Rapid detection of carbapenemase-producing gram-negative bacteria directly from blood culture bottles for a reliable guided empiric therapy
This study aimed to evaluate the time required for rapid detection of carbapenemase-producing Enterobacterales (CPE) directly from blood culture bottles to optimize empirical treatment of BSI, especially in pediatric and infant patients, using a cost-effective method. This study included 419 Gram-negative bacteria, of which Klebsiella pneumoniae and Escherichia coli were the most common CPE causing BSI in pediatric and neonatal patients. Phenotypic and genotypic resistance of the selected isolates (45 K. pneumoniae and 9 E. coli) were determined by VITEK-2 Compact system and PCR, respectively. BACT/ALERT bottles were spike...
Source: Acta Microbiologica et Immunologica Hungarica - April 4, 2024 Category: Microbiology Authors: Shahinda Rezk Nourhan Ashraf Hassan Heshmat Gamal Elsawaf Amel Elsheredy Source Type: research

Rapid detection of carbapenemase-producing gram-negative bacteria directly from blood culture bottles for a reliable guided empiric therapy
This study aimed to evaluate the time required for rapid detection of carbapenemase-producing Enterobacterales (CPE) directly from blood culture bottles to optimize empirical treatment of BSI, especially in pediatric and infant patients, using a cost-effective method. This study included 419 Gram-negative bacteria, of which Klebsiella pneumoniae and Escherichia coli were the most common CPE causing BSI in pediatric and neonatal patients. Phenotypic and genotypic resistance of the selected isolates (45 K. pneumoniae and 9 E. coli) were determined by VITEK-2 Compact system and PCR, respectively. BACT/ALERT bottles were spike...
Source: Acta Microbiologica et Immunologica Hungarica - April 4, 2024 Category: Microbiology Authors: Shahinda Rezk Nourhan Ashraf Hassan Heshmat Gamal Elsawaf Amel Elsheredy Source Type: research

Rapid detection of carbapenemase-producing gram-negative bacteria directly from blood culture bottles for a reliable guided empiric therapy
This study aimed to evaluate the time required for rapid detection of carbapenemase-producing Enterobacterales (CPE) directly from blood culture bottles to optimize empirical treatment of BSI, especially in pediatric and infant patients, using a cost-effective method. This study included 419 Gram-negative bacteria, of which Klebsiella pneumoniae and Escherichia coli were the most common CPE causing BSI in pediatric and neonatal patients. Phenotypic and genotypic resistance of the selected isolates (45 K. pneumoniae and 9 E. coli) were determined by VITEK-2 Compact system and PCR, respectively. BACT/ALERT bottles were spike...
Source: Acta Microbiologica et Immunologica Hungarica - April 4, 2024 Category: Microbiology Authors: Shahinda Rezk Nourhan Ashraf Hassan Heshmat Gamal Elsawaf Amel Elsheredy Source Type: research

Rapid detection of carbapenemase-producing gram-negative bacteria directly from blood culture bottles for a reliable guided empiric therapy
This study aimed to evaluate the time required for rapid detection of carbapenemase-producing Enterobacterales (CPE) directly from blood culture bottles to optimize empirical treatment of BSI, especially in pediatric and infant patients, using a cost-effective method. This study included 419 Gram-negative bacteria, of which Klebsiella pneumoniae and Escherichia coli were the most common CPE causing BSI in pediatric and neonatal patients. Phenotypic and genotypic resistance of the selected isolates (45 K. pneumoniae and 9 E. coli) were determined by VITEK-2 Compact system and PCR, respectively. BACT/ALERT bottles were spike...
Source: Acta Microbiologica et Immunologica Hungarica - April 4, 2024 Category: Microbiology Authors: Shahinda Rezk Nourhan Ashraf Hassan Heshmat Gamal Elsawaf Amel Elsheredy Source Type: research

