Identification of mRNA expression profiles and their characterization in age-related hearing loss
In this study, we analyzed an mRNA expression dataset (GSE49543) from the GEO database. Differentially expressed genes (DEGs) between young control mice and presbycusis mice were analyzed using limma in R and weighted gene co-expression network analysis (WGCNA) methods. Functional enrichment analyses of the DEGs were conducted with the clusterProfiler R package and the results were visualized using ggplot2 R package. The STRING database was used for the construction of the protein-protein interaction (PPI) network of the screened DEGs. Two machine learning algorithms LASSO and SVM-RFE were used to screen the hub genes. We ...
Source: Cellular and Molecular Biology - April 28, 2024 Category: Molecular Biology Authors: Kanglun Jiang Tan Wang Xinsheng Huang Li Gao Source Type: research
MAL, a potential immunotherapy target, is associated with poor prognosis in cancer patients
In conclusion, the pan-cancer study indicates that MAL with excellent prognostic value is a potential immunotherapy target in multiple cancers.PMID:38678596 | DOI:10.14715/cmb/2024.70.4.39 (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 28, 2024 Category: Molecular Biology Authors: Ruinian Zheng Biao Yao Yongkang Ye Ruijuan Luo Zhiming Wu Xiarong Hu Yinjiao Yuan Xuebing Pan Caixiang Liu Linxuan Huang Yongmei Luo Dan Yang NiYa Shang Yamin Tian Jun Jia Source Type: research
Correlation of IDH1 gene expression error in breast tumor biopsy in patients with invasive ductal carcinoma
This study examined the IDH1 gene expression, suggesting that changes in this gene's expression could impact the prognosis of breast cancer. However, further research is needed to draw definitive conclusions.PMID:38678597 | DOI:10.14715/cmb/2024.70.4.38 (Source: Cellular and Molecular Biology)
Source: Cellular and Molecular Biology - April 28, 2024 Category: Molecular Biology Authors: Behzad Rostami Sepehr Kahrizi Batool Ghorbani Yekta Rezvan Ghadyani Aliasghar Keramatinia Seyed Jalil Hoseini Saeed Karima Abdol Rahim Nikzamir Neda Mansouri Muzi Chen Abolfazl Movafagh Source Type: research
Mechanistic role of pyroptosis in tumor microenvironment and tumor immunotherapy
Cell Mol Biol (Noisy-le-grand). 2024 Apr 28;70(4):260-267. doi: 10.14715/cmb/2024.70.4.41.ABSTRACTIn recent decades, extraordinary attention has been devoted to cell death pathways principally because of multifaceted regulatory roles in normal developmental and pathophysiological processes. The removal of functionally defective, infected or potentially malignant cells is regulated by programmed cell death (PCD) cascades. Pyroptotic cell death is a highly complicated pro-inflammatory form of cell death. Pyroptosis is characterized by the formation of pores in the plasma membrane by oligomerization of the N-terminal fragment...
Source: Cellular and Molecular Biology - April 28, 2024 Category: Molecular Biology Authors: Rukset Attar Muhammad Zahid Qureshi Uteuliyev Yerzhan Sabitaliyevich Ishmuratova Margarita Yulaevna Mirna Azalea Romero Source Type: research
MiR-18 regulates lipid metabolism of non-alcoholic fatty liver disease via IGF1
Cell Mol Biol (Noisy-le-grand). 2024 Apr 28;70(4):237-241. doi: 10.14715/cmb/2024.70.4.37.ABSTRACTWe aimed to illustrate the regulatory effect of miR-18 on the onset of non-alcoholic fatty liver disease (NAFLD). MiR-18 level in liver tissues collected from NAFLD patients and mice was detected. In vivo and in vitro influences of miR-18 on biochemical indexes, glucose tolerance and insulin resistance (IR) in NAFLD were determined. H&E staining was conducted to observe hepatic steatosis in NAFLD mice. The downstream target of miR-18 was finally detected by luciferase assay. MiR-18 was upregulated in liver tissues collecte...
Source: Cellular and Molecular Biology - April 28, 2024 Category: Molecular Biology Authors: Hong Yang Bin Zhang Chun Yu Source Type: research
Comparison of the efficacy and safety of warfarin anticoagulation and left atrial appendage transcatheter occlusion in the treatment of non-valvular atrial fibrillation and investigation of ET-1, hs-CRP and PDGFs
This study compared the therapeutic effect and safety between warfarin anticoagulation and percutaneous left atrial appendage transcatheter occlusion (PLAATO) in non-valvular atrial fibrillation (NVAF). A total of 110 patients were selected and assigned to Control group (n=55) and Observation group (n=55). The control patients were used warfarin, while the observation patients were performed PLAATO. The coagulation function, stroke and bleeding scores were compared between the two groups at different times. Left ventricular function before therapy and 1 year after therapy and adverse events during follow-up were compared b...
