89Zr-Oxine Complex for In Vivo PET Imaging of Labelled Cells and Associated Methods
This technology from the NCI Molecular Imaging Program relates to a Zirconium-89 (89Zr)-oxine complex for cell labeling, tracking of labeled cells by whole-body positron emission tomography/computed tomography (PET/CT) imaging, and associated methods. A long half-life of 89Zr (78.4 hours), high sensitivity of PET, and absence of background signal in the recipient enable tracking cells over a week using low levels of labeling radioactivity without causing cellular toxicity.The 89Zr-oxine complex is synthesized quickly by mixing components at room temperature and produces high yields. Cell labeling is achieved by a short, ro...
Source: NIH OTT Licensing Opportunities - June 5, 2021 Category: Research Authors: ott-admin Source Type: research
Human Synovial Sarcoma Cell Line A2243
Synovial sarcoma is a cancer affecting mesenchymal cells in connective tissues. This rare cancer is typically linked to genetic abnormalities or exposure to radiation. Metastatic growth throughout the body can occur primarily through blood circulation. More than 90% of synovial sarcomas show a characteristic t(X;18)(p11;q11) translocation involving the SYT and SSX genes. The resulting SYT-SSX abnormal fusion protein causes misregulation of downstream gene expression, leading to tumor formation.Researchers at the National Cancer Institute (NCI), Laboratory of Cellular and Molecular Biology (LCMB), have derived a cell line, ...
Source: NIH OTT Licensing Opportunities - May 25, 2021 Category: Research Authors: ott-admin Source Type: research
Hybridomas to Human Immunoglobulins for SARS-CoV-2 Diagnostics and Additional Indications
Immunoglobulins play a key role in the immune system. CDC has developed and tested hybridoma cell lines (monoclonal antibody (mAb) clones) for human IgG and other immunoglobulins. The mAbs generated from those hybridomas could be used as a reagent (second Ab) of anti-human immunoglobins in a diagnostic assay for SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2), the virus that causes COVID-19 (coronavirus disease 2019) and other assays that detect antigen specific antibodies from human sera.Collaborating international scientists evaluated the mAbs which showed reactivity and specificity for human IgG (11), the I...
Source: NIH OTT Licensing Opportunities - May 17, 2021 Category: Research Authors: ott-admin Source Type: research
Diagnostic Assay to Detect Group C Rotavirus in Humans and Animals —Monoclonal Antibody-based ELISA (Enzyme-linked Immunosorbent Assay)
Rotaviruses cause severe gastroenteritis in humans and animals globally. Currently, there are eight known serogroups (A-H) of rotaviruses. Group C rotavirus (GpC RV) causes sporadic cases and outbreaks of acute diarrhea in children and adults worldwide. GpC RV is also associated with diarrhea in swine. Currently, no simple and reliable diagnostic test exists for GpC RV, so disease prevalence remains unknown.CDC has developed a monoclonal antibody (mAb) against the GpC RV VP6 protein. Scientists used the mAb to develop an immunoassay with sensitivity and specificity to human and animal GpC rotavirus, but not with GpA rotavi...
Source: NIH OTT Licensing Opportunities - May 13, 2021 Category: Research Authors: ott-admin Source Type: research
Synergistic Use of Exo VII Inhibitors And Quinolone Antibiotics For Treating Bacterial Infection
Topoisomerase poisons, such as quinolone antibiotics, are widely used as anticancer drugs and antibiotics. Quinolone antibiotics act by trapping prokaryotic type IIA topoisomerases (DNA gyrase and TOPO IV), resulting in irreversible topoisomerase cleavage complexes. However, current U.S. Food and Drug Administration (FDA) guidance reserves the use of quinolones for the most serious bacterial infections due to their associated side effects and to limit the occurrence of drug-resistant bacterial strains. Resistance to available antibiotics in pathogenic bacteria is a global challenge as the number of drug-resistant strains i...
