Using a Proximity Ligation Assay to Diagnose Lymphoma Subsets and Predict Patient Responses to Targeted Therapies
Diffuse large B cell lymphoma (DLBCL) is a common and aggressive malignancy that accounts for 38-50% of lymphomas each year. There are two distinct subtypes of DLBCL: germinal center B cell-like (GCB) and activated B cell-like (ABC). Patients with ABC DLBCL have an inferior overall survival following multi-agent chemotherapy and respond differently to treatment compared to GCB DLBCL. In particular, the B cell receptor (BCR) signaling inhibitor, Ibrutinib, produced a response in 37% of DLBCL tumors with an ABC gene expression profile versus a 5% response in those tumors with a GBC gene expression profile. However, ABC cases...
Source: NIH OTT Licensing Opportunities - June 10, 2019 Category: Research Authors: ajoyprabhu3 Source Type: research

One-step random amplification method to detect extremely low input nucleic acids for virome, microbiome, and metagenomics in clinical and biological specimens
Clinical and biological specimens often contain microbial nucleic acid in extremely low quantities, presenting a significant challenge for the detection of viral and bacterial pathogens. This also prevents direct sequencing of non-culturable samples using next-generation sequencing (NGS). Currently, NGS library preparation on most platforms requires 0.1 ng to 10 µg of DNA or cDNA, while microbial or viral nucleic acids in clinically relevant specimens, such as blood, serum, respiratory secretions, cerebral spinal fluid, and stool, often contain less than 0.1 ng.CDC developed a rapid random amplification method capabl...
Source: NIH OTT Licensing Opportunities - May 28, 2019 Category: Research Authors: ajoyprabhu3 Source Type: research

Treatment of Oculocutaneous/Ocular Albinism and for Increasing Pigmentation
Albinism (also called achromia, achromasia, or achromatosis) is a congenital disorder characterized by the complete or partial absence of pigment in the skin, hair and eyes due to absence or defect in any one of a number of proteins involved in the production of melanin.   Certain forms of albinism are known to be due to mutations in tyrosine metabolism.  In oculocutaneous albinism (OCA), pigment is lacking in the eyes, skin and hair.  In ocular albinism, only the eyes lack pigment.  Patients with albinism experience varying degrees of vision loss associated wit h foveal hypoplasia, nystagmus, photophob...
Source: NIH OTT Licensing Opportunities - May 17, 2019 Category: Research Authors: ajoyprabhu3 Source Type: research

Metformin for the Treatment of Age-related Retinal Degeneration
Retinal Degenerations (RD) are the leading cause of blindness in the United States. The degeneration of the Retinal Pigment Epithelium (RPE) is associated with various types of RD such as Stargardt ’s disease, retinitis pigmentosa, choroideremia, Late-Onset Retinal Degeneration (L-ORD), and Age-related Macular Degeneration (AMD). The RPE as a layer of cells in the back of the eye. Therefore, it is essential to maintain the health and integrity of retinal photoreceptors. RPE dysfunction and d egeneration leads to photoreceptor cell death and vision loss, a common factor among several forms of RD.To resolve these chall...
Source: NIH OTT Licensing Opportunities - May 15, 2019 Category: Research Authors: ajoyprabhu3 Source Type: research

A Most Efficient and Convergent Principal Component Analysis (PCA) Method for Big Data
Big data usually means big sample size with many outliers, in which traditional scalable L2-norm principal component analysis (L2-PCA) will fail. Current existing L1-norm PCA (L1-PCA) methods can improve robustness over outliers, however, its scalability is usually limited in either sample size or dimension size.   The inventor proposes an online flipping method to solve L1-PCA challenges, which is not only convergent asymptotically (or with big data), but also achieves most efficiency in the sense each sample is visited only once to extract one principal component (PC). The proposed PCA also has certain r obustn...
Source: NIH OTT Licensing Opportunities - May 15, 2019 Category: Research Authors: ajoyprabhu3 Source Type: research

Recombinant Virus Vectors for the Treatment of Glycogen Storage Disease type Ib (GSD-Ib)
Glycogen storage disease type Ib (GSD-Ib) is an autosomal recessive disorder caused by deficiencies in glucose-6-phosphate transporter (G6PT), a ubiquitously expressed endoplasmic reticulum (ER) protein that translocates G6P from the cytoplasm into the ER lumen.   Inside the ER, G6P is hydrolyzed to glucose and phosphate by either the liver/kidney/intestine-restricted glucose-6-phosphatase-α (G6Pase-α or G6PC) or the ubiquitously expressed G6Pase-β.  G6PT and G6Pase are functionally co-dependent and form the G6PT/G6Pase complexes. The G6PT/G6Pase-α co mplex maintains interprandial blood glu...
Source: NIH OTT Licensing Opportunities - May 15, 2019 Category: Research Authors: ajoyprabhu3 Source Type: research