Source: Cellular and Molecular Biology - April 28, 2024 Category: Molecular Biology Authors: Yujie Zhang Yali Yao Ling Tao Tao Du Binbin Jia Mi Zhou Zhen Zhang Source Type: research
Tubastatin alleviates epidural fibrosis via negatively targeting TGF β/PI3K/Akt pathway
Cell Mol Biol (Noisy-le-grand). 2024 Apr 28;70(4):231-236. doi: 10.14715/cmb/2024.70.4.36.ABSTRACTEpidural fibrosis (EF) is a chronic, progressive and severe disease. Histone deacetylase 6 (HDAC6) regulates biological signals and cell activities by deacetylating lysine residues and participates in TGF-β-induced epithelial-mesenchymal transition (EMT). Nevertheless, the effect and mechanism of HDAC6 in EF remain unclear. To investigate the effect and mechanism of HDAC6 inhibition on repressing epidural fibrosis. HDAC6 expression and α-smooth muscle actin (α-SMA) in normal human tissue and human EF tissue were assessed by...
Source: Cellular and Molecular Biology - April 28, 2024 Category: Molecular Biology Authors: Lijiang Tao Liangbang Wu Yunlin Ge Source Type: research
Effect and mechanism of Yiqing decoction on hyperuricemia rats
This study aimed to experimentally compare the uric acid-lowering effect and renal protection of Yiqing Fang in a rat model of hyperuricemia. Additionally, we used network pharmacology to predict the potential active components, targets, and pathways of Yiqing Fang. Male SD rats were randomly divided into control, model, Yiqing Fang, allopurinol, and probenecid groups. Serum creatinine (Scr), blood urea nitrogen (BUN), serum uric acid (UA), alanine transaminase (ALT), complete blood count, and urinary NAG enzyme levels were measured. Standard pathology and electron microscopy samples were prepared from the left kidney to o...
Source: Cellular and Molecular Biology - April 28, 2024 Category: Molecular Biology Authors: Yajie Xu Pan Chen Long Sun None Yan Zou None Lixing Zhang None Wanghai Tang None Tingji Zhang Jinlin Huo Jin Zhou Source Type: research
Clinical effect of anlotinib in combination with docetaxel in treating advanced non-small cell lung cancer
This study aimed to explore the clinical performance of anlotinib in combination with docetaxel in treating advanced non-small cell lung cancer (NSCLC). One hundred advanced NSCLC patients admitted to our hospital from January 2019 to December 2022 were retrospectively chosen to be the study objects, and separated into observation group (OG, n=50) and control group (CG, n=50) based on the different drugs used. The CG was given docetaxel injection. The OG was treated with anlotinib hydrochloride capsule combined with docetaxel injection. The clinical effective rate, levels of serum tumor markers, quality of life and occurre...
Source: Cellular and Molecular Biology - April 28, 2024 Category: Molecular Biology Authors: Guibin Weng Weimin Fang Yijin Lin Lin Chen Weikun Su Source Type: research
Characterization of the prognostic, diagnostic, and immunological roles of DSCC1 and its genomic alteration and instability in human cancers
In this study, we obtained data on physiological conditions, transcriptional expression, survival prognosis, genomic alteration, genomic instability, enriched pathways, immune infiltration, and immunotherapy from The Cancer Genome Atlas, The Genotype-Tissue Expression, cBioPortal, and other publicly available databases to systematically characterize the oncogenic and immunological roles of DSCC1 in 33 different cancers. We found that DSCC1 expression was upregulated at both mRNA and protein levels in various cancers. Additionally, DSCC1 expression was associated with higher tumor stage and grade in specific cancers. DSCC1 ...
