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Occurrence of vancomycin-resistant Staphylococcus aureus in the oral cavity of patients with dental caries.
Abstract Oral streptococci are the major group of microbes isolated from oral microflora. They represent frequent pathogens of infective endocarditis (IE), and it is assumed that in most of the cases oral streptococci are acquired via mucosa layer of oral cavity. Staphylococcus aureus is also frequently isolated from IE as it accounts for 20%-30% of all cases. Vancomycin has been the most reliable therapeutic agent against infections caused by methicillin-resistant S. aureus (MRSA). The main objective of this study was to examine the occurrence of S. aureus species in dental caries specimens. Antimicrobial suscept...
Source: Acta Microbiologica et Immunologica Hungarica - September 11, 2017 Category: Microbiology Authors: Vellappally S, Divakar DD, Al Kheraif AA, Ramakrishnaiah R, Alqahtani A, Dalati MHN, Anil S, Khan AA, Harikrishna Varma PR Tags: Acta Microbiol Immunol Hung Source Type: research

Resurgence of yellow fever in Brazil: Overview and possible control options.
PMID: 28889757 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - September 11, 2017 Category: Microbiology Authors: Khan AW, Nasim Z, Zahir F, Khan MA, Ali A, Ali S, Khan AH Tags: Acta Microbiol Immunol Hung Source Type: research

MALDI-TOF MS versus 16S rrna sequencing: Minor discrepancy between tools in identification of Bacteroides isolates.
te;czy K, Melegh SZ, Urbán E Abstract Members of the genus Bacteroides are important components of the normal microbiota of gastrointestinal tract; however, as opportunistic pathogens are also associated with severe or even life-threatening infections with significant mortality. Various species within Bacteroides fragilis group are phenotypically very similar; thus, their identifications with traditional-automated biochemical methods are frequently inaccurate. The identification of the newly discovered or reclassified bacteria can be doubtful because of the lack of biochemical profile in the database of the...
Source: Acta Microbiologica et Immunologica Hungarica - September 11, 2017 Category: Microbiology Authors: Sárvári KP, Sóki J, Iván M, Miszti C, Latkóczy K, Melegh SZ, Urbán E Tags: Acta Microbiol Immunol Hung Source Type: research

Efficiency of temporary storage of geothermal waters in a lake system: Monitoring the changes of water quality and bacterial community structures.
In this study, the physical and chemical properties and changes in the bacterial community structure of a reservoir lake system in southeast region of Hungary were monitored and compared through 2 years, respectively. The values of biological oxygen demand, concentrations of ammonium ion, total inorganic nitrogen, total phosphorous, and total phenol decreased, whereas oxygen saturation, total organic nitrogen, pH, and conductivity increased during the storage period. Bacterial community structure of water and sediment samples was compared by denaturing gradient gel electrophoresis (DGGE) following the amplification of the ...
Source: Acta Microbiologica et Immunologica Hungarica - September 5, 2017 Category: Microbiology Authors: Szirányi B, Krett G, Kosáros T, Janurik E, Pekár F, Márialigeti K, Borsodi AK Tags: Acta Microbiol Immunol Hung Source Type: research

High prevalence of OXA-type carbapenemases among Acinetobacter baumannii strains in a teaching hospital of Tehran.
In this study, 100 isolates of CRAB from hospitalized patients during 2015-2016 at Imam Khomeini Hospital were investigated to determine the rates of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains using Kirby-Bauer disk diffusion method. The minimum inhibitory concentrations (MICs) of six antibiotics were determined by broth microdilution method. Multiplex polymerase chain reaction (PCR) was performed to detect blaOXA-51 like and blaOXA-58 like, blaOXA-23 like, and blaOXA-24 like that are encoding resistance to carbapenems. All CRAB isolates were MDR and XDR and 2% of the...
Source: Acta Microbiologica et Immunologica Hungarica - September 5, 2017 Category: Microbiology Authors: Zafari M, Feizabadi MM, Jafari S, Abdollahi A, Sabokbar A Tags: Acta Microbiol Immunol Hung Source Type: research

Emergence of armA and rmtB genes among VIM, NDM, and IMP metallo- β-lactamase-producing multidrug-resistant Gram-negative pathogens.
This study was aimed to establish the pattern of antibiotic sensitivity and relevance of antimicrobial resistance in aerobic Gram-negative bacilli. A total of 103 aerobic Gram-negative bacteria namely Escherichia coli, Klebsiella pneumoniae, Enterobacter spp., Citrobacter koserii, Proteus spp., and Pseudomonas aeruginosa were collected from tertiary care centers around Chennai. Kirby-Bauer Disk Diffusion test and study for genes of cephalosporin, carbapenem, and aminoglycoside resistance were done. A descriptive analysis of the data on altogether 103 clinical urine isolates was performed. All strains showed susceptibility ...
Source: Acta Microbiologica et Immunologica Hungarica - September 5, 2017 Category: Microbiology Authors: Gopalakrishnan S, Kamalanathan A, Rajan S, Bhagat VM, Ali MKS Tags: Acta Microbiol Immunol Hung Source Type: research

