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High prevalence of Staphylococcus aureus nasal carriage among children in Szolnok, Hungary.
acute;th A, Kardos S, Dobay O Abstract We collected nasal samples from 1,390 healthy 3-7 years old children in Szolnok city, Hungary, in 2012. We detected 476 Staphylococcus aureus isolates from 474 children. In two occasions, two different S. aureus were isolated, based on hemolysis type and pulsed-field gel electrophoresis pattern. S. aureus carriage rate was calculated to be 34.1% similar to others studies. Male gender was found to be a risk factor for carriage by statistical analysis. Altogether, four methicillin-resistant S. aureus (MRSA) strains were detected by mecA polymerase chain reaction, which means 0....
Source: Acta Microbiologica et Immunologica Hungarica - December 18, 2017 Category: Microbiology Authors: Laub K, Tóthpál A, Kovács E, Sahin-Tóth J, Horváth A, Kardos S, Dobay O Tags: Acta Microbiol Immunol Hung Source Type: research

Quorum-sensing network-associated gene regulation in Gram-positive bacteria.
Abstract Quorum sensing (QS) is the chemical communication processes between bacteria, which may be inter-genus or intra-genus. In general, several physiological functions, such as nutrient uptake, competence development, biofilm formation, sporulation, and toxin secretion, are accomplished through QS process. The QS (cell density-dependent process) circuit in Gram-positive bacteria consists mainly of two parts: an inducer molecule and a receptor protein. The binding of inducer molecule to receptor activates the target gene, which then performs the necessary function in bacteria. In the past few years, several inv...
Source: Acta Microbiologica et Immunologica Hungarica - December 15, 2017 Category: Microbiology Authors: Banerjee G, Ray AK Tags: Acta Microbiol Immunol Hung Source Type: research

Does subinhibitory concentrations of clinically important antibiotic induce biofilm production of Enterococcus faecium strains?
Abstract Biofilm structures are the most resistant form of active microorganisms against sanitation, disinfection, and sterilization processes. One of the specific properties of biofilm is the development of antibiotic resistance that can be up to 1,000-fold greater than planktonic cells. Enterococcus faecium is a human pathogen that causes nosocomial bacteremia and at the present time, it is well known that most of the chronic infections are biofilm-based. Recent evidence suggested that subinhibitory concentrations (sub-MICs) of antibiotics have an important role in the evolution of antibiotic resistance and indu...
Source: Acta Microbiologica et Immunologica Hungarica - December 15, 2017 Category: Microbiology Authors: Yuksel FN, Karatug NT, Akcelik M Tags: Acta Microbiol Immunol Hung Source Type: research

The recombinant fusion protein CFP10-ESAT6-dIFN has protective effect against tuberculosis in guinea pigs.
SE, Deineko EV Abstract Development of effective vaccine candidates against tuberculosis (TB) is currently the most important challenge in the prevention of this disease since the BCG vaccine fails to guarantee a lifelong protection, while any other approved vaccine with better efficiency is still absent. The protective effect of the recombinant fusion protein CFP10-ESAT6-dIFN produced in a prokaryotic expression system (Escherichia coli) has been assessed in a guinea pig model of acute TB. The tested antigen comprises the Mycobacterium tuberculosis (Mtb) proteins ESAT6 and CFP10 as well as modified human γ-...
Source: Acta Microbiologica et Immunologica Hungarica - December 15, 2017 Category: Microbiology Authors: Permyakova NV, Belavin PA, Pirozhkova DS, Ufimtseva EG, Rozov SM, Mursalimov SR, Sidorchuk YV, Uvarova EA, Zagorskaya AA, Marenkova TV, Bannikova SV, Demidov EA, Starostin KV, Kravchenko MA, Vakhrusheva DV, Berdnikov RB, Eremeeva NI, Skornyakov SN, Peltek Tags: Acta Microbiol Immunol Hung Source Type: research

Faecal microbiota transplantation for Clostridium difficile infection using a lyophilized inoculum from non-related donors: A case series involving 19 patients.
In this study, our objective was to evaluate the rate of resolution of diarrhea following administration of lyophilized and resolved FMT via a nasogastric (NG) tube. We recruited 19 patients suffered from laboratory-confirmed C. difficile infection. Each of them was treated by lyophilized and resolved inoculum through a NG tube. One participant succumbed following the procedure due to unrelated diseases. Out of 18 cases, 15 patients reportedly experienced a resolution of the symptoms. One patient was treated with another course of antibiotics, and two of the non-responders were successfully retreated with another course of...
Source: Acta Microbiologica et Immunologica Hungarica - December 14, 2017 Category: Microbiology Authors: Vigvári S, Sipos D, Solt J, Vincze Á, Kocsis B, Nemes Z, Kappéter Á, Feiszt Z, Kovács B, Péterfi Z Tags: Acta Microbiol Immunol Hung Source Type: research

Sequence analysis of Schmallenberg virus genomes detected in Hungary.
In this study, we determined the complete coding sequence of seven Hungarian SBV genomes to expand our knowledge about the genetic diversity of circulating field strains. The samples originated from the first case, an aborted cattle fetus without malformation collected in 2012, and from the blood samples of six adult cattle in 2014. The Hungarian SBV sequences shared ≥99.3% nucleotide (nt) and ≥97.8% amino acid (aa) identity with each other, and ≥98.9 nt and ≥96.7% aa identity with reference strains. Although phylogenetic analyses showed low resolution in general, the M sequences of cattle and sheep origin SBV ...
Source: Acta Microbiologica et Immunologica Hungarica - December 14, 2017 Category: Microbiology Authors: Fehér E, Marton S, Tóth ÁG, Ursu K, Wernike K, Beer M, Dán Á, Bányai K Tags: Acta Microbiol Immunol Hung Source Type: research

