Time-variable expression levels of mazF, atlE, sdrH, and bap genes during biofilm formation in Staphylococcus epidermidis.
In this study, a catheter-derived S. epidermidis isolate with strong ability of biofilm formation was selected. PCR assay was used to detect sdrH, bap, atlE, and mazF genes in this isolate. Real-time PCR was used to determine the expression levels of these genes after 4, 8, and 20 h during the course of biofilm formation. The studied genes showed different expression levels at different time intervals during biofilm formation by real-time PCR method. Expression levels of atlE and sdrH genes were the highest at 4 h, whereas bap gene showed the highest expression level at 8 h during the course of biofilm forma...
Source: Acta Microbiologica et Immunologica Hungarica - June 14, 2019 Category: Microbiology Authors: Shivaee A, Mohammadzadeh R, Shahbazi S, Pardakhtchi E, Ohadi E, Kalani BS Tags: Acta Microbiol Immunol Hung Source Type: research

Methicillin-resistant Staphylococcus aureus colonization in HIV patients of Arba Minch province, Ethiopia: Carriage rates, antibiotic resistance, and biofilm formation.
This study aimed at delineating the prevalence, antimicrobial resistance, and biofilm-forming potentials of nasally colonized MRSA among HIV patients in the Arba Minch province of Ethiopia. A cross-sectional study was performed in HIV patients who visit anti-retroviral therapy clinic of the Arba Minch Hospital between February and April 2017. Nasal samples were collected and inspected for Staphylococcus following standard procedures. MRSA was identified using cefoxitin disk and antibiotics sensitivity test was performed as per Kirby-Baur disk diffusion method. The formation of biofilm was inspected using both qualitative a...
Source: Acta Microbiologica et Immunologica Hungarica - June 14, 2019 Category: Microbiology Authors: Manilal A, Shewangizaw M, Mama M, Gezmu T, Merdekios B Tags: Acta Microbiol Immunol Hung Source Type: research

Phylogenetic analysis of human bocavirus in children with acute respiratory infections in Iran.
This study was carried out in order to analyze the infection frequency and coinfection rates of HBoV with respiratory syncytial virus (RSV) and to perform phylogenetic analysis of HBoV in samples of children with acute respiratory infection in Isfahan, Iran. During the time period 2016-2017, altogether 75 respiratory samples from children hospitalized with acute respiratory infection were collected. The samples were first screened for RSV by direct immunofluorescence method and then subjected to detect HBoV DNA by PCR. Genotyping of HBoV-positive samples was conducted by direct sequencing of PCR products using NP and VP1/V...
Source: Acta Microbiologica et Immunologica Hungarica - May 31, 2019 Category: Microbiology Authors: Mohammadi M, Yavarian J, Karbasizade V, Moghim S, Esfahani BN, Hosseini NS Tags: Acta Microbiol Immunol Hung Source Type: research

Diagnosis of malaria in a traveler 9 months after returning from West Africa by illumigene ® LAMP assay: A case report.
We report a 53-year-old male patient who was hospitalized with fever attacks, chills, and headache caused 9 months after returning from Africa. During his stay in Africa, he used malaria chemoprophylaxis. Microscopy of thin and thick blood films and rapid diagnostic antigen testing remained negative for three times. The EDTA blood samples were tested using the Meridian illumigene® malaria LAMP assay that gave a positive result for Plasmodium spp. Diagnosis of malaria was subsequently specified as P. ovale infection by real-time PCR. Ovale malaria often manifests with delay and low parasitemia. The patient was treated w...
Source: Acta Microbiologica et Immunologica Hungarica - May 28, 2019 Category: Microbiology Authors: Reuken PA, Baier M, Hagel S, Eisermann P, Stallmach A, Rödel J Tags: Acta Microbiol Immunol Hung Source Type: research

Clinical pitfalls of leishmaniasis and Whipple's disease hidden behind systemic lupus erythematosus: A case series.
Abstract Systemic lupus erythematosus (SLE) is a multisystemic autoimmune disease that can affect major organs possibly leading to life-threatening complications and appears with heterogeneous clinical picture. SLE could present with broad spectrum of clinical and laboratory features that can resemble those of other diseases, such as hemopoietic malignancies, infections, or immune-mediated disorders. Its complexity and protean features overlap with many other diseases, hindering the differential diagnosis. Rarely, true overlap with other diseases may occur. Herein, we report a case series of two patients affected ...
Source: Acta Microbiologica et Immunologica Hungarica - May 17, 2019 Category: Microbiology Authors: Francesca L, Firinu D, Deidda M, Costanzo G, Del Giacco SR Tags: Acta Microbiol Immunol Hung Source Type: research

Helicobacter pylori genotypes among Belarus patients with gastroduodenal disorders and their association with clinical outcome.
Abstract The aim of this study was to evaluate the prevalence of Helicobacter pylori genotypes (vacA and cagPAI) directly in gastric biopsy specimens in patients with gastric diseases in Belarus. Gastric biopsies were collected from 461 patients with different gastrointestinal disorders: superficial gastritis (287 subjects), atrophy gastritis (59 subjects), erosive gastritis (47 subjects), duodenal ulcer disease (54 subjects), and stomach ulcer (14 subjects). PCR-based genotyping was used to detect s1a, s1b, s2, m1a, m1b, m2, cagM, cagA, and cagT genes. Overall prevalence of vacA s1a allele was 60.5% followed by m...
Source: Acta Microbiologica et Immunologica Hungarica - May 17, 2019 Category: Microbiology Authors: Yanovich O, Doroshko M, Titov L Tags: Acta Microbiol Immunol Hung Source Type: research

