Molecules, Vol. 26, Pages 6251: Phytochemical Profile, Free Radical Scavenging and Anti-Inflammatory Properties of Acalypha Indica Root Extract: Evidence from In Vitro and In Vivo Studies
ikarjuna Korivi
In our in vitro and in vivo studies, we used Acalypha indica root methanolic extract (AIRME), and investigated their free radical scavenging/antioxidant and anti-inflammatory properties. Primarily, phytochemical analysis showed rich content of phenols (70.92 mg of gallic acid/g) and flavonoids (16.01 mg of rutin/g) in AIRME. We then performed HR-LC-MS and GC-MS analyses, and identified 101 and 14 phytochemical compounds, respectively. Among them, ramipril glucuronide (1.563%), antimycin A (1.324%), swietenine (1.134%), quinone (1.152%), oxprenolol (1.118%), choline (0.847%), bumetanide (0.847%) and feno...
Source: Molecules - October 15, 2021 Category: Chemistry Authors: Ravi Sahukari Jyothi Punabaka Shanmugam Bhasha Venkata Subbaiah Ganjikunta Shanmugam Kondeti Ramudu Sathyavelu Reddy Kesireddy Weibing Ye Mallikarjuna Korivi Tags: Article Source Type: research
Restoration of mRNA Expression of Solute Carrier Proteins in Liver of Diet-Induced Obese Mice by Metformin
In this study, we examined Slc gene expression in the liver and colon of diet-induced obese (DIO) mice treated with MET by transcriptomic analysis. There were 939 differentially expressed genes (DEGs) in the liver of DIO mice vs lean mice, which included 34 Slc genes. MET altered 489 DEGs in the liver of DIO mice, in which 23 were Slc genes. Expression of 20 MET-responsive Slc DEGs was confirmed by qRT-PCR, in which 15 Slc genes were altered in DIO mice and their expressions were restored by MET, including Slc2a10, Slc2a13, Slc5a9, Slc6a14, Slc7a9, Slc9a2, Slc9a3, Slc13a2, Slc15a2, Slc26a3, Slc34a2, Slc37a1, Slc44a4, Slc51...
Source: Frontiers in Endocrinology - September 30, 2021 Category: Endocrinology Source Type: research
Loop Diuretics Inhibit Ischemia-Induced Intracellular Ca2+ Overload in Neurons via the Inhibition of Voltage-Gated Ca2+ and Na+ Channels
One consequence of ischemic stroke is disruption of intracellular ionic homeostasis. Intracellular overload of both Na+ and Ca2+ has been linked to neuronal death in this pathophysiological state. The etiology of ionic imbalances resulting from stroke-induced ischemia and acidosis includes the dysregulation of multiple plasma membrane transport proteins, such as increased activity of sodium-potassium-chloride cotransporter-1 (NKCC-1). Experiments using NKCC1 antagonists, bumetanide (BMN) and ethacrynic acid (EA), were carried out to determine if inhibition of this cotransporter affects Na+ and Ca2+ overload observed follow...
Source: Frontiers in Pharmacology - September 15, 2021 Category: Drugs & Pharmacology Source Type: research
Revealing the Angiopathy of Lacrimal Gland Lesion in Type 2 Diabetes
For a better understanding of diabetic angiopathy (DA), the potential biomarkers in lacrimal DA and its potential mechanism, we evaluated the morphological and hemodynamic alterations of lacrimal glands (LGs) in patients with type 2 diabetes and healthy counterparts by color Doppler flow imaging (CDFI). We further established a type 2 diabetic mice model and performed hematoxylin-eosin (HE) staining, immunofluorescence staining of CD31, RNA-sequencing analysis, and connectivity map (CMap) analysis. We found atrophy and ischemia in patients with type 2 diabetes and mice models. Furthermore, we identified 846 differentially ...