High Affinity Cross Species Single Domain Antibodies Targeting Mesothelin
Mesothelin is a cell surface protein that is an excellent target for immunotherapy because of its limited expression on normal tissues and its high expression on many cancers, including mesothelioma, cholangiocarcinoma, pancreatic, ovarian, lung, stomach, bile duct, and triple-negative breast cancer.Researchers at the National Cancer Institute ’s (NCI)Laboratory of Molecular Biology have isolated two anti-mesothelin single domain antibodies (also known as nanobodies), A101 and G8. These antibodies have been isolated from newly developed camel single domain (VHH) libraries by phage display and have been used to shown ...
Source: NIH OTT Licensing Opportunities - May 15, 2019 Category: Research Authors: ajoyprabhu3 Source Type: research

Monoclonal Antibodies and Immunoconjugates Directed to the Non-ShedPortion ( “Stalk”) of Mesothelin are Excellent Candidates for Developing Therapeutic Agents
Human mesothelin is overexpressed by various cancers such as synovial sarcoma, mesothelioma, and ovarian, lung, esophageal, and gastric cancers. This selective expression on certain cancers suggests that mesothelin is an excellent target for anticancer therapeutics. However, a large fragment ( “the shed portion”) of mesothelin is constantly shed from cells, and all current anti-mesothelin antibodies bind to the shed portion. As a result, the therapeutic efficacy of these antibodies has been low because they are unable to exert their therapeutic effect on the cell before their binding region is shed.Fortunately,...
Source: NIH OTT Licensing Opportunities - April 26, 2019 Category: Research Authors: ajoyprabhu3 Source Type: research

High Affinity Monoclonal Antibodies Targeting Glypican-1
Pancreatic cancer is the fourth most common cause of death from cancer in the U.S. The overall 5-year survival rate for this disease is 8.5%. Glypican-1 (GPC1), a cell surface heparan sulfate proteoglycan protein that is overexpressed in pancreatic cancer. Due to this preferential expression, GPC1 represents a potential candidate for targeted therapy for patients with pancreatic cancer and other GPC1 expressing cancers such as prostate cancer.Researchers at the National Cancer Institute ’s (NCI)Laboratory of Molecular Biology have developed and isolated two new antibodies that target GPC1 (HM2 and D4). These new anti...
Source: NIH OTT Licensing Opportunities - April 26, 2019 Category: Research Authors: ajoyprabhu3 Source Type: research

Nanoparticle-hydrogel Composite for Nucleic Acid Molecule Delivery
Mesothelioma is an aggressive cancer covering anatomic surfaces (e.g. lining of the lungs, heart, abdomen, etc.) that resists multi-modality therapies. Regional recurrence of mesothelioma from residual tumor cells prevents long-term benefits after surgical resection. Furthermore, there is no clinical consensus on intracavitary adjuvants that are effective in extending the tumor reduction effect of surgery. Researchers at the National Cancer Institute (NCI) developed a new technology that provides a localized, regional therapeutic platform to transport cancer-specific microRNA to a cancerous surface.  This nanopar...
Source: NIH OTT Licensing Opportunities - April 25, 2019 Category: Research Authors: ajoyprabhu3 Source Type: research

Chimeric Antigen Receptor (CAR) that Targets Chemokine Receptor CCR4 and its Use in Treating Cancer
The chemokine receptor CCR4 is a seven-transmembrane G protein-coupled cell surface receptor molecule with selective expression on cells of the hematopoietic system. In adult T cell leukemia (ATL), the cell-surface expression of CCR4 on leukemic cells has been found to be nearly universal. Therefore, a CCR4-directed CAR-cell may provide an effective therapeutic against ATL.Researchers at the National Cancer Institute, Lymphoid Malignancies Branch developed a lentivirus-derived chimeric antigen receptor (CAR) against the CCR4 molecule. The CAR can be directed to either genetically modified autologous or to allogeneic T- or ...
Source: NIH OTT Licensing Opportunities - April 23, 2019 Category: Research Authors: ajoyprabhu3 Source Type: research

Monitoring Public Water Supply for a Variety of Pathogens
The simultaneous concentration and recovery of microbes in drinking water is important for responding to potential water-related events such as pathogen contamination or bioterrorism and could be a cost-effective technique for routine monitoring of drinking water quality. Scientists at the CDC have combined two techniques, ultrafiltration (UF) and insulator-based dielectrophoresis (iDEP) in series, to achieve significant concentration of microbes and pathogens for analysis. UF can concentrate a water sample ≥200X, depending on turbidity; if a secondary concentration step is applied, then a ≥25,000X can be achieved. R...
Source: NIH OTT Licensing Opportunities - April 12, 2019 Category: Research Authors: ajoyprabhu3 Source Type: research

