Recombinant Nipah F Proteins and Their Use

Nipah virus is an emerging pathogenic paramyxovirus responsible for sporadic and isolated outbreaks of severe respiratory and neurologic disease in Southern Asia. As a zoonotic virus, disease can manifest in both animals and human with indigenous fruit bats acting as natural reservoirs of the virus. The effects of viral infection vary from acute respiratory distress to fatal encephalitis. There are currently no approved therapeutics or vaccines against the virus, and growing concerns that this highly pathogenic infection has the potential to cause larger epidemics capable of inflicting significant mortality burden.Like the RSV fusion (F) glycoprotein, the Nipah fusion glycoprotein is a target of neutralizing antibodies that mediate protection against infection. Previous studies of prefusion-stabilized F glycoproteins from pneumoviruses and other paramyxoviruses (e.g. RSV and PIVs) have shown they elicit higher titers of neutralizing antibodies in both animals and humans than post-fusion F proteins.Researchers at the Vaccine Research Center (VRC) of the National Institute of Allergy and Infectious Diseases (NIAID) designed disulfide, cavity-filling and other mutations that stabilize the Nipah F glycoprotein in the prefusion conformation and bind prefusion-specific antibodies. These mutations also increase protein expression yields up to 50-fold making the recombinant proteins easy to manufacture and amenable to the use of genetic immunization using nucleic acid or vector-based...
Source: NIH OTT Licensing Opportunities - Category: Research Authors: Source Type: research

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Vincent speaks with Félix Rey about his career and his work on solving structures of a variety of viruses and the insights learned about viral membrane fusion and antibody-mediated neutralization. Click arrow to play Download TWiV 577 (46 MB .mp3, 75 min) Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Show notes at microbe.tv/twiv
Source: virology blog - Category: Virology Authors: Tags: This Week in Virology antibody neutralization cryo-EM dengue virus flavivirus fusion loop glycoprotein Semliki Forest virus tick borne encephalitis virus viral viral membrane fusion viruses X-ray crystallography zika virus Source Type: blogs
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Source: Colloids and Surfaces B: Biointerfaces - Category: Biochemistry Source Type: research
Fight Aging! publishes news and commentary relevant to the goal of ending all age-related disease, to be achieved by bringing the mechanisms of aging under the control of modern medicine. This weekly newsletter is sent to thousands of interested subscribers. To subscribe or unsubscribe from the newsletter, please visit: https://www.fightaging.org/newsletter/ Longevity Industry Consulting Services Reason, the founder of Fight Aging! and Repair Biotechnologies, offers strategic consulting services to investors, entrepreneurs, and others interested in the longevity industry and its complexities. To find out m...
Source: Fight Aging! - Category: Research Authors: Tags: Newsletters Source Type: blogs
Tailor Cut Produce of New Jersey, is recalling its Fruit luau, cut honeydew, cut cantaloupe and cut pineapple products because they have the potential to be contaminated with Salmonella, an organism which can cause serious and sometimes fatal infections in young children, frail or elderly people.
Source: FDA Food Safety - Category: Food Science Authors: Source Type: alerts
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Source: Johnson and Johnson - Category: Pharmaceuticals Source Type: news
Vincent speaks with Félix Rey about his career and his work on solving structures of a variety of viruses and the insights learned about viral membrane fusion and antibody-mediated neutralization. Subscribe (free): iTunes, Google Podcasts, RSS, email Become a patron of TWiV! Links for this episode Unité de Virologie Structurale Tick-borne encephalitis glycoprotein structure (Nature) HCV RNA polymerase structure (PNAS) Flavivirus structural heterogeneity (Curr Op Viral) Antibody responses to flaviviruses (EMBO Rep) Covalently linked dengue envelope dimers (Nat Comm) Timestamp...
Source: This Week in Virology - MP3 Edition - Category: Virology Authors: Source Type: podcasts
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Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
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Source: Ticks and Tick borne Diseases - Category: Zoology Source Type: research
Authors: Babaer D, Zheng M, Ivy MT, Zent R, Tiriveedhi V Abstract Previous phase I DNA-vaccine based clinical trials using Mammaglobin-A (Mam-A), a human breast tumor associated antigen (TAA), demonstrated that this agent was safe and efficient at treating patients with stage IV breast cancer. The long-term success of cancer vaccines is limited by the diminished expression of human leukocyte antigen (HLA) class I molecules in the tumor microenvironment. The current study assessed the impact of various selenocompounds on the expression of HLA class I molecules in THP-1 cells, an apparent proficient antigen that pres...
Source: Oncology Letters - Category: Cancer & Oncology Tags: Oncol Lett Source Type: research
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