Characterization of the Rhodococcus sp. MK1 strain and its pilot application for bioremediation of diesel oil-contaminated soil.
e;cs T, Rákhely G, Perei K Abstract Petroleum hydrocarbons and derivatives are widespread contaminants in both aquifers and soil, their elimination is in the primary focus of environmental studies. Microorganisms are key components in biological removal of pollutants. Strains capable to utilize hydrocarbons usually appear at the contaminated sites, but their metabolic activities are often restricted by the lack of nutrients and/or they can only utilize one or two components of a mixture. We isolated a novel Rhodococcus sp. MK1 strain capable to degrade the components of diesel oil simultaneously. The draft ...
Source: Acta Microbiologica et Immunologica Hungarica - December 6, 2017 Category: Microbiology Authors: Kis ÁE, Laczi K, Zsíros S, Kós P, Tengölics R, Bounedjoum N, Kovács T, Rákhely G, Perei K Tags: Acta Microbiol Immunol Hung Source Type: research

Aflatoxin B1 detoxification by cell-free  extracts of Rhodococcus strains.
Aflatoxin B1 detoxification by cell-free extracts of Rhodococcus strains. Acta Microbiol Immunol Hung. 2017 Dec 01;:1-16 Authors: Risa A, Divinyi DM, Baka E, Krifaton C Abstract Aflatoxin B1 (AFB1) produced by Aspergillus molds is a genotoxic and carcinogenic mycotoxin. For the elimination of mycotoxins from food and feed, biodetoxification can be a successful tool. The aim of this study was to reveal biodetoxification with the cell-free extracts of Rhodococcus erythropolis NI1 and Rhodococcus rhodochrous NI2, which have been already proved to detoxify AFB1. Extracellular matrices of cultures and...
Source: Acta Microbiologica et Immunologica Hungarica - December 1, 2017 Category: Microbiology Authors: Risa A, Divinyi DM, Baka E, Krifaton C Tags: Acta Microbiol Immunol Hung Source Type: research

Emergence of fosfomycin resistance among isolates of Escherichia coli harboring extended-spectrum and AmpC β-lactamases.
This study reports the first detection of fosfomycin resistance in Iran. This study indicated the increasing prevalence of UTI isolates of E. coli-harboring ESBL and AmpC β-lactamases genes in Iran. Therefore, due to the high rate of blaDHA and blaCMY genes and emergence of fosfomycin-resistant E. coli isolates, we recommend continuous monitoring of antibiotic resistance as well as attention to guidelines of infection controls. PMID: 29137487 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - November 15, 2017 Category: Microbiology Authors: Bahramian A, Eslami G, Hashemi A, Tabibi A, Heidary M Tags: Acta Microbiol Immunol Hung Source Type: research

Abstracts of the 26th Semmelweis Symposium 2017 - New Challenges in Microbiology, 9-10 November, 2017 Budapest, Hungary.
Authors: PMID: 29106799 [PubMed] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - November 1, 2017 Category: Microbiology Tags: Acta Microbiol Immunol Hung Source Type: research

Abstracts of the Annual Meeting of the Hungarian Society for Microbiology, October 19-21, 2016, Keszthely, Hungary.
Authors: PMID: 29106802 [PubMed] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - November 1, 2017 Category: Microbiology Tags: Acta Microbiol Immunol Hung Source Type: research

Abstracts Of the 5th Central European Forum for Microbiology, October 18-20, 2017, Keszthely, Hungary.
Authors: PMID: 29106803 [PubMed] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - November 1, 2017 Category: Microbiology Tags: Acta Microbiol Immunol Hung Source Type: research

Occurrence of vancomycin-resistant Staphylococcus aureus in the oral cavity of patients with dental caries.
Abstract Oral streptococci are the major group of microbes isolated from oral microflora. They represent frequent pathogens of infective endocarditis (IE), and it is assumed that in most of the cases oral streptococci are acquired via mucosa layer of oral cavity. Staphylococcus aureus is also frequently isolated from IE as it accounts for 20%-30% of all cases. Vancomycin has been the most reliable therapeutic agent against infections caused by methicillin-resistant S. aureus (MRSA). The main objective of this study was to examine the occurrence of S. aureus species in dental caries specimens. Antimicrobial suscept...
Source: Acta Microbiologica et Immunologica Hungarica - September 11, 2017 Category: Microbiology Authors: Vellappally S, Divakar DD, Al Kheraif AA, Ramakrishnaiah R, Alqahtani A, Dalati MHN, Anil S, Khan AA, Harikrishna Varma PR Tags: Acta Microbiol Immunol Hung Source Type: research

Resurgence of yellow fever in Brazil: Overview and possible control options.
PMID: 28889757 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - September 11, 2017 Category: Microbiology Authors: Khan AW, Nasim Z, Zahir F, Khan MA, Ali A, Ali S, Khan AH Tags: Acta Microbiol Immunol Hung Source Type: research