Monoclonal Antibody to Detect the Antiretroviral Drug Emtricitabine – for HIV Drug Adherence Monitoring
The U.S. Food and Drug Administration and World Health Organization (WHO) approved the antiretroviral drug emtricitabine (FTC)/ tenofovir disoproxil fumurate (TDF) combination for HIV pre-exposure prophylaxis (PrEP) in high risk populations. Efficacy of PrEP depends strongly on adherence to taking the FTC/TDF pill daily. In the US, the Centers for Disease Control and Prevention (CDC) estimates that 1.2 million Americans will benefit from PrEP. FTC is also a key component of antiretroviral therapy (ART) regimens of HIV-infected persons and significantly associated with adherence. According to reports from the WHO, 21.7 mill...
Source: NIH OTT Licensing Opportunities - April 11, 2019 Category: Research Authors: ajoyprabhu3 Source Type: research

Real-Time RT-PCR Detection of Scrub Typhus Total Nucleic Acid Assay with High Sensitivity and Specificity
Scrub typhus is a bacterial disease caused by Orientia tsutsugamushi (O. tsutsugamushi or Ots) that is spread to people through bites of infected chiggers (larval mites). The most common symptoms can include fever, headache, body aches, and sometimes rash. Severe illness can lead to organ failure and bleeding which can be fatal if left untreated. Most cases of scrub typhus occur in Asia Pacific countries, however, recent reports document establishment in the Arabian Peninsula, Chile, and possibly Kenya. Anyone living in or traveling to areas where scrub typhus is found could get infected if they come in contact with infect...
Source: NIH OTT Licensing Opportunities - April 3, 2019 Category: Research Authors: ajoyprabhu3 Source Type: research

Encapsulated Streptococcus Compositions and Methods for Pneumococcal Vaccine, Probiotic, and Diagnostic Assay Development
Streptococcus pneumoniae (S. pneumoniae) em>bacteria, or pneumococcus, can cause many types of illnesses. These range from ear and sinus infections to life-threatening conditions such as pneumonia, bloodstream infections, and meningitis. Pneumococci are surrounded by a polysaccharide capsule, which is thought to help it evade the immune system. Presently, over 90 known serotypes ofS. pneumoniae em> have been identified, of which only a minority produce the majority of pneumococcal infections; a serotype is defined by a unique pneumococcal capsule structure. Currently available vaccines contain antigens made from caps...
Source: NIH OTT Licensing Opportunities - February 22, 2019 Category: Research Authors: ajoyprabhu3 Source Type: research

Monoclonal Antibodies that Bind Zika Virus Envelope Protein for Zika Diagnostics and Research
Zika virus infection during pregnancy can cause microcephaly and other severe birth defects. The CDC Zika MAC-ELISA (IgM antibody capture enzyme-linked immunosorbent assay) currently used for diagnosis detects antibodies produced to fight a Zika virus infection. However, reactivity of flavivirus antibodies (from exposure to other mosquito-borne infections such as dengue or West Nile virus) can complicate the interpretation of these results.CDC and partner researchers have developed six monoclonal antibodies (mAbs) from hybridoma technology with high sensitivity to the Zika virus (ZIKV) pre-membrane/envelope (ENV) protein a...
Source: NIH OTT Licensing Opportunities - February 22, 2019 Category: Research Authors: ajoyprabhu3 Source Type: research

Potential Treatment for sickle-cell disease and thalassemia
The technology addresses treatment options for diseases such as sickle-cell and thalassemia. Traditionally, such beta-globinopathies are treated through bone marrow transplantation. However, this method is limited due to high treatment costs and finding a matched-donor. This relies on increasing fetal hemoglobin to potentially cure the disease. NIH inventors have identified a protein called Rio-Kinase 3 (RIOK3), that inhibits the production of fetal hemoglobin. Their work shows that inhibiting RIOK3 increases the production of fetal hemoglobin. Thus, RIOK3 is a promising novel therapeutic target to increase fetal hemoglobi...
Source: NIH OTT Licensing Opportunities - January 17, 2019 Category: Research Authors: ajoyprabhu3 Source Type: research

