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Influence of subinhibitory antibiotic concentration on Streptococcus pyogenes adherence and biofilm production.
In this study, the focus was on the effects of sub-MICs of the antibiotics on adherence, hydrophobicity, and biofilm formation by two groups of Streptococcus pyogenes strains, which were responsible for different clinical cases. The aim of this study was to explore the effects of sub-MICs of penicillin, ceftriaxone, erythromycin, and clindamycin on adherence, surface hydrophobicity, and biofilm biomass in two selected collections of group A streptococcus (GAS): strains isolated from carriers (CA) and strains isolated from patients with tonsillopharyngitis (TPh). Isolates were tested for hydrophobicity to xylene, adherence,...
Source: Acta Microbiologica et Immunologica Hungarica - April 24, 2018 Category: Microbiology Authors: Šmitran A, Vuković D, Opavski N, Gajić I, Marinković J, Božić L, Živanović I, Kekić D, Popović S, Ranin L Tags: Acta Microbiol Immunol Hung Source Type: research

Analysis of antigen conservation and inactivation of gamma-irradiated avian influenza virus subtype H9N2.
Abstract Avian influenza (AI) A subtype H9N2 virus belongs to Orthomyxoviridae family and causes low-pathogenic disease AI. The use of gamma-irradiated viral antigens has been developed in the production of effective vaccines. In this research, LPAIV H9N2 strain, A/Chicken/IRN/Ghazvin/2001, was multiplied on SPF eggs and irradiated by a Nordian gamma cell instrument. Irradiated and non-irradiated AI virus (AIV) samples were titrated by EID50 method and hemagglutinin (HA) antigen was analyzed by HA test as the WHO pattern method. Infectivity of irradiated virus was determined by egg inoculation method during four b...
Source: Acta Microbiologica et Immunologica Hungarica - April 24, 2018 Category: Microbiology Authors: Salehi B, Motamedi-Sedeh F, Madadgar O, Khalili I, Ghalyan Chi Langroudi A, Unger H, Wijewardana V Tags: Acta Microbiol Immunol Hung Source Type: research

Effect of amino acid substitution in New Delhi metallo- β-lactamase on carbapenem susceptibility.
Effect of amino acid substitution in New Delhi metallo-β-lactamase on carbapenem susceptibility. Acta Microbiol Immunol Hung. 2018 Apr 13;:1-9 Authors: Düzgün AÖ Abstract The aim of this work was investigation of clinically important amino acid substitutions of NDM-1 variants. A blaNDM-1 gene was cloned into expression vector pET100/D-TOPO. The sequence of NDM-1 variants with substituted amino acids was determined by ClustalW program. A pET100/D-TOPO + blaNDM-1 was used to generate the alanine mutations at different positions, such as NDM-2 (P28A), NDM-3 (D95A), NDM-4...
Source: Acta Microbiologica et Immunologica Hungarica - April 13, 2018 Category: Microbiology Authors: Düzgün AÖ Tags: Acta Microbiol Immunol Hung Source Type: research

Risk factors for Clostridium difficile infections in Baranya County, Southern Hungary.
aacute;cs B, Péterfi Z Abstract In the past decade, Clostridium difficile infections (CDIs) have become a major public health challenge. Their epidemiology has radically changed with a significant rise in the number of cases and an increase in severe episodes. Recurrence and failure of conventional treatments are very common. Furthermore, a spread of CDI has emerged in general population without the usual risk factors (unexposed to antibiotic treatment, young people, etc.). The conventional treatments (metronidazole and vancomycin) are still effective and are the first-line antibiotics with new recommendati...
Source: Acta Microbiologica et Immunologica Hungarica - April 13, 2018 Category: Microbiology Authors: Vigvári S, Sipos D, Kappéter Á, Feiszt Z, Kovács B, Péterfi Z Tags: Acta Microbiol Immunol Hung Source Type: research

The first characterized carbapenem-resistant Bacteroides fragilis strain from Croatia and the case study for it.
;n E, Sóki J Abstract An imipenem-resistant Bacteroides fragilis strain was isolated from the blood of a 72-year-old male patient with a urinary bladder tumor in Osijek, Croatia. This strain was also resistant to ampicillin, piperacillin/tazobactam, cefoxitin, clindamycin, tetracycline, and harbored cfiA, ermF, and tetQ genes where the high-level expression of the cfiA carbapenem-resistant gene was driven by an IS1187 element. Interestingly, despite the carbapenem-resistant feature of the B. fragilis from blood, the patient relatively easily recovered from the bacteremia. It was the first characterized imip...
Source: Acta Microbiologica et Immunologica Hungarica - April 13, 2018 Category: Microbiology Authors: Bogdan M, Perić L, Ördög K, Vuković D, Urbán E, Sóki J Tags: Acta Microbiol Immunol Hung Source Type: research