Phylogenetic analysis and antimony resistance of Leishmania major isolated from humans and rodents
Acta Microbiol Immunol Hung. 2024 Mar 21;71(1):89-98. doi: 10.1556/030.2024.02194. Print 2024 Mar 26.ABSTRACTCutaneous Leishmaniasis (CL) is one of the world's neglected diseases which is caused by Leishmania spp. The aim of this study was to assess molecular profile and antimony resistance of Leishmania isolated from human and rodent hosts. Samples were collected from suspected CL patients referred to health centres and wild rodent's traps in Gonbad-e-Qabus region, north-eastern Iran. Smears were subjected to PCR-RFLP to identify Leishmania species. In addition, ITS1-PCR products were sequenced for phylogenetic analysis. ...
Source: Acta Microbiologica et Immunologica Hungarica - March 23, 2024 Category: Microbiology Authors: Yussef Moghaddam Hajar Ziaei Hezarjaribi Abdol Sattar Pagheh Mahdi Fakhar Reza Saberi Mitra Sharbatkhori Mahbobeh Montazeri Hossein Ghalehnoei Eisa Nazar Source Type: research

Phylogenetic analysis and antimony resistance of Leishmania major isolated from humans and rodents
Acta Microbiol Immunol Hung. 2024 Mar 21;71(1):89-98. doi: 10.1556/030.2024.02194. Print 2024 Mar 26.ABSTRACTCutaneous Leishmaniasis (CL) is one of the world's neglected diseases which is caused by Leishmania spp. The aim of this study was to assess molecular profile and antimony resistance of Leishmania isolated from human and rodent hosts. Samples were collected from suspected CL patients referred to health centres and wild rodent's traps in Gonbad-e-Qabus region, north-eastern Iran. Smears were subjected to PCR-RFLP to identify Leishmania species. In addition, ITS1-PCR products were sequenced for phylogenetic analysis. ...
Source: Acta Microbiologica et Immunologica Hungarica - March 23, 2024 Category: Microbiology Authors: Yussef Moghaddam Hajar Ziaei Hezarjaribi Abdol Sattar Pagheh Mahdi Fakhar Reza Saberi Mitra Sharbatkhori Mahbobeh Montazeri Hossein Ghalehnoei Eisa Nazar Source Type: research

Phylogenetic analysis and antimony resistance of Leishmania major isolated from humans and rodents
Acta Microbiol Immunol Hung. 2024 Mar 21;71(1):89-98. doi: 10.1556/030.2024.02194. Print 2024 Mar 26.ABSTRACTCutaneous Leishmaniasis (CL) is one of the world's neglected diseases which is caused by Leishmania spp. The aim of this study was to assess molecular profile and antimony resistance of Leishmania isolated from human and rodent hosts. Samples were collected from suspected CL patients referred to health centres and wild rodent's traps in Gonbad-e-Qabus region, north-eastern Iran. Smears were subjected to PCR-RFLP to identify Leishmania species. In addition, ITS1-PCR products were sequenced for phylogenetic analysis. ...
Source: Acta Microbiologica et Immunologica Hungarica - March 23, 2024 Category: Microbiology Authors: Yussef Moghaddam Hajar Ziaei Hezarjaribi Abdol Sattar Pagheh Mahdi Fakhar Reza Saberi Mitra Sharbatkhori Mahbobeh Montazeri Hossein Ghalehnoei Eisa Nazar Source Type: research

Phylogenetic analysis and antimony resistance of Leishmania major isolated from humans and rodents
Acta Microbiol Immunol Hung. 2024 Mar 21;71(1):89-98. doi: 10.1556/030.2024.02194. Print 2024 Mar 26.ABSTRACTCutaneous Leishmaniasis (CL) is one of the world's neglected diseases which is caused by Leishmania spp. The aim of this study was to assess molecular profile and antimony resistance of Leishmania isolated from human and rodent hosts. Samples were collected from suspected CL patients referred to health centres and wild rodent's traps in Gonbad-e-Qabus region, north-eastern Iran. Smears were subjected to PCR-RFLP to identify Leishmania species. In addition, ITS1-PCR products were sequenced for phylogenetic analysis. ...
Source: Acta Microbiologica et Immunologica Hungarica - March 23, 2024 Category: Microbiology Authors: Yussef Moghaddam Hajar Ziaei Hezarjaribi Abdol Sattar Pagheh Mahdi Fakhar Reza Saberi Mitra Sharbatkhori Mahbobeh Montazeri Hossein Ghalehnoei Eisa Nazar Source Type: research