MALDI-TOF MS versus 16S rrna sequencing: Minor discrepancy between tools in identification of Bacteroides isolates.
te;czy K, Melegh SZ, Urbán E Abstract Members of the genus Bacteroides are important components of the normal microbiota of gastrointestinal tract; however, as opportunistic pathogens are also associated with severe or even life-threatening infections with significant mortality. Various species within Bacteroides fragilis group are phenotypically very similar; thus, their identifications with traditional-automated biochemical methods are frequently inaccurate. The identification of the newly discovered or reclassified bacteria can be doubtful because of the lack of biochemical profile in the database of the...
Source: Acta Microbiologica et Immunologica Hungarica - September 11, 2017 Category: Microbiology Authors: Sárvári KP, Sóki J, Iván M, Miszti C, Latkóczy K, Melegh SZ, Urbán E Tags: Acta Microbiol Immunol Hung Source Type: research

Efficiency of temporary storage of geothermal waters in a lake system: Monitoring the changes of water quality and bacterial community structures.
In this study, the physical and chemical properties and changes in the bacterial community structure of a reservoir lake system in southeast region of Hungary were monitored and compared through 2 years, respectively. The values of biological oxygen demand, concentrations of ammonium ion, total inorganic nitrogen, total phosphorous, and total phenol decreased, whereas oxygen saturation, total organic nitrogen, pH, and conductivity increased during the storage period. Bacterial community structure of water and sediment samples was compared by denaturing gradient gel electrophoresis (DGGE) following the amplification of the ...
Source: Acta Microbiologica et Immunologica Hungarica - September 5, 2017 Category: Microbiology Authors: Szirányi B, Krett G, Kosáros T, Janurik E, Pekár F, Márialigeti K, Borsodi AK Tags: Acta Microbiol Immunol Hung Source Type: research

High prevalence of OXA-type carbapenemases among Acinetobacter baumannii strains in a teaching hospital of Tehran.
In this study, 100 isolates of CRAB from hospitalized patients during 2015-2016 at Imam Khomeini Hospital were investigated to determine the rates of multidrug-resistant (MDR) and extensively drug-resistant (XDR) strains using Kirby-Bauer disk diffusion method. The minimum inhibitory concentrations (MICs) of six antibiotics were determined by broth microdilution method. Multiplex polymerase chain reaction (PCR) was performed to detect blaOXA-51 like and blaOXA-58 like, blaOXA-23 like, and blaOXA-24 like that are encoding resistance to carbapenems. All CRAB isolates were MDR and XDR and 2% of the...
Source: Acta Microbiologica et Immunologica Hungarica - September 5, 2017 Category: Microbiology Authors: Zafari M, Feizabadi MM, Jafari S, Abdollahi A, Sabokbar A Tags: Acta Microbiol Immunol Hung Source Type: research

Emergence of armA and rmtB genes among VIM, NDM, and IMP metallo- β-lactamase-producing multidrug-resistant Gram-negative pathogens.
This study was aimed to establish the pattern of antibiotic sensitivity and relevance of antimicrobial resistance in aerobic Gram-negative bacilli. A total of 103 aerobic Gram-negative bacteria namely Escherichia coli, Klebsiella pneumoniae, Enterobacter spp., Citrobacter koserii, Proteus spp., and Pseudomonas aeruginosa were collected from tertiary care centers around Chennai. Kirby-Bauer Disk Diffusion test and study for genes of cephalosporin, carbapenem, and aminoglycoside resistance were done. A descriptive analysis of the data on altogether 103 clinical urine isolates was performed. All strains showed susceptibility ...
Source: Acta Microbiologica et Immunologica Hungarica - September 5, 2017 Category: Microbiology Authors: Gopalakrishnan S, Kamalanathan A, Rajan S, Bhagat VM, Ali MKS Tags: Acta Microbiol Immunol Hung Source Type: research

First report of coexistence of AmpC beta-lactamase genes in Klebsiella pneumoniae strains isolated from burn patients.
In conclusion, K. pneumoniae is becoming a serious problem in burn patients. Accurate and precise methods and guidelines should be designed for detection of antibiotic-resistant mechanisms. Our data showed the high rate of AmpC beta-lactamase among K. pneumoniae isolated from burn patients, which limit the treatment options. Therefore, the results of this study can provide evidence to help for appropriate treatment of burn patients. PMID: 28859497 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2017 Category: Microbiology Authors: Ghanavati R, Darban-Sarokhalil D, Navab-Moghadam F, Kazemian H, Irajian G, Razavi S Tags: Acta Microbiol Immunol Hung Source Type: research

Influence of human milk oligosaccharides on adherence of bifidobacteria and clostridia to cell lines.
Abstract Adhesion of gut bacteria to the intestinal epithelium is the first step in their colonization of the neonatal immature gut. Bacterial colonization of the infant gut is influenced by several factors, of which the most important are the mode of delivery and breast-feeding. Breast-fed infants ingest several grams of human milk oligosaccharides (HMOs) per day, which can become receptor decoys for intestinal bacteria. The most abundant intestinal bacteria in vaginally delivered infants are bifidobacteria, whereas infants born by cesarean section are colonized by clostridia. The influence of HMOs on the adhesio...
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2017 Category: Microbiology Authors: Musilova S, Modrackova N, Doskocil I, Svejstil R, Rada V Tags: Acta Microbiol Immunol Hung Source Type: research