Gynecological and obstetrical aspects of Enterobius vermicularis infection.
nyi K, Nikolova R, Belics Z Abstract Enterobiasis is an easily recognizable and treatable disease with higher incidence among children. Based on these facts, its clinical importance is usually underestimated. This infection also occurs during adulthood and without treatment, it can cause severe complications in some rare cases. Unnoticed subclinical infection in women can lead to infertility and even to life-threatening conditions. It is also emphasized in this paper that the treatment of identified enterobiasis during pregnancy needs caution. After reviewing the current scientific literature, two gynecological ca...
Source: Acta Microbiologica et Immunologica Hungarica - March 19, 2018 Category: Microbiology Authors: Dezsényi B, Sárközi L, Kaiser L, Tárkányi K, Nikolova R, Belics Z Tags: Acta Microbiol Immunol Hung Source Type: research

Antineoplastic potential of mycotoxins.
Bánfalvi G Abstract Fungal toxins are secondary metabolites, in which many of them were mycotoxins, affecting eukaryotic cells with a broad range of structural and functional variety contributing to the multitude of their classification. This refers to the harmful genotoxic (mutagenic, teratogenic, and carcinogenic) effects of mycotoxins on the one hand, and their cytocidic and antineoplastic properties on the other hand. This "double edged sword" effect could be utilized against the spread of tumors in older patients when the survival is much more important than the mutagenic side effects. To dec...
Source: Acta Microbiologica et Immunologica Hungarica - March 19, 2018 Category: Microbiology Authors: Pócsi I, Király G, Bánfalvi G Tags: Acta Microbiol Immunol Hung Source Type: research

Specific immune responses induced by multi-epitope DNA derived from Mycobacterium tuberculosis DosR antigens.
In this study, two optimized constructs consisting of multi-epitopes DNA derived from three latency antigens Rv2029c, Rv2031c, and Rv2627c fused with or without light chain 3 (LC3) are synthetized. The immunogenicity effectiveness of two DNA constructs was evaluated in the mouse model. LC3-fused multi-epitope DNA construct induced strong specific Th1 immune responses with high increase in IFN-γ+ CD4+ and IL-2+ CD4+ T cell populations (both with p 
Source: Acta Microbiologica et Immunologica Hungarica - March 19, 2018 Category: Microbiology Authors: Moradi J, Izad M, Tabrizi M, Mosavari N, Esmaeili B, Feizabadi MM Tags: Acta Microbiol Immunol Hung Source Type: research

Human herpesvirus 6A and 6B and NK cells.
In this report, we shortly summarize the interactions between HHV-6 and NK cells. PMID: 29512392 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - March 7, 2018 Category: Microbiology Authors: Rizzo R, Di Luca D Tags: Acta Microbiol Immunol Hung Source Type: research

Value of the eazyplex ® CSF direct assay in rapid diagnosis of invasive meningococcal disease - Case report.
Value of the eazyplex® CSF direct assay in rapid diagnosis of invasive meningococcal disease - Case report. Acta Microbiol Immunol Hung. 2018 Mar 07;:1-7 Authors: Karrasch M, Eisenach S, Vogel U, Zinke J, Witte OW, Günther A, Romeike B, Rödel J Abstract There is a need for easy-to-use molecular assays for diagnosis of invasive meningococcal disease. Here, we report the rapid identification of Neisseria meningitidis in a cerebrospinal fluid sample from a patient with purulent meningitis using a commercially available loop-mediated isothermal amplification assay, resulting in a prompt de-e...
Source: Acta Microbiologica et Immunologica Hungarica - March 7, 2018 Category: Microbiology Authors: Karrasch M, Eisenach S, Vogel U, Zinke J, Witte OW, Günther A, Romeike B, Rödel J Tags: Acta Microbiol Immunol Hung Source Type: research

Epidemiological situation of measles in Romania, Italy, and Hungary: On what threats should we focus nowadays?
N, Sziveri S, Bosnyákovits T Abstract Although the prevalence of wild-type measles virus infection has decreased by>90% in Europe, the disease is still not eliminated and has even reemerged with recurrent outbreaks in different countries, including Romania and Italy. Minor outbreaks of Romanian origin were reported from Hungary as well. In Romania, an outbreak has been ongoing since February 2016. As of October 2017, 9,670 measles cases and 35 deaths were registered in the country. The three most affected counties are located next to the Hungarian border. In Italy, until the end of August 2017, 4,477 cas...
Source: Acta Microbiologica et Immunologica Hungarica - February 28, 2018 Category: Microbiology Authors: Orosz L, Gáspár G, Rózsa Á, Rákos N, Sziveri S, Bosnyákovits T Tags: Acta Microbiol Immunol Hung Source Type: research

The "Three Amigos" lurking behind type 1 diabetes: Hygiene, gut microbiota and viruses.
The "Three Amigos" lurking behind type 1 diabetes: Hygiene, gut microbiota and viruses. Acta Microbiol Immunol Hung. 2018 Feb 28;:1-18 Authors: Jakobsen OAJ, Szereday L Abstract Incidence of type 1 diabetes (T1D) is on the rise and yet, despite decades of research, the exact ethology of the disease still remains a mystery. The autoimmune reaction, which ultimately leads to the destruction of pancreatic beta cells, causing insulin deficiency and T1D, is a result of genetic susceptibility and environmental factors. Precisely, what are these environmental factors? Current popular opinion implie...
Source: Acta Microbiologica et Immunologica Hungarica - February 28, 2018 Category: Microbiology Authors: Jakobsen OAJ, Szereday L Tags: Acta Microbiol Immunol Hung Source Type: research