First report of coexistence of AmpC beta-lactamase genes in Klebsiella pneumoniae strains isolated from burn patients.
In conclusion, K. pneumoniae is becoming a serious problem in burn patients. Accurate and precise methods and guidelines should be designed for detection of antibiotic-resistant mechanisms. Our data showed the high rate of AmpC beta-lactamase among K. pneumoniae isolated from burn patients, which limit the treatment options. Therefore, the results of this study can provide evidence to help for appropriate treatment of burn patients. PMID: 28859497 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2017 Category: Microbiology Authors: Ghanavati R, Darban-Sarokhalil D, Navab-Moghadam F, Kazemian H, Irajian G, Razavi S Tags: Acta Microbiol Immunol Hung Source Type: research

Influence of human milk oligosaccharides on adherence of bifidobacteria and clostridia to cell lines.
Abstract Adhesion of gut bacteria to the intestinal epithelium is the first step in their colonization of the neonatal immature gut. Bacterial colonization of the infant gut is influenced by several factors, of which the most important are the mode of delivery and breast-feeding. Breast-fed infants ingest several grams of human milk oligosaccharides (HMOs) per day, which can become receptor decoys for intestinal bacteria. The most abundant intestinal bacteria in vaginally delivered infants are bifidobacteria, whereas infants born by cesarean section are colonized by clostridia. The influence of HMOs on the adhesio...
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2017 Category: Microbiology Authors: Musilova S, Modrackova N, Doskocil I, Svejstil R, Rada V Tags: Acta Microbiol Immunol Hung Source Type: research

Prevalence and molecular typing of methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin gene.
Abstract Panton-Valentine leukocidin (pvl) toxin is an important virulence factor of Staphylococcus aureus. The main genes are coa and spa for distinguishing and typing of S. aureus isolates. The aim of this study was to investigate antibiotic resistance, presence of mecA and pvl genes, as well as epidemiological typing of these isolates according to polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in clinical sample isolated from Rasht city, Iran. A total of 250 clinical samples have been isolated from different hospitals. First, isolates of S. aureus were identified through mi...
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2017 Category: Microbiology Authors: Hesari MR, Salehzadeh A, Darsanaki RK Tags: Acta Microbiol Immunol Hung Source Type: research

Emergence of NDM-1 among carbapenem-resistant Klebsiella pneumoniae in Iraqi hospitals.
This study reports NDM-1 for the first time among Klebsiella pneumoniae from hospitalized patients in Baghdad, Iraq. Fifty-five clinical isolates of K. pneumoniae resistant to carbapenem were investigated from burned wounds, sputum, and blood samples. The susceptibility to different antibiotics was tested by VITEK-2 system. All strains were multidrug-resistant and they showed nine different antimicrobial-resistant patterns (A-I) and the most effective antibiotic on these strains was levofloxacin (85.45%). The phenotypic detection of carbapenemases by MASTDISCS D70C revealed 29 (52.73%) strains were MBL-producing, out of 55...
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2017 Category: Microbiology Authors: Hussein NH Tags: Acta Microbiol Immunol Hung Source Type: research

Is there a hormonal regulation of phagocytosis at unicellular and multicellular levels? A critical review.
Abstract Phagocytosis is an ancient cell function, which is similar at unicellular and multicellular levels. Unicells synthesize, store, and secrete multicellular (mammalian) hormones, which influence their phagocytosis. Amino acid hormones, such as histamine, serotonin, epinephrine, and melatonin stimulate phagocytosis, whereas peptide hormones, such as adrenocorticotropic hormone (ACTH), insulin, opioids, arginine vasopressin, and atrial natriuretic peptide decreased it, independently on their chemical structure or function in multicellulars. Macrophage phagocytosis of multicellulars is also stimulated by amino ...
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2017 Category: Microbiology Authors: Csaba G Tags: Acta Microbiol Immunol Hung Source Type: research

High frequency of methicillin-resistant Staphylococcus aureus (MRSA) with SCCmec type III and spa type t030 in Karaj's teaching hospitals, Iran.
In conclusion, high frequency of spa t030 with SCCmec type III and MRSA phenotype illustrated circulating of one of the antibiotic-resistant strains in ICU of Karaj's teaching hospitals and emphasizes the need for ongoing molecular surveillance, antibiotic susceptibility monitoring, and infection control. PMID: 28836817 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - August 24, 2017 Category: Microbiology Authors: Bayat B, Zade MH, Mansouri S, Kalantar E, Kabir K, Zahmatkesh E, Sepehr MN, Naseri MH, Darban-Sarokhalil D Tags: Acta Microbiol Immunol Hung Source Type: research

Colistin resistance associated with outer membrane protein change in  Klebsiella pneumoniae and Enterobacter asburiae.
This study demonstrated that OMP differences were between colistin-susceptible and -resistant counterpart strains. The altered Gram-negative cell wall may contribute to acquired colistin resistance in Enterobacteriaceae. PMID: 28629229 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - June 20, 2017 Category: Microbiology Authors: Kádár B, Kocsis B, Tóth Á, Kristóf K, Felső P, Kocsis B, Böddi K, Szabó D Tags: Acta Microbiol Immunol Hung Source Type: research