Prevalence and molecular typing of methicillin-resistant Staphylococcus aureus carrying Panton-Valentine leukocidin gene.
Abstract Panton-Valentine leukocidin (pvl) toxin is an important virulence factor of Staphylococcus aureus. The main genes are coa and spa for distinguishing and typing of S. aureus isolates. The aim of this study was to investigate antibiotic resistance, presence of mecA and pvl genes, as well as epidemiological typing of these isolates according to polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method in clinical sample isolated from Rasht city, Iran. A total of 250 clinical samples have been isolated from different hospitals. First, isolates of S. aureus were identified through mi...
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2017 Category: Microbiology Authors: Hesari MR, Salehzadeh A, Darsanaki RK Tags: Acta Microbiol Immunol Hung Source Type: research

Emergence of NDM-1 among carbapenem-resistant Klebsiella pneumoniae in Iraqi hospitals.
This study reports NDM-1 for the first time among Klebsiella pneumoniae from hospitalized patients in Baghdad, Iraq. Fifty-five clinical isolates of K. pneumoniae resistant to carbapenem were investigated from burned wounds, sputum, and blood samples. The susceptibility to different antibiotics was tested by VITEK-2 system. All strains were multidrug-resistant and they showed nine different antimicrobial-resistant patterns (A-I) and the most effective antibiotic on these strains was levofloxacin (85.45%). The phenotypic detection of carbapenemases by MASTDISCS D70C revealed 29 (52.73%) strains were MBL-producing, out of 55...
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2017 Category: Microbiology Authors: Hussein NH Tags: Acta Microbiol Immunol Hung Source Type: research

Is there a hormonal regulation of phagocytosis at unicellular and multicellular levels? A critical review.
Abstract Phagocytosis is an ancient cell function, which is similar at unicellular and multicellular levels. Unicells synthesize, store, and secrete multicellular (mammalian) hormones, which influence their phagocytosis. Amino acid hormones, such as histamine, serotonin, epinephrine, and melatonin stimulate phagocytosis, whereas peptide hormones, such as adrenocorticotropic hormone (ACTH), insulin, opioids, arginine vasopressin, and atrial natriuretic peptide decreased it, independently on their chemical structure or function in multicellulars. Macrophage phagocytosis of multicellulars is also stimulated by amino ...
Source: Acta Microbiologica et Immunologica Hungarica - September 1, 2017 Category: Microbiology Authors: Csaba G Tags: Acta Microbiol Immunol Hung Source Type: research

High frequency of methicillin-resistant Staphylococcus aureus (MRSA) with SCCmec type III and spa type t030 in Karaj's teaching hospitals, Iran.
In conclusion, high frequency of spa t030 with SCCmec type III and MRSA phenotype illustrated circulating of one of the antibiotic-resistant strains in ICU of Karaj's teaching hospitals and emphasizes the need for ongoing molecular surveillance, antibiotic susceptibility monitoring, and infection control. PMID: 28836817 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - August 24, 2017 Category: Microbiology Authors: Bayat B, Zade MH, Mansouri S, Kalantar E, Kabir K, Zahmatkesh E, Sepehr MN, Naseri MH, Darban-Sarokhalil D Tags: Acta Microbiol Immunol Hung Source Type: research

Colistin resistance associated with outer membrane protein change in  Klebsiella pneumoniae and Enterobacter asburiae.
This study demonstrated that OMP differences were between colistin-susceptible and -resistant counterpart strains. The altered Gram-negative cell wall may contribute to acquired colistin resistance in Enterobacteriaceae. PMID: 28629229 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - June 20, 2017 Category: Microbiology Authors: Kádár B, Kocsis B, Tóth Á, Kristóf K, Felső P, Kocsis B, Böddi K, Szabó D Tags: Acta Microbiol Immunol Hung Source Type: research

Association between Toxoplasma gondii types and outcomes of human infection: A meta-analysis.
Abstract The virulence and pathogenicity of various types of Toxoplasma gondii differ considerably in mice. Recent studies have claimed that similar phenomenon was observed in humans, but no relevant studies have been performed to validate this finding. In addition, reports showing association between a given T. gondii type and outcomes of human infection yielded conflicting results. To provide a more precise estimation of the association and a more reliable conclusion on this subject, we performed this meta-analysis. Relevant literatures were identified in multiple databases and selected based on strict screening...
Source: Acta Microbiologica et Immunologica Hungarica - June 20, 2017 Category: Microbiology Authors: Xia J, Cheng XY, Wang XJ, Peng HJ Tags: Acta Microbiol Immunol Hung Source Type: research

Immune responses to killed reassorted influenza virus supplemented with natural adjuvants.
In this study, we investigated the immunomodulatory effects of a supplemented killed influenza virus (V) by Echinacea purpurea (E) and Nigella sativa (N) extracts and effect of changing the route of immunization from intramuscular (IM) to intraperitoneal (IP). At the 2(nd)-, 3(rd)- and 4(th)-week post-IM immunizations (WPIMI), the supplemented V with N (VN) induced the most significant IgM response unlike N alone. At the 2(nd) WPIMI, V or VN induced the highest significant IgG levels. At the 2(nd)-week post-IP immunization (WPIPI), E and VN induced the most significant IgG levels. Both at the 3(rd) and 4(th) WPIMI or WPIPI...
Source: Acta Microbiologica et Immunologica Hungarica - June 19, 2017 Category: Microbiology Authors: Razin MAF, Osman A, Ali MA, Bahgat MM, Maghraby AS Tags: Acta Microbiol Immunol Hung Source Type: research