Effect of endocrine disruptor phytoestrogens on the immune system: Present and future.
Abstract Endocrine disruptors (EDs) are bound by steroid receptors, have steroid-like effects, and by this, negatively influence hormone-regulated processes. Phytoestrogens, which are consumed in enormously high amount by man, are also EDs; however, in contrast to industrial or communal EDs, in some cases have beneficial effects. As immune cells have steroid (first of all, estrogen) nuclear and plasma membrane receptors, which bind phytostrogens (genistein, daidzein, etc.), the development, lifespan, and function of them are deeply influenced by phytoestrogens. They can provoke perinatal faulty hormonal imprinting...
Source: Acta Microbiologica et Immunologica Hungarica - February 28, 2018 Category: Microbiology Authors: Csaba G Tags: Acta Microbiol Immunol Hung Source Type: research

Neutrophil CD64 - A potential biomarker in patients with complicated intra-abdominal infections? - A literature review.
Abstract Complicated intra-abdominal infections (cIaIs) respresent a serious cause of morbidity and mortality. Early diagnosis and well-timed treatment can improve patients' outcome, whereas the delay in management often result in rapid progression to circulatory collapse, multiple organ failure, and death. Neutrophil CD64 antigen expression has been studied for several years as infectious and sepsis biomarker and has several characteristics that make it good for clinical employment. It has been suggested to be predictive of positive bacterial cultures and a useful test for management of sepsis and other significa...
Source: Acta Microbiologica et Immunologica Hungarica - February 23, 2018 Category: Microbiology Authors: Dimitrov E, Enchev E, Halacheva K, Minkov G, Yovtchev Y Tags: Acta Microbiol Immunol Hung Source Type: research

Plasmid-mediated quinolone resistance determinants in Enterobacteriaceae from urine clinical samples.
In this study, we demonstrated 17.7% prevalence of PMQR-positive Enterobacteriaceae and first reported qnrD-resistance determinant in Hungary. Altogether, 25 PMQR-positive strains were susceptible or low-level resistant to ciprofloxacin with minimum inhibitory concentration (MIC) between 0.06 and 1 mg/L, suggesting that prevalence of PMQR determinants is underestimated and screening among clinical isolates exhibiting reduced susceptibility is necessary. Fluoroquinolone resistance breakpoints of Enterobacteriaceae were revised in 2017 by European Committee of Antimicrobial Susceptibility Testing indicating ciprofloxacin sus...
Source: Acta Microbiologica et Immunologica Hungarica - February 23, 2018 Category: Microbiology Authors: Szabó O, Gulyás D, Szabó N, Kristóf K, Kocsis B, Szabó D Tags: Acta Microbiol Immunol Hung Source Type: research

Mediation of inflammatory ascites formation induced by macromolecules in mice.
Abstract The first 60-min phase of inflammatory ascites formation was studied by intraperitoneally (i.p.) administered macromolecular inducers: yeast cell wall zymosan binds to specific macrophage receptors, polyethyleneimine (PEI) and concanavalin A (ConA), produces non-covalent cross-links on the surface of various cells, while λ-carrageenan may function as a contact activator. Depletion of peritoneal macrophages was performed by overnight pretreatment with diphtheria toxin in transgenic mice, resulting in a significant (p 
Source: Acta Microbiologica et Immunologica Hungarica - February 23, 2018 Category: Microbiology Authors: Baintner K Tags: Acta Microbiol Immunol Hung Source Type: research

Genetic support of carbapenemases in double carbapenemase producer Klebsiella pneumoniae isolated in the Arabian Peninsula.
end A Abstract Enterobacteriaceae co-producing NDM- and OXA-48-type carbapenemases were encountered in higher frequency in the United Arab Emirates (UAE) than in the neighboring countries in our earlier study. The aim of this investigation was to characterize the seven double carbapenemase producer Klebsiella pneumoniae found in the region to assess factors contributing to their emergence. Three K. pneumoniae ST14 isolated in the UAE harboring blaNDM-1 on IncHI1b and blaOXA-232 on IncColE plasmids were clonally related. Furthermore, two K. pneumoniae from the UAE, ABC106 and ABC137 belonged to ST307 and ST1318, re...
Source: Acta Microbiologica et Immunologica Hungarica - February 23, 2018 Category: Microbiology Authors: Al-Baloushi AE, Pál T, Ghazawi A, Sonnevend A Tags: Acta Microbiol Immunol Hung Source Type: research

Streptococcus pneumoniae quorum sensing and biofilm formation are affected by Thymus daenensis, Satureja hortensis, and Origanum vulgare essential oils.
This study introduced T. daenensis and S. hortensis as new anti-biofilm and QS inhibitor agents with a natural origin. PMID: 29471691 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - February 23, 2018 Category: Microbiology Authors: Sharifi A, Ahmadi A, Mohammadzadeh A Tags: Acta Microbiol Immunol Hung Source Type: research