Association between Toxoplasma gondii types and outcomes of human infection: A meta-analysis.
Abstract The virulence and pathogenicity of various types of Toxoplasma gondii differ considerably in mice. Recent studies have claimed that similar phenomenon was observed in humans, but no relevant studies have been performed to validate this finding. In addition, reports showing association between a given T. gondii type and outcomes of human infection yielded conflicting results. To provide a more precise estimation of the association and a more reliable conclusion on this subject, we performed this meta-analysis. Relevant literatures were identified in multiple databases and selected based on strict screening...
Source: Acta Microbiologica et Immunologica Hungarica - June 20, 2017 Category: Microbiology Authors: Xia J, Cheng XY, Wang XJ, Peng HJ Tags: Acta Microbiol Immunol Hung Source Type: research

Immune responses to killed reassorted influenza virus supplemented with natural adjuvants.
In this study, we investigated the immunomodulatory effects of a supplemented killed influenza virus (V) by Echinacea purpurea (E) and Nigella sativa (N) extracts and effect of changing the route of immunization from intramuscular (IM) to intraperitoneal (IP). At the 2(nd)-, 3(rd)- and 4(th)-week post-IM immunizations (WPIMI), the supplemented V with N (VN) induced the most significant IgM response unlike N alone. At the 2(nd) WPIMI, V or VN induced the highest significant IgG levels. At the 2(nd)-week post-IP immunization (WPIPI), E and VN induced the most significant IgG levels. Both at the 3(rd) and 4(th) WPIMI or WPIPI...
Source: Acta Microbiologica et Immunologica Hungarica - June 19, 2017 Category: Microbiology Authors: Razin MAF, Osman A, Ali MA, Bahgat MM, Maghraby AS Tags: Acta Microbiol Immunol Hung Source Type: research

Complex multicellular functions at a unicellular eukaryote level: Learning, memory, and immunity.
Abstract According to experimental data, eukaryote unicellulars are able to learn, have immunity and memory. Learning is carried out in a very primitive form, and the memory is not neural but an epigenetic one. However, this epigenetic memory, which is well justified by the presence and manifestation of hormonal imprinting, is strong and permanent in the life of cell and also in its progenies. This memory is epigenetically executed by the alteration and fixation of methylation pattern of genes without changes in base sequences. The immunity of unicellulars is based on self/non-self discrimination, which leads to t...
Source: Acta Microbiologica et Immunologica Hungarica - June 19, 2017 Category: Microbiology Authors: Csaba G Tags: Acta Microbiol Immunol Hung Source Type: research

Protection against shigellosis caused by Shigella dysenteriae serotype 4 in guinea pigs using Escherichia albertii DM104 as a live vaccine candidate strain.
In this study, we assessed three different immunization routes, such as intranasal, oral, and intrarectal routes, and revealed differences in immune responses by measuring both the serum IgG and mucosal IgA antibody titers. Protective efficacy of different routes of immunization was also determined by challenging immunized guinea pigs against live S. dysenteriae. It was found that intranasal immunization showed promising results in terms of antibody response and protective efficacy. All these results reconfirm our previous findings and additionally point out that the intranasal immunization of the environmental E. albertii...
Source: Acta Microbiologica et Immunologica Hungarica - June 9, 2017 Category: Microbiology Authors: Chowdhury FM, Rahman MZ, Sarkar MMH, Rabbi F, Khan SI, Ahsan CR, Birkeland NK Tags: Acta Microbiol Immunol Hung Source Type: research

A gain-of-function mutation of STAT1: A novel genetic factor contributing to chronic mucocutaneous candidiasis.
Abstract Heterozygous gain-of-function (GOF) mutations in the signal transducer and activator of transcription 1 (STAT1) have increasingly been identified as a genetic cause of autosomal-dominant (AD) chronic mucocutaneous candidiasis (CMC). In this article, we describe a 33-year-old man who experienced chronic refractory candidiasis, recurrent otitis media, and pneumonia resulting in bronchiectasis, severe oral and esophageal candidiases with strictures associated with hypothyroidism and immune hemolytic anemia. His son also suffered from persistent candidiasis, chronic diarrhea, poor weight gain, and pneumonia t...
Source: Acta Microbiologica et Immunologica Hungarica - June 9, 2017 Category: Microbiology Authors: Eslami N, Tavakol M, Mesdaghi M, Gharegozlou M, Casanova JL, Puel A, Okada S, Arshi S, Bemanian MH, Fallahpour M, Molatefi R, Seif F, Zoghi S, Rezaei N, Nabavi M Tags: Acta Microbiol Immunol Hung Source Type: research

A deletion mutation in nfxB of in vitro-induced moxifloxacin-resistant Pseudomonas aeruginosa confers multidrug resistance.
In this study, MDR mechanism in Pseudomonas aeruginosa induced via moxifloxacin (MOX) pressure was investigated. After serial MOX [concentration of 0.5 × the minimum inhibitory concentration (MIC)] exposure, the fully susceptible P. aeruginosa ATCC 9027 strain has increased its MIC not only toward MOX (1→128 mg/L) but also to other antibiotics. Furthermore, this MOX-exposed strain did not revert to antibiotic-sensitive phenotype when being cultured in antibiotic-free medium for 12 days. No mutation was observed for FQ-target (gyrA and parC) or most investigated efflux regulatory genes (mexT, m...
Source: Acta Microbiologica et Immunologica Hungarica - May 31, 2017 Category: Microbiology Authors: Thai VC, Pham HV, Nguyen DNM, Lambert P, Nguyen TTH Tags: Acta Microbiol Immunol Hung Source Type: research