Distribution of genes encoding resistance to macrolides, lincosamides, and streptogramins among methicillin-resistant Staphylococcus aureus strains isolated from burn patients.
This study aimed to investigate the prevalence of ermA, ermB, ermC, and msrA in MRSA strains isolated from burn patients in Ahvaz, southwest of Iran. A total of 76 isolates of S. aureus were collected from January to May 2017 from Taleghani Burn Hospital in Ahvaz. Among 76 S. aureus strains collected, 60 (78.9%) isolates were MRSA. The antimicrobial susceptibility testing for MRSA showed extreme high resistance rate to clarithromycin (100%) and azithromycin (100%), followed by erythromycin (98.3%). The PCR assay revealed that the frequency rates of msrA, ermA, and ermC genes were 23 (38.3%), 28 (46.7%), and 22 (36.7%), res...
Source: Acta Microbiologica et Immunologica Hungarica - May 17, 2019 Category: Microbiology Authors: Khoshnood S, Shahi F, Jomehzadeh N, Montazeri EA, Saki M, Mortazavi SM, Maghsoumi-Norouzabad L Tags: Acta Microbiol Immunol Hung Source Type: research

Identification of an intestinal microbiota signature associated with hospitalized patients with diarrhea.
Abstract As an important global health challenge, diarrhea kills nearly two million people each year. Postinfectious irritable bowel syndrome (IBS) usually manifests itself as the diarrhea-predominant subtype. Small intestinal bacterial overgrowth has been observed more frequently in patients with IBS compared to healthy controls. However, the pathophysiology of IBS is not fully understood, and based on recent evidences, altered gut microbiota is involved in the pathogenesis of IBS. Therefore, we aimed to compare the microbiome in hospitalized patients with diarrhea and healthy individuals. Thirty patients and 10 ...
Source: Acta Microbiologica et Immunologica Hungarica - May 7, 2019 Category: Microbiology Authors: Mohammadzadeh N, Kalani BS, Bolori S, Azadegan A, Gholami A, Mohammadzadeh R, Masjedian Jazi F Tags: Acta Microbiol Immunol Hung Source Type: research

Clinical course of Acanthamoeba keratitis by genotypes T4 and T8 in Hungary.
This study reports seven CL wearer, Acanthamoeba PCR-positive patients with AK, diagnosed between January 2015 and 2018. Patients had the diagnosis of AK 1.36 months after first symptoms. Genotyping allowed the identification of six isolates of the T4 and one of the T8 genotypes. At first presentation, pseudendritiformic epithelopathy/dirty epithelium (four eyes, 57.1%), multifocal stromal infiltrates (five eyes, 71.4%), ring infiltrate (three eyes, 42.8%), and perineuritis (one eye, 14.3%) were observed. AK was healed without later recurrence in two eyes (28.5%) using triple-topical therapy, in three eyes (42.8%) followin...
Source: Acta Microbiologica et Immunologica Hungarica - March 5, 2019 Category: Microbiology Authors: Orosz E, Kriskó D, Shi L, Sándor GL, Kiss HJ, Seitz B, Nagy ZZ, Szentmáry N Tags: Acta Microbiol Immunol Hung Source Type: research

Detection of Acanthamoeba spp. from dust phenomenon in Ilam Province, West Iran.
This study aimed to represent the existence and genotyping of Acanthamoeba spp. in dust phenomenon in Ilam Province, Iran. In this study, 55 dust samples were collected and by targeting the diagnostic fragment 3 region of the 18S rRNA gene, the genotypes were determined. Utilizing the tolerance ability test, the pathogenic potential of all positive isolates was also recognized. Eighteen samples of Acanthamoeba (32.7%) were detected in the sampling areas. According to sequencing analysis, the isolates related to T4 (77.7%) and T2 (22.3%) genotypes were reported. It was revealed by thermo- and osmotolerance tests in which si...
Source: Acta Microbiologica et Immunologica Hungarica - March 5, 2019 Category: Microbiology Authors: Saberi R, Najafi A, Naserifar R Tags: Acta Microbiol Immunol Hung Source Type: research

A powerful in vivo alternative model in scientific research: Galleria mellonella.
Abstract Murine models are suggested as the gold standard for scientific research, but they have many limitations of ethical and logistical concern. Then, the alternative host models have been developed to use in many aspects especially in invertebrate animals. These models are selected for many areas of research including genetics, physiology, biochemistry, evolution, disease, neurobiology, and behavior. During the past decade, Galleria mellonella has been used for several medical and scientific researches focusing on human pathogens. This model commonly used their larvae stage due to their easy to use, non-essen...
Source: Acta Microbiologica et Immunologica Hungarica - February 28, 2019 Category: Microbiology Authors: Singkum P, Suwanmanee S, Pumeesat P, Luplertlop N Tags: Acta Microbiol Immunol Hung Source Type: research

