Prevalence of antibiotic resistance in clinical isolates of < em > Mycobacterium kansasii < /em > : a systematic review and meta-analysis
Expert Rev Anti Infect Ther. 2024 Jan 31:1-11. doi: 10.1080/14787210.2024.2313051. Online ahead of print.ABSTRACTINTRODUCTION: The prevalence of diseases caused by non-tuberculous mycobacteria (NTM), including M. kansasii, is increasing, necessitating further information to guide prevention, control, and treatment strategies.AREAS COVERED: A comprehensive analysis of articles published until February 2023 was conducted on PubMed, Web of Science, and Scopus databases to investigate antibiotic resistance in M. kansasii species. Stata software version 17 was employed for all analyses.EXPERT OPINION: A total of 1647 articles w...
Source: Expert Review of Anti-Infective Therapy - January 31, 2024 Category: Infectious Diseases Authors: Negar Narimisa Forough Goodarzi Narjess Bostanghadiri Faramarz Masjedian Jazi Source Type: research
Antibiomania: clarithromycin-induced neurotoxicity mimicking autoimmune limbic encephalitis
We describe a 64-year-old woman with relapsing encephalopathy. She initially presented with 5 days of psychomotor agitation, progressing to mania, psychosis and seizures that mimicked autoimmune limbic encephalitis. During her first hospital admission, extensive investigation failed to establish the underlying cause, and she improved with antiseizure medication alone. After a month at home, she relapsed with identical symptoms, and only then did we recognise that both episodes had been provoked by clarithromycin, prescribed for Helicobacter pylori eradication. Clarithromycin-induced neurotoxicity is rarely reported but lik...
Source: Practical Neurology - January 30, 2024 Category: Neurology Authors: Daniel Whittam Rachael Matthews Randa Nimeri Saifuddin Shaik Source Type: research
Antibiotic resistance and genomic characterization of Mycobacterium abscessus complex isolates from patients with pulmonary tuberculosis in Iran: A multi-center study (2010-2021)
Acta Microbiol Immunol Hung. 2024 Jan 29. doi: 10.1556/030.2024.02169. Online ahead of print.ABSTRACTOne hundred and eighteen sputum specimens suspected of Mycobacterium abscessus infection were collected. Species level identification of M. abscessus was performed by rpoB sequencing. Clonality analysis was done by multilocus sequence typing (MLST) for M. abscessus. Antibiotic susceptibility testing was performed for clarithromycin, amikacin, ciprofloxacin and moxifloxacin. Altogether 128 isolates were obtained and were subjected to rpoB gene sequencing for definite identification. Among them 59 were identified as M. absces...
Source: Acta Microbiologica et Immunologica Hungarica - January 29, 2024 Category: Microbiology Authors: Mohammad Hashemzadeh Aram Asareh Zadegan Dezfuli AzarDokht Khosravi Nazanin Ahmad Khosravi Morteza Saki Source Type: research
Mycobacterium abscessus extracellular vesicles increase mycobacterial resistance to clarithromycin in vitro
In this study, we characterized the extracellular vesicles (EVs) released by drug-sensitiveM. abscessus untreated or treated with clarithromycin (CLR), one of the frontline anti-NTM drugs. Our data show that exposure to CLR increases mycobacterial protein trafficking into EVs as well as the secretion of EVs in culture. Additionally, EVs released by CLR-treatedM. abscessus increaseM. abscessus resistance to CLR when compared to EVs from untreatedM. abscessus. Proteomic analysis further indicates that EVs released by CLR-treatedM. abscessus carry an increased level of 50S ribosomal subunits, the target of CLR. Taken together...