High prevalence of NDM metallo- β-lactamase among ESBL-producing Escherichia coli İsolates.
This study aimed to determine the frequency of β-lactamase genes and antibiotic resistance rates of 140 extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates obtained from urinary tract infection in Ordu Province, Turkey. Isolates were identified by classic methods and by automated system. ESBL production was confirmed by double disk synergy test and antimicrobial susceptibility was investigated by disk diffusion method. All isolates were screened for β-lactamase coding genes from three groups (A, B, and D) by polymerase chain reaction. The highest rate of susceptible isolates was observed...
Source: Acta Microbiologica et Immunologica Hungarica - April 19, 2017 Category: Microbiology Authors: Çetinkol Y, Sandalli C, Çalgin MK, Yildirim AA, Akyildiz E, Karaman E, Çiçek AÇ Tags: Acta Microbiol Immunol Hung Source Type: research

Advances in diagnosis and treatment of Helicobacter pylori infection.
Abstract Helicobacter pylori is a Gram-negative motile bacterium causative agent of acute and chronic digestive and extra-digestive human infections. According to different reports worldwide, H. pylori symptomatic and asymptomatic infections are a global problem. The statistical investigations show a percentage of 50 for people who are involved in H. pylori acute/chronic digestive and/or extra-digestive infections around the world. This review focuses on digestive and extra-digestive diseases caused by H. pylori, the related virulence factors, diagnostic techniques including non-invasive and invasive diagnostics a...
Source: Acta Microbiologica et Immunologica Hungarica - March 5, 2017 Category: Microbiology Authors: Ranjbar R, Behzadi P, Farshad S Tags: Acta Microbiol Immunol Hung Source Type: research

Effect of primycin on growth-arrested cultures and cell integrity of Staphylococcus aureus.
Abstract Bactericidal effect against non-dividing bacteria is a very advantageous, but rare characteristic among antimicrobial agents, mostly possessed by those affecting the cell membrane. These kinds of agents can kill bacterial cells without lysis. We assessed these characteristics on primycin, a topical anti-staphylococcal agent highly effective against prevalent multiresistant strains, as it also acts on the cell membrane. In time-kill studies, primycin preserved its bactericidal activity against growth-arrested Staphylococcus aureus cultures. The bactericidal action was slower against growth-arrested culture...
Source: Acta Microbiologica et Immunologica Hungarica - March 5, 2017 Category: Microbiology Authors: Feiszt P, Schneider G, Emődy L Tags: Acta Microbiol Immunol Hung Source Type: research

Study on the glutathione metabolism of the filamentous fungus Aspergillus nidulans.
;, Pócsi I Abstract Yeast protein sequence-based homology search for glutathione (GSH) metabolic enzymes and GSH transporters demonstrated that Aspergillus nidulans has a robust GSH uptake and metabolic system with several paralogous genes. In wet laboratory experiments, two key genes of GSH metabolism, gcsA, and glrA, encoding γ-l-glutamyl-l-cysteine synthetase and glutathione reductase, respectively, were deleted. The gene gcsA was essential, and the ΔgcsA mutant required GSH supplementation at considerably higher concentration than the Saccharomyces cerevisiae gsh1 mutant (8-10 mmol l(-1) vs....
Source: Acta Microbiologica et Immunologica Hungarica - March 5, 2017 Category: Microbiology Authors: Bakti F, Király A, Orosz E, Miskei M, Emri T, Leiter É, Pócsi I Tags: Acta Microbiol Immunol Hung Source Type: research

First description of a rifampicin-resistant Neisseria meningitidis serogroup Y strain causing recurrent invasive meningococcal disease in Hungary.
;szka Z, Bognár Z, Erdősi T Abstract A Hungarian soldier previously immunized against Neisseria meningitidis by quadrivalent polysaccharide vaccine was twice infected with meningococci within six weeks. The patient was treated with ceftriaxone during both episodes and he successfully recovered. His close contacts received rifampicin prophylaxis. An investigation was performed to characterize the genetic background of the pathogens to ascertain if the recurrent invasive meningococcal disease was caused by the same strain and to find out the reason for reinfection. Both meningococci belonged to the fine type...
Source: Acta Microbiologica et Immunologica Hungarica - February 20, 2017 Category: Microbiology Authors: Tóth Á, Berta B, Tirczka T, Jekkel C, Ábrahám A, Prohászka Z, Bognár Z, Erdősi T Tags: Acta Microbiol Immunol Hung Source Type: research

Epidemiology and antibiotic sensitivity of Staphylococcus aureus nasal carriage in children in Hungary.
S, Dobay O Abstract The aim of this study was to assess the Staphylococcus aureus nasal carriage rate in healthy children all over Hungary and to specify some risk factors, the antibiotic resistance patterns of the bacteria, and their genetic relatedness. In total, 878 children (aged 3-6 years) were screened at 21 day-care centers in 16 different cities in Hungary, between February 2009 and December 2011. Samples taken from both nostrils were cultured on blood agar, and suspected S. aureus isolates were identified by β-hemolysis, catalase positivity, clump test, and nucA PCR. Methicillin-resistant strains we...
Source: Acta Microbiologica et Immunologica Hungarica - February 20, 2017 Category: Microbiology Authors: Laub K, Tóthpál A, Kardos S, Dobay O Tags: Acta Microbiol Immunol Hung Source Type: research

