Comparison of two acquisition modes in mass spectrometry for quantification of 12 antibiotics, including beta-lactams, linezolid, fluoroquinolones and clindamycin: Multiple reactions monitoring (MRM) and fullMS/dd-MS < sup > 2 < /sup >
Anal Biochem. 2024 Mar 19;690:115507. doi: 10.1016/j.ab.2024.115507. Online ahead of print.ABSTRACTThe quantification of antibiotics, using mass spectrometry, for monitoring therapeutic drugs is a key benefit in infection management. After an easy work-up of plasma samples, analysis were performed using both two widely used acquisition modes: MRM for the triple quadrupole spectrometer and fullMS/ddMS2 for the HRMS to quantify twelve antibiotics. Comparison between the two acquisition modes were performed. Validation parameters and sample values were used as comparison criteria. The results indicated a good correlation betw...
Source: Analytical Biochemistry - March 21, 2024 Category: Biochemistry Authors: Catherine Feliu Celine Konecki Damien Vautier Jessica Rogier Claire Gozalo Yoann Cazaubon Zoubir Djerada Source Type: research
Comparison of two acquisition modes in mass spectrometry for quantification of 12 antibiotics, including beta-lactams, linezolid, fluoroquinolones and clindamycin: Multiple reactions monitoring (MRM) and fullMS/dd-MS < sup > 2 < /sup >
Anal Biochem. 2024 Mar 19;690:115507. doi: 10.1016/j.ab.2024.115507. Online ahead of print.ABSTRACTThe quantification of antibiotics, using mass spectrometry, for monitoring therapeutic drugs is a key benefit in infection management. After an easy work-up of plasma samples, analysis were performed using both two widely used acquisition modes: MRM for the triple quadrupole spectrometer and fullMS/ddMS2 for the HRMS to quantify twelve antibiotics. Comparison between the two acquisition modes were performed. Validation parameters and sample values were used as comparison criteria. The results indicated a good correlation betw...
Source: Analytical Biochemistry - March 21, 2024 Category: Biochemistry Authors: Catherine Feliu Celine Konecki Damien Vautier Jessica Rogier Claire Gozalo Yoann Cazaubon Zoubir Djerada Source Type: research
Characterization of Weissella Species during a 3-Year Observational Study - An Emerging Threat
In this study, we aimed to evaluate the demographic pattern, presenting symptoms, risk factors, associated pathologies, and clinical outcomes in patients with Weissella infection. We also analyzed the antibiotic susceptibility pattern of the Weissella species isolated. This retrospective observational study was done from January 2021 to August 2023 in a tertiary care referral center in Uttar Pradesh. All Weissella species isolated from blood cultures or cerebrospinal fluid (CSF) samples during this period were included in the study. Twenty-four-hour growth from a positive blood culture or CSF sample was identified by matri...
Source: The American Journal of Tropical Medicine and Hygiene - March 20, 2024 Category: Tropical Medicine Authors: Ashima Jamwal Gerlin Varghese Deepika Sarawat Nidhi Tejan Sangram Singh Patel Chinmoy Sahu Source Type: research
Characterization of Weissella Species during a 3-Year Observational Study - An Emerging Threat
In this study, we aimed to evaluate the demographic pattern, presenting symptoms, risk factors, associated pathologies, and clinical outcomes in patients with Weissella infection. We also analyzed the antibiotic susceptibility pattern of the Weissella species isolated. This retrospective observational study was done from January 2021 to August 2023 in a tertiary care referral center in Uttar Pradesh. All Weissella species isolated from blood cultures or cerebrospinal fluid (CSF) samples during this period were included in the study. Twenty-four-hour growth from a positive blood culture or CSF sample was identified by matri...
Source: Am J Trop Med Hyg - March 20, 2024 Category: Infectious Diseases Authors: Ashima Jamwal Gerlin Varghese Deepika Sarawat Nidhi Tejan Sangram Singh Patel Chinmoy Sahu Source Type: research
Characterization of Weissella Species during a 3-Year Observational Study - An Emerging Threat
In this study, we aimed to evaluate the demographic pattern, presenting symptoms, risk factors, associated pathologies, and clinical outcomes in patients with Weissella infection. We also analyzed the antibiotic susceptibility pattern of the Weissella species isolated. This retrospective observational study was done from January 2021 to August 2023 in a tertiary care referral center in Uttar Pradesh. All Weissella species isolated from blood cultures or cerebrospinal fluid (CSF) samples during this period were included in the study. Twenty-four-hour growth from a positive blood culture or CSF sample was identified by matri...