Complex multicellular functions at a unicellular eukaryote level: Learning, memory, and immunity.
Abstract According to experimental data, eukaryote unicellulars are able to learn, have immunity and memory. Learning is carried out in a very primitive form, and the memory is not neural but an epigenetic one. However, this epigenetic memory, which is well justified by the presence and manifestation of hormonal imprinting, is strong and permanent in the life of cell and also in its progenies. This memory is epigenetically executed by the alteration and fixation of methylation pattern of genes without changes in base sequences. The immunity of unicellulars is based on self/non-self discrimination, which leads to t...
Source: Acta Microbiologica et Immunologica Hungarica - June 19, 2017 Category: Microbiology Authors: Csaba G Tags: Acta Microbiol Immunol Hung Source Type: research

Protection against shigellosis caused by Shigella dysenteriae serotype 4 in guinea pigs using Escherichia albertii DM104 as a live vaccine candidate strain.
In this study, we assessed three different immunization routes, such as intranasal, oral, and intrarectal routes, and revealed differences in immune responses by measuring both the serum IgG and mucosal IgA antibody titers. Protective efficacy of different routes of immunization was also determined by challenging immunized guinea pigs against live S. dysenteriae. It was found that intranasal immunization showed promising results in terms of antibody response and protective efficacy. All these results reconfirm our previous findings and additionally point out that the intranasal immunization of the environmental E. albertii...
Source: Acta Microbiologica et Immunologica Hungarica - June 9, 2017 Category: Microbiology Authors: Chowdhury FM, Rahman MZ, Sarkar MMH, Rabbi F, Khan SI, Ahsan CR, Birkeland NK Tags: Acta Microbiol Immunol Hung Source Type: research

A gain-of-function mutation of STAT1: A novel genetic factor contributing to chronic mucocutaneous candidiasis.
Abstract Heterozygous gain-of-function (GOF) mutations in the signal transducer and activator of transcription 1 (STAT1) have increasingly been identified as a genetic cause of autosomal-dominant (AD) chronic mucocutaneous candidiasis (CMC). In this article, we describe a 33-year-old man who experienced chronic refractory candidiasis, recurrent otitis media, and pneumonia resulting in bronchiectasis, severe oral and esophageal candidiases with strictures associated with hypothyroidism and immune hemolytic anemia. His son also suffered from persistent candidiasis, chronic diarrhea, poor weight gain, and pneumonia t...
Source: Acta Microbiologica et Immunologica Hungarica - June 9, 2017 Category: Microbiology Authors: Eslami N, Tavakol M, Mesdaghi M, Gharegozlou M, Casanova JL, Puel A, Okada S, Arshi S, Bemanian MH, Fallahpour M, Molatefi R, Seif F, Zoghi S, Rezaei N, Nabavi M Tags: Acta Microbiol Immunol Hung Source Type: research

A deletion mutation in nfxB of in vitro-induced moxifloxacin-resistant Pseudomonas aeruginosa confers multidrug resistance.
In this study, MDR mechanism in Pseudomonas aeruginosa induced via moxifloxacin (MOX) pressure was investigated. After serial MOX [concentration of 0.5 × the minimum inhibitory concentration (MIC)] exposure, the fully susceptible P. aeruginosa ATCC 9027 strain has increased its MIC not only toward MOX (1→128 mg/L) but also to other antibiotics. Furthermore, this MOX-exposed strain did not revert to antibiotic-sensitive phenotype when being cultured in antibiotic-free medium for 12 days. No mutation was observed for FQ-target (gyrA and parC) or most investigated efflux regulatory genes (mexT, m...
Source: Acta Microbiologica et Immunologica Hungarica - May 31, 2017 Category: Microbiology Authors: Thai VC, Pham HV, Nguyen DNM, Lambert P, Nguyen TTH Tags: Acta Microbiol Immunol Hung Source Type: research

High prevalence of NDM metallo- β-lactamase among ESBL-producing Escherichia coli İsolates.
This study aimed to determine the frequency of β-lactamase genes and antibiotic resistance rates of 140 extended-spectrum β-lactamase (ESBL)-producing Escherichia coli isolates obtained from urinary tract infection in Ordu Province, Turkey. Isolates were identified by classic methods and by automated system. ESBL production was confirmed by double disk synergy test and antimicrobial susceptibility was investigated by disk diffusion method. All isolates were screened for β-lactamase coding genes from three groups (A, B, and D) by polymerase chain reaction. The highest rate of susceptible isolates was observed...
Source: Acta Microbiologica et Immunologica Hungarica - April 19, 2017 Category: Microbiology Authors: Çetinkol Y, Sandalli C, Çalgin MK, Yildirim AA, Akyildiz E, Karaman E, Çiçek AÇ Tags: Acta Microbiol Immunol Hung Source Type: research