Biofilm formation of Achromobacter xylosoxidans on contact lens.
Abstract Achromobacter spp. may contaminate lenses, lens cases, and contact lens solutions and cause ocular infections. The aim of this study was to investigate the possibility of isolated strain of Achromobacter xylosoxidans to form biofilm on the surface of soft contact lenses (CL), to quantify the production of the formed biofilm, and compare it with the reference strains (Pseudomonas aeruginosa, Staphylococcus aureus, and Haemophilus influenzae). Bacterial strain isolated from one contact lens case was identified as A. xylosoxidans using Vitek2 Automated System. Biofilm forming capacity of isolated stra...
Source: Acta Microbiologica et Immunologica Hungarica - February 19, 2017 Category: Microbiology Authors: Konstantinović N, Ćirković I, Đukić S, Marić V, Božić DD Tags: Acta Microbiol Immunol Hung Source Type: research

Virulotyping of Shigella spp. isolated from pediatric patients in Tehran, Iran.
Abstract Shigellosis is a considerable infectious disease with high morbidity and mortality among children worldwide. In this survey the prevalence of four important virulence genes including ial, ipaH, set1A, and set1B were investigated among Shigella strains and the related gene profiles identified in the present investigation, stool specimens were collected from children who were referred to two hospitals in Tehran, Iran. The samples were collected during 3 years (2008-2010) from children who were suspected to shigellosis. Shigella spp. were identified throughout microbiological and serological tests and then s...
Source: Acta Microbiologica et Immunologica Hungarica - February 19, 2017 Category: Microbiology Authors: Ranjbar R, Bolandian M, Behzadi P Tags: Acta Microbiol Immunol Hung Source Type: research

Synthesis and characterization of some abundant nanoparticles, their antimicrobial and enzyme inhibition activity.
Abstract Although the antimicrobial activity of the engineered nanoparticles (NPs) is well known, the biochemical mechanisms underlying this activity are not clearly understood. Therefore, four NPs with the highest global production, namely SiO2, TiO2, ZnO, and Ag, were synthesized and characterized. The synthesized SiO2, TiO2, ZnO, and Ag NPs exhibit an average size of 11.12, 13.4, 35, and 50 nm, respectively. The antimicrobial activity of the synthesized NPs against bacteria and fungi were also determined. NPs-mediated inhibition of two very important enzymes, namely urease and DNA polymerase, is also repor...
Source: Acta Microbiologica et Immunologica Hungarica - February 19, 2017 Category: Microbiology Authors: Khan ST, Malik A, Wahab R, Abd-Elkader OH, Ahamed M, Ahmad J, Musarrat J, Siddiqui MA, Al-Khedhairy AA Tags: Acta Microbiol Immunol Hung Source Type: research

Novel adenovirus detected in kowari (Dasyuroides byrnei) with pneumonia.
V, Bányai K, Farkas SL Abstract A male kowari (Dasyuroides byrnei) originating from a zoo facility was delivered for post mortem evaluation in Hungary. Acute lobar pneumonia with histopathologic changes resembling an adenovirus (AdV) infection was detected by light microscopic examination. The presence of an AdV was confirmed by obtaining partial sequence data from the adenoviral DNA-dependent DNA-polymerase. Although the exact taxonomic position of this novel marsupial origin virus could not be determined, pairwise identity analyses and phylogenetic calculations revealed that it is distantly related to oth...
Source: Acta Microbiologica et Immunologica Hungarica - February 14, 2017 Category: Microbiology Authors: Gál J, Mándoki M, Sós E, Kertész P, Koroknai V, Bányai K, Farkas SL Tags: Acta Microbiol Immunol Hung Source Type: research

Molecular detection of aminoglycoside-modifying enzyme genes in Acinetobacter baumannii clinical isolates.
This study showed a high prevalence rate of AME genes in A. baumannii. This prevalence rate has explained that further aminoglycoside resistance genes may have role in the resistance of clinical isolates of A. baumannii. Therefore, control and treatment of serious infections caused by this opportunistic pathogen should be given more consideration. PMID: 27981851 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - December 15, 2016 Category: Microbiology Authors: Heidary M, Salimi Chirani A, Khoshnood S, Eslami G, Atyabi SM, Nazem H, Fazilati M, Hashemi A, Soleimani S Tags: Acta Microbiol Immunol Hung Source Type: research

Antimicrobial resistance in Enterococcus strains isolated from healthy domestic dogs.
Abstract Enterococci are opportunistic bacteria that cause severe infections in animals and humans, capable to acquire, express, and transfer antimicrobial resistance. Susceptibility to 21 antimicrobial agents was tested by the disk diffusion method in 222 Enterococcus spp. strains isolated from the fecal samples of 287 healthy domestic dogs. Vancomycin and ampicillin minimum inhibitory concentrations (MICs) and high-level aminoglycoside resistance (HLAR) tests were also performed. Isolates showed resistance mainly to streptomycin (88.7%), neomycin (80.6%), and tetracycline (69.4%). Forty-two (18.9%) isolates show...
Source: Acta Microbiologica et Immunologica Hungarica - December 14, 2016 Category: Microbiology Authors: Bertelloni F, Salvadori C, Lotti G, Cerri D, Ebani VV Tags: Acta Microbiol Immunol Hung Source Type: research