Source: Cellular and Molecular Biology - April 28, 2024 Category: Molecular Biology Authors: Hailong Liu Zhiwei Cui Rongli Wang Lijun Wang Lihui Wang Feiyan Cheng Rumeng Pan Xinyuan Yang Source Type: research
Effect of circFOXM1/miR-218-5p on the proliferation, apoptosis and migration of glioma cells
This study aimed to explore the influence of circFOXM1/miR-218-5p molecular axis in the proliferation, apoptosis and migration of glioma cells. The levels of circFOXM1 and miR-218-5p in glioma and adjacent tissues were tested by qRT-PCR. Cultured human glioma U251 cells were randomly split into groups: si-NC, si-circFOXM1, miR-NC, miR-218-5p, si-circFOXM1+anti-miR-NC, si-circFOXM1+anti-miR-218-5p. MTT method, plate clone formation, flow cytometry and Transwell experiments were utilized for detecting the proliferation, clone formation, apoptosis and migration of glioma cells. Dual-luciferase reporter experiment authenticate...
Source: Cellular and Molecular Biology - April 28, 2024 Category: Molecular Biology Authors: Renzhi Deng Jianying Chen Jianying Qin Lei Wang Dandan Zhu Xuyong Sun Source Type: research
Drug-coated balloon in the treatment of coronary artery de-novo large lesions angiography
Cell Mol Biol (Noisy-le-grand). 2024 Apr 28;70(4):196-201. doi: 10.14715/cmb/2024.70.4.31.ABSTRACTThe superiority of drug-coated balloon (DCB) in treating small vessels, branching lesions, and high-risk bleeding lesions in coronary heart disease patients has been confirmed. However, its safety and efficacy in large vessels are still unclear. We aimed to investigate whether the efficacy of DCB in large vessels is not inferior to that of drug-eluting stent (DES). From November 2019 to April 2022, a total of 88 patients in our hospital who underwent coronary angiography for the first time and decided to receive DCB or DES tre...
Source: Cellular and Molecular Biology - April 28, 2024 Category: Molecular Biology Authors: Xingyou Cai Xin Hong Yuli Wang Yafei Li Guidong Xu Source Type: research
Effects of miR-129-5p on inflammation and nucleus pulposus cell apoptosis in rats with intervertebral disc degeneration through JNK signaling pathway
This study aimed to explore the effects of miR-129-5p on inflammation and nucleus pulposus (NP) cell apoptosis in rats with intervertebral disc degeneration (IVDD) through the c-Jun N-terminal kinase (JNK) signaling pathway. A total of 20 rats were randomly divided into control group (n=10) or IVDD group (n=10). The mRNA expressions of miR-129-5p and apoptosis index Fas in IVDD tissues were determined using RT-PCR. NP cell apoptosis rate was detected via TUNEL assay. NP cells were extracted from IVDD tissues for primary culture. Subsequently, the cells were transfected with miR-129-5p inhibitor or mimic to inhibit or overe...
Source: Cellular and Molecular Biology - April 28, 2024 Category: Molecular Biology Authors: Chen Li Zhiwen Sun Hongjun Lou Yenong Sun Chengyuan Li Xi Gao Source Type: research
Injectable platelet-rich fibrin promotes proliferation and trichogenic inductivity of dermal papilla cells through activating TGF- β/Smad signaling pathway
Cell Mol Biol (Noisy-le-grand). 2024 Apr 28;70(4):158-163. doi: 10.14715/cmb/2024.70.4.25.ABSTRACTDermal papilla cell (DPC) belongs to a specialized mesenchymal stem cell for hair follicle regeneration. Maintaining the ability of DPCs to stimulate hair in vitro culture is important for hair follicle morphogenesis and regeneration. As the third generation of platelet concentrate, injectable platelet-rich fibrin (i-PRF) is a novel biomaterial containing many growth factors and showing promising effects on tissue reconstruction. We aimed to explore the influences of i-PRF on the proliferative, migratory, as well as trichogeni...
Source: Cellular and Molecular Biology - April 28, 2024 Category: Molecular Biology Authors: Kai Xu Xueqin Deng Minmin Ye Baolin Cheng Lili Zhu Chunming Ding Tuanjie Hou Source Type: research
Monitoring and analysis of pathogenic microorganisms and resistance genes
The objectives are to improve the rapid identification method of microbial risk and cut off the route of transmission of resistance genes. When new pathogenic microorganisms are found, intervention can be carried out as early as possible to identify the risk of potential pathogen transmission, and timely cut off the transmission route. Hospital air samples were collected to analyse the distribution of environmental pathogenic microorganisms and the characteristics of ARGs resistance genes. The air samples were collected from 12 sampling sites in the Affiliated Hospital of Yangzhou University. In the infusion room, general ...
Source: Cellular and Molecular Biology - April 28, 2024 Category: Molecular Biology Authors: Min Zhang Guimei Kong Yang Li Yuehan Li Yan Xu Source Type: research