Source: NIH OTT Licensing Opportunities - May 12, 2021 Category: Research Authors: ott-admin Source Type: research
Gene Therapy Vector for the Treatment of Glycogen Storage Disease Type Ia (GSD-Ia)
GSD-Ia is an inherited disorder of metabolism associated with life-threatening hypoglycemia, hepatic malignancy, and renal failure caused by the deficiency of glucose-6-phosphatase-alpha (G6Pase-alpha or G6PC). Current therapy, which primarily consists of dietary modification, fails to prevent long-term complications in many patients, including growth failure, gout, pulmonary hypertension, renal dysfunction, osteoporosis, and hepatocellular adenomas (HCA). Gene therapy-based techniques, which directly address the underlying genetic deficiency driving the disorder, offer the prospect of long-term remission in patients with ...
Source: NIH OTT Licensing Opportunities - May 6, 2021 Category: Research Authors: ott-admin Source Type: research
New Heterocyclic Scaffold-Based Inhibitors of the Polo-Box Domain of Polo-like Kinase 1 for the Treatment of Cancer
Polo-like kinase 1 (Plk1), a member of the Polo-like kinase family, plays a critical role in regulating mitosis and cell cycle progression. Aberrant expression of Plk1 has been observed in a variety of human cancers, and it is known to be associated with tumorigenesis as well as poor prognosis in cancer patients. Unlike normal cells, some cancer cells are dependent on augmented Plk1 levels to remain viable and are killed when Plk1 function is attenuated. Although Plk1 has proven to be an attractive target in cancer treatment, currently available Plk1 inhibitors have shown limited efficacy with significant dose-limiting tox...
Source: NIH OTT Licensing Opportunities - May 5, 2021 Category: Research Authors: ott-admin Source Type: research
Air-bubble Cushioning Application to Improve Shock Absorption Performance of Construction and Industrial Helmets
In this study, adding an air-bubble cushioni ng liner increased the helmet ' s shock absorbing endurance limit by 145%.The current study represents the first to use air bubble cushioning in helmet suspension systems. Results demonstrate that adding an air bubble cushioning liner to a Type I construction helmet would substantially improve the helmet ’s shock absorption performance at high impact forces, providing better protection and passing more stringent test standards. Findings may help manufacturers improve helmet designs, thereby reducing the potential for work-related TBIs. Incorporating air bubble cushioning into ...
Source: NIH OTT Licensing Opportunities - May 5, 2021 Category: Research Authors: ott-admin Source Type: research
Improved Gene Therapy Vectors for the Treatment of Glycogen Storage Disease Type Ia (GSD-1a)
GSD-Ia is an inherited disorder of metabolism associated with life-threatening hypoglycemia, hepatic malignancy, and renal failure caused by the deficiency of glucose-6-phosphatase-alpha (G6Pase-alpha or G6PC). Current therapy, which primarily consists of dietary modification, fails to prevent long-term complications in many patients, including growth failure, gout, pulmonary hypertension, renal dysfunction, osteoporosis, and hepatocellular adenomas (HCA). Gene therapy-based techniques, which directly address the underlying genetic deficiency driving the disorder, offer the prospect of long-term remission in patients with ...
Source: NIH OTT Licensing Opportunities - April 30, 2021 Category: Research Authors: ott-admin Source Type: research
High-Throughput Generation of Induced Pluripotent Stem Cells Carrying Antigen-Specific T Cell Receptors from Tumor Infiltrated Lymphocytes
One form of adoptive T cell therapy (ACT) consists of harvesting tumor infiltrating lymphocytes (TIL), screening and isolating TIL which display tumor antigen-specific T-cell receptors (TCR), expanding the isolated T cells in vitro, and reinfusing them into the patient for treatment. While highly active in the treatment of certain cancers (e.g., melanoma), current methods used to produce cancer-reactive T cells require significant time and may not adequately identify the desired TCRs which bind cancer targets.Researchers at the National Cancer Institute (NCI) Surgery Branch have developed a method which allows for the iden...