Detection of acrA, acrB, aac(6')-Ib-cr, and qepA genes among clinical isolates of Escherichia coli and Klebsiella pneumoniae.
CONCLUSION: The distribution of qepA, aac(6')-Ib-cr, acrA, and acrB resistance determinants in E. coli and K. pneumoniae is a great concern. Therefore, infection control and prevention of spread of drug-resistant bacteria need careful management of medication and identification of resistant isolates. PMID: 27936860 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - December 11, 2016 Category: Microbiology Authors: Heidary M, Bahramian A, Hashemi A, Goudarzi M, Omrani VF, Eslami G, Goudarzi H Tags: Acta Microbiol Immunol Hung Source Type: research

Effects of hemin, CO2, and pH on the branching of Candida albicans filamentous forms.
J, Pócsi I Abstract Morphological transitions of wild-type and oxidative stress-tolerant Candida albicans strains were followed in the RPMI-FBS culture medium at pH values and CO2 levels characteristic for the anatomical niches inhabited by this opportunistic human pathogen fungus, including the oral cavity as well as the intestinal and vaginal lumens. Selected cultures were also supplemented with hemin modeling bleedings. Germination as well as elongation and branching of hyphae were monitored in the cultures using time-lapse video microscopy. Unexpectedly, branching time, which is defined as the time take...
Source: Acta Microbiologica et Immunologica Hungarica - December 11, 2016 Category: Microbiology Authors: Jakab Á, Antal K, Emri T, Boczonádi I, Imre A, Gebri E, Majoros L, Pfliegler WP, Szarka M, Balla G, Balla J, Pócsi I Tags: Acta Microbiol Immunol Hung Source Type: research

Full-length VP2 gene analysis of canine parvovirus reveals emergence of newer variants in India.
Abstract The canine parvovirus (CPV) infection is a highly contagious and serious enteric disease of dogs with high fatality rate. The present study was taken up to characterize the full-length viral polypeptide 2 (VP2) gene of CPV of Indian origin along with the commercially available vaccines. The faecal samples from parvovirus suspected dogs were collected from various states of India for screening by PCR assay and 66.29% of samples were found positive. Six CPV-2a, three CPV-2b, and one CPV-2c types were identified by sequence analysis. Several unique and existing mutations have been noticed in CPV types analyz...
Source: Acta Microbiologica et Immunologica Hungarica - December 11, 2016 Category: Microbiology Authors: Nookala M, Mukhopadhyay HK, Sivaprakasam A, Balasubramanian B, Antony PX, Thanislass J, Srinivas MV, Pillai RM Tags: Acta Microbiol Immunol Hung Source Type: research

Composition of endophytic fungal community associated with leaves of maize cultivated in south Brazilian field.
The objective of this study was to conduct a survey about fungi associated with leaves from two different maize plant lineages and to analyze their microbiota diversity. Isolated fungi were identified by morphological analysis and molecular taxonomy was performed using ITS1-5.8S-ITS2 rDNA. About 27 fungi morphotypes were obtained, 15 of them were from the first maize lineage. About 86.7% of the individuals belonged to the Dothideomycetes class (Phoma sorghina, Epicocum nigrum, Cladosporium sp., Bipolaris zeicola, and Alternaria alternata complex) and 13.3% to the Sordariomycetes class (Diaporthe/Phomopsis sp. and Nigrospor...
Source: Acta Microbiologica et Immunologica Hungarica - December 11, 2016 Category: Microbiology Authors: Szilagyi-Zecchin VJ, Adamoski D, Gomes RR, Hungria M, Ikeda AC, Kava-Cordeiro V, Glienke C, Galli-Terasawa LV Tags: Acta Microbiol Immunol Hung Source Type: research

The in vitro effect of Garden Thyme (Thymus vulgaris L.) extract on Brachyspira hyodysenteriae.
Abstract Filtrates of a water extract of commercially available garden thyme (Thymus vulgaris L.) were used for studying its possible bactericidal effect on Brachyspira hyodysenteriae, the causative agent of swine dysentery, by agar-diffusion technique. Five of the six studied Brachyspira strains have proven to be sensitive and one moderately sensitive in the in vitro tests. It was concluded that water extract of garden thyme possesses inhibitory effects against B. hyodysenteriae. In vivo experiments are needed to check the validity of this conclusion. PMID: 27929352 [PubMed - as supplied by publisher] (Sourc...
Source: Acta Microbiologica et Immunologica Hungarica - December 7, 2016 Category: Microbiology Authors: Kutasi J, Jakab L, Jurkovich V, Rafai P Tags: Acta Microbiol Immunol Hung Source Type: research