Bacterial diversity in soils of different Hungarian karst areas.
In this study, diversity examinations on two distinct Hungarian karst areas, Aggtelek and Tapolca, were performed using parallel cultivation and molecular cloning methods. The phylogenetic affiliation of bacterial strains and molecular clones was determined based on their 16S rRNA gene sequences. Bacterial isolates were identified as members of the phyla Actinobacteria, Firmicutes, Proteobacteria, and Bacteroidetes. Besides the taxa identified by cultivation, members of the phyla Chloroflexi, Cyanobacteria, Acidobacteria, Verrucomicrobia, and Gemmatimonadetes were detected by the cloning. The difference in the composition ...
Source: Acta Microbiologica et Immunologica Hungarica - February 23, 2018 Category: Microbiology Authors: Knáb M, Szili-Kovács T, Márialigeti K, Móga J, Borsodi AK Tags: Acta Microbiol Immunol Hung Source Type: research

Study of MazEF, sam, and phd-doc putative toxin-antitoxin systems in Staphylococcus epidermidis.
This study showed the significant prevalence of these systems in pathogenic bacteria and they were equally found in both oxacillin-resistant and oxacillin-susceptible bacteria. The high prevalence of three systems can make them suitable as potential targets for antibiotic therapy. PMID: 29471693 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - February 23, 2018 Category: Microbiology Authors: Behrooz SK, Lida L, Ali S, Mehdi M, Rasoul M, Elnaz O, Farid BT, Gholamreza I Tags: Acta Microbiol Immunol Hung Source Type: research

Assessing the microbial communities inhabiting drinking water networks and nitrifying enrichments with special respect on nitrifying microorganisms.
This study provides a comprehensive microbiological survey of three drinking water networks applying different water treatment processes. Variability of microbial communities was assessed by cultivation-based [nitrifying, denitrifying most probable number (MPN) heterotrophic plate count] and sequence-aided terminal restriction fragment length polymorphism (T-RFLP) analysis. The effect of microbial community composition on nitrifying MPN values was revealed. The non-treated well water samples showed remarkable differences to their corresponding distribution systems regarding low plate count, nitrifying MPN, and the composit...
Source: Acta Microbiologica et Immunologica Hungarica - February 23, 2018 Category: Microbiology Authors: Nagymáté Z, Nemes-Barnás K, Krett G, Márialigeti K Tags: Acta Microbiol Immunol Hung Source Type: research

A rarely isolated Gram-negative bacterium in microbiology laboratories: Leclercia adecarboxylata †.
A rarely isolated Gram-negative bacterium in microbiology laboratories: Leclercia adecarboxylata †. Acta Microbiol Immunol Hung. 2018 Feb 23;:1-4 Authors: Çiçek M, Tuncer Ö, Biçakçigil A, Gürsoy NC, Otlu B, Sancak B PMID: 29471695 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - February 23, 2018 Category: Microbiology Authors: Çiçek M, Tuncer Ö, Biçakçigil A, Gürsoy NC, Otlu B, Sancak B Tags: Acta Microbiol Immunol Hung Source Type: research

The recombinant fusion protein CFP10-ESAT6-dIFN has protective effect against tuberculosis in guinea pigs.
SE, Deineko EV Abstract Development of effective vaccine candidates against tuberculosis is currently the most important challenge in the prevention of this disease since the BCG vaccine fails to guarantee a lifelong protection, while any other approved vaccine with better efficiency is still absent. The protective effect of the recombinant fusion protein ESAT6-CFP10-dIFN produced in a prokaryotic expression system (Escherichia coli) has been assessed in a guinea pig model of acute tuberculosis. The tested antigen comprises the Mycobacterium tuberculosis (Mtb) proteins ESAT6 and CFP10 as well as modified human &ga...
Source: Acta Microbiologica et Immunologica Hungarica - February 23, 2018 Category: Microbiology Authors: Permyakova NV, Belavin PA, Pirozhkova DS, Ufimtseva EG, Rozov SM, Mursalimov SR, Sidorchuk YV, Uvarova EA, Zagorskaya AA, Marenkova TV, Bannikova SV, Demidov EA, Starostin KV, Kravchenko MA, Vakhrusheva DV, Berdnikov RB, Eremeeva NI, Skornyakov SN, Peltek Tags: Acta Microbiol Immunol Hung Source Type: research

First report of Acanthamoeba genotype T8 human keratitis.
I, Nagy ZZ Abstract Acanthamoeba has a worldwide distribution in the environment and it is capable of causing a painful sight-threatening disease of the cornea designated as Acanthamoeba keratitis (AK). Nowadays, the cases of AK have surged all over the world along with its disease burden due to increasing use of contact lenses used not only for optical correction but also for cosmetic purposes. In our present work, epithelial abrasion of a 27-year-old female soft contact lens wearer with keratitis was examined. Genotype identification was carried out with a real-time fluorescence resonance energy transfer polyme...
Source: Acta Microbiologica et Immunologica Hungarica - February 23, 2018 Category: Microbiology Authors: Orosz E, Szentmáry N, Kiss HJ, Farkas Á, Kucsera I, Nagy ZZ Tags: Acta Microbiol Immunol Hung Source Type: research