Advances in diagnosis and treatment of Helicobacter pylori infection.
Abstract Helicobacter pylori is a Gram-negative motile bacterium causative agent of acute and chronic digestive and extra-digestive human infections. According to different reports worldwide, H. pylori symptomatic and asymptomatic infections are a global problem. The statistical investigations show a percentage of 50 for people who are involved in H. pylori acute/chronic digestive and/or extra-digestive infections around the world. This review focuses on digestive and extra-digestive diseases caused by H. pylori, the related virulence factors, diagnostic techniques including non-invasive and invasive diagnostics a...
Source: Acta Microbiologica et Immunologica Hungarica - March 5, 2017 Category: Microbiology Authors: Ranjbar R, Behzadi P, Farshad S Tags: Acta Microbiol Immunol Hung Source Type: research

Effect of primycin on growth-arrested cultures and cell integrity of Staphylococcus aureus.
Abstract Bactericidal effect against non-dividing bacteria is a very advantageous, but rare characteristic among antimicrobial agents, mostly possessed by those affecting the cell membrane. These kinds of agents can kill bacterial cells without lysis. We assessed these characteristics on primycin, a topical anti-staphylococcal agent highly effective against prevalent multiresistant strains, as it also acts on the cell membrane. In time-kill studies, primycin preserved its bactericidal activity against growth-arrested Staphylococcus aureus cultures. The bactericidal action was slower against growth-arrested culture...
Source: Acta Microbiologica et Immunologica Hungarica - March 5, 2017 Category: Microbiology Authors: Feiszt P, Schneider G, Emődy L Tags: Acta Microbiol Immunol Hung Source Type: research

Study on the glutathione metabolism of the filamentous fungus Aspergillus nidulans.
;, Pócsi I Abstract Yeast protein sequence-based homology search for glutathione (GSH) metabolic enzymes and GSH transporters demonstrated that Aspergillus nidulans has a robust GSH uptake and metabolic system with several paralogous genes. In wet laboratory experiments, two key genes of GSH metabolism, gcsA, and glrA, encoding γ-l-glutamyl-l-cysteine synthetase and glutathione reductase, respectively, were deleted. The gene gcsA was essential, and the ΔgcsA mutant required GSH supplementation at considerably higher concentration than the Saccharomyces cerevisiae gsh1 mutant (8-10 mmol l(-1) vs....
Source: Acta Microbiologica et Immunologica Hungarica - March 5, 2017 Category: Microbiology Authors: Bakti F, Király A, Orosz E, Miskei M, Emri T, Leiter É, Pócsi I Tags: Acta Microbiol Immunol Hung Source Type: research

First description of a rifampicin-resistant Neisseria meningitidis serogroup Y strain causing recurrent invasive meningococcal disease in Hungary.
;szka Z, Bognár Z, Erdősi T Abstract A Hungarian soldier previously immunized against Neisseria meningitidis by quadrivalent polysaccharide vaccine was twice infected with meningococci within six weeks. The patient was treated with ceftriaxone during both episodes and he successfully recovered. His close contacts received rifampicin prophylaxis. An investigation was performed to characterize the genetic background of the pathogens to ascertain if the recurrent invasive meningococcal disease was caused by the same strain and to find out the reason for reinfection. Both meningococci belonged to the fine type...
Source: Acta Microbiologica et Immunologica Hungarica - February 20, 2017 Category: Microbiology Authors: Tóth Á, Berta B, Tirczka T, Jekkel C, Ábrahám A, Prohászka Z, Bognár Z, Erdősi T Tags: Acta Microbiol Immunol Hung Source Type: research

Epidemiology and antibiotic sensitivity of Staphylococcus aureus nasal carriage in children in Hungary.
S, Dobay O Abstract The aim of this study was to assess the Staphylococcus aureus nasal carriage rate in healthy children all over Hungary and to specify some risk factors, the antibiotic resistance patterns of the bacteria, and their genetic relatedness. In total, 878 children (aged 3-6 years) were screened at 21 day-care centers in 16 different cities in Hungary, between February 2009 and December 2011. Samples taken from both nostrils were cultured on blood agar, and suspected S. aureus isolates were identified by β-hemolysis, catalase positivity, clump test, and nucA PCR. Methicillin-resistant strains we...
Source: Acta Microbiologica et Immunologica Hungarica - February 20, 2017 Category: Microbiology Authors: Laub K, Tóthpál A, Kardos S, Dobay O Tags: Acta Microbiol Immunol Hung Source Type: research

Biofilm formation of Achromobacter xylosoxidans on contact lens.
Abstract Achromobacter spp. may contaminate lenses, lens cases, and contact lens solutions and cause ocular infections. The aim of this study was to investigate the possibility of isolated strain of Achromobacter xylosoxidans to form biofilm on the surface of soft contact lenses (CL), to quantify the production of the formed biofilm, and compare it with the reference strains (Pseudomonas aeruginosa, Staphylococcus aureus, and Haemophilus influenzae). Bacterial strain isolated from one contact lens case was identified as A. xylosoxidans using Vitek2 Automated System. Biofilm forming capacity of isolated stra...
Source: Acta Microbiologica et Immunologica Hungarica - February 19, 2017 Category: Microbiology Authors: Konstantinović N, Ćirković I, Đukić S, Marić V, Božić DD Tags: Acta Microbiol Immunol Hung Source Type: research