First isolation of carbapenem-resistant Acinetobacter beijerinckii from an environmental sample.
lay S Abstract The emergence of opportunistic Acinetobacter spp. in healthcare settings poses a significant threat to public health. The major reasons for nosocomial spread of these species are their abilities to develop and transfer drug resistance against various classes of antibiotics. Considering that Acinetobacter spp. are ubiquitous in nature, can utilize several carbon sources, and reach humans via various pathways, our aim was to obtain information about the environmental strains of this genus. Our first step was to develop and test a multistep isolation procedure based on traditional scientific methods. A...
Source: Acta Microbiologica et Immunologica Hungarica - February 28, 2019 Category: Microbiology Authors: Radó J, Kaszab E, Benedek T, Kriszt B, Szoboszlay S Tags: Acta Microbiol Immunol Hung Source Type: research

Antibiotic resistance, virulence and genetic diversity of Klebsiella pneumoniae in community- and hospital-acquired urinary tract infections in Iran.
In this study, 91.7%, 33.3%, and 4.2% of phenotypically ESBL-producers were positive for blaCTX-M, blaTEM, and blaSHV genes, respectively. An association was observed between the presence of traT, fyuA, or cnf-1 genes with antibiotic resistance. Two clone types were obtained by PFGE that indicate different K. pneumoniae clones in community- and hospital-acquired UTIs. The findings of this study are valuable in development of treatment strategies against UTIs in Iran. PMID: 30803251 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - February 26, 2019 Category: Microbiology Authors: Eghbalpoor F, Habibi M, Azizi O, Asadi Karam MR, Bouzari S Tags: Acta Microbiol Immunol Hung Source Type: research

Next-generation sequencing of plasmid carrying blaOXA-48 in Klebsiella pneumoniae from Turkey.
l;, Saral A Abstract A carbapenem-resistant Klebsiella pneumoniae strain was isolated in Turkey in 2012 and blaNDM-1 and blaOXA-48 genes were observed in this strain. The aim of this study was to investigate transferability of plasmid bearing blaOXA-48 in K. pneumoniae and to use whole-genome sequencing in order to understand the genetic context of plasmid. K. pneumoniae strain was used as donor in conjugation experiments. Antibiotic susceptibility profile of selected transconjugant was determined. Plasmid was isolated from transconjugant colony and was named as pKPT. Complete sequencing of the pKPT was conducted ...
Source: Acta Microbiologica et Immunologica Hungarica - February 26, 2019 Category: Microbiology Authors: Düzgün AÖ, Saral A Tags: Acta Microbiol Immunol Hung Source Type: research

Aromatic hydrocarbon receptors in the immune system: Review and hypotheses.
Abstract Ah-receptors (AhRs) recognize and bind foreign environmental molecules as well as some target hormones of other nuclear receptors. As ligands activate transcription factors, they transmit the information on the presence of these molecules by binding to the DNA, which in turn activate xenobiotic metabolism genes. Cross talk with other nuclear receptors or some non-nuclear receptors also activates or inhibits endocrine processes. Immune cells have AhRs by which they are activated for physiological (immunity) or non-physiological (allergy and autoimmunity) processes. They can be imprinted by hormonal or pseu...
Source: Acta Microbiologica et Immunologica Hungarica - February 26, 2019 Category: Microbiology Authors: Csaba G Tags: Acta Microbiol Immunol Hung Source Type: research

Aminoglycoside resistance determinants in multiresistant Escherichia coli and Klebsiella pneumoniae clinical isolates from Turkish and Syrian patients.
e;nez-Martínez L Abstract Escherichia coli and Klebsiella pneumoniae are frequently found resistance to aminoglycosides in Turkey. The aim of this study was to investigate aminoglycoside resistance in clinical isolates of E. coli and K. pneumoniae from Turkey using both phenotypic and genotypic methods and screening for the prevalence of gene coding for common aminoglycoside-modifying enzymes (AMEs) and 16S rRNA methylase genes. A total of 88 consecutive, non-duplicated E. coli (n = 65) and K. pneumoniae (n = 23) isolates showing resistance or intermediate resistance to amikacin ...
Source: Acta Microbiologica et Immunologica Hungarica - February 26, 2019 Category: Microbiology Authors: Cirit OS, Fernández-Martínez M, Yayla B, Martínez-Martínez L Tags: Acta Microbiol Immunol Hung Source Type: research

High incidence of multiresistant bacterial isolates from bloodstream infections in trauma emergency department and intensive care unit in Serbia.
Abstract We investigated the incidence of bloodstream infections (BSIs) in trauma emergency department (ED) and intensive care unit (ICU), to assess ED- and ICU-related predictors of BSI and to describe the most common bacteria causing BSI and their antimicrobial resistance markers. A prospective study was conducted in two trauma ICUs of the ED of Clinical Center of Serbia. Overall, 62 BSIs were diagnosed in 406 patients, of which 13 were catheter-related BSI (3.0/1,000 CVC-days) and 30 BSIs of unknown origin, while 15% were attributed to ED CVC exposure. Lactate ≥2 mmol/L and SOFA score were independent E...
Source: Acta Microbiologica et Immunologica Hungarica - February 21, 2019 Category: Microbiology Authors: Djuric O, Markovic-Denic L, Jovanovic B, Bumbasirevic V Tags: Acta Microbiol Immunol Hung Source Type: research