High prevalence of Staphylococcus aureus nasal carriage among children in Szolnok, Hungary.
acute;th A, Kardos S, Dobay O Abstract We collected nasal samples from 1,390 healthy 3-7 years old children in Szolnok city, Hungary, in 2012. We detected 476 Staphylococcus aureus isolates from 474 children. In two occasions, two different S. aureus were isolated, based on hemolysis type and pulsed-field gel electrophoresis pattern. S. aureus carriage rate was calculated to be 34.1% similar to others studies. Male gender was found to be a risk factor for carriage by statistical analysis. Altogether, four methicillin-resistant S. aureus (MRSA) strains were detected by mecA polymerase chain reaction, which means 0....
Source: Acta Microbiologica et Immunologica Hungarica - December 18, 2017 Category: Microbiology Authors: Laub K, Tóthpál A, Kovács E, Sahin-Tóth J, Horváth A, Kardos S, Dobay O Tags: Acta Microbiol Immunol Hung Source Type: research

Quorum-sensing network-associated gene regulation in Gram-positive bacteria.
Abstract Quorum sensing (QS) is the chemical communication processes between bacteria, which may be inter-genus or intra-genus. In general, several physiological functions, such as nutrient uptake, competence development, biofilm formation, sporulation, and toxin secretion, are accomplished through QS process. The QS (cell density-dependent process) circuit in Gram-positive bacteria consists mainly of two parts: an inducer molecule and a receptor protein. The binding of inducer molecule to receptor activates the target gene, which then performs the necessary function in bacteria. In the past few years, several inv...
Source: Acta Microbiologica et Immunologica Hungarica - December 15, 2017 Category: Microbiology Authors: Banerjee G, Ray AK Tags: Acta Microbiol Immunol Hung Source Type: research

Does subinhibitory concentrations of clinically important antibiotic induce biofilm production of Enterococcus faecium strains?
Abstract Biofilm structures are the most resistant form of active microorganisms against sanitation, disinfection, and sterilization processes. One of the specific properties of biofilm is the development of antibiotic resistance that can be up to 1,000-fold greater than planktonic cells. Enterococcus faecium is a human pathogen that causes nosocomial bacteremia and at the present time, it is well known that most of the chronic infections are biofilm-based. Recent evidence suggested that subinhibitory concentrations (sub-MICs) of antibiotics have an important role in the evolution of antibiotic resistance and indu...
Source: Acta Microbiologica et Immunologica Hungarica - December 15, 2017 Category: Microbiology Authors: Yuksel FN, Karatug NT, Akcelik M Tags: Acta Microbiol Immunol Hung Source Type: research

The recombinant fusion protein CFP10-ESAT6-dIFN has protective effect against tuberculosis in guinea pigs.
SE, Deineko EV Abstract Development of effective vaccine candidates against tuberculosis (TB) is currently the most important challenge in the prevention of this disease since the BCG vaccine fails to guarantee a lifelong protection, while any other approved vaccine with better efficiency is still absent. The protective effect of the recombinant fusion protein CFP10-ESAT6-dIFN produced in a prokaryotic expression system (Escherichia coli) has been assessed in a guinea pig model of acute TB. The tested antigen comprises the Mycobacterium tuberculosis (Mtb) proteins ESAT6 and CFP10 as well as modified human γ-...
Source: Acta Microbiologica et Immunologica Hungarica - December 15, 2017 Category: Microbiology Authors: Permyakova NV, Belavin PA, Pirozhkova DS, Ufimtseva EG, Rozov SM, Mursalimov SR, Sidorchuk YV, Uvarova EA, Zagorskaya AA, Marenkova TV, Bannikova SV, Demidov EA, Starostin KV, Kravchenko MA, Vakhrusheva DV, Berdnikov RB, Eremeeva NI, Skornyakov SN, Peltek Tags: Acta Microbiol Immunol Hung Source Type: research

Faecal microbiota transplantation for Clostridium difficile infection using a lyophilized inoculum from non-related donors: A case series involving 19 patients.
In this study, our objective was to evaluate the rate of resolution of diarrhea following administration of lyophilized and resolved FMT via a nasogastric (NG) tube. We recruited 19 patients suffered from laboratory-confirmed C. difficile infection. Each of them was treated by lyophilized and resolved inoculum through a NG tube. One participant succumbed following the procedure due to unrelated diseases. Out of 18 cases, 15 patients reportedly experienced a resolution of the symptoms. One patient was treated with another course of antibiotics, and two of the non-responders were successfully retreated with another course of...
Source: Acta Microbiologica et Immunologica Hungarica - December 14, 2017 Category: Microbiology Authors: Vigvári S, Sipos D, Solt J, Vincze Á, Kocsis B, Nemes Z, Kappéter Á, Feiszt Z, Kovács B, Péterfi Z Tags: Acta Microbiol Immunol Hung Source Type: research