Source: The American Journal of Tropical Medicine and Hygiene - March 20, 2024 Category: Tropical Medicine Authors: Ashima Jamwal Gerlin Varghese Deepika Sarawat Nidhi Tejan Sangram Singh Patel Chinmoy Sahu Source Type: research
Characterization of Weissella Species during a 3-Year Observational Study - An Emerging Threat
In this study, we aimed to evaluate the demographic pattern, presenting symptoms, risk factors, associated pathologies, and clinical outcomes in patients with Weissella infection. We also analyzed the antibiotic susceptibility pattern of the Weissella species isolated. This retrospective observational study was done from January 2021 to August 2023 in a tertiary care referral center in Uttar Pradesh. All Weissella species isolated from blood cultures or cerebrospinal fluid (CSF) samples during this period were included in the study. Twenty-four-hour growth from a positive blood culture or CSF sample was identified by matri...
Source: Am J Trop Med Hyg - March 20, 2024 Category: Infectious Diseases Authors: Ashima Jamwal Gerlin Varghese Deepika Sarawat Nidhi Tejan Sangram Singh Patel Chinmoy Sahu Source Type: research
Characterization of Weissella Species during a 3-Year Observational Study - An Emerging Threat
In this study, we aimed to evaluate the demographic pattern, presenting symptoms, risk factors, associated pathologies, and clinical outcomes in patients with Weissella infection. We also analyzed the antibiotic susceptibility pattern of the Weissella species isolated. This retrospective observational study was done from January 2021 to August 2023 in a tertiary care referral center in Uttar Pradesh. All Weissella species isolated from blood cultures or cerebrospinal fluid (CSF) samples during this period were included in the study. Twenty-four-hour growth from a positive blood culture or CSF sample was identified by matri...
Source: The American Journal of Tropical Medicine and Hygiene - March 20, 2024 Category: Tropical Medicine Authors: Ashima Jamwal Gerlin Varghese Deepika Sarawat Nidhi Tejan Sangram Singh Patel Chinmoy Sahu Source Type: research
Characterization of Weissella Species during a 3-Year Observational Study - An Emerging Threat
In this study, we aimed to evaluate the demographic pattern, presenting symptoms, risk factors, associated pathologies, and clinical outcomes in patients with Weissella infection. We also analyzed the antibiotic susceptibility pattern of the Weissella species isolated. This retrospective observational study was done from January 2021 to August 2023 in a tertiary care referral center in Uttar Pradesh. All Weissella species isolated from blood cultures or cerebrospinal fluid (CSF) samples during this period were included in the study. Twenty-four-hour growth from a positive blood culture or CSF sample was identified by matri...
Source: Am J Trop Med Hyg - March 20, 2024 Category: Infectious Diseases Authors: Ashima Jamwal Gerlin Varghese Deepika Sarawat Nidhi Tejan Sangram Singh Patel Chinmoy Sahu Source Type: research
Characterization of Weissella Species during a 3-Year Observational Study - An Emerging Threat
In this study, we aimed to evaluate the demographic pattern, presenting symptoms, risk factors, associated pathologies, and clinical outcomes in patients with Weissella infection. We also analyzed the antibiotic susceptibility pattern of the Weissella species isolated. This retrospective observational study was done from January 2021 to August 2023 in a tertiary care referral center in Uttar Pradesh. All Weissella species isolated from blood cultures or cerebrospinal fluid (CSF) samples during this period were included in the study. Twenty-four-hour growth from a positive blood culture or CSF sample was identified by matri...
Source: The American Journal of Tropical Medicine and Hygiene - March 20, 2024 Category: Tropical Medicine Authors: Ashima Jamwal Gerlin Varghese Deepika Sarawat Nidhi Tejan Sangram Singh Patel Chinmoy Sahu Source Type: research
Characterization of Weissella Species during a 3-Year Observational Study - An Emerging Threat
In this study, we aimed to evaluate the demographic pattern, presenting symptoms, risk factors, associated pathologies, and clinical outcomes in patients with Weissella infection. We also analyzed the antibiotic susceptibility pattern of the Weissella species isolated. This retrospective observational study was done from January 2021 to August 2023 in a tertiary care referral center in Uttar Pradesh. All Weissella species isolated from blood cultures or cerebrospinal fluid (CSF) samples during this period were included in the study. Twenty-four-hour growth from a positive blood culture or CSF sample was identified by matri...