High distribution of 16S rRNA methylase genes rmtB and armA among Enterobacter cloacae strains isolated from an Ahvaz teaching hospital, Iran.
This study aimed to investigate the prevalence of 16S rRNA methylases among Enterobacter cloacae strains isolated from an Ahvaz teaching hospital, Iran. A total of 68 E. cloacae clinical strains were collected between November 2017 and September 2018. The MICs of aminoglycosides were assessed using the agar dilution method. The presence of 16S rRNA methylase genes, including armA, rmtA to rmtH, and nmpA was evaluated by PCR. The transferability of 16S rRNA methylase-harboring plasmids was evaluated by conjugation assay. The genetic diversity of all isolates was evaluated by ERIC-PCR. The armA and rmtB genes were the only 1...
Source: Acta Microbiologica et Immunologica Hungarica - February 21, 2019 Category: Microbiology Authors: Amin M, Mehdipour G, Navidifar T Tags: Acta Microbiol Immunol Hung Source Type: research

MRSA diversity and the emergence of LA-MRSA in a large teaching hospital in Slovenia.
Abstract The methicillin-resistant Staphylococcus aureus (MRSA) is one of the major causes of a variety of infections in hospitals and the community. One of the most prominent changes in the MRSA epidemiology is the emergence of livestock-associated MRSA (LA-MRSA) strains in the human population. The aim of this study was to follow the MRSA epidemiology in a large teaching hospital during an 8-year time period (2006-2013). Altogether 519 MRSA, cultured from screening or clinical samples, were distributed into 77 spa types, of which three (t003 and t001, associated with CC5; and t015; associated with CC45) were the...
Source: Acta Microbiologica et Immunologica Hungarica - January 25, 2019 Category: Microbiology Authors: Kevorkijan BK, Petrovič Ž, Kocuvan A, Rupnik M Tags: Acta Microbiol Immunol Hung Source Type: research

Prevalence of Helicobacter pylori is still high among symptomatic Bulgarian children.
In conclusion, H. pylori infection is still an important concern for Bulgarian children, although having decreased by about 1.8%/yearly over 21 years. This study reveals the importance of H. pylori diagnostics even in the youngest symptomatic children and demonstrates an inverse association between either GERD or bile reflux and H. pylori infection. PMID: 30585499 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - December 26, 2018 Category: Microbiology Authors: Boyanova L, Hadzhiyski P, Markovska R, Yaneva P, Yordanov D, Gergova G, Mitov I Tags: Acta Microbiol Immunol Hung Source Type: research

Experiences with fecal microbiota transplantation in Clostridium difficile infections via upper gastrointestinal tract.
In conclusion, FMT proved to be very effective, particularly in recurrent infections and in cases where conventional treatment had failed. PMID: 30585500 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - December 26, 2018 Category: Microbiology Authors: Vigvári S, Vincze Á, Solt J, Sipos D, Feiszt Z, Kovács B, Kappéter Á, Péterfi Z Tags: Acta Microbiol Immunol Hung Source Type: research

In Memoriam Professor Piroska Anderlik (1935-2018).
te; D PMID: 30565475 [PubMed - in process] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - December 1, 2018 Category: Microbiology Authors: Szabó D Tags: Acta Microbiol Immunol Hung Source Type: research

Immune responses elicited by the recombinant Erp, HspR, LppX, MmaA4, and OmpA proteins from Mycobacterium tuberculosis in mice.
This study showed that MmaA4, HspR, and Erp proteins from MTB are successful in eliciting both humoral and cellular immune responses in mice. PMID: 30484328 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - November 28, 2018 Category: Microbiology Authors: Okay S, Çetin R, Karabulut F, Doğan C, Sürücüoğlu S, Kızıldoğan AK Tags: Acta Microbiol Immunol Hung Source Type: research

Plasmid copy number and qnr gene expression in selection of fluoroquinolone-resistant Escherichia coli.
In this study, we studied Escherichia coli strains, each carried one plasmid-mediated quinolone resistance determinant namely, qnrA1, qnrB1, qnrC1, and qnrD1. We exposed 0.5 McFarland density of each strain to 0.5 mg/L ciprofloxacin from the period of 30, 60, 90, and 120 min over 24 h. All treated strains were further exposed to a constantly increasing 1, 2, 4, and 8 mg/L ciprofloxacin solution through 24, 48, and 120 h. In given timepoints, RNA was extracted from all treated strains. Expression of qnrA1, qnrB1, qnrC1, and qnrD1 was investigated by quantitative PCR. Mutations in gyrA and parC g...
Source: Acta Microbiologica et Immunologica Hungarica - November 22, 2018 Category: Microbiology Authors: Gulyás D, Kocsis B, Szabó D Tags: Acta Microbiol Immunol Hung Source Type: research

High prevalence of vancomycin and high-level gentamicin resistance in Enterococcus faecalis isolates.
Abstract Multiple drug-resistant enterococci are major cause of healthcare-associated infections due to their antibiotic resistance traits. Among them, Enterococcus faecalis is an important opportunistic pathogen causing various hospital-acquired infections. A total of 53 E. faecalis isolates were obtained from various infections. They were identified by phenotypic and genotypic methods. Determination of antimicrobial resistance patterns was done according to CLSI guidelines. The isolates that were non-susceptible to at least one agent in ≥3 antimicrobial categories were defined as multidrug-resistant (MDR). De...
Source: Acta Microbiologica et Immunologica Hungarica - November 22, 2018 Category: Microbiology Authors: Taji A, Heidari H, Ebrahim-Saraie HS, Sarvari J, Motamedifar M Tags: Acta Microbiol Immunol Hung Source Type: research