Hydrocarbon Stapled Peptides that Inhibit the Linear Ubiquitin Chain Assembly Complex (LUBAC) for the Therapy of the Activated B Cell-like (ABC) Subtype of Diffuse Large B Bell Lymphoma (A Type of Non-Hodgkin ’s Lymphoma)
Diffuse large B-cell lymphoma (DLBCL) is the most common type of non-Hodgkin ’s lymphoma and consists of three subtypes: activated B-cell (ABC), germinal center B-cell (GBC), and primary mediastinal B-cell (PMB). Despite advances in the front-line therapy for DLBCL, approximately one-third of patients will relapse. Substantially worse outcomes have been reported for patien ts diagnosed with ABC DLBCL and treated with standard chemoimmunotherapy, suggesting the need for novel strategies that improve treatment outcomes.ABC DLBCL cell survival depends largely upon NF- κB signaling being constitutively active. The ...
Source: NIH OTT Licensing Opportunities - December 20, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Hydrocarbon Stapled Peptides that Inhibit the Linear Ubiquitin Chain Assembly Complex (LUBAC) for the Therapy of the Activated B Cell-like (ABC) Subtype of Diffuse Large B Bell Lymphoma (A Type of Non-Hodgkin ’s Lymphoma)
Diffuse large B-cell lymphoma (DLBCL) is the most common type of non-Hodgkin ’s lymphoma and consists of three subtypes: activated B-cell (ABC), germinal center B-cell (GBC), and primary mediastinal B-cell (PMB). Despite advances in the front-line therapy for DLBCL, approximately one-third of patients will relapse. Substantially worse outcomes have been reported for patien ts diagnosed with ABC DLBCL and treated with standard chemoimmunotherapy, suggesting the need for novel strategies that improve treatment outcomes.ABC DLBCL cell survival depends largely upon NF- κB signaling being constitutively active. The ...
Source: NIH OTT Licensing Opportunities - December 14, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