Phylogenetic analysis and antimony resistance of Leishmania major isolated from humans and rodents
Acta Microbiol Immunol Hung. 2024 Mar 21;71(1):89-98. doi: 10.1556/030.2024.02194. Print 2024 Mar 26.ABSTRACTCutaneous Leishmaniasis (CL) is one of the world's neglected diseases which is caused by Leishmania spp. The aim of this study was to assess molecular profile and antimony resistance of Leishmania isolated from human and rodent hosts. Samples were collected from suspected CL patients referred to health centres and wild rodent's traps in Gonbad-e-Qabus region, north-eastern Iran. Smears were subjected to PCR-RFLP to identify Leishmania species. In addition, ITS1-PCR products were sequenced for phylogenetic analysis. ...
Source: Acta Microbiologica et Immunologica Hungarica - March 23, 2024 Category: Microbiology Authors: Yussef Moghaddam Hajar Ziaei Hezarjaribi Abdol Sattar Pagheh Mahdi Fakhar Reza Saberi Mitra Sharbatkhori Mahbobeh Montazeri Hossein Ghalehnoei Eisa Nazar Source Type: research

Phylogenetic analysis and antimony resistance of Leishmania major isolated from humans and rodents
Acta Microbiol Immunol Hung. 2024 Mar 21;71(1):89-98. doi: 10.1556/030.2024.02194. Print 2024 Mar 26.ABSTRACTCutaneous Leishmaniasis (CL) is one of the world's neglected diseases which is caused by Leishmania spp. The aim of this study was to assess molecular profile and antimony resistance of Leishmania isolated from human and rodent hosts. Samples were collected from suspected CL patients referred to health centres and wild rodent's traps in Gonbad-e-Qabus region, north-eastern Iran. Smears were subjected to PCR-RFLP to identify Leishmania species. In addition, ITS1-PCR products were sequenced for phylogenetic analysis. ...
Source: Acta Microbiologica et Immunologica Hungarica - March 23, 2024 Category: Microbiology Authors: Yussef Moghaddam Hajar Ziaei Hezarjaribi Abdol Sattar Pagheh Mahdi Fakhar Reza Saberi Mitra Sharbatkhori Mahbobeh Montazeri Hossein Ghalehnoei Eisa Nazar Source Type: research

Phylogenetic analysis and antimony resistance of Leishmania major isolated from humans and rodents
Acta Microbiol Immunol Hung. 2024 Mar 21;71(1):89-98. doi: 10.1556/030.2024.02194. Print 2024 Mar 26.ABSTRACTCutaneous Leishmaniasis (CL) is one of the world's neglected diseases which is caused by Leishmania spp. The aim of this study was to assess molecular profile and antimony resistance of Leishmania isolated from human and rodent hosts. Samples were collected from suspected CL patients referred to health centres and wild rodent's traps in Gonbad-e-Qabus region, north-eastern Iran. Smears were subjected to PCR-RFLP to identify Leishmania species. In addition, ITS1-PCR products were sequenced for phylogenetic analysis. ...
Source: Acta Microbiologica et Immunologica Hungarica - March 23, 2024 Category: Microbiology Authors: Yussef Moghaddam Hajar Ziaei Hezarjaribi Abdol Sattar Pagheh Mahdi Fakhar Reza Saberi Mitra Sharbatkhori Mahbobeh Montazeri Hossein Ghalehnoei Eisa Nazar Source Type: research