Source: Frontiers in Physiology - August 31, 2021 Category: Physiology Source Type: research
Gene signatures and potential therapeutic targets of Middle East respiratory syndrome coronavirus (MERS-CoV)-infected human lung adenocarcinoma epithelial cells
CONCLUSIONS: The present study illustrates the crucial roles of miRNA-mRNA interacting networks in MERS-CoV-infected cells. The genes we identified are potential targets for treating MERS-CoV infection; however, these could possibly be extended to other coronavirus infections.PMID:34176764 | PMC:PMC7997684 | DOI:10.1016/j.jmii.2021.03.007 (Source: Journal of Microbiology, Immunology, and Infection)
Source: Journal of Microbiology, Immunology, and Infection - June 28, 2021 Category: Microbiology Authors: Yen-Hung Wu I-Jeng Yeh Nam Nhut Phan Meng-Chi Yen Jui-Hsiang Hung Chung-Chieh Chiao Chien-Fu Chen Zhengda Sun Hui-Ping Hsu Chih-Yang Wang Ming-Derg Lai Source Type: research
Gene signatures and potential therapeutic targets of Middle East respiratory syndrome coronavirus (MERS-CoV)-infected human lung adenocarcinoma epithelial cells
CONCLUSIONS: The present study illustrates the crucial roles of miRNA-mRNA interacting networks in MERS-CoV-infected cells. The genes we identified are potential targets for treating MERS-CoV infection; however, these could possibly be extended to other coronavirus infections.PMID:34176764 | PMC:PMC7997684 | DOI:10.1016/j.jmii.2021.03.007 (Source: Journal of Microbiology, Immunology, and Infection)
Source: Journal of Microbiology, Immunology, and Infection - June 28, 2021 Category: Microbiology Authors: Yen-Hung Wu I-Jeng Yeh Nam Nhut Phan Meng-Chi Yen Jui-Hsiang Hung Chung-Chieh Chiao Chien-Fu Chen Zhengda Sun Hui-Ping Hsu Chih-Yang Wang Ming-Derg Lai Source Type: research
Gene signatures and potential therapeutic targets of Middle East respiratory syndrome coronavirus (MERS-CoV)-infected human lung adenocarcinoma epithelial cells
CONCLUSIONS: The present study illustrates the crucial roles of miRNA-mRNA interacting networks in MERS-CoV-infected cells. The genes we identified are potential targets for treating MERS-CoV infection; however, these could possibly be extended to other coronavirus infections.PMID:34176764 | PMC:PMC7997684 | DOI:10.1016/j.jmii.2021.03.007 (Source: Journal of Microbiology, Immunology, and Infection)
Source: Journal of Microbiology, Immunology, and Infection - June 28, 2021 Category: Microbiology Authors: Yen-Hung Wu I-Jeng Yeh Nam Nhut Phan Meng-Chi Yen Jui-Hsiang Hung Chung-Chieh Chiao Chien-Fu Chen Zhengda Sun Hui-Ping Hsu Chih-Yang Wang Ming-Derg Lai Source Type: research
Gene signatures and potential therapeutic targets of Middle East respiratory syndrome coronavirus (MERS-CoV)-infected human lung adenocarcinoma epithelial cells
CONCLUSIONS: The present study illustrates the crucial roles of miRNA-mRNA interacting networks in MERS-CoV-infected cells. The genes we identified are potential targets for treating MERS-CoV infection; however, these could possibly be extended to other coronavirus infections.PMID:34176764 | DOI:10.1016/j.jmii.2021.03.007 (Source: Journal of Microbiology, Immunology, and Infection)
Source: Journal of Microbiology, Immunology, and Infection - June 28, 2021 Category: Microbiology Authors: Yen-Hung Wu I-Jeng Yeh Nam Nhut Phan Meng-Chi Yen Jui-Hsiang Hung Chung-Chieh Chiao Chien-Fu Chen Zhengda Sun Hui-Ping Hsu Chih-Yang Wang Ming-Derg Lai Source Type: research
Pathology-selective antiepileptic effects in the focal freeze-lesion rat model of malformation of cortical development
Exp Neurol. 2021 May 28:113776. doi: 10.1016/j.expneurol.2021.113776. Online ahead of print.ABSTRACTMalformations of cortical development (MCD) represent a group of rare diseases with severe clinical presentation as epileptic and pharmacoresistant encephalopathies. Morphological studies in tissue from MCD patients have revealed reduced GABAergic efficacy and increased intracellular chloride concentration in neuronal cells as important pathophysiological mechanisms in MCD. Also, in various animal models, alterations of GABAergic inhibition have been postulated as a predominant factor contributing to perilesional hyperexcita...
Source: Experimental Neurology - May 31, 2021 Category: Neurology Authors: Ali énor Ragot Heiko J Luhmann Matthias Dipper-Wawra Uwe Heinemann Martin Holtkamp Pawel Fidzinski Source Type: research
Pathology-selective antiepileptic effects in the focal freeze-lesion rat model of malformation of cortical development
Exp Neurol. 2021 May 28:113776. doi: 10.1016/j.expneurol.2021.113776. Online ahead of print.ABSTRACTMalformations of cortical development (MCD) represent a group of rare diseases with severe clinical presentation as epileptic and pharmacoresistant encephalopathies. Morphological studies in tissue from MCD patients have revealed reduced GABAergic efficacy and increased intracellular chloride concentration in neuronal cells as important pathophysiological mechanisms in MCD. Also, in various animal models, alterations of GABAergic inhibition have been postulated as a predominant factor contributing to perilesional hyperexcita...