Source: Proteomics - January 19, 2024 Category: Biochemistry Authors: Charlie A. Vermeire,
Xuejuan Tan,
Yurong Liang,
Stephen K. Kotey,
Janet Rogers,
Steven D. Hartson,
Lin Liu,
Yong Cheng Tags: RESEARCH ARTICLE Source Type: research
Genomic and vaccine preclinical studies reveal a novel mouse-adapted < em > Helicobacter pylori < /em > model for the hpEastAsia genotype in Southeast Asia
Conclusion. Our findings demonstrate the successful development of a novel mouse-adapted H. pylori model for the hpEastAsia genotype in Vietnam and Southeast Asia. Our research highlights the distinctive genotype and pathogenicity of clinical H. pylori strains in the region, laying the foundation for targeted interventions to address this global health burden.PMID:38235783 | DOI:10.1099/jmm.0.001786 (Source: Journal of Medical Microbiology)
Source: Journal of Medical Microbiology - January 18, 2024 Category: Microbiology Authors: Thi Kim Cuc Nguyen Hoang Dang Khoa Do Thi Lan Phuong Nguyen Thu Thuy Pham Bao Ngoc Mach Thi Chinh Nguyen Thi Lan Pham Paidamoyo M Katsande Huynh Anh Hong Huu Thai Duong Anh N Phan Simon M Cutting Minh Thiet Vu Van Duy Nguyen Source Type: research
Antibiotic resistance in Helicobacter pylori among children and adolescents in East Asia: A systematic review and meta-analysis
CONCLUSIONS: H. pylori isolates from East Asian children and adolescents exhibit high levels of metronidazole and clarithromycin resistance, particularly in the Chinese mainland. The non-negligible rates of dual and multiple resistance highlight the complexity of this problem.REGISTRATION: PROSPERO, No. IDCRD42023402510.PMID:38230488 | DOI:10.1097/CM9.0000000000002884 (Source: Chinese Medical Journal)
Source: Chinese Medical Journal - January 17, 2024 Category: General Medicine Authors: Yuhang Zhou Yanli Zhang Shiyu Du Source Type: research
Sustain-release lipid-liquid crystal formulations of pexiganan against Helicobacter pylori infection: in vitro evaluation in C57BL/6 mice
CONCLUSION: Clarithromycin was more effective than pexiganan at all tested concentrations. Furthermore, the pexiganan-loaded LLCs exhibited superior efficacy in curing H. pylori infection in a mouse model compared to pexiganan alone. This formulation can enhance H. pylori clearance while mitigating the adverse effects, typically associated with conventional drugs, leading to a viable alternative to current treatment options.PMID:38212864 | PMC:PMC10785446 | DOI:10.1186/s40360-024-00731-z (Source: BMC Pharmacology and Toxicology)
Source: BMC Pharmacology and Toxicology - January 11, 2024 Category: Drugs & Pharmacology Authors: Kiarash Ghazvini Hossein Kamali Hadi Farsiani Masoud Yousefi Masoud Keikha Source Type: research
Streptomyces albireticuli lung infection managed as a pulmonary air cyst: a case report and literature review
Streptomyces, the largest genus in the Streptomycetaceae family and a prolific producer of antibacterial drugs, is a saprophytic soil organism that rarely causes invasive infections. Here we report a case of necrotic pneumonia caused by Streptomyces albireticuli in a 75-year-old man who presented with progressive chest tightness and dyspnea. Streptomyces albireticuli was isolated from his bronchoalveolar lavage fluid and identified through whole-genome sequencing (WGS) and phylogenetic analysis. The patient responded satisfactorily to clarithromycin therapy. The findings of this study may enhance our vigilance in identifyi...
Source: Frontiers in cellular and infection microbiology - January 11, 2024 Category: Microbiology Source Type: research
Clarithromycin for early anti-inflammatory responses in community-acquired pneumonia in Greece (ACCESS): a randomised, double-blind, placebo-controlled trial
Lancet Respir Med. 2024 Jan 3:S2213-2600(23)00412-5. doi: 10.1016/S2213-2600(23)00412-5. Online ahead of print.ABSTRACTBACKGROUND: Addition of macrolide antibiotics to β-lactam antibiotics for the treatment of patients in hospital with community-acquired pneumonia is based on results from observational studies and meta-analyses rather than randomised clinical trials. We investigated if addition of the macrolide clarithromycin to treatment with a β-lactam antibiotic in this population could improve early clinical response-the new regulatory endpoint for community-acquired pneumonia-and explored the possible contribution o...