Virulotyping of Shigella spp. isolated from pediatric patients in Tehran, Iran.
Abstract Shigellosis is a considerable infectious disease with high morbidity and mortality among children worldwide. In this survey the prevalence of four important virulence genes including ial, ipaH, set1A, and set1B were investigated among Shigella strains and the related gene profiles identified in the present investigation, stool specimens were collected from children who were referred to two hospitals in Tehran, Iran. The samples were collected during 3 years (2008-2010) from children who were suspected to shigellosis. Shigella spp. were identified throughout microbiological and serological tests and then s...
Source: Acta Microbiologica et Immunologica Hungarica - February 19, 2017 Category: Microbiology Authors: Ranjbar R, Bolandian M, Behzadi P Tags: Acta Microbiol Immunol Hung Source Type: research

Synthesis and characterization of some abundant nanoparticles, their antimicrobial and enzyme inhibition activity.
Abstract Although the antimicrobial activity of the engineered nanoparticles (NPs) is well known, the biochemical mechanisms underlying this activity are not clearly understood. Therefore, four NPs with the highest global production, namely SiO2, TiO2, ZnO, and Ag, were synthesized and characterized. The synthesized SiO2, TiO2, ZnO, and Ag NPs exhibit an average size of 11.12, 13.4, 35, and 50 nm, respectively. The antimicrobial activity of the synthesized NPs against bacteria and fungi were also determined. NPs-mediated inhibition of two very important enzymes, namely urease and DNA polymerase, is also repor...
Source: Acta Microbiologica et Immunologica Hungarica - February 19, 2017 Category: Microbiology Authors: Khan ST, Malik A, Wahab R, Abd-Elkader OH, Ahamed M, Ahmad J, Musarrat J, Siddiqui MA, Al-Khedhairy AA Tags: Acta Microbiol Immunol Hung Source Type: research

Novel adenovirus detected in kowari (Dasyuroides byrnei) with pneumonia.
V, Bányai K, Farkas SL Abstract A male kowari (Dasyuroides byrnei) originating from a zoo facility was delivered for post mortem evaluation in Hungary. Acute lobar pneumonia with histopathologic changes resembling an adenovirus (AdV) infection was detected by light microscopic examination. The presence of an AdV was confirmed by obtaining partial sequence data from the adenoviral DNA-dependent DNA-polymerase. Although the exact taxonomic position of this novel marsupial origin virus could not be determined, pairwise identity analyses and phylogenetic calculations revealed that it is distantly related to oth...
Source: Acta Microbiologica et Immunologica Hungarica - February 14, 2017 Category: Microbiology Authors: Gál J, Mándoki M, Sós E, Kertész P, Koroknai V, Bányai K, Farkas SL Tags: Acta Microbiol Immunol Hung Source Type: research

Molecular detection of aminoglycoside-modifying enzyme genes in Acinetobacter baumannii clinical isolates.
This study showed a high prevalence rate of AME genes in A. baumannii. This prevalence rate has explained that further aminoglycoside resistance genes may have role in the resistance of clinical isolates of A. baumannii. Therefore, control and treatment of serious infections caused by this opportunistic pathogen should be given more consideration. PMID: 27981851 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - December 15, 2016 Category: Microbiology Authors: Heidary M, Salimi Chirani A, Khoshnood S, Eslami G, Atyabi SM, Nazem H, Fazilati M, Hashemi A, Soleimani S Tags: Acta Microbiol Immunol Hung Source Type: research

Antimicrobial resistance in Enterococcus strains isolated from healthy domestic dogs.
Abstract Enterococci are opportunistic bacteria that cause severe infections in animals and humans, capable to acquire, express, and transfer antimicrobial resistance. Susceptibility to 21 antimicrobial agents was tested by the disk diffusion method in 222 Enterococcus spp. strains isolated from the fecal samples of 287 healthy domestic dogs. Vancomycin and ampicillin minimum inhibitory concentrations (MICs) and high-level aminoglycoside resistance (HLAR) tests were also performed. Isolates showed resistance mainly to streptomycin (88.7%), neomycin (80.6%), and tetracycline (69.4%). Forty-two (18.9%) isolates show...
Source: Acta Microbiologica et Immunologica Hungarica - December 14, 2016 Category: Microbiology Authors: Bertelloni F, Salvadori C, Lotti G, Cerri D, Ebani VV Tags: Acta Microbiol Immunol Hung Source Type: research

Detection of acrA, acrB, aac(6')-Ib-cr, and qepA genes among clinical isolates of Escherichia coli and Klebsiella pneumoniae.
CONCLUSION: The distribution of qepA, aac(6')-Ib-cr, acrA, and acrB resistance determinants in E. coli and K. pneumoniae is a great concern. Therefore, infection control and prevention of spread of drug-resistant bacteria need careful management of medication and identification of resistant isolates. PMID: 27936860 [PubMed - as supplied by publisher] (Source: Acta Microbiologica et Immunologica Hungarica)
Source: Acta Microbiologica et Immunologica Hungarica - December 11, 2016 Category: Microbiology Authors: Heidary M, Bahramian A, Hashemi A, Goudarzi M, Omrani VF, Eslami G, Goudarzi H Tags: Acta Microbiol Immunol Hung Source Type: research