siRNA Delivery Using Hexameric Tetrahedral RNA Nanostructures for Gene Silencing
RNA interference (RNAi) is a biological response to double-stranded RNA that regulates expression of protein-coding genes and is a natural mechanism for gene silencing. Delivery of short, interfering RNA (siRNA) leads to RNAi of the targeted genes.  Researchers at the National Cancer Institute (NCI), in collaboration with researchers at the University of California, Santa Barbara (UCSB), developed a tetrahedral-shaped RNA nanoparticle for the delivery of siRNA to activate RNAi. The tetrahedral RNA nanoparticle is comprised of four RNA nanorings as the “faces” of the tetrahedral scaffold. The tetrahedr...
Source: NIH OTT Licensing Opportunities - December 14, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Methods of Producing Thymic Emigrants from Induced Pluripotent Stem Cells
Hematopoietic and pluripotent stem cells can be differentiated into T cells with potential clinical utility. Current approaches for in vitro T cell production rely on Notch signaling and artificial mimicry of thymic selection. However, these approaches result in unconventional or phenotypically aberrant T cells; which may lead to unpredictable behavior in clinical use. Thus, there exists a need for improved methods of generating conventional T cells in vitro from stem cells.  Researchers at the National Cancer Institute (NCI) have developed a novel method for the in vitro differentiation of induced pluripotent stem c...
Source: NIH OTT Licensing Opportunities - December 14, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Melanoma-Associated Antigen-A (MAGE-A3/A6) -Specific Monoclonal Antibody
A T cell receptor (TCR) recognizing melanoma-associated antigen-A (MAGE-A3/A6) is currently used in clinical trials involving adoptive transfer of TCR-transduced T cells for patients with metastatic cancer. To be eligible for this TCR immunotherapy, tumor specimens from patients must show homogeneous expression of MAGE-A3 and/or MAGE-A6. Currently-available antibodies recognize additional members of the MAGE-A family and/or are cross-reactive because they were raised by epitopes shared among the MAGE-A family members.MAGE-A3 and MAGE-A6 are members of the MAGE-A family of twelve homologous proteins. The MAGE-A proteins are...
Source: NIH OTT Licensing Opportunities - December 14, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Recombinant HIV-1 Envelope Proteins and Their Use
An effective human immunodeficiency virus type 1 (HIV-1) vaccine has long been sought to contend with the Acquired Immunodeficiency Syndrome (AIDS) pandemic.One approach researchers have taken to elicit broadly neutralizing antibodies against HIV-1 is to stabilize the structurally flexible HIV-1 envelope (Env) trimer. Researchers stabilized the Env trimer in a conformation that displays predominantly broadly neutralizing epitopes and few non-neutralizing epitopes. Currently, BG505 DS-SOSIP is a leading vaccine candidate with the desired conformation and antigenicity.Ideally, to be useful as a vaccine, such a conformational...
Source: NIH OTT Licensing Opportunities - November 27, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Optimized Variants of the Broadly Neutralizing HIV-1 gp41 Antibody, 10E8
Scientists at the National Institute of Allergy and Infectious Diseases (NIAID) recently discovered a human neutralizing antibody, 10E8, that binds to the GP41 protein of HIV-1 and prevents infection by HIV-1. 10E8 potently neutralizes up to 98% of genetically diverse HIV-1 strains.By engineering the 10E8 antibody, NIAID scientists have improved the properties of 10E8 that affect manufacturability, such as solubility, while preserving its neutralizing breadth and potency.10E8 variants are useful for passive protection from infection, as therapeutics, and as a tool for vaccine development.This technology is available for li...
Source: NIH OTT Licensing Opportunities - November 19, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Large-scale Purification of GMP Chimeric Antibodies
This technology is a method for purifying a biologic composition, comprising  diafiltering the biologic composition into a composition comprising phosphate buffered saline (PBS) to obtain a purified composition. The method is particularly useful for removing one or more impurities from the biologic composition, such as bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methane (B is-tris).The technology is directed to large scale manufacturing of Chimeric 14.18 (Ch14.18) monoclonal antibodies. Ch14.18 is an anti-GD2 monoclonal antibody and has been described in Gillies et al., Journal of Immunological Methods 125:1...
Source: NIH OTT Licensing Opportunities - November 15, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Novel Small Molecules that Inhibit Hepatitis B Virus Replication by Targeting Packaging of Pre-genomic RNA
Hepatitis B virus (HBV) is the smallest animal-infecting DNA virus with a compact 3.2 kDa genome that encodes seven proteins contained in four overlapped genes. Its small genome size and limited coding capacity make finding effective anti-HBV therapies difficult. Current treatment strategies for chronic HBV include liver transplantation or broad-spectrum nucleos(t)ide analogs including Lamivudine and Tenofovir, which are used to achieve viral suppression. Unfortunately, long-term administration of these nucleos(t)ide analogs is costly and can lead to the development of resistance mutations. While vaccines exist for HBV, th...
Source: NIH OTT Licensing Opportunities - November 14, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Novel Small Molecule Antagonists Targeting Metastasis-Associated Lung Adenocarcinoma Transcript 1 (MALAT1) Long Non-coding RNA (lncRNA) as Anticancer Agents
Human metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a long non-coding RNA (lncRNA) overexpressed in multiple human cancers. There is a strong correlation between MALAT1 levels and increased risk of various malignancies including metastasis. Antisense oligonucleotide inhibition of MALAT1 expression levels has shown promising anticancer effects in vivo. Additionally, depletion of MALAT1 is not lethal to normal cell growth, further supporting MALAT1 as a promising therapeutic target for cancer therapy.The enhancement of oncogenic processes by MALAT1 in cancers has been localized to its 3 ’-end seque...
Source: NIH OTT Licensing Opportunities - November 14, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Non-Invasive In Vivo MRI Method to Image Salient Features of Axons and Nerves
The invention from Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD) provides a non-invasive, painless means for measuring microanatomical features of Nerve and Axon Diameter Distribution (ADD) to image the Central and Peripheral Nervous Systems (CNS and PNS). ADD is altered in abnormal development (e.g., autism), in neurodegenerative processes (e.g., aging, alcoholism, Alzheimer ’s disease) and diseases such as ALS (Lou Gehrig’s disease). U.S. patent 7,643,863, related to this invention, consists of a novel diffusion Magnetic Resonance Imaging (MRI) experiment and mod...
Source: NIH OTT Licensing Opportunities - November 14, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Engineered Biological Pacemakers
The National Institute on Aging's (NIA) Cellular Biophysics Section is seeking statements of capability or interest from parties interested in collaborative research to further develop, evaluate, or commercialize biological pacemakers.A common symptom of many heart diseases is an abnormal heart rhythm or arrhythmia.  While effectively improving the lives of many patients, implantable pacemakers have significant limitations such as limited power sources, risk of infections, potential for interference from other devices, and absence of autonomic rate modulation.The technology deveoped by the NIA, consists of biological ...
Source: NIH OTT Licensing Opportunities - November 8, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Bicistronic Chimeric Antigen Receptor (CAR) Constructs Targeting CD19 and CD20
CD19 and CD20 are promising targets for the treatment of B-Cell malignancies.   Unfortunately, some clinical studies have shown that there is a loss of CD19 or CD20 expression in various cases of lymphomas and leukemias, particularly after treatment with an agent that targets CD19 (e.g., anti-CD19 CAR-T). However, studies have shown that expression of one protein is retained when the other is lost. This suggests that a therapeutic with the ability to simultaneously target both CD19 and CD20 could represent a solution to the drawbacks of current therapies. Researchers at the National Cancer Institute (NCI) have de...
Source: NIH OTT Licensing Opportunities - November 8, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Bioluminescent Bladder Cancer Cell Line for Tracking Cancer Progression
Bladder cancer is the fifth most common cancer in the United States and one of the costliest cancers to treat. Compared to other cancer types, bladder cancer has been understudied, and there is a need for informative mouse bladder cancer models that resemble the clinical situation and allow for evaluation of chemotherapeutic or immunotherapeutic agents. The orthotopic murine bladder cancer model MB49 resembles non-muscle invasive, nonmetastatic urothelial carcinomas and provides an opportunity to study the anti-tumor effects of immune cell checkpoint inhibitors. Moreover, successful tumor cell implantation of MB49 cells fa...
Source: NIH OTT Licensing Opportunities - November 8, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Universal Influenza Virus Probes for Enrichment of Influenza Viral Sequences
This technology is a set of influenza virus enrichment probes developed to increase the sensitivity of sequence-based, universal detection of all influenza viruses. This universal influenza enrichment probe set contains a unique set of 46,953 biotin-labeled, RNA probes, each 120 base-pairs long, that can be used to enrich for any influenza sequences without prior knowledge of type or subtype. This probe set can capture and enrich influenza viral sequences selectively and effectively in a variety of samples, such as clinical samples with degraded nucleotides or samples containing very low amounts of influenza virus, thus ma...
Source: NIH OTT Licensing Opportunities - November 8, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Methods for Dagnosing and Treating Mycobacterium tuberculosis (Mtb) Infection through Detection of CD153 Expression Level.
Mycobacterium tuberculosis (Mtb) infection continues to be the leading cause of death due to a single infectious agent and poses significant global health challenges. Past research has shown that CD4 T cells are essential for resistance to Mtb infection, and for decades it has been thought that IFN( γ) production is the primary mechanism of CD4 T cell-mediated protection.NIAID researchers have discovered that the expression of TNF superfamily molecule CD153 (TNSF8) is required for control of the pulmonary Mtb infection by CD4 T cells. The results have shown that, in Mtb infected mice, CD153 expression is highest on A...
Source: NIH OTT Licensing Opportunities - November 6, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Antibodies and Methods for the Diagnosis and Treatment of Epstein-Barr Virus Infection
According to the World Health Organization, over 90% of the worldwide population is infected with Epstein-Barr virus by adulthood. In most cases, the disease accompanying initial infection is subclinical though some individuals who are infected as adolescents or adults do experience infectious mononucleosis. However, once infected, individuals carry latent EBV for their remaining lifespan. In such individuals, immune suppression can result in reactivation of the EBV and consequently, EBV-associated lymphoproliferative disease. Currently, there is no prophylactic to prevent primary EBV infection and additional therapeutics ...
Source: NIH OTT Licensing Opportunities - November 5, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Middle East Respiratory Syndrome Coronavirus Antibodies
Middle East Respiratory Syndrome coronavirus (MERS-CoV) causes a highly lethal pulmonary infection with ~35% mortality. Currently there are no prophylactic measures or effective therapies. Inventors at the Vaccine Research Center of the National Institute of Allergy and Infectious Diseases have identified and developed neutralizing monoclonal antibodies (nMAbs) against the MERS-CoV. This invention describes antibodies that target the Spike (S) glycoprotein on the coronavirus surface, which mediates viral entry into host cells. These novel antibodies target different regions of the S protein, and when administered in combin...
Source: NIH OTT Licensing Opportunities - November 5, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Fusion Glycoprotein Vaccine for Human Metapneumovirus
Human metapneumovirus (hMPV), a negative, single-stranded RNA virus, accounts for approximately 5-15% of infant respiratory tract infections and poses a severe risk of disease and hospitalization in both the elderly and the immunocompromised. Investigators at the Vaccine Research Center (VRC) of the National Institute of Allergy and Infectious Diseases (NIAID) have generated an hMPV fusion glycoprotein ( “F protein”) stabilized in a prefusion conformation.Stabilizing this prefusion conformation of the F protein reveals an immunodominant site which makes it an ideal vaccine immunogen. The prefusion stabilized F ...
Source: NIH OTT Licensing Opportunities - October 29, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Recombinant Respiratory Syncytial Virus Challenge Strain
RSV is the most important viral agent of severe respiratory tract disease worldwide, especially in infants and young children, and it also causes severe disease in the elderly and in immunocompromised individuals. There are no licensed vaccines or antivirals suitable for routine use.This invention relates to a reverse genetics system and cDNA-derived virus for a contemporary wild-type clinical isolate of RSV of antigenic subgroup A, termed RSV strain A/Maryland/001/11, that was isolated in 2011 from an adult with respiratory illness. The genomic sequence was determined. A reverse genetics system was created encoding a reco...
Source: NIH OTT Licensing Opportunities - October 25, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Use of Rostafuroxin to Inhibit Viral Infection
Acute respiratory infections during early childhood constitute a major human health burden. Human respiratory syncytial virus (RSV) is the most common and important viral cause of severe acute pediatric respiratory infections worldwide. Mortality due to RSV in the post-neonatal (28 days to 1 year old) population is second only to malaria. It is estimated that RSV causes 34 million lower respiratory tract infections, 4 million hospitalizations, and 66,000-199,000 deaths every year in children less than 5 years of age. Most mortality occurs in the developing world where clinical care is less accessible. Mortality is low in t...
Source: NIH OTT Licensing Opportunities - October 25, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Polarimetric Accessory for Colposcope
In medical diagnostic procedures for examining the cervix and the tissues of the vagina and vulva, long working-distance (-30 cm) lighted binocular microscopes (colposcope) that provide up to 25x optical magnification are used to create an illuminated magnified view. Speculum dilations can give rise to specular reflections from the tissue surface, causing physicians to overlook possible abnormalities – thus decreasing the quality of a colposcopy. Researchers at the National Institute of Child Health and Human Development (NICHD) developed a polarimetric accessory that overcomes this limitation and enhances the v...
Source: NIH OTT Licensing Opportunities - October 23, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Self-Assembling Nanoparticles Composed of Transmembrane Peptides and Their Application for Specific Intra-Tumor Delivery of Anti-Cancer Drugs
Peptides corresponding to transmembrane domains of a number of integral proteins were discovered to spontaneously self-assemble in aqueous solutions into stable and remarkably uniform nanoparticles.   Researchers at the NCI’s Cancer and Inflammation Program have developed fully synthetic, peptide-based, virus-like nanoparticles capable of delivering cytotoxic, radioactive, and imaging agents. Structure and function of tumor-target self-assembling particles: The current invention discloses peptide based fusogenic virus-like nanoparticles that can be targeted to tumors.   The nanoparticles have a diamet...
Source: NIH OTT Licensing Opportunities - October 23, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Hybridoma cell lines producing antibodies to RSV NS1
This technology provides a new set of hybridoma cell lines each expressing a single monoclonal antibody against human respiratory syncytial virus (RSV) nonstructural protein 1 (NS1). These antibodies have variously been shown to detect NS1 protein in an enzyme-linked immunosorbent assay (ELISA), Western blot assay, immunofluorescence microscopy of paraformaldehyde-fixed cells, and flow cytometry. The various antibodies can vary in their efficiency in each of these assays. This technology provides a unique set of qualified monoclonal antibodies against RSV NS1 protein which currently do not exist. These antibodies and cell ...
Source: NIH OTT Licensing Opportunities - October 18, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Recombinant HIV-1 Envelope Protein for Vaccine Use
In pursuit of an effective vaccine to end the global HIV-1/AIDS pandemic, researchers at the Vaccine Research Center ( “VRC”) continue to study the structure of HIV-1. Recently, these researchers have determined the three-dimensional structure of the HIV-1 Envelope trimeric ectodomain (“Env”), comprised of three gp120 and three gp41 subunits, in its prefusion, mature, closed conformation.The researchers hypothesize that immunization with the prefusion, closed HIV-1 Env protein will elicit a neutralizing immune response. The VRC researchers engineered a portion of the HIV-1 Env trimer to stabilize it...
Source: NIH OTT Licensing Opportunities - October 18, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