Source: Am J Trop Med Hyg - March 20, 2024 Category: Infectious Diseases Authors: Ashima Jamwal Gerlin Varghese Deepika Sarawat Nidhi Tejan Sangram Singh Patel Chinmoy Sahu Source Type: research
Characterization of Weissella Species during a 3-Year Observational Study - An Emerging Threat
In this study, we aimed to evaluate the demographic pattern, presenting symptoms, risk factors, associated pathologies, and clinical outcomes in patients with Weissella infection. We also analyzed the antibiotic susceptibility pattern of the Weissella species isolated. This retrospective observational study was done from January 2021 to August 2023 in a tertiary care referral center in Uttar Pradesh. All Weissella species isolated from blood cultures or cerebrospinal fluid (CSF) samples during this period were included in the study. Twenty-four-hour growth from a positive blood culture or CSF sample was identified by matri...
Source: The American Journal of Tropical Medicine and Hygiene - March 20, 2024 Category: Tropical Medicine Authors: Ashima Jamwal Gerlin Varghese Deepika Sarawat Nidhi Tejan Sangram Singh Patel Chinmoy Sahu Source Type: research
Characterization of Weissella Species during a 3-Year Observational Study - An Emerging Threat
In this study, we aimed to evaluate the demographic pattern, presenting symptoms, risk factors, associated pathologies, and clinical outcomes in patients with Weissella infection. We also analyzed the antibiotic susceptibility pattern of the Weissella species isolated. This retrospective observational study was done from January 2021 to August 2023 in a tertiary care referral center in Uttar Pradesh. All Weissella species isolated from blood cultures or cerebrospinal fluid (CSF) samples during this period were included in the study. Twenty-four-hour growth from a positive blood culture or CSF sample was identified by matri...
Source: Am J Trop Med Hyg - March 20, 2024 Category: Infectious Diseases Authors: Ashima Jamwal Gerlin Varghese Deepika Sarawat Nidhi Tejan Sangram Singh Patel Chinmoy Sahu Source Type: research
Synthesis of Pharmaceutically Relevant Arylamines Enabled by a Nitroreductase from Bacillus tequilensis
In this study, we assessed a novel NR from Bacillus tequilensis, named BtNR, for the synthesis of pharmaceutically relevant arylamines, including valuable synthons used in the manufacture of blockbuster drugs such as vismodegib, sonidegib, linezolid and sildenafil. After optimizing the enzymatic reaction conditions, high conversion of nitroaromatics to arylamines (up to 97%) and good product yields (up to 56%) were achieved. Our results indicate that BtNR has a broad substrate scope, including bulky nitro benzenes, nitro pyrazoles and nitro pyridines. Hence, BtNR is an interesting biocatalyst for the synthesis of pharmaceu...
Source: Chembiochem - March 19, 2024 Category: Biochemistry Authors: Sara Russo Alejandro Prats Luj án Marco Fraaije Gerrit J Poelarends Source Type: research
Use of multiple pharmacodynamic measures to deconstruct the Nix-TB regimen in a short-course murine model of tuberculosis
Antimicrob Agents Chemother. 2024 Mar 19:e0101023. doi: 10.1128/aac.01010-23. Online ahead of print.ABSTRACTA major challenge for tuberculosis (TB) drug development is to prioritize promising combination regimens from a large and growing number of possibilities. This includes demonstrating individual drug contributions to the activity of higher-order combinations. A BALB/c mouse TB infection model was used to evaluate the contributions of each drug and pairwise combination in the clinically relevant Nix-TB regimen [bedaquiline-pretomanid-linezolid (BPaL)] during the first 3 weeks of treatment at human equivalent doses. The...
Source: Antimicrobial Agents and Chemotherapy - March 19, 2024 Category: Microbiology Authors: M A Lyons A Obregon-Henao M E Ramey A A Bauman S Pauly K Rossmassler J Reid B Karger N D Walter G T Robertson Source Type: research
Synthesis of Pharmaceutically Relevant Arylamines Enabled by a Nitroreductase from Bacillus tequilensis
In this study, we assessed a novel NR from Bacillus tequilensis, named BtNR, for the synthesis of pharmaceutically relevant arylamines, including valuable synthons used in the manufacture of blockbuster drugs such as vismodegib, sonidegib, linezolid and sildenafil. After optimizing the enzymatic reaction conditions, high conversion of nitroaromatics to arylamines (up to 97%) and good product yields (up to 56%) were achieved. Our results indicate that BtNR has a broad substrate scope, including bulky nitro benzenes, nitro pyrazoles and nitro pyridines. Hence, BtNR is an interesting biocatalyst for the synthesis of pharmaceu...
Source: Chembiochem - March 19, 2024 Category: Biochemistry Authors: Sara Russo Alejandro Prats Luj án Marco Fraaije Gerrit J Poelarends Source Type: research