Source: Experimental Neurology - May 31, 2021 Category: Neurology Authors: Ali énor Ragot Heiko J Luhmann Matthias Dipper-Wawra Uwe Heinemann Martin Holtkamp Pawel Fidzinski Source Type: research
Pathology-selective antiepileptic effects in the focal freeze-lesion rat model of malformation of cortical development
Exp Neurol. 2021 May 28:113776. doi: 10.1016/j.expneurol.2021.113776. Online ahead of print.ABSTRACTMalformations of cortical development (MCD) represent a group of rare diseases with severe clinical presentation as epileptic and pharmacoresistant encephalopathies. Morphological studies in tissue from MCD patients have revealed reduced GABAergic efficacy and increased intracellular chloride concentration in neuronal cells as important pathophysiological mechanisms in MCD. Also, in various animal models, alterations of GABAergic inhibition have been postulated as a predominant factor contributing to perilesional hyperexcita...
Source: Experimental Neurology - May 31, 2021 Category: Neurology Authors: Ali énor Ragot Heiko J Luhmann Matthias Dipper-Wawra Uwe Heinemann Martin Holtkamp Pawel Fidzinski Source Type: research
Pathology-selective antiepileptic effects in the focal freeze-lesion rat model of malformation of cortical development
Exp Neurol. 2021 May 28:113776. doi: 10.1016/j.expneurol.2021.113776. Online ahead of print.ABSTRACTMalformations of cortical development (MCD) represent a group of rare diseases with severe clinical presentation as epileptic and pharmacoresistant encephalopathies. Morphological studies in tissue from MCD patients have revealed reduced GABAergic efficacy and increased intracellular chloride concentration in neuronal cells as important pathophysiological mechanisms in MCD. Also, in various animal models, alterations of GABAergic inhibition have been postulated as a predominant factor contributing to perilesional hyperexcita...
Source: Experimental Neurology - May 31, 2021 Category: Neurology Authors: Ali énor Ragot Heiko J Luhmann Matthias Dipper-Wawra Uwe Heinemann Martin Holtkamp Pawel Fidzinski Source Type: research
Bumetanide prevents diazepam-modified anxiety-like behavior in lipopolysaccharide-treated mice
Eur J Pharmacol. 2021 May 15:174195. doi: 10.1016/j.ejphar.2021.174195. Online ahead of print.ABSTRACTBenzodiazepine receptor agonists are widely prescribed therapeutic agents that alter gamma-aminobutyric acid (GABA)A receptor activity and have anxiolytic effects. Post-operative use of benzodiazepines is a risk factor of delirium. Inflammatory conditions alter the anxiolytic effects of benzodiazepine. We investigated the effect of diazepam, a typical benzodiazepine anxiolytic, on changes in the emotional behavior of mice in a hole-board test after lipopolysaccharide (LPS) treatment. Diazepam dose-dependently increased the...
Source: European Journal of Pharmacology - May 18, 2021 Category: Drugs & Pharmacology Authors: Daiki Matsumoto Soichiro Ushio Yudai Wada Yukiko Noda Satoru Esumi Yasuhisa Izushi Yoshihisa Kitamura Toshiaki Sendo Source Type: research
Bumetanide prevents diazepam-modified anxiety-like behavior in lipopolysaccharide-treated mice
Eur J Pharmacol. 2021 May 15:174195. doi: 10.1016/j.ejphar.2021.174195. Online ahead of print.ABSTRACTBenzodiazepine receptor agonists are widely prescribed therapeutic agents that alter gamma-aminobutyric acid (GABA)A receptor activity and have anxiolytic effects. Post-operative use of benzodiazepines is a risk factor of delirium. Inflammatory conditions alter the anxiolytic effects of benzodiazepine. We investigated the effect of diazepam, a typical benzodiazepine anxiolytic, on changes in the emotional behavior of mice in a hole-board test after lipopolysaccharide (LPS) treatment. Diazepam dose-dependently increased the...
Source: European Journal of Pharmacology - May 18, 2021 Category: Drugs & Pharmacology Authors: Daiki Matsumoto Soichiro Ushio Yudai Wada Yukiko Noda Satoru Esumi Yasuhisa Izushi Yoshihisa Kitamura Toshiaki Sendo Source Type: research
The role of HCO < sub > 3 < /sub > < sup > - < /sup > in propionate-induced anion secretion across rat caecal epithelium
Pflugers Arch. 2021 Apr 29. doi: 10.1007/s00424-021-02565-8. Online ahead of print.ABSTRACTPropionate, a metabolite from the microbial fermentation of carbohydrates, evokes a release of epithelial acetylcholine in rat caecum resulting in an increase of short-circuit current (Isc) in Ussing chamber experiments. The present experiments were performed in order to characterize the ionic mechanisms underlying this response which has been thought to be due to Cl- secretion. As there are regional differences within the caecal epithelium, the experiments were conducted at oral and aboral rat corpus caeci. In both caecal segments, ...
Source: Pflugers Archiv : European Journal of Physiology - April 29, 2021 Category: Physiology Authors: Jasmin Ballout Martin Diener Source Type: research