Changes in mRNA expression of members of TGFB1-associated pathways in human leukocytes during EBV infection.
Abstract Transforming growth factor β 1 (TGFB1) likely contributes to the pathogenesis of Epstein-Barr virus (EBV)-mediated cancer. A microarray containing 59 probes for detecting mRNA of members of TGFB1-associated pathways was developed. mRNA expression of TGFB1 receptors and members of connected pathways were examined in peripheral blood leukocytes of patients during acute EBV infection and after recovery. TGFB1 and TGFBR2 mRNA expression was increased in patients with EBV infection. Similarly, mRNA expression of protein kinase C (PRKCB), MAP3K7, PDLIM7, and other members of TGFB1 and NF-κB signaling...
Source: Acta Microbiologica et Immunologica Hungarica - November 22, 2018 Category: Microbiology Authors: Filatova EN, Sakharnov NA, Knyazev DI, Utkin OV Tags: Acta Microbiol Immunol Hung Source Type: research

Iron deficiency anemia-related gut microbiota dysbiosis in infants and young children: A pilot study.
Abstract Nutritional iron deficiency (ID) causes not only anemia but also malfunction of the entire human organism. Recently, a role of the gut microbiota has been hypothesized, but limited data are available especially in infants. Here, we performed a pilot study to explore the gut microbiota in 10 patients with iron deficiency anemia (IDA) and 10 healthy controls aged 6-34 months. Fresh stool samples were collected from diapers, and the fecal microbiota was profiled by next-generation sequencing of the V3-V4 hypervariable region of the 16S rRNA gene. Except for diet diversity, the breastfeeding status at the enr...
Source: Acta Microbiologica et Immunologica Hungarica - November 12, 2018 Category: Microbiology Authors: Muleviciene A, D'Amico F, Turroni S, Candela M, Jankauskiene A Tags: Acta Microbiol Immunol Hung Source Type: research

Emergence of blaVEB and blaGES among VIM-producing Pseudomonas aeruginosa clinical isolates in Alexandria, Egypt.
Abstract Thirty-three Pseudomonas aeruginosa isolates, resistant to one or more β-lactams, were included in this study. Identification of tested strains was confirmed using MALDI-TOF/MS. Phenotypic and genotypic β-lactamase patterns were investigated. Most of the isolates were resistant to carbapenems (32 out of 33) and to the extended-spectrum cephalosporins (ESC) (30 out of 33). Phenotypically, the production of extended-spectrum beta-lactamase (ESBL), metallo-β-lactamases (MBL), and carbapenemases was detected in 10, 23, and 9 isolates, respectively. However, AmpC hyperproduction was not phenotyp...
Source: Acta Microbiologica et Immunologica Hungarica - November 7, 2018 Category: Microbiology Authors: Gaballah A, Elbaradei A, Elsheredy A, Kader O Tags: Acta Microbiol Immunol Hung Source Type: research

High rate of colistin and fosfomycin resistance among carbapenemase-producing Enterobacteriaceae in Turkey.
This study showed that the resistance rates of colistin and fosfomycin are high. The MDR and XDR strains of CRE are spreading in our region and thus a monitoring system for CRE should be followed. Moreover, the applicability of antimicrobial stewardship programs should be increased in all inpatient and outpatient settings. PMID: 30403361 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - November 7, 2018 Category: Microbiology Authors: Süzük Yıldız S, Kaşkatepe B, Şimşek H, Sarıgüzel FM Tags: Acta Microbiol Immunol Hung Source Type: research

Carbapenem resistance in Acinetobacter baumannii is associated with enhanced survival on hospital fabrics.
This study was conducted to analyze the association between some factors that may favor the dissemination of A. baumannii clinical isolates. A total of 47 clinical strains of A. baumannii were evaluated to carbapenem, the ability to produce biofilm, the susceptibility to some antiseptics, and the survival time on cotton fabrics. Most of the isolates were resistant to carbapenem (72.3%), produced biofilm (83%), and survived more than 7 (51%) days on fabrics. A significant association between decreased susceptibility to antiseptics containing chlorhexidine or triclosan and carbapenem resistance and survival on fabrics could ...
Source: Acta Microbiologica et Immunologica Hungarica - November 7, 2018 Category: Microbiology Authors: Lopez-Gigosos RM, Mariscal A, Gutierrez-Bedmar M, Real M, Mariscal-López E Tags: Acta Microbiol Immunol Hung Source Type: research