Effects of hemin, CO2, and pH on the branching of Candida albicans filamentous forms.
J, Pócsi I Abstract Morphological transitions of wild-type and oxidative stress-tolerant Candida albicans strains were followed in the RPMI-FBS culture medium at pH values and CO2 levels characteristic for the anatomical niches inhabited by this opportunistic human pathogen fungus, including the oral cavity as well as the intestinal and vaginal lumens. Selected cultures were also supplemented with hemin modeling bleedings. Germination as well as elongation and branching of hyphae were monitored in the cultures using time-lapse video microscopy. Unexpectedly, branching time, which is defined as the time take...
Source: Acta Microbiologica et Immunologica Hungarica - December 11, 2016 Category: Microbiology Authors: Jakab Á, Antal K, Emri T, Boczonádi I, Imre A, Gebri E, Majoros L, Pfliegler WP, Szarka M, Balla G, Balla J, Pócsi I Tags: Acta Microbiol Immunol Hung Source Type: research

Full-length VP2 gene analysis of canine parvovirus reveals emergence of newer variants in India.
Abstract The canine parvovirus (CPV) infection is a highly contagious and serious enteric disease of dogs with high fatality rate. The present study was taken up to characterize the full-length viral polypeptide 2 (VP2) gene of CPV of Indian origin along with the commercially available vaccines. The faecal samples from parvovirus suspected dogs were collected from various states of India for screening by PCR assay and 66.29% of samples were found positive. Six CPV-2a, three CPV-2b, and one CPV-2c types were identified by sequence analysis. Several unique and existing mutations have been noticed in CPV types analyz...
Source: Acta Microbiologica et Immunologica Hungarica - December 11, 2016 Category: Microbiology Authors: Nookala M, Mukhopadhyay HK, Sivaprakasam A, Balasubramanian B, Antony PX, Thanislass J, Srinivas MV, Pillai RM Tags: Acta Microbiol Immunol Hung Source Type: research

Composition of endophytic fungal community associated with leaves of maize cultivated in south Brazilian field.
The objective of this study was to conduct a survey about fungi associated with leaves from two different maize plant lineages and to analyze their microbiota diversity. Isolated fungi were identified by morphological analysis and molecular taxonomy was performed using ITS1-5.8S-ITS2 rDNA. About 27 fungi morphotypes were obtained, 15 of them were from the first maize lineage. About 86.7% of the individuals belonged to the Dothideomycetes class (Phoma sorghina, Epicocum nigrum, Cladosporium sp., Bipolaris zeicola, and Alternaria alternata complex) and 13.3% to the Sordariomycetes class (Diaporthe/Phomopsis sp. and Nigrospor...
Source: Acta Microbiologica et Immunologica Hungarica - December 11, 2016 Category: Microbiology Authors: Szilagyi-Zecchin VJ, Adamoski D, Gomes RR, Hungria M, Ikeda AC, Kava-Cordeiro V, Glienke C, Galli-Terasawa LV Tags: Acta Microbiol Immunol Hung Source Type: research

The in vitro effect of Garden Thyme (Thymus vulgaris L.) extract on Brachyspira hyodysenteriae.
Abstract Filtrates of a water extract of commercially available garden thyme (Thymus vulgaris L.) were used for studying its possible bactericidal effect on Brachyspira hyodysenteriae, the causative agent of swine dysentery, by agar-diffusion technique. Five of the six studied Brachyspira strains have proven to be sensitive and one moderately sensitive in the in vitro tests. It was concluded that water extract of garden thyme possesses inhibitory effects against B. hyodysenteriae. In vivo experiments are needed to check the validity of this conclusion. PMID: 27929352 [PubMed - as supplied by publisher] (Sourc...
Source: Acta Microbiologica et Immunologica Hungarica - December 7, 2016 Category: Microbiology Authors: Kutasi J, Jakab L, Jurkovich V, Rafai P Tags: Acta Microbiol Immunol Hung Source Type: research

Enhanced survival but not amplification of Francisella spp. in the presence of free-living amoebae.
In this study, axenic FLA cultures of Acanthamoeba castellanii, Acanthamoeba polyphaga, and Vermamoeba vermiformis were each inoculated with virulent strains of F. tularensis (Types A and B), the attenuated live vaccine strain, and Francisella novicida. Experimental parameters included low and high multiplicity of infection and incubation temperatures of 25 and 30 °C for 0-10 days. Francisella spp. survival was enhanced by the presence of FLA; however, bacterial growth and protozoa infectivity were not observed. In contrast, co-infections of A. polyphaga and Legionella pneumophila, used as an amoeba pathogen control, r...
Source: Acta Microbiologica et Immunologica Hungarica - December 7, 2016 Category: Microbiology Authors: Buse HY, Schaefer FW, Rice EW Tags: Acta Microbiol Immunol Hung Source Type: research

Differences in MRSA prevalence and resistance patterns in a tertiary center before and after joining an international program for surveillance of antimicrobial resistance.
Abstract Methicillin-resistant Staphylococcus aureus (MRSA) emerged as one of the most important causes of hospital-acquired bloodstream infections (BSIs), especially the multidrug resistant clones. The aim of the present study was to compare prevalence and resistance patterns of MRSA bacteremia in the major tertiary-care academic and referral center in Serbia before and after implementing an active antimicrobial resistance (AMR) surveillance. Laboratory-based before-after study was conducted during a two-year period (January 2012 to December 2013) in Clinical Centre of Serbia. Isolation and identification of bact...
Source: Acta Microbiologica et Immunologica Hungarica - December 7, 2016 Category: Microbiology Authors: Djuric O, Jovanovic S, Stosovic B, Tosic T, Jovanovic M, Nartey N, Todorovic J, Markovic-Denic L Tags: Acta Microbiol Immunol Hung Source Type: research