Source: NIH OTT Licensing Opportunities - April 28, 2021 Category: Research Authors: ott-admin Source Type: research
Monoclonal Antibody that Detects a Subclass of Dog IgG —for Diagnostic and Research Applications
CDC and collaborating researchers have developed a new monoclonal antibody that recognizes canine IgG (likely IgG4 subclass). This anti-dog IgG reagent could be used to detect antibody reactions to a variety of antigens and has potential use in a wide variety of diagnostic or research applications.The team used the published method of Mazza et al. (1993) to isolate a canine IgG fraction (peak Z) and used that fraction to create a new monoclonal antibody. The new monoclonal antibody appears to specifically recognize a particular subclass of canine IgG (likely IgG4) with high reactivity and low background. Very few commercia...
Source: NIH OTT Licensing Opportunities - April 27, 2021 Category: Research Authors: ott-admin Source Type: research
Newcastle Disease Virus-Like Particle Displaying Prefusion Stabilized SARS-CoV-2 Spike and Its Use
SARS-CoV-2 has resulted in a global pandemic, sparking urgent vaccine development efforts. The trimeric SARS-CoV-2 spike stabilized in its prefusion conformation by the addition of 2 proline mutations ( “SARS-CoV-2 S2P”) is the antigenic basis of SARS-CoV-2 vaccines that are currently authorized for use in the United States.Researchers at the Vaccine Research Center (VRC) of the National Institute of Allergy and Infectious Diseases (NIAID) sought to optimize the presentation of SARS-CoV-2 S2P to the immune system with the goal of eliciting a strong and durable immune response. The researchers designed fusion proteins m...
Source: NIH OTT Licensing Opportunities - April 23, 2021 Category: Research Authors: ott-admin Source Type: research
Iodonium Analogs as Inhibitors of NADPH Oxidases and other Flavin Dehydrogenases and their Use for Treating Cancer
Diverse human cancers like colorectal, pancreatic, ovarian, melanoma, and pre-cancers express NADPH oxidases (NOX) at high levels. Reactive oxygen species (ROS) produced from metabolic reactions catalyzed by NOX in tumors are essential to the tumor ’s growth. Though drugs that inhibit ROS production by NOX could be effective against a variety of human cancers, these types of drugs are not widely available.Investigators at the Developmental Therapeutics Branch of the National Cancer Institute (NCI) have synthesized novel analogs of diphenylene iodonium (DPI) and di-thienyl-iodonium (DTI) as inhibitors of NOX and other fla...
Source: NIH OTT Licensing Opportunities - April 14, 2021 Category: Research Authors: ott-admin Source Type: research
Single Domain Antibodies (Nanobodies) Targeting SARS-CoV-2 for treating COVID-19
The COVID-19 pandemic is a worldwide public health crisis with over 100 million confirmed cases and 2.4 million deaths as of February 2021. COVID-19 is caused by a novel coronavirus called SARS-CoV-2. SARS-COV-2 infects hosts via its spike (S) protein. The S protein contains the receptor binding domain (RBD) that binds to the angiotensin converting enzyme 2 (ACE2) receptor on human cells to facilitate viral entry and infection. There are few therapeutics available for COVID-19 patients that directly target SARS-CoV-2.Investigators at the National Cancer Institute (NCI) have isolated a panel of anti-RBD single domain antibo...
Source: NIH OTT Licensing Opportunities - April 5, 2021 Category: Research Authors: ott-admin Source Type: research
Efficacious Fluorinated Cytidine Analog Cancer Therapeutic With Low Toxicity In Animal Studies
Cytidine analogs remain an area of active drug discovery and development, with five FDA approved drugs for the treatment of acute myeloid leukemia (AML). Two of these drugs, azacitidine (Vidaza ®) and decitabine (Dacogen®), which were approved for myelodysplastic syndromes in 2004 and 2006, respectively, inhibit the DNA maintenance methyltransferase DNMT1. Because of the general toxicity of azacitidines, other nucleoside analogs are favored as therapeutics.Researchers in the National Cancer Institute ’sDrug Synthesis and Chemistry Branch recently synthesized a fluorinated cytidine and demonstrated that incorporation of...
Source: NIH OTT Licensing Opportunities - April 5, 2021 Category: Research Authors: ott-admin Source Type: research