First detection of efrAB, an ABC multidrug efflux pump in Enterococcus faecalis in Tehran, Iran.
This study was conducted on 80 isolates of E. faecalis isolated from outpatients with urinary tract infection during a period of 1 year from April 2014 to April 2015. The antibiotic susceptibility patterns of isolates were determined by the disk diffusion method and presence of efrA and efrB genes was detected by PCR and sequencing. Minimum inhibitory concentrations (MICs) to ciprofloxacin (CIP) were measured with and without carbonyl cyanide 3-chlorophenylhydrazone (CCCP) by broth microdilution. The highest resistance rate was observed to erythromycin (83.3%) and the prevalence of efrA and efrB genes in all E. faecal...
Source: Acta Microbiologica et Immunologica Hungarica - September 24, 2018 Category: Microbiology Authors: Shiadeh SMJ, Hashemi A, Fallah F, Lak P, Azimi L, Rashidan M Tags: Acta Microbiol Immunol Hung Source Type: research

Prevalence of enterotoxin-encoding genes among diverse Shigella strains isolated from patients with diarrhea, southwest Iran.
This study aimed to investigate the presence of two important Shigella enterotoxins (ShET-1 and ShET-2), encoded by the set and sen genes, respectively, by polymerase chain reaction (PCR) assay among Shigella species isolated from children affected by shigellosis in Ahvaz, southwest of Iran. In this cross-sectional study, from June 2016 to April 2017, altogether 117 Shigella isolates were collected from fecal specimens of children aged
Source: Acta Microbiologica et Immunologica Hungarica - September 11, 2018 Category: Microbiology Authors: Moosavian M, Seyed-Mohammadi S, Sheikh AF, Khoshnood S, Dezfuli AA, Saki M, Ghaderian G, Shahi F, Abdi M, Abbasi F Tags: Acta Microbiol Immunol Hung Source Type: research

The relationship between structure and in vitro antistaphylococcal effect of plant-derived stilbenes.
This study reports the in vitro growth-inhibitory potential of eight naturally occurring stilbenes against six standard strains and two clinical isolates of S. aureus, using a broth microdilution method, and expressing the results as minimum inhibitory concentrations (MICs). Pterostilbene (MICs = 32-128 μg/ml), piceatannol (MICs = 64-256 μg/ml), and pinostilbene (MICs = 128 μg/ml) are among the active compounds that possess the strongest activity against all microorganisms tested, followed by 3'-hydroxypterostilbene, isorhapontigenin, oxyresveratrol, and rha...
Source: Acta Microbiologica et Immunologica Hungarica - September 11, 2018 Category: Microbiology Authors: Zakova T, Rondevaldova J, Bernardos A, Landa P, Kokoska L Tags: Acta Microbiol Immunol Hung Source Type: research

Genotypic analysis of S segment of Crimean-Congo hemorrhagic fever virus in Turkey.
In this study, serum samples (n = 26) were selected from CCHFV RNA-positive patients and subjected to sequence analysis of the gene regions encoding the S segments. According to the neighbor-joining analysis, the obtained partial sequences were linked to the European strain. The strains were closely related to Turkey-Kelkit06, Turkey 200310849 viruses, and viruses from Russia and Kosovo. The comparison with previously analyzed isolates from the GenBank showed 95%-99% sequence similarity. The isolates in phylogenetic branches were divided into two groups. AST, platelet, and APTT levels were found significantly...
Source: Acta Microbiologica et Immunologica Hungarica - September 11, 2018 Category: Microbiology Authors: Say Coskun US, Asık Z Tags: Acta Microbiol Immunol Hung Source Type: research

Prevalence of VIM- and GIM-producing Acinetobacter baumannii from patients with severe urinary tract infection.
Abstract Carbapenems are administered as the final drug of choice for treating complicated nosocomial infections caused by multidrug-resistant Acinetobacter baumannii strains. It is currently a worldwide issue that metallo-β-lactamases (MBLs) as carbapenem-hydrolyzing enzymes are one of the major drug resistance mechanisms. This investigation is thus aimed to assess the prevalence and characterize the MBL-producing strains of A. baumannii both by phenotypic assays and by genotypic characterization. A total of 73 isolates of A. baumannii were phenotypically and genotypically characterized from patients (N&thin...
Source: Acta Microbiologica et Immunologica Hungarica - August 16, 2018 Category: Microbiology Authors: Girija SA, Jayaseelan VP, Arumugam P Tags: Acta Microbiol Immunol Hung Source Type: research

High rate of carbapenem-resistant Klebsiella pneumoniae detected from hospital equipments in Iran.
This study showed that the environment of our hospitals is contaminated with CRKP and it emphasizes the importance of using standard methods for infection control. PMID: 30111161 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - August 16, 2018 Category: Microbiology Authors: Moghadampour M, Salari-Jazi A, Faghri J Tags: Acta Microbiol Immunol Hung Source Type: research

Transmissible silver resistance readily evolves in high-risk clone isolates of Klebsiella pneumoniae.
In this study, we investigated the capacity of both high-risk (CG14/15 and CG258) and minor clone strains of K. pneumoniae to develop resistance to silver. Resistance was induced in vitro in silver-susceptible but otherwise multidrug-resistant clinical isolates. Genetic alterations in the silver-resistant derivative strains with regard to the silver-susceptible isolates were investigated by whole-genome sequencing. The transferability of high-level resistance to silver was also tested. We demonstrated that the high-level resistance to silver can quickly evolve as a consequence of a single-point mutation either in the cusS ...
Source: Acta Microbiologica et Immunologica Hungarica - July 25, 2018 Category: Microbiology Authors: Hanczvikkel A, Füzi M, Ungvári E, Tóth Á Tags: Acta Microbiol Immunol Hung Source Type: research

