find the suitable lead compounds against five target proteins of C. difficile. Multistep virtual screening is performed to find...
Source: Bioinformation - February 14, 2024 Category: Bioinformatics Authors: Nikita Chordia Golchha Hasanain Abdulhameed Odhar Anand Nighojkar Sadhana Nighojkar Source Type: research
Molecular docking, dynamics and < em > in vitro < /em > analysis of multi-target inhibitors for < em > Clostridioides difficile < /em >
Bioinformation. 2024 Jan 31;20(1):39-48. doi: 10.6026/973206300200039. eCollection 2024.ABSTRACTThe opportunistic pathogen, Clostridioides difficile owes its extreme pathogenicity for its ability to develop antibiotic resistance and recurrent infections. The current antibiotics used for the treatment are showing declining sensitivity and rising antibiotic resistance. Therefore, it is of interest to develop the anti-clostridial drugs to overcome these issues. Hence, we have explored ZINC library to find the suitable lead compounds against five target proteins of C. difficile. Multistep virtual screening is performed to find...
Source: Bioinformation - February 14, 2024 Category: Bioinformatics Authors: Nikita Chordia Golchha Hasanain Abdulhameed Odhar Anand Nighojkar Sadhana Nighojkar Source Type: research
Prevalence and antimicrobial resistance of bacterial enteropathogens in Crete, Greece, during 2011-2022
This study investigated the epidemiology and the antibiotic susceptibility of bacterial enteropathogens among diarrheal patients of all ages in Crete, Greece during 2011-2022. Stool specimens were tested by conventional cultural methods for Salmonella, Shigella, Campylobacter, diarrheagenic Escherichia coli (EPEC, STEC), Yersinia enterocolitica, Aeromonas species and Clostridioides difficile. Antimicrobial susceptibility was determined by the disk diffusion method for Enterobacterales, Campylobacter and Aeromonas, and by the gradient diffusion method for C. difficile. Of the 26,060 stool samples from patients of any age, 1...
Source: Acta Microbiologica et Immunologica Hungarica - February 12, 2024 Category: Microbiology Authors: Sofia Maraki Viktoria Eirini Mavromanolaki Dimitra Stafylaki Evangelia Iliaki-Giannakoudaki Anna Kasimati Source Type: research
The pH-responsive SmrR-SmrT system modulates C. difficile antimicrobial resistance, spore formation, and toxin production
Infection and Immunity, Ahead of Print. (Source: Infection and Immunity)
Source: Infection and Immunity - February 12, 2024 Category: Infectious Diseases Authors: Daniela WetzelZavier A. CarterMarcos P. MonteiroAdrianne N. EdwardsChristopher D. ScharerShonna M. McBride1Department of Microbiology and Immunology, Emory University School of Medicine, Emory Antibiotic Resistance Center, Atlanta, Georgia, USA Nancy E. F Source Type: research
Microscopy methods for Clostridioides difficile
Anaerobe. 2024 Feb 8:102822. doi: 10.1016/j.anaerobe.2024.102822. Online ahead of print.ABSTRACTMicroscopic technologies including light and fluorescent, transmission electron microscopy (TEM), scanning electron microscopy (SEM), and cryo-electron microscopy have been widely utilized to visualize Clostridioides difficile at the molecular, cellular, community, and structural biology level. This comprehensive review summarizes the microscopy tools (fluorescent and reporter system) in their use to study different aspects of C. difficile life cycle and virulence (sporulation, germination) or applications (detection of C. diffi...
Source: Anaerobe - February 10, 2024 Category: Microbiology Authors: Chenlin Hu Kevin W Garey Source Type: research
Rethinking < em > Clostridioides difficile < /em > infection (CDI) surveillance definitions based on changing healthcare utilisation and a more realistic incubation period: reviewing data from a tertiary-referral hospital, Ireland, 2012 to 2021
ConclusionCDI surveillance specifications reflecting healthcare exposure and an incubation period more characteristic of C. difficile may improve targeted CDI prevention interventions.PMID:38333935 | PMC:PMC10853979 | DOI:10.2807/1560-7917.ES.2024.29.6.2300335 (Source: Euro Surveill)
Source: Euro Surveill - February 9, 2024 Category: Infectious Diseases Authors: Mairead Skally Kathleen Bennett Hilary Humphreys Fidelma Fitzpatrick Source Type: research
The burden of Clostridioides difficile infections in South-East Asia and the Western Pacific: A narrative review
CONCLUSION: The regional CDI burden in Asia is still incompletely documented, seemingly due to low awareness and limited laboratory testing. Despite this apparent under recognition, the current CDI burden highlights the need for broader surveillance and for application of preventative measures against CDI in Asia.PMID:38336258 | DOI:10.1016/j.anaerobe.2024.102821 (Source: Anaerobe)
Source: Anaerobe - February 9, 2024 Category: Microbiology Authors: Frederick J Angulo Canna Ghia Mark A Fletcher Egemen Ozbilgili Graciela Morales Source Type: research
Culture-independent detection of low-abundant < em > Clostridioides difficile < /em > in environmental DNA via PCR
Appl Environ Microbiol. 2024 Feb 9:e0127823. doi: 10.1128/aem.01278-23. Online ahead of print.ABSTRACTClostridioides difficile represents a major burden to public health. As a well-known nosocomial pathogen whose occurrence is highly associated with antibiotic treatment, most examined C. difficile strains originated from clinical specimen and were isolated under selective conditions employing antibiotics. This suggests a significant bias among analyzed C. difficile strains, which impedes a holistic view on this pathogen. In order to support extensive isolation of C. difficile strains from environmental samples, we designed...
Source: Applied and Environmental Microbiology - February 9, 2024 Category: Microbiology Authors: Miriam A Sch üler Dominik Schneider Anja Poehlein Rolf Daniel Source Type: research
Immunogenicity and safety in rabbits of a Clostridioides difficile vaccine combining novel toxoids and a novel adjuvant
Vaccine. 2024 Feb 8:S0264-410X(24)00101-4. doi: 10.1016/j.vaccine.2024.01.076. Online ahead of print.ABSTRACTClostridioides difficile infection (CDI) is a serious healthcare-associated disease, causing symptoms such as diarrhea and pseudomembranous colitis. The major virulence factors responsible for the disease symptoms are two secreted cytotoxic proteins, TcdA and TcdB. A parenteral vaccine based on formaldehyde-inactivated TcdA and TcdB supplemented with alum adjuvant, has previously been investigated in humans but resulted in an insufficient immune response. In search for an improved response, we investigated a novel t...
Source: Vaccine - February 9, 2024 Category: Allergy & Immunology Authors: Aria Aminzadeh Luuk Hilgers Peter Paul Platenburg Micka ël Riou No émie Perrot Christelle Rossignol Axel Cauty C éline Barc Ren é Jørgensen Source Type: research