HIV-1 Env Fusion Peptide Immunogens and Their Use
Millions of people are infected with HIV-1 worldwide, and 2.5 to 3 million new infections have been estimated to occur yearly. Although effective antiretroviral therapies are available, millions succumb to AIDS every year, especially in Sub-Saharan Africa, underscoring the need to develop measures to prevent the spread of this disease.HIV-1 is an enveloped virus, which hides from humoral recognition behind a wide array of protective mechanisms. During infection, the major envelope protein of HIV-1 is cleaved by host cell proteases into two smaller versions (gp120 and gp41). Together gp120 and gp41 make up the HIV-1 Env spi...
Source: NIH OTT Licensing Opportunities - October 17, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Self-assembling Insect Ferritin Nanoparticles for Display of Co-assembled Trimeric Antigens
Antigens on the surface of virus particles are displayed in a regular, repetitive pattern which facilitates B cell activation. Presenting trimeric antigens on engineered particles that mimic the geometric patterns observed for native viral proteins can lead to an improved host antibody response.Self-assembling globular ferritin nanoparticles have previously been used to display multiple copies of a co-assembled trimeric antigen to the immune system. However, prior ferritin nanoparticle technologies only permit a random co-assembly of diverse trimeric antigens, and therefore cannot guarantee the pattern and ratio of diverse...
Source: NIH OTT Licensing Opportunities - October 17, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