Mycobacteria produce proteins involved in biofilm formation and growth-affecting processes.
Abstract The aim of this study was to determine the effect of mycobacterial proteins on mycobacterial biofilm formation and growth processes. We separated growth-affecting proteins (GEPs) from wild type of Mycobacterium bovis and ATCC strain of Mycobacterium avium subsp. avium. Our results showed that these mycobacteria-secreted GEPs are involved in biofilm formation, growth stimulatory, and inhibitory processes. Our findings suggest that GEP stimulated M. avium subsp. avium growth in vitro. Stimulation process was observed in mycobacteria affected with GEP extracted from M. avium subsp. avium. We found that both ...
Source: Acta Microbiologica et Immunologica Hungarica - July 19, 2018 Category: Microbiology Authors: Korabliovienė J, Mauricas M, Ambrozevičienė Č, Valius M, Kaupinis A, Čaplinskas S, Korabliov P Tags: Acta Microbiol Immunol Hung Source Type: research

The emergence of blaOXA-48 and blaNDM among ESBL-producing Klebsiella pneumoniae in clinical isolates of a tertiary hospital in Iran.
This study showed that the production of OXA-48 is one of the main mechanisms of resistance to carbapenems in CRKP isolates in Isfahan. In addition, the dissemination of NDM-producing CRKP isolates is a potential risk for the health care system of this area in the near future. PMID: 30024268 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - July 19, 2018 Category: Microbiology Authors: Moghadampour M, Rezaei A, Faghri J Tags: Acta Microbiol Immunol Hung Source Type: research

Fecal Helicobacter pylori glmM and 16S rRNA genes correlate with serum TNF- α and IL-1β cytokine fluctuations.
In this study, DNA was first extracted from the stool samples of infected individuals and used as DNA template to investigate the presence of glmM and 16S rRNA genes in PCR. The ELISA assay was employed to examine serum levels of TNF-α and IL-1β cytokines. According to statistical analysis, there was a significant correlation between the presence of glmM and 16S rRNA genes in the stool samples of infected persons and the serum oscillations of TNF-α and IL-1β cytokines. At the end of study and analysis of the data in case group with HPSAg+, 47.6% of the glmM gene and 23.6% of the 16S rRNA gene were pos...
Source: Acta Microbiologica et Immunologica Hungarica - July 19, 2018 Category: Microbiology Authors: Moradipour A, Khosravi A, Piri F Tags: Acta Microbiol Immunol Hung Source Type: research

Hormesis and immunity: A review.
Abstract The hormesis concept demonstrates that in contrast to the toxic effect of high doses of materials, irradiation, etc., low doses of them are beneficial and, in addition, help to eliminate (prevent) the deleterious effect of high doses given after it. By this effect, it is an important factor of (human) evolution protecting man from harmful impacts, similarly to the role of immunity. However, immunity is also continuously influenced by hormetic effects of environmental [chemical (pollutions), physical (background irradiations and heat), etc.] and medical (drugs and therapeutic irradiations) and food interac...
Source: Acta Microbiologica et Immunologica Hungarica - July 17, 2018 Category: Microbiology Authors: Csaba G Tags: Acta Microbiol Immunol Hung Source Type: research

Asymptomatic carriage of antibiotic-resistant Gram-positive cocci among different background populations in East Timor, Southeast Asia.
This study represents the first step toward the full characterization of potential pathogenic Gram-positive cocci present in the populations from East Timor. PMID: 30010391 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - July 16, 2018 Category: Microbiology Authors: Santos R, Grilo M, Araújo M, Monteiro JL, Oliveira M Tags: Acta Microbiol Immunol Hung Source Type: research

Comparison of polymerase chain reaction-restriction enzyme analysis method and DNA sequence analysis results in the identification of non-tuberculous mycobacteria.
zkütük AA Abstract The typing of non-tuberculous mycobacteria (NTM) is important from a clinical and epidemiological perspective. The polymerase chain reaction-restriction enzyme analysis (PRA) method and DNA sequence analysis method were utilized to target a gene region that codes the 65-kDa heat-shock protein for typing 150 suspected NTM samples isolated from the respiratory tract. Mycobacterium abscessus, Mycobacterium xenopi, Mycobacterium fortuitum, and Mycobacterium peregrinum were most frequently found by both methods. Six isolates that could not be defined by the PRA method were defined as Nocard...
Source: Acta Microbiologica et Immunologica Hungarica - July 16, 2018 Category: Microbiology Authors: Appak Ö, Türkel S, Esen N, Özkütük AA Tags: Acta Microbiol Immunol Hung Source Type: research

Characterization of macrolide-resistant non-invasive pneumococci in the pre-vaccine era in Serbia.
In conclusion, co-resistance to macrolides and penicillin in our non-invasive pneumococcal isolates is high. The majority of tested strains (∼80%) belonged to the four serotypes (19F, 14, 6B, and 23F) that are included in all conjugate vaccine formulations. PMID: 30010393 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - July 16, 2018 Category: Microbiology Authors: Popovic S, Hadnadjev M, Gajic I, Mijac V, Kekic D, Smitran A, Ranin L, Opavski N Tags: Acta Microbiol Immunol Hung Source Type: research