Source: Respiratory Care - January 6, 2024 Category: Respiratory Medicine Authors: Evangelos J Giamarellos-Bourboulis Athanasios Siampanos Amalia Bolanou Sarantia Doulou Nikolaos Kakavoulis Konstantinos Tsiakos Sokratis Katopodis Georgios Schinas Lamprini Skorda Zoi Alexiou Konstantinos Armenis Paraskevi Katsaounou George Chrysos Aikate Source Type: research
Cut-off value of clarithromycin resistance in the treatment of Helicobacter pylori infection: how low is low?
We read the Maastricht VI/Florence consensus report for the management of Helicobacter pylori (H. pylori) infection by Malfertheiner et al1 published in the Gut with great interest. The experts addressed that the goal of any antimicrobial therapy is to cure reliably H. pylori infection in the majority (eg, ≥90%) of patients. The goal of cure rate ≥90% meets the expectation of patients in the real-world expectation survey of Asia-Pacific patients for H. pylori eradication therapy,2 which showed 91% of accepted minimal eradication rate expected by H. pylori-infected patients. In the consensus report, clarithromycin tri...
Source: Gut - January 5, 2024 Category: Gastroenterology Authors: Hsu, P.-I., Chuah, S.-K., Yamaoka, Y., Wu, D.-C. Tags: Gut PostScript Source Type: research
Deciphering complex antibiotic resistance patterns in Helicobacter pylori through whole genome sequencing and machine learning
In this study, we analyzed 52 Hp strains collected from various hospitals. The susceptibility of these strains to five antibiotics was assessed using the agar dilution assay. Whole-genome sequencing was then performed to screen the antimicrobial resistance (AMR) genotypes of these Hp strains. To model the relationship between drug resistance and genotype, we employed univariate statistical tests, unsupervised machine learning, and supervised machine learning techniques, including the development of support vector machine models.ResultsOur models for predicting Amoxicillin resistance demonstrated 66% sensitivity and 100% sp...
Source: Frontiers in cellular and infection microbiology - January 4, 2024 Category: Microbiology Source Type: research
GSE213117 Dual RNA-sequencing of macrophages infected with Mycobacterium avium reveals host-pathogen interaction in the context of clarithromycin treatment
Conclusions These data identify the distinct stress responses of M. avium upon infection and clarithromycin treatment and underline the importance of taking the intracellular localization and interaction with the host into account when studying antibiotics against intracellular pathogens. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - January 1, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mycobacterium avium subsp. hominissuis Source Type: research
GSE213116 The role of metabolic shifts and drug transporters in the response of M. avium to antibiotic stress
Conclusion: M. avium likely relies on a combination of drug-specific and generic mechanisms of antibiotic tolerance including changes in respiratory and metabolic state in addition to drug efflux and target modification. Efflux inhibition can increase susceptibility but this effect is EPI and antibiotic specific. Finally, the lack of amikacin modifying activity in M. avium is important for its activity. (Source: GEO: Gene Expression Omnibus)
Source: GEO: Gene Expression Omnibus - January 1, 2024 Category: Genetics & Stem Cells Tags: Expression profiling by high throughput sequencing Mycobacterium avium subsp. hominissuis Source Type: research
Simultaneous determination of macrolides in water samples by solid-phase extraction and capillary electrophoresis
Acta Pharm. 2023 Dec 26;73(4):515-535. doi: 10.2478/acph-2023-0041. Print 2023 Dec 1.ABSTRACTSolid-phase extraction (SPE) coupled with capillary electrophoresis (CE) for the determination of macrolide antibiotics (azithromycin, clarithromycin, roxithromycin, tylosin) and tiamulin in water samples was described in this article. These compounds were extracted with different types of sorbents ( Oasis HLB, C18, C8, SDB, and Strata-X) and different masses of sorbents (60 mg, 200 mg, and 500 mg) using different organic solvents (methanol, ethanol, and acetonitrile) and different pH values of water samples (pH 7.00, 8.00, and 9.0...
Source: Acta Pharmaceutica - December 26, 2023 Category: Drugs & Pharmacology Authors: Dragana Mutavd žić Pavlović Sandra Babi ć Mirta Čizmić Miranda Serti ć Tea Pinu šić Source Type: research