Sequence analysis of Schmallenberg virus genomes detected in Hungary.
In this study, we determined the complete coding sequence of seven Hungarian SBV genomes to expand our knowledge about the genetic diversity of circulating field strains. The samples originated from the first case, an aborted cattle fetus without malformation collected in 2012, and from the blood samples of six adult cattle in 2014. The Hungarian SBV sequences shared ≥99.3% nucleotide (nt) and ≥97.8% amino acid (aa) identity with each other, and ≥98.9 nt and ≥96.7% aa identity with reference strains. Although phylogenetic analyses showed low resolution in general, the M sequences of cattle and sheep origin SBV ...
Source: Acta Microbiologica et Immunologica Hungarica - December 14, 2017 Category: Microbiology Authors: Fehér E, Marton S, Tóth ÁG, Ursu K, Wernike K, Beer M, Dán Á, Bányai K Tags: Acta Microbiol Immunol Hung Source Type: research

Characterization of the Rhodococcus sp. MK1 strain and its pilot application for bioremediation of diesel oil-contaminated soil.
e;cs T, Rákhely G, Perei K Abstract Petroleum hydrocarbons and derivatives are widespread contaminants in both aquifers and soil, their elimination is in the primary focus of environmental studies. Microorganisms are key components in biological removal of pollutants. Strains capable to utilize hydrocarbons usually appear at the contaminated sites, but their metabolic activities are often restricted by the lack of nutrients and/or they can only utilize one or two components of a mixture. We isolated a novel Rhodococcus sp. MK1 strain capable to degrade the components of diesel oil simultaneously. The draft ...
Source: Acta Microbiologica et Immunologica Hungarica - December 6, 2017 Category: Microbiology Authors: Kis ÁE, Laczi K, Zsíros S, Kós P, Tengölics R, Bounedjoum N, Kovács T, Rákhely G, Perei K Tags: Acta Microbiol Immunol Hung Source Type: research

Aflatoxin B1 detoxification by cell-free  extracts of Rhodococcus strains.
Aflatoxin B1 detoxification by cell-free extracts of Rhodococcus strains. Acta Microbiol Immunol Hung. 2017 Dec 01;:1-16 Authors: Risa A, Divinyi DM, Baka E, Krifaton C Abstract Aflatoxin B1 (AFB1) produced by Aspergillus molds is a genotoxic and carcinogenic mycotoxin. For the elimination of mycotoxins from food and feed, biodetoxification can be a successful tool. The aim of this study was to reveal biodetoxification with the cell-free extracts of Rhodococcus erythropolis NI1 and Rhodococcus rhodochrous NI2, which have been already proved to detoxify AFB1. Extracellular matrices of cultures and...
Source: Acta Microbiologica et Immunologica Hungarica - December 1, 2017 Category: Microbiology Authors: Risa A, Divinyi DM, Baka E, Krifaton C Tags: Acta Microbiol Immunol Hung Source Type: research

Emergence of fosfomycin resistance among isolates of Escherichia coli harboring extended-spectrum and AmpC β-lactamases.
This study reports the first detection of fosfomycin resistance in Iran. This study indicated the increasing prevalence of UTI isolates of E. coli-harboring ESBL and AmpC β-lactamases genes in Iran. Therefore, due to the high rate of blaDHA and blaCMY genes and emergence of fosfomycin-resistant E. coli isolates, we recommend continuous monitoring of antibiotic resistance as well as attention to guidelines of infection controls. PMID: 29137487 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - November 15, 2017 Category: Microbiology Authors: Bahramian A, Eslami G, Hashemi A, Tabibi A, Heidary M Tags: Acta Microbiol Immunol Hung Source Type: research

Abstracts of the 26th Semmelweis Symposium 2017 - New Challenges in Microbiology, 9-10 November, 2017 Budapest, Hungary.
Authors: PMID: 29106799 [PubMed] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - November 1, 2017 Category: Microbiology Tags: Acta Microbiol Immunol Hung Source Type: research

Abstracts of the Annual Meeting of the Hungarian Society for Microbiology, October 19-21, 2016, Keszthely, Hungary.
Authors: PMID: 29106802 [PubMed] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - November 1, 2017 Category: Microbiology Tags: Acta Microbiol Immunol Hung Source Type: research

Abstracts Of the 5th Central European Forum for Microbiology, October 18-20, 2017, Keszthely, Hungary.
Authors: PMID: 29106803 [PubMed] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - November 1, 2017 Category: Microbiology Tags: Acta Microbiol Immunol Hung Source Type: research

Occurrence of vancomycin-resistant Staphylococcus aureus in the oral cavity of patients with dental caries.
Abstract Oral streptococci are the major group of microbes isolated from oral microflora. They represent frequent pathogens of infective endocarditis (IE), and it is assumed that in most of the cases oral streptococci are acquired via mucosa layer of oral cavity. Staphylococcus aureus is also frequently isolated from IE as it accounts for 20%-30% of all cases. Vancomycin has been the most reliable therapeutic agent against infections caused by methicillin-resistant S. aureus (MRSA). The main objective of this study was to examine the occurrence of S. aureus species in dental caries specimens. Antimicrobial suscept...
Source: Acta Microbiologica et Immunologica Hungarica - September 11, 2017 Category: Microbiology Authors: Vellappally S, Divakar DD, Al Kheraif AA, Ramakrishnaiah R, Alqahtani A, Dalati MHN, Anil S, Khan AA, Harikrishna Varma PR Tags: Acta Microbiol Immunol Hung Source Type: research