DNA microarray technology and bioinformatic web services.
Abstract The pan-genomic microarray technique is used for environmental and/or clinical studies. Although microarray is an accurate and sharp diagnostic tool, the expertized bioinformaticians were able to minimize the outcome biases and maximize the flexibility and accuracy of the technique. The knowledge of bioinformatics plays a key role in association with probe designing and the utilization of correct probe sets and platforms. This technique is divided into two parts as dry lab (in silico studies) and wet lab (in vitro studies). Each part covers the other and are known as complementary divisions. In the case o...
Source: Acta Microbiologica et Immunologica Hungarica - July 16, 2018 Category: Microbiology Authors: Behzadi P, Ranjbar R Tags: Acta Microbiol Immunol Hung Source Type: research

Immunity and longevity.
Abstract The role of immune system is to protect the organism from the not built-in program-like alterations inside and against the agents penetrating from outside (bacteria, viruses, and protozoa). These functions were developed and formed during the evolution. Considering these functions, the immune system promotes the lengthening of lifespan and helps longevity. However, some immune functions have been conveyed by men to medical tools (e.g., pharmaceuticals, antibiotics, and prevention), especially in our modern age, which help the struggle against microbes, but evolutionarily weaken the immune system. Agi...
Source: Acta Microbiologica et Immunologica Hungarica - July 3, 2018 Category: Microbiology Authors: Csaba G Tags: Acta Microbiol Immunol Hung Source Type: research

Influence of subinhibitory antibiotic concentration on Streptococcus pyogenes adherence and biofilm production.
In this study, the focus was on the effects of sub-MICs of the antibiotics on adherence, hydrophobicity, and biofilm formation by two groups of Streptococcus pyogenes strains, which were responsible for different clinical cases. The aim of this study was to explore the effects of sub-MICs of penicillin, ceftriaxone, erythromycin, and clindamycin on adherence, surface hydrophobicity, and biofilm biomass in two selected collections of group A streptococcus (GAS): strains isolated from carriers (CA) and strains isolated from patients with tonsillopharyngitis (TPh). Isolates were tested for hydrophobicity to xylene, adherence,...
Source: Acta Microbiologica et Immunologica Hungarica - April 24, 2018 Category: Microbiology Authors: Šmitran A, Vuković D, Opavski N, Gajić I, Marinković J, Božić L, Živanović I, Kekić D, Popović S, Ranin L Tags: Acta Microbiol Immunol Hung Source Type: research

Analysis of antigen conservation and inactivation of gamma-irradiated avian influenza virus subtype H9N2.
Abstract Avian influenza (AI) A subtype H9N2 virus belongs to Orthomyxoviridae family and causes low-pathogenic disease AI. The use of gamma-irradiated viral antigens has been developed in the production of effective vaccines. In this research, LPAIV H9N2 strain, A/Chicken/IRN/Ghazvin/2001, was multiplied on SPF eggs and irradiated by a Nordian gamma cell instrument. Irradiated and non-irradiated AI virus (AIV) samples were titrated by EID50 method and hemagglutinin (HA) antigen was analyzed by HA test as the WHO pattern method. Infectivity of irradiated virus was determined by egg inoculation method during four b...
Source: Acta Microbiologica et Immunologica Hungarica - April 24, 2018 Category: Microbiology Authors: Salehi B, Motamedi-Sedeh F, Madadgar O, Khalili I, Ghalyan Chi Langroudi A, Unger H, Wijewardana V Tags: Acta Microbiol Immunol Hung Source Type: research

Effect of amino acid substitution in New Delhi metallo- β-lactamase on carbapenem susceptibility.
Effect of amino acid substitution in New Delhi metallo-β-lactamase on carbapenem susceptibility. Acta Microbiol Immunol Hung. 2018 Apr 13;:1-9 Authors: Düzgün AÖ Abstract The aim of this work was investigation of clinically important amino acid substitutions of NDM-1 variants. A blaNDM-1 gene was cloned into expression vector pET100/D-TOPO. The sequence of NDM-1 variants with substituted amino acids was determined by ClustalW program. A pET100/D-TOPO + blaNDM-1 was used to generate the alanine mutations at different positions, such as NDM-2 (P28A), NDM-3 (D95A), NDM-4...
Source: Acta Microbiologica et Immunologica Hungarica - April 13, 2018 Category: Microbiology Authors: Düzgün AÖ Tags: Acta Microbiol Immunol Hung Source Type: research

Risk factors for Clostridium difficile infections in Baranya County, Southern Hungary.
aacute;cs B, Péterfi Z Abstract In the past decade, Clostridium difficile infections (CDIs) have become a major public health challenge. Their epidemiology has radically changed with a significant rise in the number of cases and an increase in severe episodes. Recurrence and failure of conventional treatments are very common. Furthermore, a spread of CDI has emerged in general population without the usual risk factors (unexposed to antibiotic treatment, young people, etc.). The conventional treatments (metronidazole and vancomycin) are still effective and are the first-line antibiotics with new recommendati...
Source: Acta Microbiologica et Immunologica Hungarica - April 13, 2018 Category: Microbiology Authors: Vigvári S, Sipos D, Kappéter Á, Feiszt Z, Kovács B, Péterfi Z Tags: Acta Microbiol Immunol Hung Source Type: research