HIV-1 Env Fusion Peptide Immunogens and Their Use
Millions of people are infected with HIV-1 worldwide, and 2.5 to 3 million new infections have been estimated to occur yearly. Although effective antiretroviral therapies are available, millions succumb to AIDS every year, especially in Sub-Saharan Africa, underscoring the need to develop measures to prevent the spread of this disease.HIV-1 is an enveloped virus, which hides from humoral recognition behind a wide array of protective mechanisms. During infection, the major envelope protein of HIV-1 is cleaved by host cell proteases into two smaller versions (gp120 and gp41). Together gp120 and gp41 make up the HIV-1 Env spi...
Source: NIH OTT Licensing Opportunities - October 17, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Stabilized Group 2 Influenza Hemagglutinin Stem Region Trimers and Uses Thereof
Researchers at the Vaccine Research Center of the National Institute of Allergy and Infectious Diseases (NIAID) have designed influenza vaccine candidates based on group 2 influenza hemagglutinin (HA) proteins. These group 2 HA proteins were engineered to remove the highly variable head region and stabilize the remaining stem region. The researchers then fused the engineered group 2 HA stabilized stem with a ferritin subunit. The resulting fusion protein can self-assemble into nanoparticles which display group 2 HA stem domain trimers on their surface.These immunogens elicit cross-reactive antibodies to group 2 influenza v...
Source: NIH OTT Licensing Opportunities - October 17, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Substitutions-Modified Prefusion RSV F Proteins and Their Use
The respiratory syncytial virus (RSV) fusion (F) glycoprotein is the primary target of neutralizing antibodies. The F glycoprotein exists in at least two conformations, a meta-stable prefusion state, and an extremely stable postfusion state. Both states share several epitopes targeted by neutralizing antibodies, but it has been demonstrated that the prefusion conformation of F contains at least one epitope not present in the postfusion conformation. Natural infection results in neutralizing antibodies that are primarily directed against the prefusion conformation of F, not its postfusion conformation. The instability of th...
Source: NIH OTT Licensing Opportunities - October 17, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Self-assembling Insect Ferritin Nanoparticles for Display of Co-assembled Trimeric Antigens
Antigens on the surface of virus particles are displayed in a regular, repetitive pattern which facilitates B cell activation. Presenting trimeric antigens on engineered particles that mimic the geometric patterns observed for native viral proteins can lead to an improved host antibody response.Self-assembling globular ferritin nanoparticles have previously been used to display multiple copies of a co-assembled trimeric antigen to the immune system. However, prior ferritin nanoparticle technologies only permit a random co-assembly of diverse trimeric antigens, and therefore cannot guarantee the pattern and ratio of diverse...
Source: NIH OTT Licensing Opportunities - October 17, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