Enhanced survival but not amplification of Francisella spp. in the presence of free-living amoebae.
In this study, axenic FLA cultures of Acanthamoeba castellanii, Acanthamoeba polyphaga, and Vermamoeba vermiformis were each inoculated with virulent strains of F. tularensis (Types A and B), the attenuated live vaccine strain, and Francisella novicida. Experimental parameters included low and high multiplicity of infection and incubation temperatures of 25 and 30 °C for 0-10 days. Francisella spp. survival was enhanced by the presence of FLA; however, bacterial growth and protozoa infectivity were not observed. In contrast, co-infections of A. polyphaga and Legionella pneumophila, used as an amoeba pathogen control, r...
Source: Acta Microbiologica et Immunologica Hungarica - December 7, 2016 Category: Microbiology Authors: Buse HY, Schaefer FW, Rice EW Tags: Acta Microbiol Immunol Hung Source Type: research

Differences in MRSA prevalence and resistance patterns in a tertiary center before and after joining an international program for surveillance of antimicrobial resistance.
Abstract Methicillin-resistant Staphylococcus aureus (MRSA) emerged as one of the most important causes of hospital-acquired bloodstream infections (BSIs), especially the multidrug resistant clones. The aim of the present study was to compare prevalence and resistance patterns of MRSA bacteremia in the major tertiary-care academic and referral center in Serbia before and after implementing an active antimicrobial resistance (AMR) surveillance. Laboratory-based before-after study was conducted during a two-year period (January 2012 to December 2013) in Clinical Centre of Serbia. Isolation and identification of bact...
Source: Acta Microbiologica et Immunologica Hungarica - December 7, 2016 Category: Microbiology Authors: Djuric O, Jovanovic S, Stosovic B, Tosic T, Jovanovic M, Nartey N, Todorovic J, Markovic-Denic L Tags: Acta Microbiol Immunol Hung Source Type: research

Role of health care professionals in preventative activities and vaccination programs during outbreaks.
;s Á Abstract PURPOSE: The general purpose of TELL ME study was to give an insight into the experiences of European family physicians with management of H1N1 pandemic flu. METHODS: Qualitative research methods (focus group discussions, one-to-one interviews, and online data collection) were used to explore family doctors' opinion and suggestions. Overall 158 family physicians took part in the study from six European countries. RESULTS: Family doctors' most important experience was that the official campaign was not able to compensate negative effects of the mass media. Due to the poor evidence-bas...
Source: Acta Microbiologica et Immunologica Hungarica - November 15, 2016 Category: Microbiology Authors: Balogh S, Papp R, Busa C, Csikós Á Tags: Acta Microbiol Immunol Hung Source Type: research

Identification of two substrates of FTS_1067 protein - An essential virulence factor of Francisella tularensis.
Abstract Francisella tularensis is a highly virulent intracellular pathogen with the capacity to infect a variety of hosts including humans. One of the most important proteins involved in F. tularensis virulence and pathogenesis is the protein DsbA. This protein is annotated as a lipoprotein with disulfide oxidoreductase/isomerase activity. Therefore, its interactions with different substrates, including probable virulence factors, to assist in their proper folding are anticipated. We aimed to use the immunopurification approach to find DsbA (gene locus FTS_1067) interacting partners in F. tularensis subsp. holarc...
Source: Acta Microbiologica et Immunologica Hungarica - November 14, 2016 Category: Microbiology Authors: Spidlova P, Senitkova I, Link M, Stulik J Tags: Acta Microbiol Immunol Hung Source Type: research

Role of bolA and rpoS genes in biofilm formation and adherence pattern by Escherichia coli K-12 MG1655 on polypropylene, stainless steel, and silicone surfaces.
This study has been undertaken to address the adherence pattern and formation of biofilms by E. coli on stainless steel, polypropylene, and silicone surfaces after 24 h of growth at 37 °C. Scanning electron microscopy was used for direct examination of the cell attachment and biofilm formation on various surfaces and it was found that, in the presence of bolA, E. coli cells were able to attach to the stainless steel and silicone very well. By contrast, polypropylene surface was not found to be attractive for E. coli cells. This indicates that bolA responded and can play a major role in the presence and absenc...
Source: Acta Microbiologica et Immunologica Hungarica - November 14, 2016 Category: Microbiology Authors: Adnan M, Sousa AM, Machado I, Pereira MO, Khan S, Morton G, Hadi S Tags: Acta Microbiol Immunol Hung Source Type: research

Aflatoxigenic Aspergillus flavus and Aspergillus parasiticus strains in Hungarian maize fields.
S, Kriszt B Abstract Due to the climate change, aflatoxigenic Aspergillus species and strains have appeared in several European countries, contaminating different agricultural commodities with aflatoxin. Our aim was to screen the presence of aflatoxigenic fungi in maize fields throughout the seven geographic regions of Hungary. Fungi belonging to Aspergillus section Flavi were isolated in the ratio of 26.9% and 42.3% from soil and maize samples in 2013, and these ratios decreased to 16.1% and 34.7% in 2014. Based on morphological characteristics and the sequence analysis of the partial calmodulin gene, all isolate...
Source: Acta Microbiologica et Immunologica Hungarica - November 13, 2016 Category: Microbiology Authors: Sebők F, Dobolyi C, Zágoni D, Risa A, Krifaton C, Hartman M, Cserháti M, Szoboszlay S, Kriszt B Tags: Acta Microbiol Immunol Hung Source Type: research