Resurgence of yellow fever in Brazil: Overview and possible control options.
PMID: 28889757 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - September 11, 2017 Category: Microbiology Authors: Khan AW, Nasim Z, Zahir F, Khan MA, Ali A, Ali S, Khan AH Tags: Acta Microbiol Immunol Hung Source Type: research

MALDI-TOF MS versus 16S rrna sequencing: Minor discrepancy between tools in identification of Bacteroides isolates.
te;czy K, Melegh SZ, Urbán E Abstract Members of the genus Bacteroides are important components of the normal microbiota of gastrointestinal tract; however, as opportunistic pathogens are also associated with severe or even life-threatening infections with significant mortality. Various species within Bacteroides fragilis group are phenotypically very similar; thus, their identifications with traditional-automated biochemical methods are frequently inaccurate. The identification of the newly discovered or reclassified bacteria can be doubtful because of the lack of biochemical profile in the database of the...
Source: Acta Microbiologica et Immunologica Hungarica - September 11, 2017 Category: Microbiology Authors: Sárvári KP, Sóki J, Iván M, Miszti C, Latkóczy K, Melegh SZ, Urbán E Tags: Acta Microbiol Immunol Hung Source Type: research

Efficiency of temporary storage of geothermal waters in a lake system: Monitoring the changes of water quality and bacterial community structures.
In this study, the physical and chemical properties and changes in the bacterial community structure of a reservoir lake system in southeast region of Hungary were monitored and compared through 2 years, respectively. The values of biological oxygen demand, concentrations of ammonium ion, total inorganic nitrogen, total phosphorous, and total phenol decreased, whereas oxygen saturation, total organic nitrogen, pH, and conductivity increased during the storage period. Bacterial community structure of water and sediment samples was compared by denaturing gradient gel electrophoresis (DGGE) following the amplification of the ...
Source: Acta Microbiologica et Immunologica Hungarica - September 5, 2017 Category: Microbiology Authors: Szirányi B, Krett G, Kosáros T, Janurik E, Pekár F, Márialigeti K, Borsodi AK Tags: Acta Microbiol Immunol Hung Source Type: research

High prevalence of OXA-type carbapenemases among Acinetobacter baumannii strains in a teaching hospital of Tehran.
In this study, 100 isolates of CRAB from hospitalized patients during 2015-2016 at Imam Khomeini Hospital were investigated to determine the rates of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains using Kirby-Bauer disk diffusion method. The minimum inhibitory concentrations (MICs) of six antibiotics were determined by broth microdilution method. Multiplex polymerase chain reaction (PCR) was performed to detect blaOXA-51 like and blaOXA-58 like, blaOXA-23 like, and blaOXA-24 like that are encoding resistance to carbapenems. All CRAB isolates were MDR and XDR and 2% of the...
Source: Acta Microbiologica et Immunologica Hungarica - September 5, 2017 Category: Microbiology Authors: Zafari M, Feizabadi MM, Jafari S, Abdollahi A, Sabokbar A Tags: Acta Microbiol Immunol Hung Source Type: research

Emergence of armA and rmtB genes among VIM, NDM, and IMP metallo- β-lactamase-producing multidrug-resistant Gram-negative pathogens.
This study was aimed to establish the pattern of antibiotic sensitivity and relevance of antimicrobial resistance in aerobic Gram-negative bacilli. A total of 103 aerobic Gram-negative bacteria namely Escherichia coli, Klebsiella pneumoniae, Enterobacter spp., Citrobacter koserii, Proteus spp., and Pseudomonas aeruginosa were collected from tertiary care centers around Chennai. Kirby-Bauer Disk Diffusion test and study for genes of cephalosporin, carbapenem, and aminoglycoside resistance were done. A descriptive analysis of the data on altogether 103 clinical urine isolates was performed. All strains showed susceptibility ...
Source: Acta Microbiologica et Immunologica Hungarica - September 5, 2017 Category: Microbiology Authors: Gopalakrishnan S, Kamalanathan A, Rajan S, Bhagat VM, Ali MKS Tags: Acta Microbiol Immunol Hung Source Type: research

First report of coexistence of AmpC beta-lactamase genes in Klebsiella pneumoniae strains isolated from burn patients.
In conclusion, K. pneumoniae is becoming a serious problem in burn patients. Accurate and precise methods and guidelines should be designed for detection of antibiotic-resistant mechanisms. Our data showed the high rate of AmpC beta-lactamase among K. pneumoniae isolated from burn patients, which limit the treatment options. Therefore, the results of this study can provide evidence to help for appropriate treatment of burn patients. PMID: 28859497 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2017 Category: Microbiology Authors: Ghanavati R, Darban-Sarokhalil D, Navab-Moghadam F, Kazemian H, Irajian G, Razavi S Tags: Acta Microbiol Immunol Hung Source Type: research