High-throughput assay for detection of rabies neutralizing antibodies
According to 2010-2014 World Health Organization (WHO) research, dog-transmitted human rabies was present or suspected in 150 countries and territories worldwide. Domestic dogs were the most common reservoir of the rabies virus in these countries, and more than 99% of human deaths were caused by dog-transmitted rabies. Rabies is 100% preventable in dogs with appropriate administration of vaccines.Virus neutralization assays are essential for assessing vaccine efficacy. Current methods to detect rabies-specific neutralizing antibodies involve skilled personnel using a traditional 8-well slide and manually reading a microsco...
Source: NIH OTT Licensing Opportunities - October 17, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research

Simian T-Cell Lymphotropic Virus Strain Type 3 (STLV-3) Subtype D Variant, a Highly Divergent STLV-3, for Development of Diagnostics, Therapeutics, Vaccines and Research Tools
Simian T-cell lymphotropic viruses (STLV) are nonhuman primate retroviruses closely related to the human T-lymphotropic virus (HTLV). Types I, II, and III of HTLV have been found in humans and are believed to have originated from cross-species transmission of STLV from infected nonhuman primates. The HTLV viruses are known to cause leukemia, lymphoma, and neurological disorders.CDC researchers discovered a strain of simian T-cell lymphotropic virus type 3 known as STLV-3 subtype D variant. STLV-3 may be widespread in primates hunted in West-Central Africa, including the monkey Cercopithecus mona, which has a known geograph...
Source: NIH OTT Licensing Opportunities - October 17, 2018 Category: Research Authors: ajoyprabhu3 Source Type: research