Quantification of biofilm formation on silicone intranasal splints: An in vitro study.
Abstract OBJECTIVES: Biofilms are associated with persistent infections and resistant to conventional therapeutic strategies. The aim of this study was to investigate the quantity of biofilm produced on silicone intranasal splints. METHODS: Quantity of biofilm formation on silicone splints (SS) was tested on 15 strains of Staphylococcus aureus and Moraxella catarrhalis, respectively. Antimicrobial susceptibility testing was performed in accordance with European Committee on Antimicrobial Susceptibility Testing recommendations. RESULTS: All tested strains formed different amounts of biofilm on SS: 66.7% S...
Source: Acta Microbiologica et Immunologica Hungarica - August 31, 2016 Category: Microbiology Authors: Pavlović B, Božić DD, Milovanović J, Jotić A, Djukić V, Djukić S, Konstantinović N, Ćirković I Tags: Acta Microbiol Immunol Hung Source Type: research

DR3 regulation of apoptosis of naive T-lymphocytes in children with acute infectious mononucleosis.
Abstract Acute infectious mononucleosis (AIM) is a widespread viral disease that mostly affects children. Development of AIM is accompanied by a change in the ratio of immune cells. This is provided by means of different biological processes including the regulation of apoptosis of naive T-cells. One of the potential regulators of apoptosis of T-lymphocytes is a death receptor 3 (DR3). We have studied the role of DR3 in the regulation of apoptosis of naive CD4(+) (nTh) and CD8(+) (nCTL) T-cells in healthy children and children with AIM. In healthy children as well as in children with AIM, the activation of DR3 is ...
Source: Acta Microbiologica et Immunologica Hungarica - August 31, 2016 Category: Microbiology Authors: Filatova EN, Anisenkova EV, Presnyakova NB, Utkin OV Tags: Acta Microbiol Immunol Hung Source Type: research

Banana fruits affected by Fusarium post-harvest disease as source of human fusariosis.
PMID: 27682849 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - August 31, 2016 Category: Microbiology Authors: Triest D Tags: Acta Microbiol Immunol Hung Source Type: research

Book ReviewReviews.
;cs M Abstract Review of the book "RNA/DNA and Cancer" written by Joseph G. Sinkovics (Springer International Publishing, Switzerland, 2016, ISBN 978-3-319-22278-3, DOI 10.1007/978-3-319-22279-0). PMID: 27682850 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - August 31, 2016 Category: Microbiology Authors: Nagy O, Takács M Tags: Acta Microbiol Immunol Hung Source Type: research

Laboratory diagnosis of Acanthamoeba keratitis in Hungary.
This study reports seven (7/16; 43.75%) positive cases. Detection of Acanthamoeba in corneal scrapings is based on cultivation and polymerase chain reaction (PCR) combined with the molecular taxonomic identification method. By PCR, seven samples were positive; cultivation was successful for five samples, probably because of the low quantity of samples. Genotype identification was carried out with a real-time fluorescence resonance energy transfer PCR assay based on sequence analysis of the 18S rRNA gene, and sensitivity and specificity were evaluated in comparison with traditional parasitological techniques. All seven dete...
Source: Acta Microbiologica et Immunologica Hungarica - August 30, 2016 Category: Microbiology Authors: Orosz E, Farkas Á, Kucsera I Tags: Acta Microbiol Immunol Hung Source Type: research

Ebola virus disease: Awareness among dental students in Hungary.
CONCLUSION: These results are concerning and the medical universities and dental schools need to act fast to prepare the Hungary's dental (and medical) students and junior doctors for an inevitable influx of infected patients. PMID: 27539229 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - August 18, 2016 Category: Microbiology Authors: Károlyházy K, Fazekas B, Fazekas J, Hermann P, Márton K Tags: Acta Microbiol Immunol Hung Source Type: research

Lectins and Tetrahymena - A review.
Abstract The unicellular ciliate Tetrahymena is a complete organism, one of the most highly developed protozoans, which has specialized organelles performing each of the functions characteristic to the cells of higher ranked animals. It is also able to produce, store, and secrete hormones of higher ranked animals and also react to them. It produces lectins that can bind them and has functions, which are influenced by exogenous lectins. The review lists the observations on the relationship between lectins and Tetrahymena and try to construe them on the basis of the data, which are at our disposal. Considering the d...
Source: Acta Microbiologica et Immunologica Hungarica - August 18, 2016 Category: Microbiology Authors: Csaba G Tags: Acta Microbiol Immunol Hung Source Type: research

Immunomodulatory effects of anti-microbial peptides.
Abstract Anti-microbial peptides (AMPs) were originally thought to exert protecting actions against bacterial infection by disintegrating bacterial membranes. Upon identification of internal bacterial targets, the view changed and moved toward inhibition of prokaryote-specific biochemical processes. However, the level of none of these activities can explain the robust efficacy of some of these peptides in animal models of systemic and cutaneous infections. A rapidly growing panel of reports suggests that AMPs, now called host-defense peptides (HDPs), act through activating the immune system of the host. This inclu...
Source: Acta Microbiologica et Immunologica Hungarica - August 18, 2016 Category: Microbiology Authors: Otvos L Tags: Acta Microbiol Immunol Hung Source Type: research