Phylogenetic analysis and antimony resistance of Leishmania major isolated from humans and rodents
Acta Microbiol Immunol Hung. 2024 Mar 21. doi: 10.1556/030.2024.02194. Online ahead of print.ABSTRACTCutaneous Leishmaniasis (CL) is one of the world's neglected diseases which is caused by Leishmania spp. The aim of this study was to assess molecular profile and antimony resistance of Leishmania isolated from human and rodent hosts. Samples were collected from suspected CL patients referred to health centres and wild rodent's traps in Gonbad-e-Qabus region, north-eastern Iran. Smears were subjected to PCR-RFLP to identify Leishmania species. In addition, ITS1-PCR products were sequenced for phylogenetic analysis. Clinical...
Source: Acta Microbiologica et Immunologica Hungarica - March 23, 2024 Category: Microbiology Authors: Yussef Moghaddam Hajar Ziaei Hezarjaribi Abdol Sattar Pagheh Mahdi Fakhar Reza Saberi Mitra Sharbatkhori Mahbobeh Montazeri Hossein Ghalehnoei Eisa Nazar Source Type: research

Phylogenetic analysis and antimony resistance of Leishmania major isolated from humans and rodents
Acta Microbiol Immunol Hung. 2024 Mar 21. doi: 10.1556/030.2024.02194. Online ahead of print.ABSTRACTCutaneous Leishmaniasis (CL) is one of the world's neglected diseases which is caused by Leishmania spp. The aim of this study was to assess molecular profile and antimony resistance of Leishmania isolated from human and rodent hosts. Samples were collected from suspected CL patients referred to health centres and wild rodent's traps in Gonbad-e-Qabus region, north-eastern Iran. Smears were subjected to PCR-RFLP to identify Leishmania species. In addition, ITS1-PCR products were sequenced for phylogenetic analysis. Clinical...
Source: Acta Microbiologica et Immunologica Hungarica - March 23, 2024 Category: Microbiology Authors: Yussef Moghaddam Hajar Ziaei Hezarjaribi Abdol Sattar Pagheh Mahdi Fakhar Reza Saberi Mitra Sharbatkhori Mahbobeh Montazeri Hossein Ghalehnoei Eisa Nazar Source Type: research

Occupational exposure to Coxiella burnetii during cardiac surgery: A case report and review of the literature
We report a case of exposure to Coxiella burnetii in a surgical nurse who underwent an injury of her finger with a scalpel blade during a native aortic valve replacement with a bio-prosthetic cardiac valve conducted on a patient suffering from C. burnetii aortic endocarditis. Given the positivity of C. burnetii culture and PCR from the patient's aortic valve, she was prescribed prophylactic doxycycline 100 mg twice a day for 10 days. Q fever is an occupational zoonosis resulting usually of exposure to infected animals by inhalation of infected aerosols or consumption of contaminated raw milk. Apart from materno-foetal tran...
Source: Acta Microbiologica et Immunologica Hungarica - March 11, 2024 Category: Microbiology Authors: Patient Diwunga Matthieu Million Isabelle Ravaux Pierre Edouard Fournier Philippe Gautret Source Type: research

Occupational exposure to Coxiella burnetii during cardiac surgery: A case report and review of the literature
We report a case of exposure to Coxiella burnetii in a surgical nurse who underwent an injury of her finger with a scalpel blade during a native aortic valve replacement with a bio-prosthetic cardiac valve conducted on a patient suffering from C. burnetii aortic endocarditis. Given the positivity of C. burnetii culture and PCR from the patient's aortic valve, she was prescribed prophylactic doxycycline 100 mg twice a day for 10 days. Q fever is an occupational zoonosis resulting usually of exposure to infected animals by inhalation of infected aerosols or consumption of contaminated raw milk. Apart from materno-foetal tran...
Source: Acta Microbiologica et Immunologica Hungarica - March 11, 2024 Category: Microbiology Authors: Patient Diwunga Matthieu Million Isabelle Ravaux Pierre Edouard Fournier Philippe Gautret Source Type: research