Influence of human milk oligosaccharides on adherence of bifidobacteria and clostridia to cell lines.
Abstract Adhesion of gut bacteria to the intestinal epithelium is the first step in their colonization of the neonatal immature gut. Bacterial colonization of the infant gut is influenced by several factors, of which the most important are the mode of delivery and breast-feeding. Breast-fed infants ingest several grams of human milk oligosaccharides (HMOs) per day, which can become receptor decoys for intestinal bacteria. The most abundant intestinal bacteria in vaginally delivered infants are bifidobacteria, whereas infants born by cesarean section are colonized by clostridia. The influence of HMOs on the adhesio...
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2017 Category: Microbiology Authors: Musilova S, Modrackova N, Doskocil I, Svejstil R, Rada V Tags: Acta Microbiol Immunol Hung Source Type: research

Prevalence and molecular typing of methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin gene.
Abstract Panton-Valentine leukocidin (pvl) toxin is an important virulence factor of Staphylococcus aureus. The main genes are coa and spa for distinguishing and typing of S. aureus isolates. The aim of this study was to investigate antibiotic resistance, presence of mecA and pvl genes, as well as epidemiological typing of these isolates according to polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in clinical sample isolated from Rasht city, Iran. A total of 250 clinical samples have been isolated from different hospitals. First, isolates of S. aureus were identified through mi...
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2017 Category: Microbiology Authors: Hesari MR, Salehzadeh A, Darsanaki RK Tags: Acta Microbiol Immunol Hung Source Type: research

Emergence of NDM-1 among carbapenem-resistant Klebsiella pneumoniae in Iraqi hospitals.
This study reports NDM-1 for the first time among Klebsiella pneumoniae from hospitalized patients in Baghdad, Iraq. Fifty-five clinical isolates of K. pneumoniae resistant to carbapenem were investigated from burned wounds, sputum, and blood samples. The susceptibility to different antibiotics was tested by VITEK-2 system. All strains were multidrug-resistant and they showed nine different antimicrobial-resistant patterns (A-I) and the most effective antibiotic on these strains was levofloxacin (85.45%). The phenotypic detection of carbapenemases by MASTDISCS D70C revealed 29 (52.73%) strains were MBL-producing, out of 55...
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2017 Category: Microbiology Authors: Hussein NH Tags: Acta Microbiol Immunol Hung Source Type: research

Is there a hormonal regulation of phagocytosis at unicellular and multicellular levels? A critical review.
Abstract Phagocytosis is an ancient cell function, which is similar at unicellular and multicellular levels. Unicells synthesize, store, and secrete multicellular (mammalian) hormones, which influence their phagocytosis. Amino acid hormones, such as histamine, serotonin, epinephrine, and melatonin stimulate phagocytosis, whereas peptide hormones, such as adrenocorticotropic hormone (ACTH), insulin, opioids, arginine vasopressin, and atrial natriuretic peptide decreased it, independently on their chemical structure or function in multicellulars. Macrophage phagocytosis of multicellulars is also stimulated by amino ...
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2017 Category: Microbiology Authors: Csaba G Tags: Acta Microbiol Immunol Hung Source Type: research

High frequency of methicillin-resistant Staphylococcus aureus (MRSA) with SCCmec type III and spa type t030 in Karaj's teaching hospitals, Iran.
In conclusion, high frequency of spa t030 with SCCmec type III and MRSA phenotype illustrated circulating of one of the antibiotic-resistant strains in ICU of Karaj's teaching hospitals and emphasizes the need for ongoing molecular surveillance, antibiotic susceptibility monitoring, and infection control. PMID: 28836817 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - August 24, 2017 Category: Microbiology Authors: Bayat B, Zade MH, Mansouri S, Kalantar E, Kabir K, Zahmatkesh E, Sepehr MN, Naseri MH, Darban-Sarokhalil D Tags: Acta Microbiol Immunol Hung Source Type: research

Colistin resistance associated with outer membrane protein change in  Klebsiella pneumoniae and Enterobacter asburiae.
This study demonstrated that OMP differences were between colistin-susceptible and -resistant counterpart strains. The altered Gram-negative cell wall may contribute to acquired colistin resistance in Enterobacteriaceae. PMID: 28629229 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - June 20, 2017 Category: Microbiology Authors: Kádár B, Kocsis B, Tóth Á, Kristóf K, Felső P, Kocsis B, Böddi K, Szabó D Tags: Acta Microbiol Immunol Hung Source Type: research

Association between Toxoplasma gondii types and outcomes of human infection: A meta-analysis.
Abstract The virulence and pathogenicity of various types of Toxoplasma gondii differ considerably in mice. Recent studies have claimed that similar phenomenon was observed in humans, but no relevant studies have been performed to validate this finding. In addition, reports showing association between a given T. gondii type and outcomes of human infection yielded conflicting results. To provide a more precise estimation of the association and a more reliable conclusion on this subject, we performed this meta-analysis. Relevant literatures were identified in multiple databases and selected based on strict screening...
Source: Acta Microbiologica et Immunologica Hungarica - June 20, 2017 Category: Microbiology Authors: Xia J, Cheng XY, Wang XJ, Peng HJ Tags: Acta Microbiol Immunol Hung Source Type: research